FastQCFastQC Report
Thu 26 May 2016
SRR2099149_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099149_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87867
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTCGACGCGGGTTCTTCGACCGGGCCGTCGAGGAAGGCCT14181.6138026790490172No Hit
GCATTCGAGCGGAGGAGAATTGCCTGCTGTCCTGCTGTAAACT8450.9616807220002959No Hit
GCATTCGAGACAGGGTTACGGTACCGCGCGCCCTGATCGCCGA7240.8239725949446322No Hit
GCATCCGCGTCCGGGTGCTTCACCCATTCGGGCATGCCCTGCA4540.5166899973824075No Hit
GCATTCGAGACAGGGGTTACGGTACCGCGCGCCCTGATCGCCG4540.5166899973824075No Hit
GCATTCGAGCCCTTCGGGATGTTGCTGACGCCGCCGGATGTCT4170.4745809006794359No Hit
GCATTCGAGGTGAGCCTTCACAGTGAGCTATCCCAAAGCTCAG4100.4666143148167116No Hit
GCATTCGAATCACCGTTTATTGCAGCTCTTACCATTTCCCTAG3210.36532486599064495No Hit
GCATTCGAGGAACCAAGAACACAACATAGCAGGAAGTATTCAA3200.36418678229597No Hit
GCATTCGAATTTCGCAGAATCATCATATTCTAGGAATTATAGA3090.35166786165454605No Hit
GCATTCGACGCGGGGTTCTTCGACCGGGCCGTCGAGGAAGGCC2850.3243538529823483No Hit
GCATTCGACCAGTAGTCCGACGATCTGAGCGCATTCGATTTTT2290.2606211660805535No Hit
GCATCTGAAGTTGGACCTTTCTCAGCATGGCTTACATCTTAGC2000.22761673893498127No Hit
GCATTCGATCTCTGTCTTAAGGAGCGCGGCGATGCTTGAAATG1980.22534057154563147No Hit
GCATTCGAACGCTGAGTTCTAGAGTCGGACGATCGTTTAGCAT1910.21737398568290714No Hit
GCACTCGAGGGGCAAGTGATCGACTCGAAGCAAACAACCAACC1810.20599314873615804No Hit
GCATTCAAGCGCGGCATTCGATTTTTTTTTTTTTTTTTTTTTT1800.20485506504148315No Hit
GCAACCGAGTACAGTCAACTGCTAGTTTTTTTTTTTTTTTTTT1720.1957503954840839No Hit
GCATTCGATGTGTGTTCTACAGTCCGACGATCCGCGAGCATTC1610.18323147484265995Illumina DpnII expression Sequencing Primer (95% over 21bp)
GCATTCGTTCAACAGCCACGTTTGGCGTATAGCGTTTCAGCAG1390.158193633559812No Hit
GCAATCGAGGCATTACAGTCCGACGATCCTAAAGCATTCGATT1350.15364129878111238No Hit
GAATTCGAGCAGTGCTGACTGAGCGTCCACTTCTGTTAACATA1350.15364129878111238No Hit
GCATTCAATATTACTGTGGTGTTTCACTGCAGGGACCTCTAGT1340.15250321508643747No Hit
GCATTCGAATAGTAGGTGGCTTTCTGAGTTCCAGTCCAGCCTG1320.15022704769708764No Hit
GCATTCGAACTTGGCTAGACCTGGATTTAATAGCATATCTCAT1310.14908896400241275No Hit
GCATTCGGCAGGGAGAAGAGCATCCAGAAAGTCTGCCAAGGGC1290.14681279661306293No Hit
GCATTCGAATCACGCTATGGGAACTGTGTTACCAGAGGAAAAT1230.1399842944450135No Hit
GCATTCGAACAGAGGCGAGGAAAACTGAAAAAGGTAGAAAATG1230.1399842944450135No Hit
GCATCCAAGCATGGCAGCTTCCATACCTGGATCCAGAGCCCAA1210.13770812705566368No Hit
GCATTCGAGCTTGACATAACTCCAACATCATCAACCTCATACA1200.13657004336098877No Hit
GCATTCGACGCGGCGTTCTTCGACCGGGCCGTCGAGGAAGGCC1160.13201770858228914No Hit
GCATTCGACGGTACCAAAACTGAGTGATAGAGGCAGGAAGCTC1140.12974154119293932No Hit
GCAGTCAAAGCTGCCGACGATCAGCTACGGGAAAGAGTCCTGA1040.11836070424619026No Hit
GCATTTGATTGAGAGGACGTGGAATATGGCAAGAAAACTGAAA990.11267028577281574No Hit
GCATTCGAGACACTGGACAGGCCACTGCAACGTTACCCCAGCT980.11153220207814082No Hit
GCATTCAAGGCGTCCCAAGACTTCTAGCTCTTTTCCAGTAAAT940.1069798672994412No Hit
GCATTCGAACCAAGGCGAGAAAACTGAAAATCACGGAAAATGA920.10470369991009139No Hit
GCATTCAACGGCGTGGAATCTACCTCTTTATAATATGCATCTA890.10128944882606668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGAGCT308.198802E-637.01708216
GGTGGCC200.001828544337.01708213
GGAGTTC551.2732926E-1137.01708213
GTCAACT251.2210409E-437.01708214
GTTCCTG200.001828544337.01708215
CCGTTTA200.001828544337.01708213
CTTCGGG308.230934E-636.99601712
GAAATCG308.230934E-636.9960171
GATACTC308.230934E-636.9960177
GATACAT251.2251442E-436.9960177
CCGGGTG308.230934E-636.99601711
CGAACTG551.2732926E-1136.9960176
TCGCAAG251.2251442E-436.99601712
GCAAGCG308.230934E-636.9960171
CGAACAA251.2251442E-436.9960176
ATTAGAC251.2251442E-436.9960173
TTGGACC251.2251442E-436.99601711
CGACGTT750.036.9960176
CGCGGGG251.2251442E-436.9960179
GAGTCTT251.2251442E-436.9960177
Produced by FastQC (version 0.11.5)