FastQCFastQC Report
Thu 26 May 2016
SRR2099141_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099141_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78982
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACAGTAGCTGTCGGCGATCGCGGCGCGCCGCAGCAGGTGA14291.8092729989111445No Hit
TCGACAGTTTGGTCAGTCCGACGATCGCTTATCGACAGTTTTT5920.7495378693879619No Hit
TCGACAGTACCGGTGGGCCAGGCGCAGTGGCTCACACCTGTAA5370.6799017497657693No Hit
GCTACAGTTCGCTGATGGTGTGCCCATGCCTGACAAGTACTCC3580.45326783317717956No Hit
GCTACAGTCCATTTTGTAAGGGCGCACGGTGGATGCCTTGGCA2580.32665670659137525No Hit
GCTACAGTATGCTAATCAACCAGCCAGGTCTCTCCATGTCGTC2130.2696816996277633No Hit
GCTACAGTACTTTGCCATCGCCGGCCAGCCCTTCAACCTGGGA1980.25069003063989265No Hit
TCGACAGTCGCATTCTGGCTAGGCCAGCTCAGGGCAGGGGGGT1950.24689169684231851No Hit
GCTACAGTCCTCTGTCTAGGGGAAATGGTTGGAAACAATAATA1810.2291661391203059No Hit
TTGACAGTTCGCTCAGAAGTGGTCCTGCAACTTTATCCGCCTC1470.1861183560811324No Hit
TCGACAGTCCGATGTGGCCATCAGGTGCTGCATCTGAGGGTCG1460.18485224481527437No Hit
GCTACAGTCATGTGTGAAGAGTTTAAAGAGGCTTTAATCGGTA1420.17978779975184217No Hit
CCGACAGTCGCCCGCAGTTTTTTTTTTTTTTTTTTTTTTTTTT1360.17219113215669393No Hit
TCGACAGTGTAAAGACAGTTTTTTTTTTTTTTTTTTTTTTTTT1320.16712668709326176No Hit
GCTACAGTGTCTTGTTCTACAGTCCGATGATCTCGAGTCGAGC1240.1569977969663974No Hit
TAGCCAGTGCGCCGACGAACGTCGGCGCTTCCATATCCGTTTC1180.14940112937124916No Hit
TCGACAGTAGCTGTTCGGCGATCGCGGCGCGCCGCAGCAGGTG1130.14307057304195892No Hit
TCGATAGTTTTGCGTAAAAGTGTTTCTATTTCTCCACCATTTG1120.14180446177610087No Hit
TCGACAGTACACCGAGGTCTCTGGGGACCTTCTGAGGTGGAGG1020.12914334911752046No Hit
GCCACAGTCTGATGCGTCCCGGCAAGTTCAGGTTCGTCTACAC990.12534501531994632No Hit
GCTACAGTCGTATGGGGCGCCATGCTGAGGGGAAGGGAAGATG960.12154668152237218No Hit
GCTACAGTTGGGTAAGTCCAATCACCAGGAATTTGAGGCAGTG960.12154668152237218No Hit
CCAACAGTAGCTAAAAAGTGCTTGGATGAGGTGTGGGTAGCGG950.12028057025651415No Hit
TCGACAGTAGCTCAAAGGCCAAAGATAAAAAGATTTTTTTTTT950.12028057025651415No Hit
GCTACAGTCCGCTATTCTTGGACCGGCGCAAGACGGACCAGAG940.1190144589906561No Hit
GCTACAGTGTGAGAAACGCCCTGCTGTGTTGCAGCAACGGCAT930.11774834772479804No Hit
TCGACAGTGGCACGAAGGATGTGTGTCTCTGCACACACTGAAG920.11648223645894001No Hit
GCTACAGTCCTCTGGTCTAGGGGAAATGGTTGGAAACAATAAT910.11521612519308196No Hit
TCGACAGTAGCTGGTCGGCGATCGCGGCGCGCCGCAGCAGGTG910.11521612519308196No Hit
GCTACAGTCTCCTGTGGACTCTAAGTAAGGGTAAAATGGGAGA880.11141779139550784No Hit
TCGAGACTAGTCTAAACAGCAACAACAAAAAACTACCCATCAG850.1076194575979337No Hit
TCGACAGTGCACACAGTCCGACGATCACAGTTCGACAGTAGAA840.10635334633207567No Hit
TCGACAGTCAGAGGGAGAAATGACCGCCTTTCTTACAACCAAA830.10508723506621762No Hit
TCGACAGTCATGAGAAGAAACCCAAGTTTGAATTGGGCAAGCT810.10255501253450153No Hit
GCTACAGTTCGCTGGATGGTGTGCCCATGCCTGACAAGTACTC810.10255501253450153No Hit
TCGACAGTCCAACGAGGCCAGAACCCACTGCCTCTGGGCCCTC800.1012889012686435No Hit
TCGACAGTTGCAATGAGGATCTGCTTTGACTCAGGTTGGTGAT790.10002279000278544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCAT1050.037.0000045
TTGGTCA1050.037.0000049
TCCGGCC200.001831573937.013
GTGTTTC251.2232414E-437.020
ACAACGG200.001831573937.09
GTCATGT251.2232414E-437.07
GTTTGAT650.037.07
GGAATGC200.001831573937.09
CTGTCTA251.2232414E-437.012
GTCGCCT308.214271E-637.07
ACCGGTG501.7462298E-1037.09
AGTCCCG200.001831573937.06
ACAGTAT3250.037.04
AGTCATC251.2232414E-437.06
CGTATGG200.001831573937.09
GTATTAG452.5538611E-937.07
GACACTA200.001831573937.03
GGGTCCG200.001831573937.012
TTTAACC200.001831573937.024
ATGTGGC251.2232414E-437.012
Produced by FastQC (version 0.11.5)