SRR2099148_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2099148_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	246044
Sequences flagged as poor quality	0
Sequence length	43
%GC	49

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TAGAAATGGTACCGGACTGTAGAACTCTGAACCCCTATAGTGA	3561	1.4473021085659474	Illumina DpnII expression PCR Primer 2 (95% over 22bp)
TAGCCATGGCATCGGATTCAGCGAATCCAGCACTCCAGGACGT	3165	1.2863552860463983	No Hit
TAGCCATGCACCCGGTCAAAAAAGGGTCAAAAAAGGCCAAAAA	1601	0.6506966233681781	No Hit
TAGCCATGCGCTCGGTGCTCTGGGGGAAGCAGCACTCAGCCCT	1519	0.6173692510282713	No Hit
TAGCCATGGTAATCCTTTTAAGCCCTTTGGGCATGTGTATAAC	1367	0.5555916827884444	No Hit
TAGAAATGGTACCGGGACTGTAGAACTCTGAACCCCTATAGTG	1255	0.5100713693485718	Illumina DpnII expression Adapter 1 (95% over 21bp)
TGGCCATGCAAATGGACGGCGTGATATAGGTGTTGGGGCCCTG	1240	0.5039748987985888	No Hit
TAGCCATGCCGACGCCGACCTGCGTGTATCCGACCGGCTTGTT	976	0.39667701711888936	No Hit
TACCCATGATACCGCAATCCCTTAGGGAAAGAGCCCTAGCCGC	928	0.3771683113589439	No Hit
TAACCATGGGGTTGGTGGTAGCAAGGTTCCACAGAAAAAAGTA	899	0.36538180162897693	No Hit
TGGCCATGCAAATGGGACGGCGTGATATAGGTGTTGGGGCCCT	867	0.35237599778901335	No Hit
TAGCCATGGTACAAGAAATGCAGAATGCATTTGATGGGCTCAT	858	0.3487181154590236	No Hit
TAGCCATGCGGACGCCAAGGTGACAAACGCACAGGCGAAAGCA	838	0.3405894880590463	No Hit
TAGCCATGTTAGCCGCGTCGAGTTCACCTCCCACTGCAGCACC	786	0.3194550568191055	No Hit
TAGCCAGCGGGTCCGCGGCCGAAGACCGGGCGCGAGGCGGGCA	722	0.29344344913917836	No Hit
TAGCCATGCCGACGGCCGACCTGCGTGTATCCGACCGGCTTGT	692	0.28125050803921253	No Hit
TATCCATGCAAAGGTCGCCGACTCCATCATCAAGGCCGCCGAG	669	0.27190258652923865	No Hit
TAGCCTTTGAAATCCAAGTACAGGTGCTTGCAAATTCAGACCT	628	0.2552389003592853	No Hit
TAGCCATGAGCGCGTACGACGTGCCCCTCCATCCGCCGCTCAC	616	0.25036172391929895	No Hit
TACCCATGATACCTGCAATCCCTTAGGGAAAGAGCCCTAGCCG	585	0.23776235144933427	No Hit
TAGCCATGATAGGGTTGCCAAGGGGCGGGGGGAGTAGGGAGTG	514	0.20890572417941508	No Hit
TAGCCGTTAGTCTGTGACCGAATTGGTGATTCATGTCCGGTGG	497	0.20199639088943439	No Hit
TGGCCATGACTTATCACAGGTGCCCGGCTGAGGATCGGCGTTG	469	0.19061631252946626	No Hit
TAGCCAGAATTGTGGGTGTCCCGCCTGCTGGACTTCCACCAGC	448	0.18208125375949016	No Hit
TGGCCATGACTTACACAGGTGCCCGGCTGAGGATCGGCGTTGT	409	0.16623043032953455	No Hit
TAGCCATGCGCTCGGGTGCTCTGGGGGAAGCAGCACTCAGCCC	359	0.14590886182959145	No Hit
TAGCCTTTGAAATCAAGTACAGGTGCTTGCAAATTCAGACCTA	349	0.14184454812960284	No Hit
TAGCCATGTTGCTACATCTCCTTTCTTCTGCATGGTGACTTGG	345	0.1402188226496074	No Hit
ACGCCATGATCCGCCGGTGACGACAAGGCGCGCGCCGACGCCT	328	0.13330948935962672	No Hit
ACGCCATGATCCGGCGGCTGGACGCCGTCTGGTTCGGCCGGGG	323	0.13127733250963242	No Hit
TAGCCATGTTGCTTACATCTCCTTTCTTCTGCATGGTGACTTG	320	0.13005803839963584	No Hit
TAGAAATGGTACCACTCTGAACCCCTATAGTGAGTCGTATTAC	320	0.13005803839963584	No Hit
TTGCTATGCATGAGTCCGACGATCCATGGACGGTAGTTCAGTG	318	0.1292451756596381	No Hit
TAGCCATGGCCCCCCGACGATCAATCATAGCCATGTTTTTTTT	313	0.1272130188096438	No Hit
TAGCCATGGTACGGGAAAAGAGAACAATTTGCCACTGTAGAAG	308	0.12518086195964948	No Hit
TAGCCATGCTCGTGTTTCTGTAATGAATATTGTCTTGATCATT	304	0.12355513647965405	No Hit
TAGCCATGCGACCGAAGTTTCTCAAGCTGAAGCCGAACTAGAA	298	0.12111654825966087	No Hit
TAGCCATGCACCCGGGTCAAAAAAGGGTCAAAAAAGGCCAAAA	297	0.12071011688966202	No Hit
TAGCCATGCCGACTGCCGACCTGCGTGTATCCGACCGGCTTGT	295	0.11989725414966429	No Hit
TAGCCCTACGTTTGTAGACTTTAAAATTTGAGACTAGCAAACT	289	0.11745866592967112	No Hit
TAGCCATACGACAATTGTTTTTTAGAACGTGAATGGTATCACT	284	0.11542650907967682	No Hit
TACCCATGATACCCAATCCCTTAGGGAAAGAGCCCTAGCCGCA	283	0.11502007770967794	No Hit
TAGCCATGCGGACGGCCAAGGTGACAAACGCACAGGCGAAAGC	276	0.1121750581196859	No Hit
TAGCGACGAGCTACAGGATTTGTGATGGTGTCCAGTTTGGAGC	269	0.10933003852969389	No Hit
TAGAAATGGTACCAGGACTGTAGAACTCTGAACCCCTATAGTG	255	0.10363999934970981	Illumina DpnII expression Adapter 1 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCAGGG	50	1.6734703E-10	37.220787	36
GTACCGA	115	0.0	37.023815	9
GTACCCG	75	0.0	37.023815	9
GTTAGTC	115	0.0	37.023815	6
CGACATA	25	1.2261505E-4	37.023815	9
CGATTAT	25	1.2261505E-4	37.023815	9
GCCATAT	90	0.0	37.023815	3
TGCGATT	130	0.0	37.023815	7
ATGAGCT	300	0.0	37.023815	6
ATGAGCG	200	0.0	37.023815	6
GAGCGCA	45	2.586603E-9	37.023815	8
TGCGAAG	45	2.586603E-9	37.023815	7
CCATAGG	55	1.2732926E-11	37.023815	4
GATATAT	25	1.2261505E-4	37.023815	8
ATGACGC	50	1.7644197E-10	37.023815	6
TGCTCGT	75	0.0	37.023815	7
ATGCGAT	135	0.0	37.023815	6
TGGGACC	90	0.0	37.023815	7
GCCACGA	90	0.0	37.023815	3
ATACCCA	50	1.7644197E-10	37.023815	9

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content