FastQCFastQC Report
Thu 26 May 2016
SRR2099153_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099153_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111874
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTGCAATTAGAAGCTAGCAGCTGATCACCAGCTTGCCGGC10030.896544326653199No Hit
AGTCTGCAGCGAAAGTGCACGAAGGGATCGGCCGCACCGGCGT7370.6587768382287216No Hit
AGTCAGCAGATCACTTCGTCACCAGTCGGGCGAACGGGCTGGG6930.6194468777374547No Hit
TGTCTTCATACGAGTAATCTGTATTCGTTTTTTAGAGAGCTTA3280.29318697820762646No Hit
AGTCTGCAGAGGGCGTGAATGTCAACGGCTACGTGCACTCCAA3180.28424835082324756No Hit
AGTCAGCAGATCAGCTTCGTCACCAGTCGGGCGAACGGGCTGG3160.28246062534637184No Hit
AGTCTGCAAAGATGGAAGCTGTGCTTGTAAAGAAAAGAAACCG3110.2779913116541824No Hit
AGTCAGCAGGCGCTCCTGGGGCTGAAGTAGGTCCCAAGGGTAT2590.23151044925541234No Hit
AGTCTGCAGACTTCTGGTGGTTGCATCCAAGTATCTGTCTTTC2220.19843752793321057No Hit
AGTCTGCAATTGCGGTATACATATGTCAGCATAACAGACACAT2100.18771117507195595No Hit
AGTCTCCAAACGCAGGTGTTTTGGGACTACGGTGGAAATTATG1950.17430323399538766No Hit
AGTCGGCTCTATCCGCATCGTCGCCATGGCCGGAGACGGCATC1900.16983392030319824No Hit
AGTCTGCATCTCGGTCCGACGATCTTGATAGTCTGACTGTAGA1870.1671523320878846No Hit
AGTCTGCAAGCGGGTCCGACGATCAAAATAGTCTGACTGTAGA1820.16268301839569516No Hit
AGTATGCAATCGAGTCGGTAACGAACTGGTCAATGGCGGTTCT1740.15553211648819207No Hit
AGTCTGCATCTCGTGTTCCCGCCATAATGATTACTGACACCGC1730.1546382537497542No Hit
AATCTGCGTAAGCATGGTTTTAATCGCACTGACTATTTTGGCA1660.14838121458068898No Hit
AGTCTGCAAAGATGGGAAGCTGTGCTTGTAAAGAAAAGAAACC1570.14033644993474803No Hit
AGTCAGCAGATCATCTTCGTCACCAGTCGGGCGAACGGGCTGG1540.13765486171943436No Hit
AGTCTGCAGACAGGTGCCCCCTCTCTGTGTGTATGCAGTGCTG1500.13407941076568283No Hit
AGTCCGCAGGGGGTCGGAATTCAGACCTGATAAGGGATTGTAT1470.13139782255036916No Hit
AGTCTGCACCAGTGGAGTCCCCCAGTCTCTGTCTGTAGCTCTG1450.12961009707349339No Hit
TGTCTCCACATGTGTCCGACGATCCATGAGGTAGTCGTACTGT1410.12603464611974186No Hit
AGTGGGCATCGACGTTTTGATTGAATAGCAAACATGTGCTGAA1370.12245919516599031No Hit
AGTCTGCATGGTTGTGGCCGACTGGTCTGAGGGTGTGCAGGTT1370.12245919516599031No Hit
AGTCTGTATGGACGCGAGATCTGATAGTTTTATCAAGGGTTTC1340.11977760695067664No Hit
AGTCTGCATCTCTGGTAGGACGTGGAATGTGGCAAGAAAACTG1330.11888374421223877No Hit
AGTCTGTATGGACCGAGATCTGATAGTTTTATCAAGGGTTTCT1280.11441443052004935No Hit
AGTCTGCACCGGGGTCCGACGATCTTGATAGTCTGACTGTAGA1260.11262670504317357No Hit
AGTCTGCACACTACAGTCCGACGATCCTTGCAGTCTGCGCGGA1220.10905125408942204No Hit
TGTCTCCACATGTGGTCCGACGATCCATGAGGTAGTCGTACTG1210.10815739135098415No Hit
TGTCTTCATACGAGGTAATCTGTATTCGTTTTTTAGAGAGCTT1170.10458194039723259No Hit
AGCCTGCTATCACGCTTCGGGGCCTGCACTTCCCGGCAAACTG1140.10190035218191895No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGAC200.00183484237.012
CTATGTG200.00183484237.010
AGACTTG452.575689E-937.08
AGGTATC403.7805876E-837.08
TCGTTGC200.00183484237.025
CAAACGC452.575689E-937.07
ACCGGGG501.7644197E-1037.08
GCAAGGT308.245041E-637.06
GAGCTTA251.2265143E-437.037
GCGACAT200.00183484237.036
CAAGTTA308.245041E-637.07
TCCGCAT200.00183484237.012
AGTTTCG355.570946E-737.08
ATTAGAA1450.037.09
GTACACT200.00183484237.031
AAGAGCG251.2265143E-437.08
CACGGCG251.2265143E-437.07
CAACGCT200.00183484237.07
CGACGGT200.00183484237.010
GCGGTAT251.2265143E-437.012
Produced by FastQC (version 0.11.5)