FastQCFastQC Report
Thu 26 May 2016
SRR2099143_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099143_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83854
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTACCCCACTGGCAGGCGCCGAGATAGGTGTTCATGGTG7830.9337658310873661No Hit
ATCGGTACGGAGGGATCAGGATATCGGTACTTTTTTTTTTTTT4130.49252271805757625No Hit
ATCGGTACTGAGGGATGCCTCGCCTTTCTGTACTCATAGCGGG3990.4758270326996923No Hit
ATCGGTACTGAGAGTTTTGATGAGTCTGGATTCCAGACAAGAA2750.32795096238700594No Hit
ATCGGTAATGCGCCAGACCTACTCCCGCGTCTCCGAGTATATC2680.31960311970806404No Hit
ATCGGTACGGCCGGAAATATAAGAGGGCCTGAACTCTGCTGCC2680.31960311970806404No Hit
ATCGGTACTTTTAGGACGTCGTCCGCGAGGAGGTCGCGCGGAT2660.31721802179979486No Hit
ATCGGTACCATTCGGGGCCGCCCTCTCGCCCGTCACGTTGAAC1910.22777685023970232No Hit
ATCGGTACCCAGGATCAAGGAATCGGTACTTTTTTTTTTTTTT1850.22062155651489496No Hit
ATCGGTACCCCACGTGGCAGGCGCCGAGATAGGTGTTCATGGT1800.2146588117442221No Hit
ATCGGTACGGTGCGGCCAGACTGGGGATAGATCCCCTTGCTTT1600.19080783266153076No Hit
ATCGGGATTTCAGCGCGGCCAACGACAGCGACATCGCGCGCAG1580.18842273475326163No Hit
ATCGGTAATGCGCGCAGACCTACTCCCGCGTCTCCGAGTATAT1510.18007489207431965No Hit
ATCGGTACGGCGCGTCTCCAAGGCTGAGTTCCTGGACCCCCAA1350.16099410880816659No Hit
ATCGGTACTAACATCTCGTTAGCCATTATCTGCTGACGTTCTT1310.1562239129916283No Hit
ATCGGTACCCTTACAGTCCGACGATCCTGAGATCGTCGGACTG1250.1490686192668209No Hit
ATCGGTACGGCTGGCTTGAGCAGGATAGCAAATATATAAATTG1120.13356548286307154No Hit
ATCGGTACTCAGAGGAGAAACAAGCTTGACCACTATGCTATCA1110.13237293390893698No Hit
ATCGGTCGCTTGAAGTTGAACATGGGTCAGTCGGTCCTGAGAG1100.1311803849548024No Hit
ATCGGTACACTCCGGAGGGAATGGGAGGAAGTGGGAACAGGGT1080.12879528704653326No Hit
ATCGGTACAGCAACAGTCCGACGATCCGTCGATCGGACTGTAG1060.12641018913826413No Hit
AGCGGTACGTTGCGGCGAGGGCCTTCTCCAGGGCGCCGGTGCC1050.12521764018412956No Hit
ATCGGTACTACGGGGATACTGTGTATCCTTTAATAGACCAGGT1020.12163999332172587No Hit
ATCGGTACATCAGGCAAAACAAGACAGCAGCCACTAGGTACCG990.11806234645932215No Hit
ATCGGTACCGCTAAACTAGACCGGGCGCGGTGGCTCACACCTG960.11448469959691844No Hit
ATCGGTACTCGACGTTGTGAGTACACCTGGAACAGAGTGTGAG960.11448469959691844No Hit
ATCGGTACTGTTCTTCAGAGTCAACCCTGAATTTCAGGATTTT950.11329215064278389No Hit
ATCGGTACCATCAGTGGGCTCCTCTGACCCCCTGAGCATGGTA920.10971450378038018No Hit
ATCGGTACCCAGGCATCAAGGAATCGGTACTTTTTTTTTTTTT920.10971450378038018No Hit
ATCGGTACTGCTCAACGCCAAGGGAAAAGGCTCCAAGGAACTG880.10494430796384191No Hit
ATCTGAACTTACATTTCATCTCCATCCAGAAACATCTAGCTTT860.1025592100555728No Hit
ATTGGTACAGCTACCCATCATCATCTTGCGTAGCATGGACATG840.10017411214730365No Hit
ATCGGTACGACCAGAGACTGTCTAAGACACAGAAGCACGTCAG840.10017411214730365No Hit
ATCGGTACCTCCTCGCAGGTAAAGCTGCGTGGATGCGGCCAGC840.10017411214730365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTTAC1050.037.0000046
ACAACTG200.001832222937.07
AACGTTA403.7613063E-837.01
GTGCACT200.001832222937.05
GGTACTA2250.037.04
GATACGG200.001832222937.04
GGTACGT4300.037.04
ACAGTGT403.7613063E-837.07
GGTACGG6250.037.04
GGTACGC3850.037.04
GTACCCA1650.037.05
TACCCGG355.5487544E-737.06
TACCCCG403.7613063E-837.06
CCGGGAC200.001832222937.012
ACTGCGG200.001832222937.07
AGTTTTG355.5487544E-737.013
GTCGATC200.001832222937.028
GTACATG800.037.05
TACTTGT308.220375E-637.06
TACTTGG200.001832222937.06
Produced by FastQC (version 0.11.5)