##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099150_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 375394 Sequences flagged as poor quality 0 Sequence length 43 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.530418706745444 37.0 35.0 37.0 30.0 37.0 2 34.9677272412452 37.0 35.0 37.0 32.0 37.0 3 32.83002392153311 35.0 32.0 35.0 27.0 37.0 4 35.51960606722537 37.0 35.0 39.0 30.0 39.0 5 36.23345338497685 39.0 37.0 39.0 32.0 39.0 6 36.89533130524196 39.0 37.0 39.0 32.0 39.0 7 36.4289333340437 39.0 37.0 39.0 32.0 39.0 8 32.59532917414769 34.0 32.0 37.0 25.0 39.0 9 30.861750587382858 31.0 31.0 34.0 28.0 34.0 10 31.124027022275264 31.0 31.0 34.0 28.0 34.0 11 31.088086117519193 31.0 31.0 34.0 27.0 34.0 12 34.6204228091019 37.0 35.0 37.0 31.0 37.0 13 34.43190354667363 37.0 35.0 37.0 30.0 37.0 14 31.657317378540945 31.0 31.0 34.0 30.0 34.0 15 31.729215171260062 31.0 31.0 34.0 30.0 34.0 16 31.73569902555715 31.0 31.0 34.0 30.0 34.0 17 35.338732105467855 37.0 35.0 37.0 33.0 37.0 18 35.453115926200205 37.0 35.0 37.0 33.0 37.0 19 35.34526923712153 37.0 35.0 37.0 33.0 37.0 20 35.27654943872305 36.0 35.0 37.0 33.0 37.0 21 35.339291517712056 37.0 35.0 37.0 33.0 37.0 22 37.01732046862763 39.0 37.0 39.0 33.0 39.0 23 37.088184680628885 39.0 37.0 39.0 33.0 39.0 24 37.224763848116915 39.0 37.0 39.0 34.0 39.0 25 37.312780705072534 39.0 37.0 39.0 34.0 39.0 26 37.3535192357896 39.0 37.0 39.0 35.0 39.0 27 38.72655663116619 40.0 38.0 41.0 36.0 41.0 28 38.8362467167829 40.0 38.0 41.0 36.0 41.0 29 38.92439410326217 40.0 38.0 41.0 36.0 41.0 30 38.92766000522118 40.0 38.0 41.0 36.0 41.0 31 38.94188239556306 40.0 38.0 41.0 36.0 41.0 32 38.98136091679675 40.0 38.0 41.0 36.0 41.0 33 38.9803459831537 40.0 38.0 41.0 36.0 41.0 34 38.92049952849539 40.0 38.0 41.0 35.0 41.0 35 38.89849065248779 40.0 38.0 41.0 35.0 41.0 36 38.87839976131744 40.0 38.0 41.0 35.0 41.0 37 38.85721668433699 40.0 38.0 41.0 35.0 41.0 38 38.828686659882685 40.0 38.0 41.0 35.0 41.0 39 38.757476145063585 40.0 38.0 41.0 35.0 41.0 40 38.71288033372936 40.0 38.0 41.0 35.0 41.0 41 38.6556178308657 40.0 38.0 41.0 35.0 41.0 42 38.43733250931022 40.0 38.0 41.0 35.0 41.0 43 38.44670399633452 40.0 38.0 41.0 35.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5635602099321346 1101 2 0.6431269363615755 1101 3 0.7084734934085404 1101 4 0.932774657325453 1101 5 0.8786376599999244 1101 6 0.49235190479451774 1101 7 0.8743262870944335 1101 8 1.2330552503669239 1101 9 0.4610347213267971 1101 10 0.5319445054877008 1101 11 0.8573508501146456 1101 12 0.6177290870723482 1101 13 0.7164743094693975 1101 14 0.31748724635358627 1101 15 0.27290221995835395 1101 16 0.23037535373879336 1101 17 0.3743583305354292 1101 18 0.2080695102879062 1101 19 0.13847476093725675 1101 20 0.1563687661200035 1101 21 0.009965419736460035 1101 22 0.1357633770933404 1101 23 0.15534709314007955 1101 24 0.29872683530755495 1101 25 0.3293608444399112 1101 26 0.26205865531841965 1101 27 0.33076079084362675 1101 28 0.40050681767752394 1101 29 0.5699041117790458 1101 30 0.4081611857551337 1101 31 0.43765877563865274 1101 32 0.4959111527553475 1101 33 0.567741799759105 1101 34 0.5321236607665583 1101 35 0.5352687579129451 1101 36 0.35603238107486135 1101 37 0.35486502540069864 1101 38 0.4941663716891256 1101 39 0.4226987259191901 1101 40 0.4482843837946078 1101 41 0.4671133118882054 1101 42 0.841465617314249 1101 43 0.7046455722323017 1102 1 0.5987580833939461 1102 2 0.47871551538492696 1102 3 0.7540290744255174 1102 4 0.5807443633223812 1102 5 0.973214758466888 1102 6 0.5653370786069374 1102 7 0.8160756397377114 1102 8 0.9775383961768611 1102 9 0.5991508559769976 1102 10 0.6538079762363402 1102 11 0.8169340211313951 1102 12 0.6055954451465411 1102 13 0.6395706224004911 1102 14 0.3246883737854702 1102 15 0.30435917360073717 1102 16 0.24585462686604131 1102 17 0.23950189477842088 1102 18 0.12881700679332653 1102 19 0.19219964005914392 1102 20 0.28629268738309577 1102 21 0.27870439175689654 1102 22 0.03813281727299511 1102 23 0.16618602162148477 1102 24 0.21142533893962678 1102 25 0.14254973511110336 1102 26 0.313715430221599 1102 27 0.37395609779382966 1102 28 0.4304685491562452 1102 29 0.3572044623828674 1102 30 0.4583972131618097 1102 31 0.3899319716330609 1102 32 0.5254374938722393 1102 33 0.42647481380617336 1102 34 0.5301930482410668 1102 35 0.34781602068248674 1102 36 0.4529468383264046 1102 37 0.43567003926444414 1102 38 0.5234750176251026 1102 39 0.5405978528469362 1102 40 0.35426298092102826 1102 41 0.473455116237524 1102 42 0.7617318928934296 1102 43 0.6051588745536236 1103 1 0.5391554343873253 1103 2 0.5581857140604214 1103 3 0.8252211274056549 1103 4 0.6684927076932397 1103 5 0.5775193942284815 1103 6 0.46931058854070784 1103 7 0.4518372291416881 1103 8 0.9924390584285163 1103 9 0.3524621142551432 1103 10 0.4170530093489262 1103 11 0.6033578621910003 1103 12 0.7297676305770082 1103 13 0.6346037349832727 1103 14 0.23694002941460823 1103 15 0.3689287100245764 1103 16 0.2276427063362405 1103 17 0.22956811994398407 1103 18 0.11060508626352572 1103 19 0.22034533542338153 1103 20 0.021392025131440562 1103 21 -0.0756002440046899 1103 22 0.21197387687564628 1103 23 0.11155026003208235 1103 24 0.21639222635684519 1103 25 0.13261596027666656 1103 26 -0.04555609295720586 1103 27 0.12561172693290246 1103 28 0.1672235160436628 1103 29 0.15852896569412422 1103 30 0.33091376945320405 1103 31 0.44291210408339055 1103 32 0.5866957720179329 1103 33 0.2691900455942573 1103 34 0.28515993565828524 1103 35 0.25344515975533 1103 36 0.35360908998203655 1103 37 0.24361705913199216 1103 38 0.2834087924595394 1103 39 0.44788262105885934 1103 40 0.24995834515944182 1103 41 0.14729617584017518 1103 42 0.47530805183382796 1103 43 0.3915827156132252 1104 1 0.6008031165065262 1104 2 0.41455392598094676 1104 3 0.24258006780300434 1104 4 0.6969324427925798 1104 5 0.6210902551556288 1104 6 0.26145363489832363 1104 7 0.7150266331152011 1104 8 0.39184832995169927 1104 9 0.6321018493544805 1104 10 0.5959112874946193 1104 11 0.3522214383499396 1104 12 0.572901405411443 1104 13 0.611173271407111 1104 14 0.21899036054043464 1104 15 0.37210221995835724 1104 16 0.30627846792564384 1104 17 0.30627805371882033 1104 18 0.22648058295227003 1104 19 0.09897579899953968 1104 20 0.2587244754625644 1104 21 0.09685538513438274 1104 22 0.16557652588226546 1104 23 0.20006681632347068 1104 24 0.13070745814491858 1104 25 0.19396430464752257 1104 26 0.1664545030692821 1104 27 0.3310209984560828 1104 28 0.34472550279863157 1104 29 0.4487276411908141 1104 30 0.5057058224333346 1104 31 0.4198207133549161 1104 32 0.5193076925477342 1104 33 0.5208933568525325 1104 34 0.555520200558945 1104 35 0.3565974776361287 1104 36 0.4004143879952764 1104 37 0.38562765515847985 1104 38 0.36075183881715844 1104 39 0.48823228993303047 1104 40 0.45301794780844773 1104 41 0.5981306129262691 1104 42 0.8400206346152856 1104 43 0.6931853646198434 1105 1 0.6522507945251022 1105 2 0.5155545451759949 1105 3 0.2581912133138431 1105 4 0.4773931239071345 1105 5 0.7036240012856609 1105 6 0.6971415605949218 1105 7 0.4920644040130284 1105 8 0.5005839336668672 1105 9 0.43071237567027154 1105 10 0.5355868292902848 1105 11 0.5044096736440551 1105 12 0.47267354163435726 1105 13 0.5025788441625281 1105 14 0.10932844103579242 1105 15 0.18466692584070543 1105 16 0.2219069508977789 1105 17 0.21081799179931693 1105 18 0.03760875632688254 1105 19 0.0613485544174992 1105 20 0.07505388722726991 1105 21 0.013516685443981657 1105 22 0.015384575417868973 1105 23 0.10945381322749625 1105 24 0.00570188538950589 1105 25 0.06556554303762141 1105 26 0.09378329563894283 1105 27 0.17942223684617886 1105 28 0.15310197338686038 1105 29 0.21541494769235925 1105 30 0.41526371717017696 1105 31 0.3750108062341724 1105 32 0.4206971662319461 1105 33 0.11536518347791969 1105 34 0.2463833584536843 1105 35 0.28431264791476707 1105 36 0.41476485239156347 1105 37 0.3140709988117365 1105 38 0.4315184022846452 1105 39 0.4578471506141426 1105 40 0.29457831932547407 1105 41 0.3787869596754945 1105 42 0.6252936996307668 1105 43 0.5618664791709307 1106 1 0.23142986809888555 1106 2 0.3525080661083422 1106 3 0.11475586398135107 1106 4 0.45425218801550926 1106 5 0.5094698729900244 1106 6 0.17670713808303873 1106 7 0.41406357579035813 1106 8 0.3172903681682584 1106 9 -0.0528612079703592 1106 10 0.15187816647551244 1106 11 0.20101889057923827 1106 12 0.11868229713118694 1106 13 0.3472624433816307 1106 14 0.26907188920285563 1106 15 0.17417610530867123 1106 16 0.23450776728870082 1106 17 0.1489379263302837 1106 18 0.03539523263379607 1106 19 0.193992359509096 1106 20 0.12732065380651392 1106 21 -0.043689837795554354 1106 22 0.10278671696507047 1106 23 0.28183751696041526 1106 24 0.191273063240466 1106 25 0.035650928851346464 1106 26 0.18330036294189256 1106 27 0.3596987054624492 1106 28 0.19440957923175262 1106 29 0.0666257303627873 1106 30 0.14095932561804858 1106 31 0.04744873883262102 1106 32 0.28006692821652024 1106 33 0.257706095706034 1106 34 0.21118389482646194 1106 35 0.2649923820947251 1106 36 0.08905642621184029 1106 37 0.16674103095466108 1106 38 0.17551871779804884 1106 39 -0.0030390476465882443 1106 40 -0.006115810153339396 1106 41 0.0781815683402769 1106 42 0.4861276409847122 1106 43 0.38589874041602457 1107 1 0.3775310668170846 1107 2 0.4594135533101422 1107 3 0.46193907401418244 1107 4 0.44296722540127575 1107 5 0.5228219321742671 1107 6 0.40058655415298006 1107 7 0.8050415399474957 1107 8 0.6125390131815109 1107 9 0.1440173773047917 1107 10 0.39446035581759986 1107 11 0.2822686433188828 1107 12 0.29870761659157097 1107 13 0.5081794825872379 1107 14 0.2032412922174558 1107 15 0.12538669200804264 1107 16 0.24190579711818927 1107 17 0.29469296055111727 1107 18 0.12760356431872566 1107 19 0.11695592322314496 1107 20 0.010841245648904874 1107 21 0.06270134786730353 1107 22 0.13027217805618108 1107 23 0.07412892812471483 1107 24 0.0016540419958559482 1107 25 0.31353200651087576 1107 26 0.24720232915623797 1107 27 0.371488079201427 1107 28 0.3501242605626018 1107 29 0.15357236168770783 1107 30 0.2747766298867518 1107 31 0.20028530962820668 1107 32 0.1561499285725816 1107 33 0.12474925747365262 1107 34 0.16285933099372585 1107 35 0.1743862975119015 1107 36 0.28647898426856955 1107 37 0.21649970484792647 1107 38 -0.007364931369181704 1107 39 0.2548732837218495 1107 40 0.12362664346061791 1107 41 -0.006440815645163411 1107 42 0.34227156629230393 1107 43 0.22758288014779282 1108 1 0.25519560476944037 1108 2 0.12738272097312375 1108 3 0.3329137141253824 1108 4 0.11808799834813755 1108 5 0.23644424576595924 1108 6 -0.05627782050073904 1108 7 0.13596184438280545 1108 8 0.2601250122678138 1108 9 0.30283987752349617 1108 10 0.2913003328780306 1108 11 0.16997167309172312 1108 12 0.2706090736430582 1108 13 0.44408719941963426 1108 14 -0.10791793366926683 1108 15 0.01155636706320351 1108 16 0.0318872316189136 1108 17 0.03328149659832036 1108 18 -0.02913882236854448 1108 19 -0.0766185671976416 1108 20 -0.04816253549204674 1108 21 -0.17057998262774277 1108 22 -0.10518403749801308 1108 23 0.09987276311532867 1108 24 0.07045331104006891 1108 25 0.07560687115769582 1108 26 -0.02858524653947825 1108 27 -0.028846294188674904 1108 28 0.09455461077985206 1108 29 0.1788058884521604 1108 30 0.21939846719077138 1108 31 0.10219191836274177 1108 32 0.1706353920782533 1108 33 0.11403764480245826 1108 34 0.08478217238992869 1108 35 0.066356476071185 1108 36 0.14613207187634458 1108 37 0.24767710742765559 1108 38 0.0391192879564386 1108 39 0.28882822107544115 1108 40 0.20799760352049645 1108 41 0.27033092591531016 1108 42 0.3687492731129396 1108 43 0.09398661657602503 1109 1 0.43146936454420626 1109 2 0.1573181332023026 1109 3 0.15294992651571704 1109 4 0.45735190904061795 1109 5 0.23743185641151854 1109 6 0.19413306974918498 1109 7 0.4261424886881926 1109 8 0.42436073183552736 1109 9 0.04399007541413624 1109 10 0.2640255732089045 1109 11 0.27557968010818 1109 12 0.3908003065103429 1109 13 0.27060184283568134 1109 14 -0.11255626426333265 1109 15 -0.0897351426790074 1109 16 -0.06466030758456043 1109 17 0.11909061258852205 1109 18 0.011399892214434715 1109 19 -0.08990708954048898 1109 20 -0.008287141491905459 1109 21 -0.19521620042291943 1109 22 -0.23588030300313534 1109 23 0.12535410046789508 1109 24 -0.09984073651755665 1109 25 -0.09439236725200573 1109 26 -0.1784170824880178 1109 27 -0.1100490172425026 1109 28 -0.015704010545171343 1109 29 -0.04081709978250103 1109 30 -0.06296482120873748 1109 31 -0.05019247345827438 1109 32 0.03583390918823426 1109 33 -0.07271166669534779 1109 34 0.031230090668834976 1109 35 0.05029920448070868 1109 36 -0.05348074544271242 1109 37 -0.08508380481011812 1109 38 0.011763455771628628 1109 39 -0.047205072704336715 1109 40 -0.057726165221382075 1109 41 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0.02771149520742 2216 23 -0.795074287971012 2216 24 -0.2951870203826843 2216 25 -0.21215103277578606 2216 26 0.16524714110609295 2216 27 0.02747253219841639 2216 28 0.2794101653753174 2216 29 -0.061456407888940134 2216 30 0.1398358837830287 2216 31 0.11821580537945664 2216 32 0.12944756984834527 2216 33 0.1305031728034507 2216 34 -0.09814360312203974 2216 35 -0.16296080457246376 2216 36 -0.05325923163662338 2216 37 -0.08085578042433639 2216 38 0.031092943111637794 2216 39 -0.3164695505577697 2216 40 0.1508338987286919 2216 41 -0.4797098778712794 2216 42 0.15105867142509766 2216 43 0.08768843210450683 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 3.0 25 24.0 26 171.0 27 801.0 28 2061.0 29 3761.0 30 6238.0 31 9641.0 32 13471.0 33 16929.0 34 23337.0 35 38251.0 36 70976.0 37 130947.0 38 58783.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 95.63398929609433 2.21856959803078 0.3042274833034523 1.8432136225714415 2 0.880420044274073 1.575698808436049 0.7211186262783406 96.82276252101154 3 1.5898047907254285 94.86510101440628 1.0152054385815361 2.529888756286762 4 0.7557393032387305 2.425984432356404 95.21462783102554 1.6036484333793295 5 1.0985907989024748 1.1100455526252697 95.12773382349023 2.663629824982019 6 0.6782225560945462 1.1060408691691108 1.9203874339692002 96.29534914076714 7 93.43889353585833 4.68014938970788 0.6510492975380534 1.2299077768957416 8 1.712885265990037 93.05735368550042 2.250459522097019 2.979301526412531 9 30.746376135166493 21.93041646108096 23.109256024216883 24.213951379535665 10 29.157751980325962 20.47762291609493 24.3367606583235 26.02786444525561 11 26.564411129083403 21.735608323629183 26.300866844849963 25.399113702437454 12 30.11893436091768 22.517848148829177 26.51066358604553 20.852553904207607 13 32.750133918220605 20.05681863608623 25.460452150553397 21.732595295139767 14 33.510259874179646 23.56533625368708 20.709464770568374 22.214939101564894 15 28.868547315027694 24.63748811163475 24.429691475276197 22.064273098061363 16 24.39061426082509 23.663080947738013 31.829515848025192 20.116788943411706 17 23.410336872725722 25.32086288006734 30.411514302306376 20.857285944900557 18 20.012093992162878 27.18564277970021 29.00453657899854 23.79772664913837 19 19.931744796939437 29.02071633329426 24.572938756806874 26.47460011295943 20 23.165098511406864 24.793014161347728 28.342674779161825 23.699212548083583 21 23.551788254473966 27.142948475468444 26.04756602396415 23.257697246093436 22 22.206801387342363 26.156518218192087 27.349131845474357 24.287548548991193 23 22.117029041487076 26.744700234953143 27.561175724705244 23.577094998854538 24 22.08879204249402 27.67359094711157 26.4964277532406 23.741189257153817 25 22.058423949237334 25.204984629482624 29.543359776661323 23.19323164461872 26 23.406125868834902 24.926536995249883 28.154113795060198 23.51322334085502 27 25.082713096107025 26.608310202081014 27.63256738253675 20.676409319275216 28 23.029403773102395 26.407454567734167 29.046015652887363 21.517126006276072 29 22.30882752521351 27.57849086559721 27.716745605950017 22.395936003239264 30 23.536870594628574 25.106421519789873 28.836102867920104 22.520605017661445 31 22.54644453560792 25.80755153252316 29.203983015178718 22.442020916690197 32 21.888436026532407 26.5555821945177 26.590745624550465 24.965236154399424 33 22.70734215251176 26.412782303393236 27.159198069228594 23.720677474866406 34 21.04866379677777 28.797630210553233 28.94441010996505 21.209295882703945 35 21.785377496710122 26.858181004491282 29.737555741434335 21.61888575736426 36 22.659392531580153 26.79584649728019 30.187216631059634 20.357544340080022 37 20.708541227679937 29.223130016436194 29.10458695067277 20.9637418052111 38 21.13033239742777 29.990356798457086 28.46316137178538 20.416149432329767 39 21.714518612444525 29.37447055626888 27.59926903466758 21.311741796619017 40 22.810181546945135 28.60449938063588 29.679662213460844 18.905656858958135 41 20.135860516536354 28.104213007978473 28.201180121741682 23.55874635374349 42 21.952238726008865 26.915607462866483 28.71757095214497 22.41458285897968 43 21.645510609107983 28.756876273692345 30.791853696871215 18.805759420328464 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 5.0 4 10.0 5 10.0 6 54.5 7 99.0 8 206.5 9 314.0 10 561.0 11 808.0 12 808.0 13 1183.5 14 1559.0 15 1814.5 16 2070.0 17 2145.0 18 2220.0 19 2220.0 20 2358.5 21 2497.0 22 2943.5 23 3390.0 24 3811.0 25 4232.0 26 4232.0 27 5320.5 28 6409.0 29 7580.5 30 8752.0 31 10472.5 32 12193.0 33 12193.0 34 13632.0 35 15071.0 36 16256.0 37 17441.0 38 19596.0 39 21751.0 40 21751.0 41 24567.5 42 27384.0 43 28002.0 44 28620.0 45 29337.5 46 30055.0 47 30055.0 48 31165.0 49 32275.0 50 33522.0 51 34769.0 52 34922.5 53 35076.0 54 35076.0 55 29851.5 56 24627.0 57 21619.5 58 18612.0 59 15137.5 60 11663.0 61 11663.0 62 9936.5 63 8210.0 64 7842.0 65 7474.0 66 9320.5 67 11167.0 68 11167.0 69 7359.0 70 3551.0 71 2379.0 72 1207.0 73 1109.5 74 1012.0 75 1012.0 76 750.5 77 489.0 78 342.5 79 196.0 80 190.5 81 185.0 82 185.0 83 95.5 84 6.0 85 3.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004528575310207409 2 0.0013319339147668848 3 5.327735659067539E-4 4 0.0 5 0.0010655471318135078 6 2.6638678295337696E-4 7 0.0 8 0.0010655471318135078 9 0.11960766554606626 10 0.02051178228741003 11 0.03329834786917212 12 0.03862608352823966 13 0.0439538191873072 14 0.02583951794647757 15 0.0071924431397411785 16 0.004262188527254031 17 0.0 18 2.6638678295337696E-4 19 0.010122697752228325 20 0.0015983206977202619 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.009323537403368195 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0010655471318135078 33 0.0 34 5.327735659067539E-4 35 0.0 36 0.0 37 7.991603488601309E-4 38 0.0 39 0.0 40 0.002397481046580393 41 0.002397481046580393 42 0.5538181217600708 43 0.002397481046580393 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 375394.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.42595779367811 #Duplication Level Percentage of deduplicated Percentage of total 1 59.2989482695365 10.333409697544447 2 6.489238106885166 2.2616237872741705 3 3.5251314663079367 1.842863764471462 4 2.8142961966491375 1.961672269668668 5 2.4734010028127673 2.15506907409282 6 2.422954628836982 2.5333383058866152 7 2.299131710896417 2.804520050933153 8 2.299131710896417 3.205165772495032 9 2.014797603032897 3.1598800193929577 >10 15.46410664057723 44.753245922950285 >50 0.5289225877461171 6.463342514797786 >100 0.33478048183930537 11.118984320473956 >500 0.018344135991194815 2.0341294746319867 >1k 0.01681545799192858 5.3727550253866605 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCATTCGACGCGGGTTCTTCGACCGGGCCGTCGAGGAAGGCCT 4876 1.2989019536806663 No Hit GCATTCGAGCGGAGGAGAATTGCCTGCTGTCCTGCTGTAAACT 2521 0.6715610798254634 No Hit GCATTCGAGACAGGGTTACGGTACCGCGCGCCCTGATCGCCGA 2388 0.6361316376926642 No Hit GCATTCGAGCCCTTCGGGATGTTGCTGACGCCGCCGGATGTCT 1594 0.4246205320276829 No Hit GCATTCGAGACAGGGGTTACGGTACCGCGCGCCCTGATCGCCG 1590 0.4235549848958694 No Hit GCATTCGAGGTGAGCCTTCACAGTGAGCTATCCCAAAGCTCAG 1560 0.4155633814072681 No Hit GCATCCGCGTCCGGGTGCTTCACCCATTCGGGCATGCCCTGCA 1244 0.331385157994001 No Hit GCATTCGAGGAACCAAGAACACAACATAGCAGGAAGTATTCAA 1141 0.3039473193498031 No Hit GCATTCGAATCACCGTTTATTGCAGCTCTTACCATTTCCCTAG 1117 0.2975540365589221 No Hit GCATTCGACGCGGGGTTCTTCGACCGGGCCGTCGAGGAAGGCC 1088 0.2898288198532742 No Hit GCATTCGACCAGTAGTCCGACGATCTGAGCGCATTCGATTTTT 1050 0.27970612210104584 No Hit GCATTCGAATTTCGCAGAATCATCATATTCTAGGAATTATAGA 1000 0.266386782953377 No Hit GCATTCGATCTCTGTCTTAAGGAGCGCGGCGATGCTTGAAATG 808 0.2152405206263286 No Hit GCATTCGAACGCTGAGTTCTAGAGTCGGACGATCGTTTAGCAT 755 0.20112202112979963 No Hit GCACTCGAGGGGCAAGTGATCGACTCGAAGCAAACAACCAACC 682 0.1816757859742031 No Hit GCAACCGAGTACAGTCAACTGCTAGTTTTTTTTTTTTTTTTTT 615 0.16382787151632683 No Hit GCATCTGAAGTTGGACCTTTCTCAGCATGGCTTACATCTTAGC 587 0.1563690415936323 No Hit GCATTCGAATCACGCTATGGGAACTGTGTTACCAGAGGAAAAT 580 0.15450433411295864 No Hit GCATTCGAGCTTGACATAACTCCAACATCATCAACCTCATACA 544 0.1449144099266371 No Hit GCATTCGATGTGTGTTCTACAGTCCGACGATCCGCGAGCATTC 531 0.14145138174824318 Illumina DpnII expression Sequencing Primer (95% over 21bp) GCATTCGTTCAACAGCCACGTTTGGCGTATAGCGTTTCAGCAG 513 0.1366564196550824 No Hit GCATTCGAATAGTAGGTGGCTTTCTGAGTTCCAGTCCAGCCTG 511 0.13612364608917565 No Hit GCATTCAAGCGCGGCATTCGATTTTTTTTTTTTTTTTTTTTTT 510 0.13585725930622225 No Hit GCATTCGAACCAAGGCGAGAAAACTGAAAATCACGGAAAATGA 498 0.13266061791078174 No Hit GCATTCGACGGTACCAAAACTGAGTGATAGAGGCAGGAAGCTC 493 0.13132868399601486 No Hit GAATTCGAGCAGTGCTGACTGAGCGTCCACTTCTGTTAACATA 487 0.1297303632982946 No Hit GCATTCGAGCAAAGTCCGACGATCCGAGAGCATTCGATTTTTT 462 0.12307069372446017 No Hit GCATTCGACATTGTGCAGCTCCCAGGACAGAGGCCCCACTCTT 452 0.12040682589492639 No Hit GCATTCAATATTACTGTGGTGTTTCACTGCAGGGACCTCTAGT 438 0.11667741093357913 No Hit GCATTCGAACAGAGGCGAGGAAAACTGAAAAAGGTAGAAAATG 437 0.11641102415062574 No Hit GCATTCGACAATGGGCGTCTGAAGGCAAGATGGGTCACCAGCA 409 0.10895219422793119 No Hit GCATTCGGCAGGGAGAAGAGCATCCAGAAAGTCTGCCAAGGGC 406 0.10815303387907106 No Hit GCAATCGAGGCATTACAGTCCGACGATCCTAAAGCATTCGATT 402 0.10708748674725754 No Hit GCAGTCAAAGCTGCCGACGATCAGCTACGGGAAAGAGTCCTGA 398 0.10602193961544405 No Hit GCATTCGAGGTCTCAGAGAAGCTGAAGCAGTCCCTACCCCCGG 398 0.10602193961544405 No Hit GCATTCGACGCGGCGTTCTTCGACCGGGCCGTCGAGGAAGGCC 389 0.10362445856886364 No Hit GCATTCGATTCTCCACAAAGGGGCCGTACCTCCCCAGTTTAAG 386 0.10282529822000352 No Hit GCATTCGAACTTGGCTAGACCTGGATTTAATAGCATATCTCAT 377 0.10042781717342311 No Hit GCATTCGACCAGTTCTGAGCGCATTCGATTTTTTTTTTTTTTT 376 0.10016143039046975 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0018647074806736389 0.0 0.0 12 0.0 0.0 0.0018647074806736389 0.0 0.0 13 0.0 0.0 0.022376489768083666 0.0 0.0 14 0.0 0.0 0.04208911170663356 0.0 0.0 15 0.0 0.0 0.067129469304251 0.0 0.0 16 0.0 0.0 0.10202613787114338 0.0 0.0 17 0.0 0.0 0.16222955081860657 0.0 0.0 18 0.0 0.0 0.22030186950244277 0.0 0.0 19 0.0 0.0 0.337778440784882 0.0 0.0 20 0.0 0.0 0.4850903317580995 0.0 0.0 21 0.0 0.0 0.5610105648998119 0.0 0.0 22 0.0 0.0 0.6664997309493492 0.0 0.0 23 0.0 0.0 0.9320873535538661 0.0 0.0 24 0.0 0.0 1.1710362978630453 0.0 0.0 25 0.0 0.0 1.2482884649195245 0.0 0.0 26 0.0 0.0 1.3167498681385423 0.0 0.0 27 0.0 0.0 1.3958667426756954 0.0 0.0 28 0.0 0.0 1.5543668785329547 0.0 0.0 29 0.0 0.0 1.7331124098946706 0.0 0.0 30 0.0 0.0 1.9725941277697565 0.0 0.0 31 0.0 0.0 2.2475052877776416 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTGCGG 70 0.0 37.181248 36 AACCGGG 20 0.0018300551 37.04231 8 ACGCCCT 20 0.0018300551 37.04231 8 AATTTCG 210 0.0 37.04231 8 ATAGCGG 30 8.2322495E-6 37.04231 8 AGGCGGG 30 8.2322495E-6 37.04231 8 AAACGGG 30 8.2322495E-6 37.04231 8 ACGATAC 40 3.783316E-8 37.04231 8 GAGAACG 85 0.0 37.04231 7 ACTACGG 20 0.0018300551 37.04231 8 GAACGTA 20 0.0018300551 37.04231 9 CAAGCGG 30 8.2322495E-6 37.04231 9 ACGTCCA 20 0.0018300551 37.04231 8 GTTCAAC 95 0.0 37.04231 7 ATGCGGC 30 8.2322495E-6 37.04231 8 GATTGCG 55 1.2732926E-11 37.04231 7 TGAGCGG 20 0.0018300551 37.04231 9 GCGTAAC 30 8.2322495E-6 37.04231 9 GATAGTG 40 3.783316E-8 37.04231 7 AATCGCC 20 0.0018300551 37.04231 8 >>END_MODULE