##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099149_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 87867 Sequences flagged as poor quality 0 Sequence length 43 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.87328576143489 37.0 35.0 37.0 31.0 37.0 2 35.57294547441019 37.0 36.0 37.0 32.0 37.0 3 34.72931817405852 35.0 35.0 37.0 32.0 37.0 4 36.51770289187067 39.0 37.0 39.0 32.0 39.0 5 36.73571420442259 39.0 37.0 39.0 32.0 39.0 6 37.472236448268404 39.0 39.0 39.0 34.0 39.0 7 36.891039867071825 39.0 38.0 39.0 32.0 39.0 8 34.29253303287924 35.0 33.0 37.0 30.0 39.0 9 31.3759773293728 33.0 31.0 34.0 28.0 34.0 10 31.561006976453047 34.0 31.0 34.0 28.0 34.0 11 31.577463666678046 34.0 31.0 34.0 28.0 34.0 12 34.88151410654739 37.0 35.0 37.0 32.0 37.0 13 34.73509963922747 37.0 35.0 37.0 31.0 37.0 14 32.07725312119453 31.0 31.0 34.0 30.0 34.0 15 32.202783752717174 33.0 31.0 34.0 30.0 34.0 16 32.040493017856534 33.0 31.0 34.0 30.0 34.0 17 35.13232499117985 37.0 35.0 37.0 32.0 37.0 18 35.49426974859731 37.0 35.0 37.0 33.0 37.0 19 35.44648161425791 37.0 35.0 37.0 33.0 37.0 20 35.28148224020394 37.0 35.0 37.0 32.0 37.0 21 35.45345806730627 37.0 35.0 37.0 33.0 37.0 22 37.27073873012621 39.0 37.0 39.0 33.0 39.0 23 37.29123561746731 39.0 37.0 39.0 33.0 39.0 24 37.53553666336622 39.0 37.0 39.0 35.0 39.0 25 37.60093095246224 39.0 37.0 39.0 35.0 39.0 26 37.70926513935835 39.0 37.0 39.0 35.0 39.0 27 39.236072700786416 40.0 39.0 41.0 36.0 41.0 28 39.35485449599964 40.0 39.0 41.0 36.0 41.0 29 39.448052169756565 40.0 39.0 41.0 37.0 41.0 30 39.33158068444354 40.0 39.0 41.0 37.0 41.0 31 39.279058121934284 40.0 39.0 41.0 36.0 41.0 32 39.297643028668325 40.0 39.0 41.0 36.0 41.0 33 39.31140246053695 40.0 39.0 41.0 36.0 41.0 34 39.285317582255 40.0 39.0 41.0 35.0 41.0 35 39.27004449907246 40.0 39.0 41.0 35.0 41.0 36 39.277032332957766 40.0 39.0 41.0 35.0 41.0 37 39.26088292533033 40.0 39.0 41.0 35.0 41.0 38 39.173352908372884 40.0 39.0 41.0 35.0 41.0 39 39.11127044282836 40.0 39.0 41.0 35.0 41.0 40 39.07078880580878 40.0 39.0 41.0 35.0 41.0 41 39.02418427851184 40.0 39.0 41.0 35.0 41.0 42 38.99311459364721 40.0 39.0 41.0 35.0 41.0 43 38.91542900064871 40.0 39.0 41.0 35.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5474716963840294 1101 2 0.1963302039744761 1101 3 0.6886228087648405 1101 4 0.7701352217008335 1101 5 0.33968046254273787 1101 6 0.38242645963401856 1101 7 0.6136817510979071 1101 8 0.9496347954333828 1101 9 0.5476719269291408 1101 10 0.5458933261450518 1101 11 0.6438445682021765 1101 12 0.34854563843355635 1101 13 0.27114723294499754 1101 14 -0.02408815603055814 1101 15 -0.058622718753937875 1101 16 -0.20715250435464228 1101 17 -0.1434028073413245 1101 18 -0.04302794491496087 1101 19 -0.9909554965219911 1101 20 -0.36885683518904955 1101 21 -0.06043278791787543 1101 22 -0.7036340395557161 1101 23 -0.7449561563784073 1101 24 -0.2231234334348713 1101 25 0.20134613599688578 1101 26 0.03525694586368644 1101 27 -0.10827116726547814 1101 28 0.23806752926342512 1101 29 -0.025225364459153354 1101 30 0.23484157429238195 1101 31 0.08912555955939183 1101 32 -0.09428772740292146 1101 33 0.2959830867623765 1101 34 -0.08131464671094335 1101 35 0.04394613169461792 1101 36 0.1484155548676469 1101 37 0.2621189825937549 1101 38 0.2557340212950834 1101 39 0.37564047143970214 1101 40 0.2041134736405752 1101 41 0.25237114538312966 1101 42 0.28092030238821053 1101 43 0.5445792042240356 1102 1 1.2080288811347586 1102 2 0.6914426184417337 1102 3 0.40978605412163205 1102 4 0.8749739313782499 1102 5 1.1410001106365755 1102 6 0.4919083755675473 1102 7 1.1745321909806066 1102 8 0.9332613838986745 1102 9 0.9528527675938534 1102 10 0.9786890250697802 1102 11 0.9495630432754893 1102 12 1.1513315621872167 1102 13 0.9588305174024754 1102 14 -0.1259943143883291 1102 15 -0.014878828235858066 1102 16 -0.1509452707280552 1102 17 -0.2726794446824812 1102 18 -0.0945919722854427 1102 19 -0.4496553987702825 1102 20 -0.5846437364598245 1102 21 0.013614132903235543 1102 22 -0.05309640514711589 1102 23 0.19810347216509427 1102 24 0.4112851687156649 1102 25 0.219674581744691 1102 26 0.382275518688715 1102 27 0.3066848444647263 1102 28 0.13078502681963045 1102 29 0.2726729737617646 1102 30 0.44012016666775367 1102 31 0.14337775897288196 1102 32 0.2576184309548495 1102 33 0.22340244160108824 1102 34 0.26203823696452133 1102 35 0.038081029055327065 1102 36 0.08365504655874645 1102 37 0.16778858181174172 1102 38 0.35764017965285433 1102 39 0.4958750755452712 1102 40 0.45875667989668756 1102 41 0.515078867768274 1102 42 0.609854808742071 1102 43 0.3864658122396776 1103 1 0.31385274477112546 1103 2 -0.0561899573158442 1103 3 0.6034413571519366 1103 4 0.41403643137824986 1103 5 0.4833296560911222 1103 6 0.6341905725372428 1103 7 0.5005265091624267 1103 8 0.8200985051107992 1103 9 0.43628079789688456 1103 10 0.4370223584031159 1103 11 0.4538244069118562 1103 12 0.4929508803690368 1103 13 0.4772964264933819 1103 14 0.14602273106621766 1103 15 0.10065147479444647 1103 16 0.1733918504840659 1103 17 0.02040767652964348 1103 18 -0.05789689652786478 1103 19 0.057683616381233094 1103 20 -0.18715320615679332 1103 21 -0.08084609436949108 1103 22 0.014611928186219814 1103 23 0.2842777145893365 1103 24 -0.1303814979509994 1103 25 0.08189049083559752 1103 26 0.18293839747659035 1103 27 0.1994405262829062 1103 28 -0.1414687610591585 1103 29 0.01509721618600679 1103 30 -0.026499151514066455 1103 31 0.10928684988197546 1103 32 0.12622638550030274 1103 33 -0.26878505839891176 1103 34 0.0683829339342168 1103 35 -0.06064056185376643 1103 36 -0.013882832229128894 1103 37 -0.2865201303094693 1103 38 0.14031063419830758 1103 39 -0.23423856081836192 1103 40 -0.15677362313361698 1103 41 0.040173564737969514 1103 42 -0.01041034277307773 1103 43 0.08414573648209966 1104 1 1.0716652447711255 1104 2 0.6059951202035379 1104 3 0.32909542691938043 1104 4 0.6928033887425897 1104 5 0.34924535376553933 1104 6 0.1985679950178607 1104 7 0.6516287378445966 1104 8 0.4459463733278568 1104 9 0.831993007199209 1104 10 0.8701618932868378 1104 11 1.049633515438991 1104 12 1.0857924501364806 1104 13 0.8698594109895055 1104 14 0.0357393008336615 1104 15 -0.14983302132958443 1104 16 0.2415846799414325 1104 17 0.11209856800250861 1104 18 -0.014958427535617602 1104 19 -0.30272093788233434 1104 20 -0.1415500084823762 1104 21 -0.6962167339043717 1104 22 -0.2585227617362591 1104 23 -0.21644902959670986 1104 24 0.2465564865451242 1104 25 0.23147866913016912 1104 26 -0.1420373777172088 1104 27 0.03307666969375589 1104 28 0.0502705800261154 1104 29 -0.09730588458918987 1104 30 0.02503912367973271 1104 31 0.09087599716879424 1104 32 -0.09403766488729559 1104 33 -0.121558798708989 1104 34 -0.042930050561906796 1104 35 -0.12453348821035348 1104 36 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1105 29 -0.29231019122140367 1105 30 -0.14102461447703263 1105 31 -0.12497240937728549 1105 32 -0.12487315153673251 1105 33 0.03451355271219825 1105 34 0.10160052652680918 1105 35 0.19346823444252692 1105 36 -0.07291061000690746 1105 37 0.15751922154237974 1105 38 0.3899634119760833 1105 39 0.4891410688112643 1105 40 0.08350415464416017 1105 41 0.058344861034264284 1105 42 0.025006323893592253 1105 43 0.45330082907469205 1106 1 0.13882942387560604 1106 2 0.18658056507221232 1106 3 0.2053536705847705 1106 4 0.27049631943795305 1106 5 0.5894397307179844 1106 6 0.31947508746261377 1106 7 0.8285349046848154 1106 8 0.18133917675258715 1106 9 0.4458423650610648 1106 10 0.453813403179236 1106 11 0.5221546307924498 1106 12 0.86503576842874 1106 13 0.5806079936575586 1106 14 0.053788589275171717 1106 15 0.10904699718250299 1106 16 -0.41033016444130865 1106 17 -0.4275867264554307 1106 18 0.025941722875117534 1106 19 0.14758566862003875 1106 20 0.22551004011186393 1106 21 0.19305782354095413 1106 22 0.09366976400711735 1106 23 -0.0408155689927554 1106 24 0.14387223339228683 1106 25 0.057869968447541 1106 26 0.11927235270047021 1106 27 0.16387616061126664 1106 28 -0.3623176416561762 1106 29 0.08226139529048737 1106 30 -0.10194224852899225 1106 31 -0.041832553103098746 1106 32 0.04052209445552535 1106 33 0.006984531153328533 1106 34 0.23885868020287404 1106 35 0.3389629829223537 1106 36 0.2048671677708711 1106 37 0.10118975775023387 1106 38 0.10323041031771396 1106 39 0.2923472600771575 1106 40 -0.012184070894811327 1106 41 0.27548140055886705 1106 42 0.24192174677916256 1106 43 -0.08108280829402048 1107 1 0.622389882452282 1107 2 0.3004676513798046 1107 3 0.125859835412804 1107 4 0.4256985690594064 1107 5 0.8386285691345989 1107 6 0.34467517398651637 1107 7 0.623148791771122 1107 8 0.25012567902384575 1107 9 0.5990978268823923 1107 10 0.5801383004321004 1107 11 0.5885617257524345 1107 12 0.7158901919632399 1107 13 0.5889359192470067 1107 14 -0.254452812412044 1107 15 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1108 8 -0.2233042726669794 1108 9 0.846118760859845 1108 10 0.6083186546994135 1108 11 0.8465327402451877 1108 12 0.665809676665333 1108 13 1.0068103153822676 1108 14 -0.09535458374859829 1108 15 0.025188511831480298 1108 16 0.05174833196554829 1108 17 0.011495639492608234 1108 18 0.05894338124991094 1108 19 0.02475537564049546 1108 20 -0.5487851506012404 1108 21 -0.30810303881393253 1108 22 -0.37531862736933874 1108 23 -0.18354635948473685 1108 24 -0.29797409054359036 1108 25 -0.5101233980532882 1108 26 -0.38338104696785535 1108 27 -0.5652006774207976 1108 28 0.04475809079269055 1108 29 -0.32193982085102846 1108 30 -0.7336172070696207 1108 31 -0.16200944641432358 1108 32 0.08994166327808273 1108 33 -0.23215311395446747 1108 34 -0.18728836236208224 1108 35 0.08235712333141976 1108 36 0.008570871474574915 1108 37 0.09825996228312306 1108 38 0.12329674530941759 1108 39 0.18543736510756048 1108 40 0.061281932421941576 1108 41 0.11019671288611477 1108 42 -0.07128997240270962 1108 43 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-0.24730824214256586 2216 32 -0.27556617584939147 2216 33 -0.4667816074786657 2216 34 -0.19896752618848268 2216 35 -0.28442315387830774 2216 36 -0.36261749480581784 2216 37 -0.37408723153646406 2216 38 -0.23689412040292268 2216 39 -0.49018181235210534 2216 40 -0.21697300963668198 2216 41 -0.19746063771601996 2216 42 -0.25638426915344326 2216 43 -0.28898501505164376 >>END_MODULE >>Per sequence quality scores pass #Quality Count 25 2.0 26 11.0 27 61.0 28 175.0 29 385.0 30 921.0 31 1906.0 32 2942.0 33 3458.0 34 4127.0 35 7383.0 36 14765.0 37 23938.0 38 27793.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 93.43325708172578 2.8326903160458423 0.25948308238587864 3.4745695198424893 2 0.7067580178908792 1.5762638563266793 0.787562879839756 96.92941524594268 3 1.6092503442703177 93.42642857955774 0.8842910307624022 4.080030045409539 4 0.6851263841942936 2.8873183333902377 94.76936733927414 1.6581879431413384 5 0.7568256569588128 1.229130390248899 94.71815357301377 3.2958903797785286 6 0.5724626135251406 0.9639678601506839 2.1521407597933218 96.31142876653085 7 92.8118633844333 5.306884268269089 0.5508325082226547 1.3304198390749655 8 1.6081671674405902 93.33970681963034 1.8949740508057908 3.1571519621232813 9 31.27027303870799 22.174294071452145 22.5783321762289 23.977100713610962 10 28.173582500625955 21.998270093096306 23.740696059909407 26.087451346368333 11 26.28302165970475 21.71319955838332 25.935874526229526 26.0679042556824 12 30.282026768642446 23.64335791678048 26.110807611763637 19.963807702813437 13 33.907313409054694 20.585892153961716 24.549882776045344 20.956911660938246 14 36.334763850786025 22.564230992521086 20.108825571732673 20.992179584960216 15 28.908641862900197 25.873234467295674 26.302311553213524 18.9158121165906 16 29.161971750833704 22.98630791818896 30.66775930162416 17.183961029353185 17 25.56022169870372 23.696040606826227 33.73735304494293 17.006384649527124 18 18.83549951061844 25.357931395534113 28.848473812396147 26.9580952814513 19 17.506516567447896 28.309790217750102 24.73336141053806 29.45033180426394 20 23.625954632886035 23.318651051092065 31.044490729675285 22.01090358634662 21 22.72070288048983 27.85573651086301 27.79997040982394 21.623590198823223 22 21.10120978296744 25.209691920743854 28.40770710278034 25.28139119350837 23 23.046194817166853 25.633059055162917 29.538962295287195 21.781783832383034 24 21.276474671947376 28.54200097875198 27.45626913403212 22.72525521526853 25 20.931635312460877 23.147484265992922 33.39251368545643 22.528366736089772 26 22.649003607725312 22.831096998873296 30.0465476231122 24.473351770289188 27 25.35195238257821 25.289357779371098 29.714227184267134 19.64446265378356 28 23.394448427737377 25.35536663366224 30.87279638544619 20.377388553154198 29 22.12207085709083 26.031388348299135 29.851935311322798 21.99460548328724 30 23.477528537448645 23.983975781578977 31.71384023581094 20.82465544516144 31 23.15203660077162 24.391409744272593 31.31892519375875 21.137628461197036 32 22.834412665171914 24.86257013760058 28.536471552303016 23.766545644924484 33 21.65318037488477 24.570088884336556 29.299964719405462 24.476766021373212 34 20.88544926876458 28.392420190064303 30.14397086439424 20.578159676776874 35 21.464258481568734 25.304152867401868 33.19448712258299 20.037101528446403 36 22.183527376603276 25.58981187476527 33.78970489489797 18.436955853733483 37 20.298415733406173 27.35932805244469 31.306337066375306 21.035919147773832 38 21.877382862735725 27.764689815289017 30.415286740186875 19.942640581788385 39 22.05492391910501 28.787827056801756 29.32500256068831 19.832246463404918 40 22.273441234719662 27.26672585308111 32.2952946800519 18.16453823214733 41 19.459810148193675 26.9662409797628 29.81743267545357 23.756516196589953 42 22.225636473306245 25.168720907735555 29.87697315260564 22.728669466352557 43 21.161419563386374 26.429010448678547 34.01966810079902 18.38990188713606 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 4.0 4 8.0 5 8.0 6 46.0 7 84.0 8 177.0 9 270.0 10 458.0 11 646.0 12 646.0 13 861.5 14 1077.0 15 1146.5 16 1216.0 17 1077.5 18 939.0 19 939.0 20 862.5 21 786.0 22 824.5 23 863.0 24 912.5 25 962.0 26 962.0 27 1193.5 28 1425.0 29 1659.5 30 1894.0 31 2287.5 32 2681.0 33 2681.0 34 2917.0 35 3153.0 36 3424.5 37 3696.0 38 4135.5 39 4575.0 40 4575.0 41 5047.5 42 5520.0 43 5759.5 44 5999.0 45 6228.0 46 6457.0 47 6457.0 48 6687.5 49 6918.0 50 7455.0 51 7992.0 52 8383.0 53 8774.0 54 8774.0 55 7355.5 56 5937.0 57 5208.5 58 4480.0 59 3595.5 60 2711.0 61 2711.0 62 2347.5 63 1984.0 64 1945.5 65 1907.0 66 2560.0 67 3213.0 68 3213.0 69 2081.0 70 949.0 71 607.0 72 265.0 73 267.5 74 270.0 75 270.0 76 195.5 77 121.0 78 87.0 79 53.0 80 47.5 81 42.0 82 42.0 83 21.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0011380836946749064 3 0.0 4 0.0 5 0.0 6 0.0011380836946749064 7 0.0 8 0.003414251084024719 9 0.0045523347786996255 10 0.0011380836946749064 11 0.009104669557399251 12 0.003414251084024719 13 0.0011380836946749064 14 0.022761673893498126 15 0.0045523347786996255 16 0.006828502168049438 17 0.0 18 0.0011380836946749064 19 0.01593317172544869 20 0.006828502168049438 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0045523347786996255 33 0.0 34 0.0022761673893498128 35 0.0 36 0.0 37 0.003414251084024719 38 0.0 39 0.0 40 0.010242753252074157 41 0.010242753252074157 42 0.0 43 0.010242753252074157 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 87867.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.33865956502441 #Duplication Level Percentage of deduplicated Percentage of total 1 49.53256526630203 14.532190697303879 2 16.175957174444317 9.491618013588718 3 10.54734473796501 9.283348697463211 4 6.912603281740952 8.112260575642733 5 4.829512393808915 7.084570999351292 6 3.305015710462004 5.817883847178122 7 1.9938709802552463 4.094825133440313 8 1.4158811435664689 3.3232043884507267 9 0.9154738352922922 2.417289767489501 >10 3.9877419605104927 20.26813251846541 >50 0.2560223437681834 5.075853278250082 >100 0.11637379262190155 7.099366087382066 >500 0.007758252841460103 1.7856533169449282 >1k 0.0038791264207300516 1.6138026790490172 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCATTCGACGCGGGTTCTTCGACCGGGCCGTCGAGGAAGGCCT 1418 1.6138026790490172 No Hit GCATTCGAGCGGAGGAGAATTGCCTGCTGTCCTGCTGTAAACT 845 0.9616807220002959 No Hit GCATTCGAGACAGGGTTACGGTACCGCGCGCCCTGATCGCCGA 724 0.8239725949446322 No Hit GCATCCGCGTCCGGGTGCTTCACCCATTCGGGCATGCCCTGCA 454 0.5166899973824075 No Hit GCATTCGAGACAGGGGTTACGGTACCGCGCGCCCTGATCGCCG 454 0.5166899973824075 No Hit GCATTCGAGCCCTTCGGGATGTTGCTGACGCCGCCGGATGTCT 417 0.4745809006794359 No Hit GCATTCGAGGTGAGCCTTCACAGTGAGCTATCCCAAAGCTCAG 410 0.4666143148167116 No Hit GCATTCGAATCACCGTTTATTGCAGCTCTTACCATTTCCCTAG 321 0.36532486599064495 No Hit GCATTCGAGGAACCAAGAACACAACATAGCAGGAAGTATTCAA 320 0.36418678229597 No Hit GCATTCGAATTTCGCAGAATCATCATATTCTAGGAATTATAGA 309 0.35166786165454605 No Hit GCATTCGACGCGGGGTTCTTCGACCGGGCCGTCGAGGAAGGCC 285 0.3243538529823483 No Hit GCATTCGACCAGTAGTCCGACGATCTGAGCGCATTCGATTTTT 229 0.2606211660805535 No Hit GCATCTGAAGTTGGACCTTTCTCAGCATGGCTTACATCTTAGC 200 0.22761673893498127 No Hit GCATTCGATCTCTGTCTTAAGGAGCGCGGCGATGCTTGAAATG 198 0.22534057154563147 No Hit GCATTCGAACGCTGAGTTCTAGAGTCGGACGATCGTTTAGCAT 191 0.21737398568290714 No Hit GCACTCGAGGGGCAAGTGATCGACTCGAAGCAAACAACCAACC 181 0.20599314873615804 No Hit GCATTCAAGCGCGGCATTCGATTTTTTTTTTTTTTTTTTTTTT 180 0.20485506504148315 No Hit GCAACCGAGTACAGTCAACTGCTAGTTTTTTTTTTTTTTTTTT 172 0.1957503954840839 No Hit GCATTCGATGTGTGTTCTACAGTCCGACGATCCGCGAGCATTC 161 0.18323147484265995 Illumina DpnII expression Sequencing Primer (95% over 21bp) GCATTCGTTCAACAGCCACGTTTGGCGTATAGCGTTTCAGCAG 139 0.158193633559812 No Hit GCAATCGAGGCATTACAGTCCGACGATCCTAAAGCATTCGATT 135 0.15364129878111238 No Hit GAATTCGAGCAGTGCTGACTGAGCGTCCACTTCTGTTAACATA 135 0.15364129878111238 No Hit GCATTCAATATTACTGTGGTGTTTCACTGCAGGGACCTCTAGT 134 0.15250321508643747 No Hit GCATTCGAATAGTAGGTGGCTTTCTGAGTTCCAGTCCAGCCTG 132 0.15022704769708764 No Hit GCATTCGAACTTGGCTAGACCTGGATTTAATAGCATATCTCAT 131 0.14908896400241275 No Hit GCATTCGGCAGGGAGAAGAGCATCCAGAAAGTCTGCCAAGGGC 129 0.14681279661306293 No Hit GCATTCGAATCACGCTATGGGAACTGTGTTACCAGAGGAAAAT 123 0.1399842944450135 No Hit GCATTCGAACAGAGGCGAGGAAAACTGAAAAAGGTAGAAAATG 123 0.1399842944450135 No Hit GCATCCAAGCATGGCAGCTTCCATACCTGGATCCAGAGCCCAA 121 0.13770812705566368 No Hit GCATTCGAGCTTGACATAACTCCAACATCATCAACCTCATACA 120 0.13657004336098877 No Hit GCATTCGACGCGGCGTTCTTCGACCGGGCCGTCGAGGAAGGCC 116 0.13201770858228914 No Hit GCATTCGACGGTACCAAAACTGAGTGATAGAGGCAGGAAGCTC 114 0.12974154119293932 No Hit GCAGTCAAAGCTGCCGACGATCAGCTACGGGAAAGAGTCCTGA 104 0.11836070424619026 No Hit GCATTTGATTGAGAGGACGTGGAATATGGCAAGAAAACTGAAA 99 0.11267028577281574 No Hit GCATTCGAGACACTGGACAGGCCACTGCAACGTTACCCCAGCT 98 0.11153220207814082 No Hit GCATTCAAGGCGTCCCAAGACTTCTAGCTCTTTTCCAGTAAAT 94 0.1069798672994412 No Hit GCATTCGAACCAAGGCGAGAAAACTGAAAATCACGGAAAATGA 92 0.10470369991009139 No Hit GCATTCAACGGCGTGGAATCTACCTCTTTATAATATGCATCTA 89 0.10128944882606668 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.02503784128284794 0.0 0.0 14 0.0 0.0 0.029590176061547565 0.0 0.0 15 0.0 0.0 0.042109096702971534 0.0 0.0 16 0.0 0.0 0.05918035212309513 0.0 0.0 17 0.0 0.0 0.1263272901089146 0.0 0.0 18 0.0 0.0 0.15364129878111235 0.0 0.0 19 0.0 0.0 0.16502213572786142 0.0 0.0 20 0.0 0.0 0.1786791400639603 0.0 0.0 21 0.0 0.0 0.2116835672095326 0.0 0.0 22 0.0 0.0 0.3004540953941753 0.0 0.0 23 0.0 0.0 0.7340639830653146 0.0 0.0 24 0.0 0.0 0.9616807220002959 0.0 0.0 25 0.0 0.0 1.0003755676192427 0.0 0.0 26 0.0 0.0 1.0390704132381896 0.0 0.0 27 0.0 0.0 1.1119077696973836 0.0 0.0 28 0.0 0.0 1.3031058304027678 0.0 0.0 29 0.0 0.0 1.471542217214654 0.0 0.0 30 0.0 0.0 1.7196444626537835 0.0 0.0 31 0.0 0.0 2.018960474353284 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGAGCT 30 8.198802E-6 37.017082 16 GGTGGCC 20 0.0018285443 37.017082 13 GGAGTTC 55 1.2732926E-11 37.017082 13 GTCAACT 25 1.2210409E-4 37.017082 14 GTTCCTG 20 0.0018285443 37.017082 15 CCGTTTA 20 0.0018285443 37.017082 13 CTTCGGG 30 8.230934E-6 36.996017 12 GAAATCG 30 8.230934E-6 36.996017 1 GATACTC 30 8.230934E-6 36.996017 7 GATACAT 25 1.2251442E-4 36.996017 7 CCGGGTG 30 8.230934E-6 36.996017 11 CGAACTG 55 1.2732926E-11 36.996017 6 TCGCAAG 25 1.2251442E-4 36.996017 12 GCAAGCG 30 8.230934E-6 36.996017 1 CGAACAA 25 1.2251442E-4 36.996017 6 ATTAGAC 25 1.2251442E-4 36.996017 3 TTGGACC 25 1.2251442E-4 36.996017 11 CGACGTT 75 0.0 36.996017 6 CGCGGGG 25 1.2251442E-4 36.996017 9 GAGTCTT 25 1.2251442E-4 36.996017 7 >>END_MODULE