FastQCFastQC Report
Thu 26 May 2016
SRR2099139_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099139_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81126
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTAGTCCTAGGGCCAGGAGGAGCCGACTAGGAGTCCTGGCT4280.527574390454355No Hit
AGCTTGGCCGACCGCTGGCAGGCGGAAGGCAGGATCCCGAGGC3300.40677464684564746No Hit
AGCTAGTCCCGTCGGTGCCCAGCCGCTGCCTGCTCTGTTATCT3300.40677464684564746No Hit
AGCTAGTCTCCGAAGGTTGGCGCTTGCCGCTATAATGCGCATT3240.39937874417572666No Hit
AGCCAGTCCGTGCCACGTGTCCCGATGACCAGCAGGGCCACGA2900.35746862904617505No Hit
AGCTGCTCGTACTAGCAGCTAGTACGATCGTCGGACTGTAGAA2760.34021152281635975No Hit
AGCTAGTCTCGGCCCGGCATCACGCGCTCTTGGACGTGGACAG2740.33774622192638615No Hit
AGCTAATTTTTACGTGAGGAAAGAGGGCGTCAGCATTCGATAC2590.31925646525158397No Hit
AGCTAGTCGCCAGGGCTGTGCATCCGTCTTTCACAAGGTCACC2520.3106279121366763No Hit
AGCTAGTCGCCATGAGACCACCACCCTGCAAGGCTCCTGGTCC2480.30569731035672904No Hit
AGCTAGTCCATGCGTGGCTTCAAAGAAGCTATTGATTCTGAAG2210.27241574834208515No Hit
AGCTAGTCACCTTAAGACCCCAGAGGCCAACCTCGGCGGGGTG2000.24653008899736215No Hit
AGCTAATTTTTACGGTGAGGAAAGAGGGCGTCAGCATTCGATA1840.22680768187757316No Hit
AGCTAGTCAAACCGGAACGGACACTGTCCCAGAGAGAAGTCGG1680.20708527475778418No Hit
AGCTAGTCAACAGGGCGAAGCAAAATGGCAGATCGATGGAGTC1640.20215467297783696No Hit
AGCTAGTCTCTCGGGGCAGGGCAAAAAGGCGTGTGAACGGCAG1480.18243226585804798No Hit
AGCTAGTCAAAGGGCAGACTGGCGCGCCCCAGGGGTCTCTGGG1420.1750363631881271No Hit
AGCTAGTCTCTCGGGGGCAGGGCAAAAAGGCGTGTGAACGGCA1270.15654660651332494No Hit
AGCTAGTCTCGGCGGCCGGCATCACGCGCTCTTGGACGTGGAC1260.15531395606833814No Hit
AGATGGTCCGCCCGCGCCGCCACAACTGCCTGTCCTTCGTGCT1180.14545275250844367No Hit
AGCTAGTCGCCCCGTGGGCGGCCTGAGCCAGTTCCAGTGGATG1100.13559154894854916No Hit
AGCTAGTCACCTCGCGGACGATGATCGGCTCAACAAGGTCATC1080.13312624805857556No Hit
AGCTAGTCAGCCATCCTTCTATCCTTCTCAGATCAGGTTGCTG1060.13066094716860194No Hit
AGCTAGTCGGCCTTGTGCCAAGCCTCCCTCCCTGGGCTACAGG1010.12449769494366786No Hit
AGATGGTCCGCCCCGCCGCCACAACTGCCTGTCCTTCGTGCTG1010.12449769494366786No Hit
AGCTTGCCGGTTGCGAAGGGGTGACCGGCCAGATCCAGGGCAG1000.12326504449868107No Hit
AGCTAGTCCTAGGGGCCAGGAGGAGCCGACTAGGAGTCCTGGC980.12079974360870743No Hit
AGCTGGTTACACGCTTAGTTTTGCAATGAAGTGTTATTAATCA970.11956709316372065No Hit
AGCTAGTCACATGGACGATCCGGCTAGCTAGTCTTTTTTTTTT950.11710179227374701No Hit
AGCCAGTCCCGGCACGAGACCAAGCGCTGGTTCGCTGGCGTGG930.1146364913837734No Hit
AGCAGGTCATTGGGCAGGCACTGAATTTTACTGAAGACACGCT920.11340384093878658No Hit
AGCTAGTCTATGCCAACAACTTCGTGCTGGTGCAGCTGCTGAC840.10354263737889209No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAGC200.001827368937.0185119
TAGCCTA251.2199342E-437.0185115
GCCTAGA200.001827368937.0185117
GCAGACC200.001827368937.0185113
GGGGCCT200.001827368937.0185114
GCGAGAC200.001832917836.99568612
TCACGCC251.2243765E-436.9956867
GTCATTT251.2243765E-436.9956866
TCGCCCC200.001832917836.9956867
CTGCTCG1300.036.9956863
GTCATGA200.001832917836.9956866
GTCATAG251.2243765E-436.9956866
GTCATAC251.2243765E-436.9956866
GTCGCTT200.001832917836.9956866
TCTGAGT251.2243765E-436.99568623
GTCGCGA251.2243765E-436.9956866
TCCATGA403.7625796E-836.9956867
AGTCCGT950.036.9956865
AGTCCGG1200.036.9956865
AGTCCGC950.036.9956865
Produced by FastQC (version 0.11.5)