SRR2099146_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2099146_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	213634
Sequences flagged as poor quality	0
Sequence length	43
%GC	49

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGAAGGCTCAGGAGGAAGACGTGCATGTACGGATCGTCCTCGC	3440	1.6102305812745163	No Hit
CGTAAGCTCTGGCACCTTACGCGTAAGCTTTTTTTTTTTTTTT	2388	1.117799601187077	No Hit
CGTAAGCTAAGCGCGCACAGCAGACGGAACAGGCTGGTCTTGC	2372	1.1103101566230094	No Hit
CGTAAGCTTGCCGGGGAAGGGGAAGGGGAAGGGGAAGGGGAAA	2340	1.0953312674948745	No Hit
CGTAAGCTTATCGGGTGCTCAGAGGCCTCCATGCAGATGGCTG	1404	0.6571987604969246	No Hit
CGTAAGCTTGCCGGGGGAAGGGGAAGGGGAAGGGGAAGGGGAA	1188	0.5560912588820132	No Hit
CGTAAGCTCTAACGGCAAGAAAAATGTTGGAAACAATAGGAGG	836	0.39132347847252774	No Hit
CGAAAGCACGCACGAGATGTTGAGCATTTTTTCATGTACTTGT	807	0.3777488602001554	No Hit
CGAAGGCTCAGGAGGGAAGACGTGCATGTACGGATCGTCCTCG	788	0.3688551447803252	No Hit
CGTAAGCTCTGCCTTGGAAGAGGTGAGGAAAAAAGAGCATCGG	769	0.359961429360495	No Hit
CGTAAGCTGTTAGGGCAAATCCCCATCTCTACTAAAAATAGCC	664	0.31081194940880197	No Hit
CATAAGCTTGTTTGGTGGAAAGTGGAGCAATAAGAGCTAAATT	653	0.30566295627100554	No Hit
CGTAAGCTGCAATGCTCCTGACCACCACCTCTAATGGTGCTGT	644	0.3014501437037176	No Hit
CGTAAGCCCGGGAACCTTGGAAAACGTTCGAATTCAAGGCTTC	576	0.2696200043064306	No Hit
CGAAAGGTCATGGGCCTTCACCATGTACTAGACTAGATAACCT	492	0.23030042034507614	No Hit
CGAAAGCACGCACGGAGATGTTGAGCATTTTTTCATGTACTTG	428	0.2003426420888061	No Hit
CGTAAGCTCCTACGCTCCCCGCGACCAAGGCCCTCCCGCCGCT	384	0.17974666953762042	No Hit
CGTAAGCTGAGGCAGTCAAACGTTCTTTGCACGTTTGACTTTA	349	0.16336350955372272	No Hit
CGTAAGCTGGATTAACGTAAGCTTTTTTTTTTTTTTTTTTTTT	333	0.1558740649896552	No Hit
CGTAAGCTCTGCCGTTGGAAGAGGTGAGGAAAAAAGAGCATCG	333	0.1558740649896552	No Hit
CGTGAGATAGGTGTAATACGGACGATGGAATCCTCAACGTCAC	330	0.15446979413389256	No Hit
CGTAAGCTCGTCCGGGTACGGAGGGGAGATGTGAGATCTATGC	325	0.15212934270762143	No Hit
CGTAAGCTGAGGCAAGTCAAACGTTCTTTGCACGTTTGACTTT	307	0.14370371757304548	No Hit
CGTAAGCTTATCGGGGTGCTCAGAGGCCTCCATGCAGATGGCT	304	0.14229944671728284	No Hit
CGTAAGCTTGCGTAGTTTACAAGGAAAAAACTGAATCCTTGTA	298	0.1394909050057575	No Hit
CGTAAGCTCCACTTCTCACTGTAGTGTCCTCTGGGGTCAGCCC	282	0.13200146044169	No Hit
CGTATGCTTGCTCGTCGTGGTGGGCGGCGCCGGTTCGCTCGAA	278	0.13012909930067312	No Hit
AGTCAGCTAATCATCTTCTACGGATCACGCTAACGGTCTTGTG	277	0.12966100901541888	No Hit
CGAAGGCTCAGGATGGAAGACGTGCATGTACGGATCGTCCTCG	271	0.12685246730389357	No Hit
CGTAAGCTCAAGCAGAAAAGGTCATTGTAGAGCCTCAAAAGTA	269	0.12591628673338512	No Hit
CGTAAGCTCCACTCTCACTGTAGTGTCCTCTGGGGTCAGCCCC	268	0.1254481964481309	No Hit
CGTAAGCTGCAATGGCTCCTGACCACCACCTCTAATGGTGCTG	266	0.12451201587762248	No Hit
CGTAAGCCCGGGACCTTGGAAAACGTTCGAATTCAAGGCTTCT	266	0.12451201587762248	No Hit
CGTAAGCTCTAACGGGCAAGAAAAATGTTGGAAACAATAGGAG	259	0.12123538388084294	No Hit
CGTAAGCTTCTGACTGCGCCAAGAAATGAGATCAGAATCCAGG	249	0.11655448102830075	No Hit
CGTAAGCTGAGGCTCAAACGTGTTCTTTGCACGTTTGACTTTA	235	0.11000121703474165	No Hit
CGTAAGCTAGGTGGTGGGCTGTCTGTGATCTGCAGCCAGGGTG	232	0.108596946178979	No Hit
CGTAAGCTTCTGAGCTGCGCCAAGAAATGAGATCAGAATCCAG	231	0.10812885589372477	No Hit
CGTAAGCTCTCGTCAGTCCGACGATCATCGGCGTAAGCTTACG	230	0.10766076560847057	No Hit
GGTACGCTTCAGACAAAACATGGATCTGACTTTTTTTTTTTCT	226	0.10578840446745369	No Hit
CGTAAGCTTGCCGAGGGAAGGGGAAGGGGAAGGGGAAGGGGAA	224	0.10485222389694523	No Hit
CGAAGGCTCATGGCTCCAAGCCCTGGAGGGGCTGAAAATGGTG	223	0.10438413361169101	No Hit
CGTAAGCTGCTATGGAGGCTGAGGCATGAGAATCGCTTGAACC	219	0.10251177247067415	No Hit
CGTAAGCTCAGTTGACTAGAAGGAATTTTTTCCTTCTAGTCGA	217	0.1015755919001657	No Hit
CGAAGGCTCAGGAAGGAAGACGTGCATGTACGGATCGTCCTCG	216	0.10110750161491147	No Hit
CGTAAGCTGGGGACAGTCCGACGATCCTCGTCGTACTGTAGAA	215	0.10063941132965727	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTATGC	30	7.931561E-6	37.232674	37
CTTCGTT	25	1.2240355E-4	37.03189	7
CTTCGCA	25	1.2240355E-4	37.03189	7
ATCTAGC	50	1.7644197E-10	37.03189	5
TAGCGCG	25	1.2240355E-4	37.03189	8
TCTAGCT	50	1.7644197E-10	37.03189	6
GGCTTCG	25	1.2240355E-4	37.03189	5
GCTCTAA	175	0.0	37.03189	6
CTAGTGT	25	1.2240355E-4	37.03189	7
CTAGTAG	25	1.2240355E-4	37.03189	7
CTACGGG	25	1.2240355E-4	37.03189	7
CAGGACG	25	1.2240355E-4	37.03189	9
GCAATCG	25	1.2240355E-4	37.03189	9
TCGAGCG	25	1.2240355E-4	37.03189	9
GCTCCTA	195	0.0	37.03189	6
TCTGCCT	130	0.0	37.031887	8
TCTGCCG	55	1.2732926E-11	37.031887	8
CTTAAAT	20	0.0018308162	37.031887	7
CTTCGAA	65	0.0	37.031887	7
TGTCACG	30	8.231475E-6	37.031887	8

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content