FastQCFastQC Report
Thu 26 May 2016
SRR2099154_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099154_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363154
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTGCAATTAGAAGCTAGCAGCTGATCACCAGCTTGCCGGC35840.9869091349675344No Hit
AGTCAGCAGATCACTTCGTCACCAGTCGGGCGAACGGGCTGGG24710.6804275871944133No Hit
AGTCTGCAGCGAAAGTGCACGAAGGGATCGGCCGCACCGGCGT23010.6336154909487435No Hit
AGTCAGCAGATCAGCTTCGTCACCAGTCGGGCGAACGGGCTGG11630.3202498113747887No Hit
TGTCTTCATACGAGTAATCTGTATTCGTTTTTTAGAGAGCTTA10100.27811892475368577No Hit
AGTCTGCAAAGATGGAAGCTGTGCTTGTAAAGAAAAGAAACCG9860.2715101582248853No Hit
AGTCTGCAGAGGGCGTGAATGTCAACGGCTACGTGCACTCCAA9080.2500316670062838No Hit
AGTCAGCAGGCGCTCCTGGGGCTGAAGTAGGTCCCAAGGGTAT8530.23488657704444946No Hit
AGTCTGCAGACTTCTGGTGGTTGCATCCAAGTATCTGTCTTTC8150.2244226967071821No Hit
AGTCTGCAATTGCGGTATACATATGTCAGCATAACAGACACAT7850.2161617385461815No Hit
AGTCTCCAAACGCAGGTGTTTTGGGACTACGGTGGAAATTATG7170.19743690004791356No Hit
AATCTGCGTAAGCATGGTTTTAATCGCACTGACTATTTTGGCA6830.1880744807987796No Hit
AGTCTGCATCTCGGTCCGACGATCTTGATAGTCTGACTGTAGA6730.18532082807844608No Hit
AGTCTGCAAGCGGGTCCGACGATCAAAATAGTCTGACTGTAGA6330.17430621719711198No Hit
AGTATGCAATCGAGTCGGTAACGAACTGGTCAATGGCGGTTCT6040.16632062430814476No Hit
AGTCTGCATCTCGTGTTCCCGCCATAATGATTACTGACACCGC5950.1638423368598446No Hit
AGTCGGCTCTATCCGCATCGTCGCCATGGCCGGAGACGGCATC5890.16219014522764447No Hit
AGTCCGCAGGGGGTCGGAATTCAGACCTGATAAGGGATTGTAT5490.15117553434631037No Hit
AGTCAGCAGATCATCTTCGTCACCAGTCGGGCGAACGGGCTGG5400.14869724689801023No Hit
AGTCTGCACCGGGGTCCGACGATCTTGATAGTCTGACTGTAGA5270.1451174983615766No Hit
AGTCTGTATGGACGCGAGATCTGATAGTTTTATCAAGGGTTTC5200.14318994145734318No Hit
AGTGGGCATCGACGTTTTGATTGAATAGCAAACATGTGCTGAA5050.13905946237684289No Hit
AGTCTGCAAAGATGGGAAGCTGTGCTTGTAAAGAAAAGAAACC5030.13850873183277618No Hit
TGTCTCCACATGTGTCCGACGATCCATGAGGTAGTCGTACTGT4980.1371319054726094No Hit
AGTCTGTATGGACCGAGATCTGATAGTTTTATCAAGGGTTTCT4720.12997240839974228No Hit
AGTCTGCATCTATCTCCAAGCGCCAGGCGTGGACGCTTGAAGG4520.12446510295907522No Hit
AGTCTGCTACCGAGTTTTTCTCCCAAAATAAGAAAACAACAGA4370.12033462387857494No Hit
AGCCTGCTATCACGCTTCGGGGCCTGCACTTCCCGGCAAACTG4350.11978389333450823No Hit
TGTCTCCACATGTGGTCCGACGATCCATGAGGTAGTCGTACTG4130.11372585734977447No Hit
AGTCTGCATCTCTGGTAGGACGTGGAATGTGGCAAGAAAACTG4090.11262439626164107No Hit
AGTCTGTATTCTATTGCCATCACCAGACTTTGATAAATCTCGG3860.10629099500487396No Hit
AGTCTGCAGACAAAAGGAGGAGATCAGGAGACAGGAGTTTCTT3790.10436343810064048No Hit
AGTATGTAGGATCGGGATTACAGGCATGCACCACCACGCCAGC3660.10078368956420693No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCG200.001793408837.19484736
TAAAGAC200.001838999137.00523413
CGCTTCG403.8162398E-837.00523413
ACAACGT251.2319352E-437.0001378
AACCGCA200.001840243937.00013710
CCGGGTG200.001840243937.0001379
ACCGGGT403.8207872E-837.0001378
ACTGCGC251.2319352E-437.0001373
TACCACG200.001840243937.00013711
CTATCTA251.2319352E-437.00013711
ACGTTGT251.2319352E-437.0001378
CATGCGG200.001840243937.0001377
CATGCGA355.6169847E-737.0001377
GCGGTAT750.037.00013712
GCAACGG501.7826096E-1037.0001376
GCACGTT700.037.0001376
GCAAACC551.2732926E-1137.0001376
GCACGCG1950.037.0001376
CCTGTTG200.001840243937.0001379
CAATTAT251.2319352E-437.0001377
Produced by FastQC (version 0.11.5)