SRR2099151_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2099151_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	105799
Sequences flagged as poor quality	0
Sequence length	43
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTCACTTAGGTTAATCGTCGGCTGGTAGCCGTCGAGCAGGTGA	628	0.5935783892097279	No Hit
GTCACTAGTCTATAAAGGAACAAAGAAGACCTCCCACTAGGAA	624	0.5897976351383284	No Hit
GTCACTAGCTGGGAGGGAGGCAATGAAAGTTCTCAATCAACAA	488	0.461251996710744	No Hit
GTCACTAGTGTCGAAGCACAATTAGAGCACCAAACCAGGCCAA	369	0.34877456308660765	No Hit
GTCACTAGGTTTGCACAGGAGGGGAGGAATCTGGGTAATGCTT	320	0.3024603257119633	No Hit
GACGCTAGTCGATGTGACATACTTTCCTTTTGGTAAAGCAAAG	248	0.23440675242677153	No Hit
GTCACTAGCGAGTGGTTCATGGAACAGAATAGAAAGACCAGAA	199	0.18809251505212715	No Hit
GTCACTAGGATCTATCGGAGTGTCACTAGTTTTTTTTTTTTTT	176	0.1663531791415798	No Hit
GTCACTAGCTTCGCACAGCTGTTCCAATGAGCGATCTTGCCCT	169	0.15973685951663058	No Hit
GTCACTAGGAGAAATGAAGTGAAGTGAACTGATGAAGAAACTG	166	0.15690129396308095	No Hit
GTCACTAGCTATACGGTGCGTTTTGGTGGATTCTGCAAGGATA	166	0.15690129396308095	No Hit
GTCCCTAGGTGGGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT	165	0.15595610544523106	No Hit
GTCACCTGCCAATGAGGAGAAGACCAAGGACCAGCCCTTTGAT	164	0.15501091692738117	No Hit
GTCACCCGGTGGATGTTTGGTACATCATACAATCCTAACAATT	153	0.14461384323103244	No Hit
GTCACTAGCGAGGGATCGCCGCAGGCCGCCGCGCTTATGTGCA	149	0.14083308915963288	No Hit
GTCACTAGAGAGACAGTCCGACGATCCGCTTGTCACTAGTGAC	145	0.13705233508823333	No Hit
GTCACTAGGAAGGGTAGCAGGAGTGGCAGACTGGCCAAGCAGG	137	0.12949082694543426	No Hit
GTCACTAGTTCGTACAGTCCGACGATCAATAAGTCACTAGTGA	129	0.12192931880263519	No Hit
GTCACCCGGTGGAGTTTGGTACATCATACAATCCTAACAATTT	129	0.12192931880263519	No Hit
GTCACTAGTTAGGGTGCCTATGATTTTTGCAGTAGCTTGCTTG	126	0.11909375324908553	No Hit
GTCACTAGTTGATGAGAGCATGCTAACTGATTATCCAATGTCA	120	0.11342262214198622	No Hit
GTCACTAGCCCATGATGCCAGCCTTTCTACAATCAACTTGGAC	119	0.11247743362413634	No Hit
GTCACTAGTTTATGCGCAGCAGCGAGGTGAAGACGCGGAAAGG	118	0.11153224510628645	No Hit
GTCACTAGTTTATGGCGCAGCAGCGAGGTGAAGACGCGGAAAG	117	0.11058705658843658	No Hit
GTCACCCGGTGGAGTATGATGTTTGGTACATCATACAATCCTA	114	0.1077514910348869	No Hit
GTCACTAGGGTCTACAGTCCGACGATCCGAAAGTCACTAGTGA	113	0.10680630251703703	No Hit
GTCACTAATCTCTACGTGCGCGAGTCGGGGGCTCGCACGAAAG	112	0.10586111399918713	No Hit
GTCACTAGACATTCAGTCCGACGATCGATCGGTCACTAGTGAC	110	0.10397073696348737	No Hit
GACGCTAGTCGATAGTGACATACTTTCCTTTTGGTAAAGCAAA	108	0.1020803599277876	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGGCGC	20	0.0018256427	37.035988	14
ATCGGTG	20	0.0018256427	37.035988	13
TGGTTCA	35	5.52478E-7	37.035984	13
GTCGAAG	35	5.545335E-7	37.01846	10
ATGGAAC	35	5.545335E-7	37.01846	19
GCTATAT	35	5.545335E-7	37.01846	8
GGGACCG	35	5.545335E-7	37.01846	8
TATACGG	35	5.545335E-7	37.01846	10
AGCACAA	30	8.212561E-6	37.018456	15
GGAATCG	25	1.2224601E-4	37.018456	8
TCGCACA	20	0.0018298952	37.018456	11
ACACGCC	20	0.0018298952	37.018456	19
GTCGATG	45	2.559318E-9	37.018456	8
CGTATAG	20	0.0018298952	37.018456	9
GAGCTCG	20	0.0018298952	37.018456	8
GGCACAC	20	0.0018298952	37.018456	16
GTTTATC	60	0.0	37.018456	8
GACCGAA	20	0.0018298952	37.018456	10
GAATCGA	25	1.2224601E-4	37.018456	9
GCACGCT	25	1.2224601E-4	37.018456	8

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content