FastQCFastQC Report
Thu 26 May 2016
SRR2099155_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099155_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131840
Sequences flagged as poor quality0
Sequence length42
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCTGCAGTCAGCCCGACAACGGCTGCCCGTTGGTGGGGGA8170.6196905339805825No Hit
CTGATGCAGCCTGAGGCGATCGTCGGACTGTAGAACTCTGAA6140.46571601941747576Illumina DpnII expression Sequencing Primer (100% over 25bp)
CTGATGCGGCAATCCGGGCTTCACCGCGACGTGCTCGAACAG6080.4611650485436893No Hit
CTGGTGCTGCCTGAGGCGATCGTCGGACTGTAGAACTCTGAA3450.26168082524271846Illumina DpnII expression Sequencing Primer (100% over 25bp)
CTGATGCAGAAAAGGTGGCCTCCACCTCCACCAGCCCAGCTT3290.24954490291262135No Hit
CTGATTGATATTCGCTTTCGCCCTGCCAGCCGACTTCCTGCA2700.20479368932038836No Hit
CTGATGTAACAACAAACTGGAGTTCCCATTGTGGTGCAGCAG2080.15776699029126212No Hit
CTGATGCCGAAGCCCCCGCAGGGCTGTGGGCAGGAACCCTGG2030.1539745145631068No Hit
CTGATGCACACTGAAAGAAGGTGGATGCCAAGAAGCAGAAGC2010.15245752427184467No Hit
CTGATGCACCACACGATCCCCTCTTTATCTTCTTAAGTTAGT1870.1418385922330097No Hit
CTGATGCAAGTTTACAGTCCGACGATCGGCGCTGATGCATCA1810.1372876213592233No Hit
CTGATGCATTAAGGGCAATAACCCACCCCCCCATAGAGGCTG1780.1350121359223301No Hit
CTGACGCTCCTCGCTCAGCGCAAGCCTAATCGAATTGCATCG1770.13425364077669905No Hit
CTGATGCACGCTGATGGGGATCGGGGATTGCAATTATTCCCC1650.12515169902912623No Hit
CTGATGCAACGTTGGGGATGATGGAGTCTGCTTGAATGAATT1630.12363470873786409No Hit
CTGATGCACGTCGAATTGTGGGGGCAATCTAGCTGCATTAAG1570.11908373786407767No Hit
CTGATGCATAGTGGGAACTGTGGGGCTGGTCCTGGTTGGTGG1510.11453276699029127No Hit
CTGACGCTCCTCCTCAGCGCAAGCCTAATCGAATTGCATCGG1480.11225728155339805No Hit
CTGATGCACCGTGGAAAGGAAGACTAGCCACCTGACTTGGTC1430.1084648058252427No Hit
CTGATGCATACCGTGGCAACGCAAGGGAGGCTGGGCCGGCCG1340.10163834951456312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAC309.943888E-636.009876
GTCGAAT309.943888E-636.0098710
CAACGTT309.943888E-636.009877
CAACGTC309.943888E-636.009877
GCAACCC356.9017005E-736.009876
GCAAATG309.943888E-636.009876
CACGCGT453.3669494E-936.009877
GCACGAC453.3669494E-936.009876
TGCATAA1200.036.009875
GCATTGT356.9017005E-736.009876
TGCGAGA601.8189894E-1236.009875
CAGTGCG309.943888E-636.009877
ATGACGG309.943888E-636.009878
CATACCG356.9017005E-736.009877
CGTCGAA309.943888E-636.009879
CAATGGG309.943888E-636.009877
GCAATCG650.036.009876
GCAATAC1400.036.009876
CACACCG356.9017005E-736.009877
CACGTCG601.8189894E-1236.009877
Produced by FastQC (version 0.11.5)