##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099141_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 78982 Sequences flagged as poor quality 0 Sequence length 43 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.497379149679674 37.0 35.0 37.0 35.0 37.0 2 35.992719860221314 37.0 37.0 37.0 35.0 37.0 3 35.775910967055786 37.0 37.0 37.0 35.0 37.0 4 35.927996252310656 37.0 35.0 39.0 32.0 39.0 5 37.50517839507736 39.0 37.0 39.0 35.0 39.0 6 36.69057506773695 37.0 37.0 39.0 33.0 39.0 7 37.0509863006761 39.0 37.0 39.0 34.0 39.0 8 36.85701805474665 39.0 37.0 39.0 33.0 39.0 9 32.02247347496898 33.0 31.0 34.0 30.0 34.0 10 32.252817097566535 34.0 31.0 34.0 30.0 34.0 11 32.282317490061025 34.0 31.0 34.0 30.0 34.0 12 35.67831911068345 37.0 35.0 37.0 35.0 37.0 13 35.57099085867666 37.0 35.0 37.0 35.0 37.0 14 31.60209921247879 31.0 31.0 34.0 30.0 34.0 15 31.490352232154162 31.0 31.0 34.0 28.0 34.0 16 31.245030513281506 31.0 30.0 34.0 27.0 34.0 17 34.01753564103213 35.0 33.0 37.0 28.0 37.0 18 34.80028360892355 35.0 35.0 37.0 32.0 37.0 19 34.57438403686916 35.0 35.0 37.0 30.0 37.0 20 34.132789749563194 35.0 33.0 37.0 28.0 37.0 21 34.281241295485046 35.0 35.0 37.0 30.0 37.0 22 35.97904585855005 38.0 35.0 39.0 30.0 39.0 23 36.32420045073561 39.0 35.0 39.0 31.0 39.0 24 36.645033045504036 39.0 35.0 39.0 32.0 39.0 25 36.862854827682256 39.0 37.0 39.0 32.0 39.0 26 36.949228938239095 39.0 37.0 39.0 33.0 39.0 27 38.36802056164696 40.0 37.0 41.0 34.0 41.0 28 38.53907219366438 40.0 38.0 41.0 34.0 41.0 29 38.67191258767821 40.0 38.0 41.0 35.0 41.0 30 38.76613658808336 40.0 38.0 41.0 36.0 41.0 31 38.81400825504545 40.0 38.0 41.0 36.0 41.0 32 38.90966296118103 40.0 38.0 41.0 36.0 41.0 33 38.978450786255095 40.0 38.0 41.0 36.0 41.0 34 38.96067458408245 40.0 38.0 41.0 36.0 41.0 35 38.897634904155375 40.0 38.0 41.0 36.0 41.0 36 38.856017826846625 40.0 38.0 41.0 35.0 41.0 37 38.75736243701096 40.0 38.0 41.0 35.0 41.0 38 38.64783115140158 40.0 38.0 41.0 35.0 41.0 39 38.58556379934669 40.0 38.0 41.0 35.0 41.0 40 38.52061229140817 40.0 38.0 41.0 35.0 41.0 41 38.43290876402218 40.0 38.0 41.0 35.0 41.0 42 38.39774885416931 40.0 38.0 41.0 35.0 41.0 43 38.3680079005343 40.0 38.0 41.0 35.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2632452957149951 1101 2 -0.33916367736176767 1101 3 -0.3252084424456356 1101 4 0.4714824778878821 1101 5 -0.009198508895877922 1101 6 -0.8070452711985183 1101 7 -0.12516133594784407 1101 8 -0.23205774204723184 1101 9 -0.12105130562916955 1101 10 0.06860596572285971 1101 11 0.26891202338077136 1101 12 -0.050037997450282035 1101 13 -0.4880796491776209 1101 14 -0.14069504146229406 1101 15 0.002547362941534459 1101 16 -0.08941033150842159 1101 17 -0.25447117481683534 1101 18 -0.6823934137828047 1101 19 -0.7882024105706904 1101 20 -0.6083719659842828 1101 21 -0.5822688274002843 1101 22 -0.9956278037796196 1101 23 0.01921252160923359 1101 24 -0.22846504711655058 1101 25 -0.08559471583004807 1101 26 -0.39434545803323573 1101 27 -0.3037798074564506 1101 28 -0.5013659609282115 1101 29 -0.1455251267563682 1101 30 -0.2915249096699668 1101 31 -0.07758975743793428 1101 32 -0.09345310036269439 1101 33 -0.13364964807597346 1101 34 -0.022392022341080065 1101 35 -0.07647900837743293 1101 36 -0.26455047454258107 1101 37 0.036386980987074935 1101 38 0.014653286914416697 1101 39 -0.3109646067266283 1101 40 -0.23187558643169126 1101 41 0.21488842156129806 1101 42 0.11393307380618012 1101 43 -0.12708688268778445 1102 1 0.643534365301953 1102 2 0.5082939497568759 1102 3 0.7256390151814855 1102 4 0.5986011219556815 1102 5 0.6348692877142881 1102 6 0.7183784576150387 1102 7 0.756194596255547 1102 8 0.9713320884612457 1102 9 0.26877920284540835 1102 10 0.433012745383877 1102 11 0.3028103284655188 1102 12 0.5431823415327699 1102 13 0.6220898423477976 1102 14 0.1559151280292319 1102 15 0.4432253290432264 1102 16 0.3343184820509002 1102 17 0.51671526586113 1102 18 0.08031845062397025 1102 19 -0.08481258006221282 1102 20 0.1712890509648659 1102 21 0.37535829124378495 1102 22 -1.2244413631016542 1102 23 0.002263369066859866 1102 24 -0.5081260640656993 1102 25 0.16016799603435317 1102 26 -0.02993867837221842 1102 27 -0.04106794304967565 1102 28 -0.12001002872482758 1102 29 0.10871216137922346 1102 30 0.3355937343978326 1102 31 0.6342746493417266 1102 32 0.2455299504847659 1102 33 0.20533340277148682 1102 34 0.03693001155722442 1102 35 0.25402946619884403 1102 36 -0.018787762678172726 1102 37 0.34147172674978776 1102 38 0.0909244733550949 1102 39 0.1042896305615102 1102 40 0.25964983729712543 1102 41 0.2487867266460455 1102 42 0.325797480585841 1102 43 0.44070972748171044 1103 1 -0.06320256675900282 1103 2 0.20150004575459945 1103 3 0.1078495265046584 1103 4 0.434806790663842 1103 5 0.037019978499081674 1103 6 0.7572618075651931 1103 7 0.1277940835080713 1103 8 0.031793561185928354 1103 9 0.19948707921629705 1103 10 0.2677941155590702 1103 11 0.07976517820202389 1103 12 -0.172670101139218 1103 13 0.049308187312902874 1103 14 0.25048855061860564 1103 15 0.3594053603777958 1103 16 0.12729669028334456 1103 17 0.4952795729398929 1103 18 -0.19606667969934222 1103 19 -0.5002804621302914 1103 20 0.5726563775565197 1103 21 -0.04979482084851128 1103 22 0.029297420547401032 1103 23 0.3104103566754617 1103 24 -0.6349598484269023 1103 25 0.13488669707409429 1103 26 -0.0798603419529087 1103 27 0.11581854035724604 1103 28 0.38055969068836504 1103 29 0.15229569648818142 1103 30 0.18340743614971444 1103 31 0.10080363381687363 1103 32 -0.1423065400436485 1103 33 -0.006459789081532108 1103 34 0.30612243428902985 1103 35 0.1857343515428127 1103 36 0.2850863293315129 1103 37 0.13900769028774107 1103 38 -0.1841075733618993 1103 39 -0.3216468457239188 1103 40 0.10226484940366021 1103 41 -0.17722096456603254 1103 42 -0.22626063079430025 1103 43 0.22421635046988797 1104 1 0.5662296939501559 1104 2 0.1217705929978905 1104 3 0.18429961873667366 1104 4 0.6680513079829637 1104 5 0.5151917350065602 1104 6 0.5568792155039759 1104 7 0.5537693516641156 1104 8 0.5158408386334941 1104 9 0.7059433202492897 1104 10 0.5687437637110122 1104 11 0.33767321946731244 1104 12 0.48682296437967665 1104 13 0.48546313709769606 1104 14 0.11746948933555501 1104 15 0.3710880822470308 1104 16 0.4223024974842744 1104 17 -0.17234357387981447 1104 18 -0.4843088058180811 1104 19 -0.476710058359032 1104 20 0.14538302919278578 1104 21 -0.2259921290400797 1104 22 0.36884787487547044 1104 23 0.06199879692961474 1104 24 -0.3086634762194862 1104 25 0.26551455797455503 1104 26 0.0456435181277115 1104 27 0.051187810016330104 1104 28 0.15510365461540232 1104 29 0.14881137593069127 1104 30 0.41241611279110657 1104 31 0.3869961596076763 1104 32 0.36941034183104904 1104 33 0.2864964177914615 1104 34 0.5261128694874984 1104 35 0.3057726107489316 1104 36 0.4023917881003811 1104 37 0.3132920381732376 1104 38 0.7117044099680214 1104 39 0.10077578186369607 1104 40 -0.04639949438263358 1104 41 0.07419667566079369 1104 42 0.30567897431603086 1104 43 -0.029319210095806625 1105 1 0.2735467671208909 1105 2 0.007949454786505328 1105 3 0.33877116345212244 1105 4 0.3591082185520662 1105 5 0.32006978378704787 1105 6 0.49996864639828686 1105 7 0.4887287012576138 1105 8 -0.0939152589274812 1105 9 0.16122787309482334 1105 10 0.2354104303776765 1105 11 -0.08509100817496318 1105 12 -0.017242076270733264 1105 13 0.2090403728700565 1105 14 0.11746948933555501 1105 15 0.24100678143402376 1105 16 0.25970087146801646 1105 17 0.3561117106730336 1105 18 0.12544729174289415 1105 19 -0.062075912017569124 1105 20 -0.4643730683681895 1105 21 -0.3885937550563412 1105 22 -0.7124529381326568 1105 23 0.07825895953124018 1105 24 -0.520045590040624 1105 25 -0.11659926316365699 1105 26 0.1269443311358387 1105 27 -0.12767397860155683 1105 28 -0.15383943481549522 1105 29 -0.11135122569532285 1105 30 -0.05912860265604536 1105 31 0.10244331407922402 1105 32 0.0604672524001586 1105 33 -0.22569870415975402 1105 34 -0.2950253418946147 1105 35 -0.2633330803079801 1105 36 -0.23988463466384502 1105 37 -0.05256162036334899 1105 38 0.004387336797286423 1105 39 -0.2732079579736961 1105 40 -0.20900112039889507 1105 41 -0.47864885279449254 1105 42 0.04551637269001674 1105 43 -0.1024899418031211 1106 1 0.5572240654323366 1106 2 0.4844347581010737 1106 3 0.7548437087929614 1106 4 0.907075698226862 1106 5 0.9138784141810419 1106 6 0.6916509478366848 1106 7 0.4226874254602393 1106 8 0.7585550350062249 1106 9 0.7614780294425358 1106 10 0.618149642385184 1106 11 0.5988989855711253 1106 12 0.5259724327974382 1106 13 0.5303036624260287 1106 14 -0.18215527614286842 1106 15 -0.16981248060475096 1106 16 -0.3012747382880825 1106 17 -0.15720911484291378 1106 18 -0.6095120578506013 1106 19 -0.4408751615485258 1106 20 -0.9967683153975813 1106 21 -0.6486312785084962 1106 22 -0.8295261088643642 1106 23 -0.4051681824324902 1106 24 -0.15519255689490308 1106 25 -0.7021527340829863 1106 26 -0.3706166444739125 1106 27 -0.5331148791644083 1106 28 -0.521444187786102 1106 29 -0.6125394683469807 1106 30 -0.48226806481989826 1106 31 -0.17954542888512748 1106 32 -0.05316626855043438 1106 33 0.21732818764762385 1106 34 0.08621293202659075 1106 35 0.3269108221310475 1106 36 0.3114599557051392 1106 37 0.3405590800744278 1106 38 0.4774955294176735 1106 39 0.5178489525954078 1106 40 0.6769901241289347 1106 41 0.5000253185626065 1106 42 0.24626684173316704 1106 43 0.2933824784595416 1107 1 0.2960767381833094 1107 2 0.296429542977215 1107 3 0.15784240501199065 1107 4 0.41216044398957763 1107 5 0.6602930165278522 1107 6 0.4810903220218208 1107 7 0.5443301894758861 1107 8 0.7848914104951419 1107 9 0.20945716894710387 1107 10 0.433012745383877 1107 11 0.5231493115163701 1107 12 0.5262331889903962 1107 13 0.3170050965850919 1107 14 0.3254066534529585 1107 15 0.18898804090763477 1107 16 0.003810007474630339 1107 17 -2.3388668124368905E-4 1107 18 0.1142167557087177 1107 19 0.4914586063784654 1107 20 0.5695941357106307 1107 21 0.1973921895488715 1107 22 0.029795925033937465 1107 23 0.13785658940584256 1107 24 -0.08439725050637747 1107 25 0.024574775695370477 1107 26 -0.39434545803323573 1107 27 -0.04954251932085896 1107 28 0.007108615342971802 1107 29 0.5324409749385453 1107 30 0.2677971242283377 1107 31 0.4139356662908824 1107 32 0.533665543705105 1107 33 0.5358418773477567 1107 34 0.240319842065702 1107 35 0.19470743230053955 1107 36 0.40494105088114907 1107 37 0.39231918437690183 1107 38 0.2265176936940776 1107 39 0.6212387831038839 1107 40 0.3274464474666132 1107 41 0.33353248935791413 1107 42 0.18172968397567502 1107 43 0.3390148122274681 1108 1 0.3898394500477167 1108 2 0.3438193734856938 1108 3 0.16068986263911 1108 4 0.49107569822686514 1108 5 0.23480149110412185 1108 6 0.6654971016828384 1108 7 0.6005335793063935 1108 8 0.6462473426985298 1108 9 0.3190164909809994 1108 10 0.2439957962313386 1108 11 4.3744710959003896E-4 1108 12 -0.09125833643333436 1108 13 0.23707289319526126 1108 14 0.2661524161648252 1108 15 0.348649057856786 1108 16 -0.05327473828808138 1108 17 0.6938678082340104 1108 18 0.5184879421493989 1108 19 0.5225094538360935 1108 20 0.6460009153716513 1108 21 0.1021379522607333 1108 22 0.19447389113562963 1108 23 0.911754894490592 1108 24 0.38265359695125056 1108 25 0.7036934197631695 1108 26 0.5333833555260838 1108 27 0.32442358237405244 1108 28 0.4477865814446673 1108 29 0.4391528393453257 1108 30 0.5134242428724036 1108 31 0.6712238018841035 1108 32 0.3249875776034088 1108 33 0.2739435722630077 1108 34 0.2948283166419685 1108 35 0.3189108221310448 1108 36 0.49422918647436376 1108 37 0.4162513877667351 1108 38 0.2501109140330584 1108 39 0.2898489525954062 1108 40 -0.22116372202491164 1108 41 0.20187147240876158 1108 42 -0.06511777365144411 1108 43 0.2779978630749298 1109 1 0.4680689582444373 1109 2 0.49162265217421464 1109 3 0.5529521577210801 1109 4 0.8119937310137502 1109 5 0.6055555894647782 1109 6 0.5012348066008698 1109 7 0.6322712842244229 1109 8 0.34657521155099147 1109 9 0.3918689499973951 1109 10 0.23101218967396164 1109 11 0.4899456438309002 1109 12 0.6928072373371563 1109 13 0.2889417456542773 1109 14 -0.12263446908107767 1109 15 -0.09692471263501545 1109 16 -0.3176681809110349 1109 17 0.4002940377422064 1109 18 -0.4980366480145406 1109 19 -0.7140151363278449 1109 20 0.22908288258476261 1109 21 -0.19327188380484017 1109 22 0.17539192392251834 1109 23 -0.14791723665695145 1109 24 -0.06855951780284641 1109 25 0.09431637058284537 1109 26 -0.1509445133263725 1109 27 0.07924325450520087 1109 28 -0.11778718904713514 1109 29 -0.4999291278677873 1109 30 -0.25818231450464424 1109 31 -0.17664505057491908 1109 32 0.052003971046033826 1109 33 -0.08002364085174207 1109 34 -0.11769627352196466 1109 35 -0.06981048934436274 1109 36 0.1996062356546915 1109 37 -0.07961746469228359 1109 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-0.17461805619940662 2216 33 -0.18115501928628674 2216 34 -0.11227027490732411 2216 35 -0.02801875533374698 2216 36 -0.3436863064833773 2216 37 -0.3151288939234078 2216 38 -0.06042429723454745 2216 39 -0.32158766712290543 2216 40 -0.1100087924474451 2216 41 -0.10810035857715405 2216 42 -0.08742763280637433 2216 43 0.12870208842704045 >>END_MODULE >>Per sequence quality scores pass #Quality Count 25 3.0 26 16.0 27 55.0 28 195.0 29 341.0 30 746.0 31 1203.0 32 2061.0 33 3584.0 34 6476.0 35 11735.0 36 17019.0 37 16994.0 38 18554.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.282494745638246 1.1230406928160848 62.795320452761395 1.7991441087842799 2 0.3633739333012585 2.0903496999316302 1.4902129599149172 96.0560634068522 3 63.34607885340964 1.166088475855258 34.39897698209718 1.0888556886379175 4 0.29373781367906615 96.79673849737915 1.8839735635967687 1.0255501253450154 5 0.5330328429262364 0.5203717302676559 0.5178395077359398 98.42875591907017 6 0.44820338811374744 97.49309969360107 0.8267706566053026 1.2319262616798765 7 96.22698842774304 0.7153528652097946 0.6127978526752931 2.4448608543718824 8 1.215497594327678 1.5016459863256522 95.59382122056218 1.6890351987845023 9 24.330518239025565 23.67084921308196 24.47992504336596 27.518707504526517 10 30.867792661619102 21.03897090476311 20.995923121723937 27.09731331189385 11 27.052179748534382 19.23977866973929 21.044101447256796 32.66394013446953 12 25.291212965307675 26.31172448721195 22.89313750316536 25.503925044315018 13 19.390494036615937 15.480742447646298 46.830923501557315 18.297840014180448 14 43.01399354144241 23.661115684163867 15.708225163046919 17.6166656113468 15 26.830503538825795 29.8654106683886 24.189361998455325 19.11472379433028 16 39.99746771334515 24.934160546973917 19.156748543935173 15.911623195745758 17 38.57967638196045 25.71092147577929 22.356992732521334 13.352409409738927 18 18.81995441884021 24.251709293492024 16.7042289187136 40.224107368954165 19 18.790127521622956 24.990185773804246 16.850076614281914 39.36961009029088 20 25.40359851602365 26.847056738037654 24.94270483811743 22.806639907821264 21 23.923172368387736 27.643007267478666 25.158896963865185 23.274923400268417 22 22.311412726950444 23.361018966346762 24.95378693879618 29.37378136790661 23 28.308981793319997 23.426856752171382 24.718290243346587 23.545871211162037 24 25.767896482742902 27.353067787597173 22.958395583803902 23.920640145856016 25 23.40153452685422 23.533210098503456 28.069686764072827 24.995568610569496 26 23.004266848988998 24.103265342297515 27.5002848786417 25.392182930071787 27 25.121231688633976 25.011078613843836 25.918891885390156 23.94879781213203 28 25.985667620470487 24.717024132080727 27.870907295333115 21.42640095211567 29 24.161201286369046 24.89807804309843 27.825327289762225 23.115393380770303 30 26.375629890354762 23.30277784811729 27.89749563191613 22.42409662961181 31 24.403028538147932 24.195386290547212 27.613886708363932 23.787698462940924 32 23.173927906711743 23.901951102163814 28.523315734163912 24.400805256960535 33 22.996378921779645 25.31209642703401 27.398647793168063 24.292876858018282 34 22.61274720822466 25.227266972221518 27.655668380137243 24.504317439416575 35 22.412701628219086 29.38517636929933 26.940315514927455 21.26180648755413 36 23.66741789268441 27.531589476083155 26.542756577448024 22.258236053784405 37 22.739934160546973 26.966320587490504 26.413015953405928 23.880729298556595 38 24.529639664733736 26.54782102251146 27.08591831050113 21.83662100225368 39 25.032285837279378 24.843635258666534 29.848573092603374 20.27550581145071 40 25.37067753903035 24.532458817123974 28.677969535434368 21.41889410841131 41 23.704069487705436 24.06239712324951 31.19856162475626 21.03497176428879 42 23.924438479653592 25.53113367602745 29.159808563976604 21.384619280342356 43 22.568310162323684 28.390184608371953 27.44561776697308 21.59588746233128 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 2.0 8 7.0 9 12.0 10 57.5 11 103.0 12 103.0 13 112.5 14 122.0 15 194.5 16 267.0 17 413.0 18 559.0 19 559.0 20 568.0 21 577.0 22 667.5 23 758.0 24 930.0 25 1102.0 26 1102.0 27 1215.0 28 1328.0 29 1503.0 30 1678.0 31 1733.0 32 1788.0 33 1788.0 34 2090.5 35 2393.0 36 2532.5 37 2672.0 38 3025.5 39 3379.0 40 3379.0 41 3453.0 42 3527.0 43 3957.0 44 4387.0 45 5301.0 46 6215.0 47 6215.0 48 6277.0 49 6339.0 50 6824.0 51 7309.0 52 7410.5 53 7512.0 54 7512.0 55 7267.0 56 7022.0 57 6076.5 58 5131.0 59 4960.0 60 4789.0 61 4789.0 62 3885.0 63 2981.0 64 2516.0 65 2051.0 66 1562.0 67 1073.0 68 1073.0 69 1712.5 70 2352.0 71 1524.5 72 697.0 73 524.0 74 351.0 75 351.0 76 310.0 77 269.0 78 201.0 79 133.0 80 113.0 81 93.0 82 93.0 83 51.5 84 10.0 85 5.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.002532222531716087 9 0.0037983337975741305 10 0.0 11 0.0063305563292902186 12 0.002532222531716087 13 0.0 14 0.02152389151958674 15 0.0012661112658580436 16 0.002532222531716087 17 0.0 18 0.002532222531716087 19 0.018991668987870653 20 0.0063305563292902186 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0012661112658580436 27 0.0012661112658580436 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0012661112658580436 33 0.0 34 0.0 35 0.0 36 0.0 37 0.002532222531716087 38 0.0 39 0.0 40 0.0063305563292902186 41 0.005064445063432174 42 0.0 43 0.005064445063432174 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 78982.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.71930313235927 #Duplication Level Percentage of deduplicated Percentage of total 1 56.57865947049814 19.643716289787548 2 15.210414995259281 10.5619001797878 3 8.733863321420756 9.097009445190043 4 5.714389905914959 7.935985414398217 5 3.836335788782729 6.659745258413309 6 2.629275763985121 5.477197336101897 7 1.8561738749908832 4.511154440252209 8 1.12318576325578 3.119698159074219 9 0.7767485960177959 2.42713529664987 >10 3.318503391437532 19.950115216125194 >50 0.15316169498942456 3.9907827099845536 >100 0.05834731237692363 3.386847636170267 >500 0.007293414047115454 1.4294396191537313 >1k 0.003646707023557727 1.8092729989111445 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TCGACAGTAGCTGTCGGCGATCGCGGCGCGCCGCAGCAGGTGA 1429 1.8092729989111445 No Hit TCGACAGTTTGGTCAGTCCGACGATCGCTTATCGACAGTTTTT 592 0.7495378693879619 No Hit TCGACAGTACCGGTGGGCCAGGCGCAGTGGCTCACACCTGTAA 537 0.6799017497657693 No Hit GCTACAGTTCGCTGATGGTGTGCCCATGCCTGACAAGTACTCC 358 0.45326783317717956 No Hit GCTACAGTCCATTTTGTAAGGGCGCACGGTGGATGCCTTGGCA 258 0.32665670659137525 No Hit GCTACAGTATGCTAATCAACCAGCCAGGTCTCTCCATGTCGTC 213 0.2696816996277633 No Hit GCTACAGTACTTTGCCATCGCCGGCCAGCCCTTCAACCTGGGA 198 0.25069003063989265 No Hit TCGACAGTCGCATTCTGGCTAGGCCAGCTCAGGGCAGGGGGGT 195 0.24689169684231851 No Hit GCTACAGTCCTCTGTCTAGGGGAAATGGTTGGAAACAATAATA 181 0.2291661391203059 No Hit TTGACAGTTCGCTCAGAAGTGGTCCTGCAACTTTATCCGCCTC 147 0.1861183560811324 No Hit TCGACAGTCCGATGTGGCCATCAGGTGCTGCATCTGAGGGTCG 146 0.18485224481527437 No Hit GCTACAGTCATGTGTGAAGAGTTTAAAGAGGCTTTAATCGGTA 142 0.17978779975184217 No Hit CCGACAGTCGCCCGCAGTTTTTTTTTTTTTTTTTTTTTTTTTT 136 0.17219113215669393 No Hit TCGACAGTGTAAAGACAGTTTTTTTTTTTTTTTTTTTTTTTTT 132 0.16712668709326176 No Hit GCTACAGTGTCTTGTTCTACAGTCCGATGATCTCGAGTCGAGC 124 0.1569977969663974 No Hit TAGCCAGTGCGCCGACGAACGTCGGCGCTTCCATATCCGTTTC 118 0.14940112937124916 No Hit TCGACAGTAGCTGTTCGGCGATCGCGGCGCGCCGCAGCAGGTG 113 0.14307057304195892 No Hit TCGATAGTTTTGCGTAAAAGTGTTTCTATTTCTCCACCATTTG 112 0.14180446177610087 No Hit TCGACAGTACACCGAGGTCTCTGGGGACCTTCTGAGGTGGAGG 102 0.12914334911752046 No Hit GCCACAGTCTGATGCGTCCCGGCAAGTTCAGGTTCGTCTACAC 99 0.12534501531994632 No Hit GCTACAGTCGTATGGGGCGCCATGCTGAGGGGAAGGGAAGATG 96 0.12154668152237218 No Hit GCTACAGTTGGGTAAGTCCAATCACCAGGAATTTGAGGCAGTG 96 0.12154668152237218 No Hit CCAACAGTAGCTAAAAAGTGCTTGGATGAGGTGTGGGTAGCGG 95 0.12028057025651415 No Hit TCGACAGTAGCTCAAAGGCCAAAGATAAAAAGATTTTTTTTTT 95 0.12028057025651415 No Hit GCTACAGTCCGCTATTCTTGGACCGGCGCAAGACGGACCAGAG 94 0.1190144589906561 No Hit GCTACAGTGTGAGAAACGCCCTGCTGTGTTGCAGCAACGGCAT 93 0.11774834772479804 No Hit TCGACAGTGGCACGAAGGATGTGTGTCTCTGCACACACTGAAG 92 0.11648223645894001 No Hit GCTACAGTCCTCTGGTCTAGGGGAAATGGTTGGAAACAATAAT 91 0.11521612519308196 No Hit TCGACAGTAGCTGGTCGGCGATCGCGGCGCGCCGCAGCAGGTG 91 0.11521612519308196 No Hit GCTACAGTCTCCTGTGGACTCTAAGTAAGGGTAAAATGGGAGA 88 0.11141779139550784 No Hit TCGAGACTAGTCTAAACAGCAACAACAAAAAACTACCCATCAG 85 0.1076194575979337 No Hit TCGACAGTGCACACAGTCCGACGATCACAGTTCGACAGTAGAA 84 0.10635334633207567 No Hit TCGACAGTCAGAGGGAGAAATGACCGCCTTTCTTACAACCAAA 83 0.10508723506621762 No Hit TCGACAGTCATGAGAAGAAACCCAAGTTTGAATTGGGCAAGCT 81 0.10255501253450153 No Hit GCTACAGTTCGCTGGATGGTGTGCCCATGCCTGACAAGTACTC 81 0.10255501253450153 No Hit TCGACAGTCCAACGAGGCCAGAACCCACTGCCTCTGGGCCCTC 80 0.1012889012686435 No Hit TCGACAGTTGCAATGAGGATCTGCTTTGACTCAGGTTGGTGAT 79 0.10002279000278544 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.005064445063432174 0.0 0.0 14 0.0 0.0 0.007596667595148262 0.0 0.0 15 0.0 0.0 0.007596667595148262 0.0 0.0 16 0.0 0.0 0.010128890126864349 0.0 0.0 17 0.0 0.0 0.010128890126864349 0.0 0.0 18 0.0 0.0 0.011395001392722393 0.0 0.0 19 0.0 0.0 0.01392722392443848 0.0 0.0 20 0.0 0.0 0.02152389151958674 0.0 0.0 21 0.0 0.0 0.030386670380593048 0.0 0.0 22 0.0 0.0 0.03924944924159935 0.0 0.0 23 0.0 0.0 0.4621306120381859 0.0 0.0 24 0.0 0.0 0.6077334076118609 0.0 0.0 25 0.0 0.0 0.6317895216631637 0.0 0.0 26 0.0 0.0 0.8305689904028766 0.0 0.0 27 0.0 0.0 0.9799701197741257 0.0 0.0 28 0.0 0.0 1.1040490238282141 0.0 0.0 29 0.0 0.0 1.2040718138309994 0.0 0.0 30 0.0 0.0 1.3522068319363905 0.0 0.0 31 0.0 0.0 1.4648907345977564 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACTCAT 105 0.0 37.000004 5 TTGGTCA 105 0.0 37.000004 9 TCCGGCC 20 0.0018315739 37.0 13 GTGTTTC 25 1.2232414E-4 37.0 20 ACAACGG 20 0.0018315739 37.0 9 GTCATGT 25 1.2232414E-4 37.0 7 GTTTGAT 65 0.0 37.0 7 GGAATGC 20 0.0018315739 37.0 9 CTGTCTA 25 1.2232414E-4 37.0 12 GTCGCCT 30 8.214271E-6 37.0 7 ACCGGTG 50 1.7462298E-10 37.0 9 AGTCCCG 20 0.0018315739 37.0 6 ACAGTAT 325 0.0 37.0 4 AGTCATC 25 1.2232414E-4 37.0 6 CGTATGG 20 0.0018315739 37.0 9 GTATTAG 45 2.5538611E-9 37.0 7 GACACTA 20 0.0018315739 37.0 3 GGGTCCG 20 0.0018315739 37.0 12 TTTAACC 20 0.0018315739 37.0 24 ATGTGGC 25 1.2232414E-4 37.0 12 >>END_MODULE