FastQCFastQC Report
Thu 26 May 2016
SRR2099145_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099145_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56525
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAAGGCTCAGGAGGAAGACGTGCATGTACGGATCGTCCTCGC10351.8310482087571869No Hit
CGTAAGCTTGCCGGGGAAGGGGAAGGGGAAGGGGAAGGGGAAA7351.3003095975232197No Hit
CGTAAGCTAAGCGCGCACAGCAGACGGAACAGGCTGGTCTTGC6461.1428571428571428No Hit
CGTAAGCTTATCGGGTGCTCAGAGGCCTCCATGCAGATGGCTG4450.7872622733303848No Hit
CGTAAGCTTGCCGGGGGAAGGGGAAGGGGAAGGGGAAGGGGAA3800.6722689075630253No Hit
CGAAAGCACGCACGAGATGTTGAGCATTTTTTCATGTACTTGT2520.4458204334365325No Hit
CGAAGGCTCAGGAGGGAAGACGTGCATGTACGGATCGTCCTCG2300.4068996019460416No Hit
CGTAAGCTCTAACGGCAAGAAAAATGTTGGAAACAATAGGAGG2120.37505528527200355No Hit
CGTAAGCTCTGCCTTGGAAGAGGTGAGGAAAAAAGAGCATCGG2060.36444051304732417No Hit
CGTAAGCTAGGTGGTGGGCTGTCTGTGATCTGCAGCCAGGGTG1960.34674922600619196No Hit
CGTAAGCTGCAATGCTCCTGACCACCACCTCTAATGGTGCTGT1810.3202122954444936No Hit
CGTAAGCTGTTAGGGCAAATCCCCATCTCTACTAAAAATAGCC1570.27775320654577623No Hit
CGTAAGCCCGGGAACCTTGGAAAACGTTCGAATTCAAGGCTTC1440.2547545333923043No Hit
CGAAAGGTCATGGGCCTTCACCATGTACTAGACTAGATAACCT1380.24413976116762492No Hit
CGTAAGCTTTCTAGGGACAGAAGGAAAGCTAGGCCATTTGTGT1350.2388323750552853No Hit
CGTAAGCTAGCTCGTGACCTGGCACAGTGTGTTTTCTGGACCA1270.2246793454223795No Hit
CGTAAGCTGCGTCGGGCCCTGTTCTGGGGACTTTTAGTACCCA1190.21052631578947367No Hit
CGAAAGCACGCACGGAGATGTTGAGCATTTTTTCATGTACTTG1170.20698805838124723No Hit
CGTAAGCTCGTCCGGGTACGGAGGGGAGATGTGAGATCTATGC1150.2034498009730208No Hit
CATAAGCTTGTTTGGTGGAAAGTGGAGCAATAAGAGCTAAATT1110.19637328615656788No Hit
CGTAAGCTCCTACGCTCCCCGCGACCAAGGCCCTCCCGCCGCT1080.19106590004422822No Hit
CGTAAGCTTATCGGGGTGCTCAGAGGCCTCCATGCAGATGGCT1050.18575851393188852No Hit
CGTAAGCTTGGTCGGAGCCAAGGGATGGACATCTTGTTTTTGT1040.1839893852277753No Hit
CGTGAGATAGGTGTAATACGGACGATGGAATCCTCAACGTCAC1000.17691287041132242No Hit
CGTAAGCTCCTACTAGACCTGGAACGTCACCTCGCCGCGATCG990.1751437417072092No Hit
CGTAAGCTCTGGCACCTTACGCGTAAGCTTTTTTTTTTTTTTT960.16983635559486954No Hit
CGTAAGCTATTCGCTGGCCTGCTGCTAATGCAGGACAGTCTAC950.16806722689075632No Hit
CGTAAGCTGATTCGTGACCAAGGCCTGATGGAATCTTTGCTTG910.1609907120743034No Hit
CGTAAGCTCCACTTCTCACTGTAGTGTCCTCTGGGGTCAGCCC900.15922158337019018No Hit
CGTAAGCTGAGGCAGTCAAACGTTCTTTGCACGTTTGACTTTA880.15568332596196374No Hit
CGAAGGCTCAGGATGGAAGACGTGCATGTACGGATCGTCCTCG850.15037593984962408No Hit
CGTAAGCTTCTGACTGCGCCAAGAAATGAGATCAGAATCCAGG820.1450685537372844No Hit
CGTAAGCTCTGCCGTTGGAAGAGGTGAGGAAAAAAGAGCATCG810.14329942503317117No Hit
AGTCAGCTAATCATCTTCTACGGATCACGCTAACGGTCTTGTG800.14153029632905795No Hit
TGTAGGCTTCGAAGCCTACATTCGAGATCGTCGGACTGTAGAA800.14153029632905795No Hit
CGTATGCTTGCTCGTCGTGGTGGGCGGCGCCGGTTCGCTCGAA790.1397611676249447No Hit
CGTAAGCTGAGGCAAGTCAAACGTTCTTTGCACGTTTGACTTT790.1397611676249447No Hit
CGTAAGCTGGATTAACGTAAGCTTTTTTTTTTTTTTTTTTTTT790.1397611676249447No Hit
CGAAGGCTCAGGAAGGAAGACGTGCATGTACGGATCGTCCTCG730.12914639540026537No Hit
CGTAAGCTGCAATGGCTCCTGACCACCACCTCTAATGGTGCTG730.12914639540026537No Hit
CGTAAGCTTAATGGTTGCCGCTGCCTATAAGTTTGGCGTGGCT730.12914639540026537No Hit
CGTAAGCTTCTGAGCTGCGCCAAGAAATGAGATCAGAATCCAG710.12560813799203893No Hit
CGTAAGCCCGGGACCTTGGAAAACGTTCGAATTCAAGGCTTCT700.12383900928792571No Hit
CGTAAGCTTGCCGAGGGAAGGGGAAGGGGAAGGGGAAGGGGAA700.12383900928792571No Hit
CGTAAGCTTGCGTAGTTTACAAGGAAAAAACTGAATCCTTGTA680.12030075187969924No Hit
CGTAAGCTCCACTCTCACTGTAGTGTCCTCTGGGGTCAGCCCC680.12030075187969924No Hit
CGTAAGCTCTGACCAGTCCGACGATCTGATTCGTAAGCTTACG640.11322423706324634No Hit
CGTAAGCTCTTGTGAGTTCTAAAGTCCGACGATCAGGATCGTA630.11145510835913312Illumina DpnII expression Sequencing Primer (95% over 21bp)
CGTAAGCTGGCAAGTGCCCCAGGGCAGCCACAGAATCTGGCAC630.11145510835913312No Hit
CGTAAGCTTCCTTGAGGCAATGCCTTCTGTGGCCACTAGGGAC630.11145510835913312No Hit
CGTAAGCTTCTCAGAGACTCTGGTCAGAGGATGGTTTCCTTGG620.1096859796550199No Hit
CGTAAGCTGGATTGAACGTAAGCTTTTTTTTTTTTTTTTTTTT620.1096859796550199No Hit
CGTAAGCTACGATGTGGCTACTGCGGCCTGCAGTTCAAGCAGC620.1096859796550199No Hit
CGAAAGGTCATGGGGCCTTCACCATGTACTAGACTAGATAACC610.10791685095090668No Hit
CGTATGCTTGCTCGGTCGTGGTGGGCGGCGCCGGTTCGCTCGA610.10791685095090668No Hit
TGTAGGCTTCGAAGGCCTACATTCGAGATCGTCGGACTGTAGA600.10614772224679346No Hit
CGTAAGCTCCACTCCTCACTGTAGTGTCCTCTGGGGTCAGCCC600.10614772224679346No Hit
CGTAAGCTTGATTGTAGGGCTGGTATGCAACAGAGGCTGCTCC590.10437859354268024No Hit
CGTAAGCTCAAGCAGAAAAGGTCATTGTAGAGCCTCAAAAGTA580.102609464838567No Hit
CGTAAGCTCGTCACAGTCCGACGATCCTTCCCGTAAGCTTACG570.10084033613445378No Hit
CGTAAGCTCTCGTCAGTCCGACGATCATCGGCGTAAGCTTACG570.10084033613445378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCTA7950.037.0000043
CTAAGCG1050.037.0000047
TGTTGTG200.001827164637.08
CAGCTAA1200.037.04
CTCTGCC600.037.07
TCCTTGA200.001827164637.09
AGCTCTT600.037.05
GGTTGCC308.17285E-637.013
ATCTTTG308.17285E-637.033
AATCTGG355.5060264E-737.04
GCCTTGG403.724017E-837.011
CCTACGC308.17285E-637.09
GTCATGG355.5060264E-737.07
CAGTCAA308.17285E-637.013
AGCTTGG1550.037.05
TCAAGCG251.2188306E-437.011
GCAAGTC251.2188306E-437.012
AGCTTCG850.037.05
AGCTTCA200.001827164637.05
AGCTCAG900.037.05
Produced by FastQC (version 0.11.5)