FastQCFastQC Report
Thu 26 May 2016
SRR2099142_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099142_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311627
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACAGTAGCTGTCGGCGATCGCGGCGCGCCGCAGCAGGTGA47831.5348477506762892No Hit
TCGACAGTTTGGTCAGTCCGACGATCGCTTATCGACAGTTTTT17570.5638150737901402No Hit
GCTACAGTTCGCTGATGGTGTGCCCATGCCTGACAAGTACTCC13970.4482923495075844No Hit
GCTACAGTCCATTTTGTAAGGGCGCACGGTGGATGCCTTGGCA7930.25447088987796307No Hit
GCTACAGTACTTTGCCATCGCCGGCCAGCCCTTCAACCTGGGA6660.21371703992272814No Hit
TCGACAGTCGCATTCTGGCTAGGCCAGCTCAGGGCAGGGGGGT6540.20986628244664293No Hit
CCGACAGTCGCCCGCAGTTTTTTTTTTTTTTTTTTTTTTTTTT6460.20729911079591948No Hit
TCGACAGTACCGGTGGGCCAGGCGCAGTGGCTCACACCTGTAA6370.20441104268885557No Hit
TCGACAGTGTAAAGACAGTTTTTTTTTTTTTTTTTTTTTTTTT6270.20120207812545127No Hit
GCTACAGTCCTCTGTCTAGGGGAAATGGTTGGAAACAATAATA5890.18900801278451482No Hit
GCTACAGTATGCTAATCAACCAGCCAGGTCTCTCCATGTCGTC5690.18259008365770615No Hit
GCTACAGTCATGTGTGAAGAGTTTAAAGAGGCTTTAATCGGTA5240.1681497431223867No Hit
GCTACAGTGTCTTGTTCTACAGTCCGATGATCTCGAGTCGAGC4750.1524258167617055No Hit
TAGCCAGTGCGCCGACGAACGTCGGCGCTTCCATATCCGTTTC4660.1495377486546416No Hit
TTGACAGTTCGCTCAGAAGTGGTCCTGCAACTTTATCCGCCTC4230.135739201032003No Hit
GCTACAGTCCTGTGACTGGCTCAGCGTGTGCCTACCCTACGCC3840.12322423923472614No Hit
TCGACAGTAGCTGTTCGGCGATCGCGGCGCGCCGCAGCAGGTG3650.11712720656425792No Hit
GCCACAGTCTGATGCGTCCCGGCAAGTTCAGGTTCGTCTACAC3590.11520182782621533No Hit
GCTACAGTCCGCTATTCTTGGACCGGCGCAAGACGGACCAGAG3480.11167196680647055No Hit
TCGATAGTTTTGCGTAAAAGTGTTTCTATTTCTCCACCATTTG3160.10140328020357672No Hit
GCTACAGTCGTATGGGCGCCATGCTGAGGGGAAGGGAAGATGG3150.10108238374723628No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTC200.001835409437.0183148
TCCGCTA403.801324E-837.0183148
TTCCGTA200.001835409437.0183148
GTCTAGT200.001835409437.0183147
CAACACG308.264415E-637.0183149
TAACGAA403.801324E-837.0183148
TCGGCTC200.001835409437.0183148
CGCCTAC200.001835409437.0183149
ACGGCGT200.001835409437.0183144
GTAGAGT403.801324E-837.0183147
GTGTCGT452.593879E-937.0183147
GACGGTA200.001835409437.0183143
TTGCGCG308.264415E-637.0183148
AACGAAT251.2279776E-437.0183149
GTATATC501.7826096E-1037.0183147
CAGTCGC8150.037.0183145
TTGCACG950.037.0183148
AGTAGCG452.593879E-937.0183146
TTACATA200.001835409437.0183148
TCGAATA200.001835409437.0183148
Produced by FastQC (version 0.11.5)