SRR2099150_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2099150_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	375394
Sequences flagged as poor quality	0
Sequence length	43
%GC	47

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCATTCGACGCGGGTTCTTCGACCGGGCCGTCGAGGAAGGCCT	4876	1.2989019536806663	No Hit
GCATTCGAGCGGAGGAGAATTGCCTGCTGTCCTGCTGTAAACT	2521	0.6715610798254634	No Hit
GCATTCGAGACAGGGTTACGGTACCGCGCGCCCTGATCGCCGA	2388	0.6361316376926642	No Hit
GCATTCGAGCCCTTCGGGATGTTGCTGACGCCGCCGGATGTCT	1594	0.4246205320276829	No Hit
GCATTCGAGACAGGGGTTACGGTACCGCGCGCCCTGATCGCCG	1590	0.4235549848958694	No Hit
GCATTCGAGGTGAGCCTTCACAGTGAGCTATCCCAAAGCTCAG	1560	0.4155633814072681	No Hit
GCATCCGCGTCCGGGTGCTTCACCCATTCGGGCATGCCCTGCA	1244	0.331385157994001	No Hit
GCATTCGAGGAACCAAGAACACAACATAGCAGGAAGTATTCAA	1141	0.3039473193498031	No Hit
GCATTCGAATCACCGTTTATTGCAGCTCTTACCATTTCCCTAG	1117	0.2975540365589221	No Hit
GCATTCGACGCGGGGTTCTTCGACCGGGCCGTCGAGGAAGGCC	1088	0.2898288198532742	No Hit
GCATTCGACCAGTAGTCCGACGATCTGAGCGCATTCGATTTTT	1050	0.27970612210104584	No Hit
GCATTCGAATTTCGCAGAATCATCATATTCTAGGAATTATAGA	1000	0.266386782953377	No Hit
GCATTCGATCTCTGTCTTAAGGAGCGCGGCGATGCTTGAAATG	808	0.2152405206263286	No Hit
GCATTCGAACGCTGAGTTCTAGAGTCGGACGATCGTTTAGCAT	755	0.20112202112979963	No Hit
GCACTCGAGGGGCAAGTGATCGACTCGAAGCAAACAACCAACC	682	0.1816757859742031	No Hit
GCAACCGAGTACAGTCAACTGCTAGTTTTTTTTTTTTTTTTTT	615	0.16382787151632683	No Hit
GCATCTGAAGTTGGACCTTTCTCAGCATGGCTTACATCTTAGC	587	0.1563690415936323	No Hit
GCATTCGAATCACGCTATGGGAACTGTGTTACCAGAGGAAAAT	580	0.15450433411295864	No Hit
GCATTCGAGCTTGACATAACTCCAACATCATCAACCTCATACA	544	0.1449144099266371	No Hit
GCATTCGATGTGTGTTCTACAGTCCGACGATCCGCGAGCATTC	531	0.14145138174824318	Illumina DpnII expression Sequencing Primer (95% over 21bp)
GCATTCGTTCAACAGCCACGTTTGGCGTATAGCGTTTCAGCAG	513	0.1366564196550824	No Hit
GCATTCGAATAGTAGGTGGCTTTCTGAGTTCCAGTCCAGCCTG	511	0.13612364608917565	No Hit
GCATTCAAGCGCGGCATTCGATTTTTTTTTTTTTTTTTTTTTT	510	0.13585725930622225	No Hit
GCATTCGAACCAAGGCGAGAAAACTGAAAATCACGGAAAATGA	498	0.13266061791078174	No Hit
GCATTCGACGGTACCAAAACTGAGTGATAGAGGCAGGAAGCTC	493	0.13132868399601486	No Hit
GAATTCGAGCAGTGCTGACTGAGCGTCCACTTCTGTTAACATA	487	0.1297303632982946	No Hit
GCATTCGAGCAAAGTCCGACGATCCGAGAGCATTCGATTTTTT	462	0.12307069372446017	No Hit
GCATTCGACATTGTGCAGCTCCCAGGACAGAGGCCCCACTCTT	452	0.12040682589492639	No Hit
GCATTCAATATTACTGTGGTGTTTCACTGCAGGGACCTCTAGT	438	0.11667741093357913	No Hit
GCATTCGAACAGAGGCGAGGAAAACTGAAAAAGGTAGAAAATG	437	0.11641102415062574	No Hit
GCATTCGACAATGGGCGTCTGAAGGCAAGATGGGTCACCAGCA	409	0.10895219422793119	No Hit
GCATTCGGCAGGGAGAAGAGCATCCAGAAAGTCTGCCAAGGGC	406	0.10815303387907106	No Hit
GCAATCGAGGCATTACAGTCCGACGATCCTAAAGCATTCGATT	402	0.10708748674725754	No Hit
GCAGTCAAAGCTGCCGACGATCAGCTACGGGAAAGAGTCCTGA	398	0.10602193961544405	No Hit
GCATTCGAGGTCTCAGAGAAGCTGAAGCAGTCCCTACCCCCGG	398	0.10602193961544405	No Hit
GCATTCGACGCGGCGTTCTTCGACCGGGCCGTCGAGGAAGGCC	389	0.10362445856886364	No Hit
GCATTCGATTCTCCACAAAGGGGCCGTACCTCCCCAGTTTAAG	386	0.10282529822000352	No Hit
GCATTCGAACTTGGCTAGACCTGGATTTAATAGCATATCTCAT	377	0.10042781717342311	No Hit
GCATTCGACCAGTTCTGAGCGCATTCGATTTTTTTTTTTTTTT	376	0.10016143039046975	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTGCGG	70	0.0	37.181248	36
AACCGGG	20	0.0018300551	37.04231	8
ACGCCCT	20	0.0018300551	37.04231	8
AATTTCG	210	0.0	37.04231	8
ATAGCGG	30	8.2322495E-6	37.04231	8
AGGCGGG	30	8.2322495E-6	37.04231	8
AAACGGG	30	8.2322495E-6	37.04231	8
ACGATAC	40	3.783316E-8	37.04231	8
GAGAACG	85	0.0	37.04231	7
ACTACGG	20	0.0018300551	37.04231	8
GAACGTA	20	0.0018300551	37.04231	9
CAAGCGG	30	8.2322495E-6	37.04231	9
ACGTCCA	20	0.0018300551	37.04231	8
GTTCAAC	95	0.0	37.04231	7
ATGCGGC	30	8.2322495E-6	37.04231	8
GATTGCG	55	1.2732926E-11	37.04231	7
TGAGCGG	20	0.0018300551	37.04231	9
GCGTAAC	30	8.2322495E-6	37.04231	9
GATAGTG	40	3.783316E-8	37.04231	7
AATCGCC	20	0.0018300551	37.04231	8

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content