SRR2099147_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2099147_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	61615
Sequences flagged as poor quality	0
Sequence length	43
%GC	49

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TAGAAATGGTACCGGACTGTAGAACTCTGAACCCCTATAGTGA	912	1.4801590521788526	Illumina DpnII expression PCR Primer 2 (95% over 22bp)
TAGCCATGGCATCGGATTCAGCGAATCCAGCACTCCAGGACGT	860	1.3957640185019882	No Hit
TAGCCATGGTAATCCTTTTAAGCCCTTTGGGCATGTGTATAAC	406	0.6589304552462873	No Hit
TGGCCATGCAAATGGACGGCGTGATATAGGTGTTGGGGCCCTG	402	0.6524385295788363	No Hit
TAGCCATGCACCCGGTCAAAAAAGGGTCAAAAAAGGCCAAAAA	396	0.6427006410776597	No Hit
TAGCCATGCGCTCGGTGCTCTGGGGGAAGCAGCACTCAGCCCT	372	0.603749087072953	No Hit
TAGCCATGCCGACGCCGACCTGCGTGTATCCGACCGGCTTGTT	308	0.4998782763937353	No Hit
TAGAAATGGTACCGGGACTGTAGAACTCTGAACCCCTATAGTG	296	0.48040249939138197	Illumina DpnII expression Adapter 1 (95% over 21bp)
TAGCCATGCGGACGCCAAGGTGACAAACGCACAGGCGAAAGCA	276	0.44794287105412645	No Hit
TGGCCATGCAAATGGGACGGCGTGATATAGGTGTTGGGGCCCT	249	0.40412237279883145	No Hit
TACCCATGATACCGCAATCCCTTAGGGAAAGAGCCCTAGCCGC	242	0.39276150288079203	No Hit
TAGCCATGTTAGCCGCGTCGAGTTCACCTCCCACTGCAGCACC	238	0.38626957721334093	No Hit
TATCCATGCAAAGGTCGCCGACTCCATCATCAAGGCCGCCGAG	235	0.38140063296275256	No Hit
TAGCCATGGTACAAGAAATGCAGAATGCATTTGATGGGCTCAT	213	0.3456950417917715	No Hit
TAGCCATGCCGACGGCCGACCTGCGTGTATCCGACCGGCTTGT	195	0.3164813762882415	No Hit
TAACCATGGGGTTGGTGGTAGCAAGGTTCCACAGAAAAAAGTA	194	0.31485839487137873	No Hit
TAGCCAGCGGGTCCGCGGCCGAAGACCGGGCGCGAGGCGGGCA	188	0.30512050637020205	No Hit
TAGCCATGAGCGCGTACGACGTGCCCCTCCATCCGCCGCTCAC	181	0.29375963645216263	No Hit
TAGCCTTTGAAATCCAAGTACAGGTGCTTGCAAATTCAGACCT	177	0.2872677107847115	No Hit
TACCCATGATACCTGCAATCCCTTAGGGAAAGAGCCCTAGCCG	172	0.27915280370039763	No Hit
TGGCCATGACTTATCACAGGTGCCCGGCTGAGGATCGGCGTTG	158	0.25643106386431874	No Hit
TAGCCATGATAGGGTTGCCAAGGGGCGGGGGGAGTAGGGAGTG	145	0.23533230544510267	No Hit
TAGCCAGAATTGTGGGTGTCCCGCCTGCTGGACTTCCACCAGC	137	0.22234845411020043	No Hit
TAGCCGTTAGTCTGTGACCGAATTGGTGATTCATGTCCGGTGG	135	0.2191024912764749	No Hit
TGGCCATGACTTACACAGGTGCCCGGCTGAGGATCGGCGTTGT	129	0.20936460277529825	No Hit
TTGCTATGCATGAGTCCGACGATCCATGGACGGTAGTTCAGTG	103	0.16716708593686602	No Hit
TAGCCATGCGCTCGGGTGCTCTGGGGGAAGCAGCACTCAGCCC	102	0.16554410452000323	No Hit
TAGCCATGCCGACTGCCGACCTGCGTGTATCCGACCGGCTTGT	93	0.15093727176823826	No Hit
TAGCCATGTTGCTACATCTCCTTTCTTCTGCATGGTGACTTGG	92	0.14931429035137547	No Hit
ACGCCATGATCCGCCGGTGACGACAAGGCGCGCGCCGACGCCT	90	0.14606832751764992	No Hit
TAGCCATGAGTTTAAAGCCATGCCACATGGAAGCATTCAGTCA	89	0.14444534610078716	No Hit
TAGCCATGCGGACGGCCAAGGTGACAAACGCACAGGCGAAAGC	88	0.14282236468392437	No Hit
TAGCCATACGACAATTGTTTTTTAGAACGTGAATGGTATCACT	88	0.14282236468392437	No Hit
TAGCCATGTTGCTTACATCTCCTTTCTTCTGCATGGTGACTTG	87	0.14119938326706158	No Hit
TTGTCATGGTCACCAGTCCGACGATCGTCGGACTGTAGAACTC	87	0.14119938326706158	Illumina DpnII expression Sequencing Primer (100% over 21bp)
TAGCCATGGTACGGGAAAAGAGAACAATTTGCCACTGTAGAAG	85	0.13795342043333603	No Hit
TAGAAATGGTACCACTCTGAACCCCTATAGTGAGTCGTATTAC	84	0.13633043901647326	No Hit
ACGCCATGATCCGGCGGCTGGACGCCGTCTGGTTCGGCCGGGG	81	0.13146149476588492	No Hit
TACCCATGATACCCAATCCCTTAGGGAAAGAGCCCTAGCCGCA	80	0.12983851334902213	No Hit
TAGCCTTTGAAATCAAGTACAGGTGCTTGCAAATTCAGACCTA	74	0.12010062484784549	No Hit
TAGCCATGCTCGTGTTTCTGTAATGAATATTGTCTTGATCATT	73	0.1184776434309827	No Hit
TAGCCATGGGTCTGCACAATATTTAAAAATTTATAGATTTAGA	72	0.11685466201411994	No Hit
TAGCCATGGCCCCCCGACGATCAATCATAGCCATGTTTTTTTT	72	0.11685466201411994	No Hit
TAGCCCTACGTTTGTAGACTTTAAAATTTGAGACTAGCAAACT	71	0.11523168059725716	No Hit
TAGAAATGGTACCAGTCGTATTACCGGCCGACGATCGTAACTA	71	0.11523168059725716	No Hit
TCGGCATGAGTCGGCAGCAGATGCTCGGCCGAGCAATCCACGT	70	0.11360869918039437	No Hit
TAGCCATGCGACCGAAGTTTCTCAAGCTGAAGCCGAACTAGAA	68	0.11036273634666882	No Hit
TAGCCATAGCTATGAAAAATGCCAGCTGTGGGACAAGGAGTGC	68	0.11036273634666882	No Hit
TAGCCATGGCCCTGGAGCCACCACTGAGTGGTCCTGAGCTGCT	66	0.10711677351294328	No Hit
TAGCCATGGTCTGGTGCCCTCTATGGAGAGAGTGACCTCTGAC	65	0.1054937920960805	No Hit
TAGAAATGGTACCAGGACTGTAGAACTCTGAACCCCTATAGTG	65	0.1054937920960805	Illumina DpnII expression Adapter 1 (95% over 21bp)
TAGCCATGAGCGCATCCGCCGCTCACCGACAAGCTGCGCTTCG	63	0.10224782926235494	No Hit
TAGCCATGCCGGCGGGACCAATCAGAGTGCTCGCTGTGCAAGC	63	0.10224782926235494	No Hit
TGGCCAAGAGCTGCGTAATCTCCAGATCGAGTATTACCCCACT	62	0.10062484784549217	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGTCTG	20	0.0018284476	37.0	7
TGTGACC	25	1.2201136E-4	37.0	13
GCGGGAC	25	1.2201136E-4	37.0	12
ATGGGGT	50	1.72804E-10	37.0	6
ATGGGGC	30	8.184894E-6	37.0	6
CTTCACA	20	0.0018284476	37.0	36
ACCGGGA	30	8.184894E-6	37.0	11
CATGATT	35	5.5168493E-7	37.0	5
CATGATA	155	0.0	37.0	5
TGCACCC	65	0.0	37.0	7
GAGCGCG	30	8.184894E-6	37.0	8
CATGAGG	35	5.5168493E-7	37.0	5
TCAAGTA	20	0.0018284476	37.0	13
GGTTGCC	20	0.0018284476	37.0	13
CATGACG	40	3.7334758E-8	37.0	5
CATGACC	40	3.7334758E-8	37.0	5
CATGAAT	45	2.5356712E-9	37.0	5
CATGAAC	35	5.5168493E-7	37.0	5
GCCTTGA	20	0.0018284476	37.0	17
CCTACGT	60	0.0	37.0	5

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content