FastQCFastQC Report
Thu 26 May 2016
SRR2099138_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099138_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166183
Sequences flagged as poor quality0
Sequence length42
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGCTGGGAGAGCCGCGCCTTGTTCGGTCCGCTGGCAACGC7760.4669551037109692No Hit
ACTGCTGCCTTTGGTACTCAGGCACTGCAGCAAGAGCTGGGC7500.45130970075158106No Hit
ACTGCCCGCGTGTAGTCCTGCACCTCAGGATTAGAGAGGTCG6770.4073822232117605No Hit
ACAGCTAGCAGTGCGGCGCGTGCGCGGGCGGGGCCCGCGTGC6410.38571935757568465No Hit
ACTGCTAGCCCAGTGCAATTTATTGTTGTTTCTCCAAAATCA5460.32855346214715103No Hit
ACTGCTATGGGACTAGTTTTTTTTTTTTTTTTTTTTTTTTTT5080.30568710397573756No Hit
ACTGCTAGGAGGGGGTTGCCACGAATCTTCAGGTTTTAAAAA4860.2924486860870245No Hit
ACTGCTAGCATGCATGAGAAGATAGCAAGTGTGCCAACTAAG4840.291245193551687No Hit
ACTGCATGCGACTTGCCTTTGCGTAACGAAACGCGCAGCATC4490.2701840741832799No Hit
ACTGCTAGAGCTGGAACTGTGGCACCTGCTGGACAGCGTGCC3920.2358845369261597No Hit
ACTGCTGGGAGAGGCCGCGCCTTGTTCGGTCCGCTGGCAACG3470.20880595488106485No Hit
CCTGCTGGCGGGATGGTGTCGTGCGGCAGCACATTGATGGGC3330.200381507133702No Hit
ACTGCATGCGACGCCTCCGGCCTTTGAGCATTGCCTTTGCGT3310.19917801459836448No Hit
CCTGCTGGCGGGACGATGGCCAGGGCCATCAGGGGCTGGCCG3300.1985762683306957No Hit
ACTGCTAGTTAGTCCCACTTTTTCATTAACGTTGACGCCCCT3280.1973727757953581No Hit
ATTGCTATCATGGTCCGACGATCCATGGACGGTAGTTCAGTG3180.19135531311867038No Hit
ACTGCTAGTTCGTACAGTCCGACGATCAAATACTGCTAGTTT3170.19075356685100162No Hit
ACTGCTAGTAAGGGAGGGTAAGATGAAATTATAAGATGTCTT3020.18172737283596999No Hit
ACTGCATGCGACGGCCTCCGGCCTTTGAGCATTGCCTTTGCG2970.17871864149762612No Hit
ACTGCTAGCAGTGGTTCTTTGCAGAACAGATGCTAACTAACA2930.176311656426951No Hit
ACTACTAGACGGCCGACGATCTTGGACTGCAGAACTCTGAAC2900.17450641762394467No Hit
ACTGGGAGAGTCGAGTAGCTTGCACTGATCGTCGGACTGTAG2840.17089594001793204No Hit
ACTGCTAGCTATGTCGACGATCGATGACTGCAGAACTCTGAA2780.16728546241191938No Hit
ACTGCTAGGCAAGAAGGAGACACAGAGCCCAGACCTCCTTTG2750.16548022360891307No Hit
CCTGCTGGCCGAGCTCGACCGCGGCGGCGAGACGCTCTTCGA2750.16548022360891307No Hit
ACTGCTAGGTAACCGACGATCTTGGACTGCAGAACTCTGAAC2690.1618697460029004No Hit
ACTGCTGCCTTTGGGTACTCAGGCACTGCAGCAAGAGCTGGG2560.15404704452320633No Hit
ACTGCTAGCCCAGGTGCAATTTATTGTTGTTTCTCCAAAATC2510.1510383131848625No Hit
ATCGCTAGCATGCTAATGCCTACATTGTAGGCATTAGCAAAT2430.14622434304351226No Hit
ACTGCTAGTTTCGAGGCCCCAGAGAGTGGGGAGGGTAGAGGT2380.14321561170516842No Hit
ATTGCTAGTCGTGATCCGACTATCATCTTCTTTTTCACTCAC2370.14261386543749963No Hit
ACTGCTAGTTAGGTCCCACTTTTTCATTAACGTTGACGCCCC2340.14080862663449328No Hit
ACTGCTAGGGAGCATAAACTGCTAGTTTTTGATCGTCGGATT2260.1359946564931431No Hit
ACTACTAGGTTCCAGTCCGACGATCTCGGACTGTAGAACTCT2180.1311806863517929No Hit
ACTGCTAGCAGTTCTGTTCTTTGCAGAACAGATGCTAACTAA2120.12757020874578026No Hit
ACTACGAGTCCATGGTAGTCGCCGTGCCTACCATGGTGACCA2050.12335798487209883No Hit
ACTGCTGATGGTGACAGGGCCACTCCCACGACCAACACGGCG2040.12275623860443007No Hit
ATTGCTACAGCTCTTCACTAACAATATTTCCAACCAACAATA2030.12215449233676128No Hit
ACTGCATGCGACGGCCGCCGGCCTTTGAGCATTGCCTTTGCG1860.1119248057863921No Hit
ACTGCTAGCAGTGAGGCTGTCTCTGACTGCTGCCTGTCTGGT1830.11011956698338578No Hit
ACGGCTCGTCAGCCGGTGGCCCACAGGAGAGCACAGGCCCAG1810.10891607444804823No Hit
ACTTCTAGGTACCAGGATCAACAGGAAGAGGAGCCAATTCCT1790.10771258191271069No Hit
ACTGCTAAAGCCCAGTCCGACGGACTGTAGAACTCTGAACCC1690.10169511923602294Illumina DpnII expression Sequencing Primer (95% over 24bp)
ACTACTAGTCGTTGTGAATTTCCAGGGAAACTAACATTGATA1680.10109337296835416No Hit
ACTGCTGATGGTGGACAGGGCCACTCCCACGACCAACACGGC1670.1004916267006854No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTCGG200.002051049636.18008836
ATAAAAC356.665214E-736.18008436
AGGTAAC453.3614924E-936.0274287
TCGCATG309.926225E-636.0274286
GGTACGG200.00209408536.0274288
GCGACCG601.8189894E-1236.0274288
GCGACAT251.4375994E-436.0274288
GAGCTAT601.8189894E-1236.0274288
TAGACTA200.00209408536.0274286
TAGACGG601.8189894E-1236.0274286
TAGACGC200.00209408536.0274286
TAGACAC309.926225E-636.0274286
TAGGTTA200.00209408536.0274286
TAGGTCT1000.036.0274286
CATGAAT200.00209408536.0274289
TAGAAAG404.803769E-836.0274286
TAGAAAC251.4375994E-436.0274286
TAGCGCG453.3614924E-936.0274286
TAGCGCA601.8189894E-1236.0274286
GAATCTC200.00209408536.0274288
Produced by FastQC (version 0.11.5)