##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2099138_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	166183
Sequences flagged as poor quality	0
Sequence length	42
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.98768225390082	35.0	35.0	37.0	30.0	37.0
2	34.74243454504973	37.0	35.0	37.0	31.0	37.0
3	34.69484243273981	37.0	35.0	37.0	31.0	37.0
4	34.54272699373582	37.0	35.0	37.0	30.0	37.0
5	36.545753777462195	39.0	37.0	39.0	32.0	39.0
6	36.323607107826916	39.0	37.0	39.0	31.0	39.0
7	35.14061005036616	37.0	34.0	39.0	29.0	39.0
8	35.818717919402104	38.0	35.0	39.0	30.0	39.0
9	30.443378684943706	31.0	30.0	33.0	27.0	34.0
10	30.82045094865299	31.0	31.0	34.0	27.0	34.0
11	30.927742308178335	31.0	31.0	34.0	27.0	34.0
12	34.536613251656306	37.0	35.0	37.0	30.0	37.0
13	31.16412027704398	31.0	31.0	33.0	30.0	34.0
14	31.10195387013112	31.0	31.0	34.0	28.0	34.0
15	31.04294663112352	31.0	31.0	34.0	27.0	34.0
16	34.24503108019473	35.0	33.0	37.0	30.0	37.0
17	34.70567386555785	35.0	35.0	37.0	32.0	37.0
18	34.5089690281196	35.0	35.0	37.0	31.0	37.0
19	34.17245446285119	35.0	35.0	37.0	30.0	37.0
20	34.26404024479038	35.0	35.0	37.0	30.0	37.0
21	35.782420584536325	37.0	35.0	39.0	30.0	39.0
22	36.01114434087722	37.0	35.0	39.0	31.0	39.0
23	36.37625388878526	37.0	35.0	39.0	32.0	39.0
24	36.574505214131406	38.0	35.0	39.0	32.0	39.0
25	36.68183267843281	38.0	35.0	39.0	32.0	39.0
26	37.93110005235192	39.0	37.0	40.0	33.0	41.0
27	38.040118423665476	39.0	37.0	40.0	33.0	41.0
28	38.17667270418755	40.0	37.0	40.0	34.0	41.0
29	38.24612625840188	40.0	37.0	40.0	34.0	41.0
30	38.27812110745383	40.0	38.0	41.0	34.0	41.0
31	38.41119729454878	40.0	38.0	41.0	34.0	41.0
32	38.430374948099384	40.0	38.0	41.0	34.0	41.0
33	38.43806526540019	40.0	38.0	41.0	34.0	41.0
34	38.42151122557662	40.0	38.0	41.0	34.0	41.0
35	38.39259731741514	40.0	38.0	41.0	34.0	41.0
36	38.346587797789184	40.0	38.0	41.0	34.0	41.0
37	38.301517002340795	40.0	38.0	41.0	34.0	41.0
38	38.27875294103488	40.0	38.0	41.0	34.0	41.0
39	38.24122202632038	40.0	38.0	41.0	34.0	41.0
40	38.18160702358244	40.0	38.0	41.0	34.0	41.0
41	38.003863211038436	40.0	38.0	41.0	34.0	41.0
42	38.0590072390076	40.0	38.0	41.0	34.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.35350748545329935
1101	2	0.48829490702956235
1101	3	0.6918179399490896
1101	4	0.5603242816716261
1101	5	0.6027375361454546
1101	6	0.9955800996565642
1101	7	0.9310546123362826
1101	8	0.7835764061067394
1101	9	0.2671093671875546
1101	10	0.535934827666388
1101	11	0.6931189950898222
1101	12	0.7662096987215747
1101	13	0.4172337868298541
1101	14	0.27507895316489694
1101	15	0.098157214656311
1101	16	0.1963802762655078
1101	17	0.05393274956316674
1101	18	0.08945398967363616
1101	19	0.270578840553803
1101	20	-0.15699390104736466
1101	21	0.36924989351761894
1101	22	0.2008898905720642
1101	23	-0.12564894944658533
1101	24	0.5541876238316945
1101	25	0.2991873239245635
1101	26	0.0030893680595696082
1101	27	-0.09615224535788514
1101	28	0.10698202412320512
1101	29	0.4802270899186496
1101	30	-0.06604167049355425
1101	31	0.11742507616505549
1101	32	0.2687452215506454
1101	33	0.177883184143802
1101	34	0.12047957121005481
1101	35	0.19972149874016765
1101	36	0.2639448137832261
1101	37	0.2321140792823897
1101	38	0.21568148260565323
1101	39	0.2294647778097314
1101	40	0.3959822604418761
1101	41	0.6100112296461901
1101	42	0.29358250299862476
1102	1	0.271468269767027
1102	2	0.38154980899034996
1102	3	0.10703362622359691
1102	4	0.4547556542206479
1102	5	0.4574826341846716
1102	6	0.5848350016173498
1102	7	0.8939565731205974
1102	8	0.9666352296361467
1102	9	0.3272662299326541
1102	10	0.2775818864899158
1102	11	0.5702954656780577
1102	12	0.00715087519216695
1102	13	0.4957435907514238
1102	14	0.604804443360976
1102	15	0.5970591754406271
1102	16	0.7008508645008007
1102	17	0.517854318190615
1102	18	0.5052971269285393
1102	19	0.520931781730269
1102	20	0.4924178636585168
1102	21	0.5273675405764422
1102	22	0.44418400821912485
1102	23	0.17991967800439568
1102	24	0.1715993885375724
1102	25	0.2994226180422075
1102	26	0.262932505314474
1102	27	0.6214948134656453
1102	28	0.6810996711820252
1102	29	0.49316826638923317
1102	30	0.5567818589182139
1102	31	0.48283684087093803
1102	32	0.5022354176290733
1102	33	0.6212557331634088
1102	34	0.70965604179829
1102	35	0.6306234595244788
1102	36	0.3415134412342056
1102	37	0.18646702045886343
1102	38	0.523210894370358
1102	39	0.47079811114306835
1102	40	0.3566881427948161
1102	41	0.5987955433716792
1102	42	0.698680542214305
1103	1	-0.24888331914440442
1103	2	-0.06604992055664383
1103	3	0.14521257596441473
1103	4	0.08896795983255146
1103	5	0.12270688480445813
1103	6	-0.030580819883667232
1103	7	0.7432691717232558
1103	8	0.4529097394400736
1103	9	0.35886415645958536
1103	10	0.090969310425006
1103	11	0.2130270410668338
1103	12	-0.18946846219796498
1103	13	0.361463671887325
1103	14	0.23973795699631495
1103	15	0.336793229981982
1103	16	0.23779023795133014
1103	17	0.19132738557848938
1103	18	0.5051168249226805
1103	19	0.3136746259944161
1103	20	0.08101376178788655
1103	21	0.4363150276172334
1103	22	0.3345603886563566
1103	23	0.20624377085992762
1103	24	0.08969720237575274
1103	25	0.01233674921191863
1103	26	0.1010663795538207
1103	27	0.1069128887417321
1103	28	0.2850663153109423
1103	29	0.3417596569684562
1103	30	0.40677059004284644
1103	31	0.335034271567352
1103	32	0.3200402407077334
1103	33	0.3063582799292419
1103	34	-0.0445472487133145
1103	35	0.16575981291641284
1103	36	0.25596013945372675
1103	37	0.09108725935902129
1103	38	0.3088768849045067
1103	39	0.12443795788636436
1103	40	-0.1390522223167423
1103	41	0.095482494014
1103	42	0.3865326945695031
1104	1	0.20502872097453206
1104	2	0.24043390316855806
1104	3	0.1905978627290139
1104	4	0.364741270088615
1104	5	0.3406834820914
1104	6	0.08773453981100232
1104	7	0.3139580872397616
1104	8	0.003746290276623654
1104	9	-0.3490605169823304
1104	10	-0.24160957387801574
1104	11	0.11020007617090144
1104	12	0.23413247864435505
1104	13	-0.03204806645199909
1104	14	0.19223725432319938
1104	15	0.1390684115675107
1104	16	0.08345363533886285
1104	17	-0.1262216905912723
1104	18	0.130658622878272
1104	19	0.035490037464995794
1104	20	-0.0014726655261299015
1104	21	-0.4153987551310294
1104	22	0.03205591373808403
1104	23	0.06375645595881707
1104	24	0.11400229564636533
1104	25	-0.005414992677749808
1104	26	-0.025991713021511487
1104	27	0.20886705966142216
1104	28	0.20397044111162188
1104	29	0.10316145285300848
1104	30	0.24235987390799352
1104	31	0.15574167848166098
1104	32	0.36052900533442767
1104	33	0.04934264360326068
1104	34	0.29048729321777955
1104	35	0.3286172516359187
1104	36	0.49639269023110444
1104	37	0.5033418785101915
1104	38	0.34060812353229863
1104	39	0.2548392951842544
1104	40	0.2553027237623411
1104	41	0.6326058242407839
1104	42	0.46323501265113265
1105	1	0.5991371150829252
1105	2	0.44059120332585877
1105	3	0.3826327547639039
1105	4	0.6392872446345947
1105	5	0.735478276886198
1105	6	0.8920245441010124
1105	7	0.5427583160399863
1105	8	0.4825393690696984
1105	9	0.08325751533570269
1105	10	0.4228977906293494
1105	11	0.43652640249722907
1105	12	0.48013562464750237
1105	13	0.22864119423726237
1105	14	0.21700487909082256
1105	15	0.060675733174829105
1105	16	0.3540099058951327
1105	17	0.2657846014150138
1105	18	-0.1766200844004402
1105	19	0.053393655368616066
1105	20	0.2667098026563437
1105	21	0.42954618981391235
1105	22	0.3311121127942869
1105	23	-0.10461191240955259
1105	24	0.17552095716502691
1105	25	-0.0554793427421032
1105	26	0.0847189976892011
1105	27	0.14458849538285534
1105	28	0.00920424634542627
1105	29	0.28096783065938524
1105	30	-0.1464861149379928
1105	31	-0.05398233124235219
1105	32	0.12489336969879616
1105	33	-0.26567237141175326
1105	34	-0.13152042878994763
1105	35	0.049795572814240074
1105	36	0.4347596285980444
1105	37	0.0744844496527648
1105	38	-0.1632814803573055
1105	39	-0.3958685555235988
1105	40	0.05864892710854264
1105	41	0.32690011853507883
1105	42	0.17950842892454943
1106	1	0.041477182422994474
1106	2	0.20262824036289828
1106	3	0.49887854600969916
1106	4	0.5581424634898084
1106	5	0.49322238463030743
1106	6	0.6816710087474718
1106	7	0.4186000668817371
1106	8	0.19912186065219117
1106	9	0.33435179142998095
1106	10	0.6561469488785079
1106	11	0.4344220253928519
1106	12	0.26481575932763946
1106	13	0.3515368171328852
1106	14	0.34562440771034986
1106	15	0.39670266920176545
1106	16	0.6561984580836864
1106	17	0.21544790107831346
1106	18	-0.05178843456878468
1106	19	-0.1727847958098323
1106	20	0.08497579592233251
1106	21	0.327946863214585
1106	22	0.12252625420843088
1106	23	0.14698741418978045
1106	24	0.04067247231654392
1106	25	0.11800550574274382
1106	26	-0.19112275315255545
1106	27	0.1993023000966616
1106	28	0.26425475139593146
1106	29	0.28568163537319435
1106	30	0.1449280264761441
1106	31	0.32236447010444635
1106	32	-0.08992144511601907
1106	33	-0.14875317949256583
1106	34	0.17832805605853963
1106	35	-0.015187592168928177
1106	36	0.2070660259044388
1106	37	0.2533565035248131
1106	38	0.23756027048444395
1106	39	-0.03905037370541464
1106	40	-0.20801773955812308
1106	41	0.2614960781310387
1106	42	0.10130977572589472
1107	1	0.12004511986189925
1107	2	0.11167841957436764
1107	3	0.11728030554048985
1107	4	0.27596585873256174
1107	5	0.36247947162932803
1107	6	0.26765178424437863
1107	7	0.2906675155620917
1107	8	0.5353470154257352
1107	9	-0.013277729586636866
1107	10	0.14559791010366396
1107	11	0.025534765699138262
1107	12	0.1423817417323292
1107	13	0.039871779661396545
1107	14	0.21007536893550594
1107	15	0.0460998669860615
1107	16	0.11147705045905809
1107	17	0.05586823343413272
1107	18	-0.15487575943030407
1107	19	0.11814873302692064
1107	20	0.2612139842572958
1107	21	0.21891297595489334
1107	22	0.14867483680862392
1107	23	0.13505355951398457
1107	24	0.0801804553729113
1107	25	-6.40633064683982E-4
1107	26	-0.2567385889296787
1107	27	-0.05314149266970958
1107	28	0.29594259759990393
1107	29	0.02696544117312527
1107	30	0.29652463775017424
1107	31	0.03729604390699137
1107	32	-0.104258362678749
1107	33	-0.04094118861605267
1107	34	0.2351462378767195
1107	35	-0.02642903889424275
1107	36	-0.010879558976625958
1107	37	0.1091320004293479
1107	38	0.04735173350171351
1107	39	-0.06898683509349013
1107	40	0.16832634646338107
1107	41	0.2889072869938545
1107	42	-0.0894282496895542
1108	1	0.31000927756799257
1108	2	0.12959956652777294
1108	3	0.5258824560781221
1108	4	0.11825976554259654
1108	5	0.25495258990889624
1108	6	0.28557293119778393
1108	7	0.5702374080352115
1108	8	0.34179862832895935
1108	9	0.09424915213379137
1108	10	-0.34185728702895446
1108	11	-0.3615620084944098
1108	12	0.12087636538824142
1108	13	-0.15367660743537925
1108	14	0.24233343345163405
1108	15	-0.11519045559458263
1108	16	-0.15017169506065642
1108	17	0.10963167429434861
1108	18	-0.04376464831919691
1108	19	-0.23310574725982036
1108	20	0.16802402009959394
1108	21	-0.014061934439375534
1108	22	-0.1093896793204081
1108	23	-0.1839428562566212
1108	24	-0.24956864856974192
1108	25	0.16781814829732156
1108	26	-0.4144446821196439
1108	27	-0.2574425679385328
1108	28	-0.2631971873463286
1108	29	-0.2776940570347577
1108	30	-0.026056007411114024
1108	31	-0.1419154256270616
1108	32	-0.5128605132163813
1108	33	-0.24882649327555129
1108	34	-0.2738143355999796
1108	35	-0.4386154188225575
1108	36	-0.43381862707698815
1108	37	-0.009147569463131333
1108	38	-0.19279163567391322
1108	39	-0.09049221143757791
1108	40	-0.14350161052586685
1108	41	-0.11969486354377779
1108	42	0.14713089009539715
1109	1	-0.0061022706442628305
1109	2	0.04568863525256006
1109	3	0.40719076224873874
1109	4	0.2961849088493267
1109	5	0.29369223997820626
1109	6	-0.010956485709286312
1109	7	0.2432357970052763
1109	8	0.11260776499175762
1109	9	0.18319299088093643
1109	10	-0.056093046898073595
1109	11	0.08835244456717106
1109	12	0.2277288624846463
1109	13	-0.24496830376248013
1109	14	0.1454343538617593
1109	15	-0.002093656423827639
1109	16	-0.08746641362996144
1109	17	0.09442055444121422
1109	18	-0.22728468628454834
1109	19	-0.13354659498627086
1109	20	-0.41469424947941746
1109	21	-0.2684922667611289
1109	22	-0.14442021395755233
1109	23	-0.16046428045704175
1109	24	0.041490759720197445
1109	25	0.13348697549250943
1109	26	-0.30379564936203707
1109	27	-0.019497193093073406
1109	28	-0.10151971803707482
1109	29	-0.3085533978862287
1109	30	-0.3647594405283954
1109	31	0.14924389149606299
1109	32	0.09590898461684816
1109	33	0.11253126776749411
1109	34	0.04234716702887198
1109	35	-0.19779766502290386
1109	36	0.048516242354658345
1109	37	-0.05546780225071046
1109	38	0.07947242337220217
1109	39	0.07544387188639234
1109	40	0.06027494336870376
1109	41	0.4909729021200562
1109	42	0.4553246632773664
1110	1	0.010323811983909081
1110	2	-0.027813936507854464
1110	3	-0.045107230118937025
1110	4	-0.371975038056263
1110	5	-0.25152096725590667
1110	6	0.13737601802391453
1110	7	-0.3650270203167807
1110	8	-0.023280736750407982
1110	9	-0.33904029267639046
1110	10	-0.27884068253769456
1110	11	-0.1953708008285453
1110	12	0.11067228375559068
1110	13	-0.4202628118096001
1110	14	-0.4082407747262593
1110	15	-0.6122645540511691
1110	16	-0.4116741455031985
1110	17	-0.743890379688537
1110	18	-0.5943827450202406
1110	19	-0.4335300029836162
1110	20	-0.23359254050314604
1110	21	-0.5218521472987092
1110	22	-0.3346475243939224
1110	23	-0.17498228277992212
1110	24	-0.5237987707261311
1110	25	-0.1990167577080868
1110	26	-0.12518274078396985
1110	27	-0.5451318371946172
1110	28	-0.39669144526455113
1110	29	-0.6237184883126474
1110	30	-0.4517015344391311
1110	31	-0.0724252639709988
1110	32	-0.011690152599015846
1110	33	-0.2026338226589175
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2210	11	0.2513412173120422
2210	12	0.36101973456386816
2210	13	-0.12141854291925114
2210	14	-0.2128636991648527
2210	15	-0.301570383909997
2210	16	-0.006802519433421139
2210	17	0.15622665637319955
2210	18	-0.047348877709872284
2210	19	0.22567561474735243
2210	20	0.2253716903504852
2210	21	0.16873376448535993
2210	22	0.4174920411096963
2210	23	-0.05132636880142627
2210	24	0.5031195234732664
2210	25	0.14989700134391626
2210	26	0.22354814942157475
2210	27	0.24434954675680842
2210	28	0.4464802320085113
2210	29	0.41406221536667687
2210	30	0.47215187789354474
2210	31	0.2595182661292128
2210	32	0.246996117607992
2210	33	0.3640767325308971
2210	34	-0.06951326033116345
2210	35	0.28539891809500517
2210	36	0.1898372869015077
2210	37	0.438881104371994
2210	38	0.24806857937984717
2210	39	0.17832499286349446
2210	40	0.0930575292590845
2210	41	0.4824556740906303
2210	42	0.17938895461152526
2211	1	-0.3638258478800367
2211	2	-0.4890384263037717
2211	3	-1.66551539338424
2211	4	-0.8166581744687207
2211	5	-1.756139656837
2211	6	-1.2225953389399251
2211	7	-0.7749804753830176
2211	8	-0.8804235938932621
2211	9	-0.46138186088262145
2211	10	-0.8611178039125598
2211	11	-1.149828373331232
2211	12	-0.5738604767170159
2211	13	-0.8033276166789172
2211	14	-1.0668157836772103
2211	15	-0.4788954169226365
2211	16	-1.5352688465415127
2211	17	-0.39483918026139975
2211	18	-0.15166881734390358
2211	19	-0.3017720366391785
2211	20	-0.345765830871926
2211	21	-0.26499572051746867
2211	22	-0.3946188813577578
2211	23	-0.20831561611325355
2211	24	-0.1753562358174321
2211	25	-0.03267232519824148
2211	26	0.2340367364806184
2211	27	0.06174249148421751
2211	28	-0.14635130921012518
2211	29	-0.3053869451690687
2211	30	-0.0073048283882855
2211	31	-0.21148720453670222
2211	32	-0.06536004160724929
2211	33	-0.08730979831234009
2211	34	0.07374272910479363
2211	35	-0.11199779950544553
2211	36	-0.02216044937466677
2211	37	-0.13097364001585277
2211	38	-0.34593255248206134
2211	39	-0.32569311692710556
2211	40	0.19549103237169874
2211	41	0.2941515805233834
2211	42	0.18165267843721722
2212	1	-1.1876831499982075
2212	2	-0.8326281698935176
2212	3	-1.0432188493485697
2212	4	-0.7975887618066295
2212	5	-0.9698109588378188
2212	6	-1.0948948167313972
2212	7	-1.7830189662590357
2212	8	-1.5392864701474451
2212	9	-0.6220812682639512
2212	10	-0.6661521288553516
2212	11	-1.1146937139402802
2212	12	-1.1210411373988265
2212	13	-0.14197691551128244
2212	14	-1.7510615150625277
2212	15	-0.8471404442065626
2212	16	-0.44284380400205237
2212	17	-0.2880739394000429
2212	18	-0.29518815079458705
2212	19	-0.5861703233257956
2212	20	-0.18013771375639465
2212	21	0.14629337177848356
2212	22	-0.057999741534956684
2212	23	0.13995640172398538
2212	24	-0.3905882959007485
2212	25	-0.36105347875102467
2212	26	-0.31280360852905886
2212	27	-0.2065201383344757
2212	28	-0.01903804276642518
2212	29	-0.22298361242248887
2212	30	-0.30204167049355135
2212	31	-0.6204879673132027
2212	32	-0.44666614968681273
2212	33	-0.26630410682609806
2212	34	-0.1037612314655334
2212	35	-0.2971882002564925
2212	36	-0.4168444838756358
2212	37	-0.44304311135306307
2212	38	-0.5110208585314666
2212	39	-0.3795920783775557
2212	40	-0.4755762679862201
2212	41	-0.03556736567153962
2212	42	-0.31397602542947567
2213	1	-1.2050956891498785
2213	2	-0.8985622358275833
2213	3	-0.9512296790985317
2213	4	-2.0744693691220206
2213	5	-1.7481196067116898
2213	6	-1.1905151384386699
2213	7	-1.954596181314507
2213	8	-2.9629632764329443
2213	9	-0.9633033312251413
2213	10	-0.9416524739209144
2213	11	-0.850246084275259
2213	12	-0.7362664917546127
2213	13	-0.277178911582844
2213	14	-0.14701628493034136
2213	15	-0.2641919916928934
2213	16	-0.28514353325830655
2213	17	-0.1749561393257224
2213	18	-0.01683172461207505
2213	19	-0.2693576673827067
2213	20	-0.2750891391426009
2213	21	-0.4651628048950798
2213	22	-0.4768243951422235
2213	23	-0.6718076796053154
2213	24	-0.3736853920413239
2213	25	-0.20944759671035484
2213	26	0.17572428869448942
2213	27	-0.12789827710391677
2213	28	-0.5177798806386988
2213	29	-0.6375506878591324
2213	30	-0.4258511943030783
2213	31	-0.7576860349460546
2213	32	-0.6200801752747509
2213	33	-0.35932316506254836
2213	34	-0.35532995259946887
2213	35	-0.13538961237094327
2213	36	-0.5451345512961367
2213	37	-0.3022351270668153
2213	38	-0.24635026342608768
2213	39	-0.043487603142644105
2213	40	-0.14770027924065943
2213	41	0.01261440424936211
2213	42	-0.0114968620807403
2214	1	-0.9954606674129423
2214	2	-0.35761592099591866
2214	3	-0.5474686842547314
2214	4	-1.044057883933469
2214	5	-0.6880650116252411
2214	6	-0.6677065245472562
2214	7	-0.8010727761987511
2214	8	-1.65112423083594
2214	9	-0.8279479576532083
2214	10	-0.8655428793399835
2214	11	-0.7638746354834289
2214	12	-0.8228603649726907
2214	13	-0.31889360170517733
2214	14	0.005728634693557666
2214	15	-0.1597536133691655
2214	16	-0.2628808702313066
2214	17	-0.2656850848317376
2214	18	-0.448405882937827
2214	19	-0.011739630783779376
2214	20	-0.15419135327666567
2214	21	-0.2858583867371607
2214	22	-0.5707279438228383
2214	23	-0.6760438539051847
2214	24	-0.38902256725111073
2214	25	-0.3639719117442226
2214	26	-0.49268133257737645
2214	27	-0.25220320077189484
2214	28	0.016281387180526963
2214	29	-0.5336582603998252
2214	30	-0.45809262590756106
2214	31	-0.4051736499495959
2214	32	-0.528350319850631
2214	33	-0.5120276438816802
2214	34	-0.5493548236944008
2214	35	-0.3255523866101555
2214	36	-0.41827174672632594
2214	37	-0.7831470672144221
2214	38	-0.7111465428720507
2214	39	-0.9599874514896314
2214	40	-0.5806292045262751
2214	41	-0.790014248060821
2214	42	-0.3394111275746283
2215	1	0.14361462831044491
2215	2	0.18179490702956258
2215	3	0.10263936852052069
2215	4	-0.08349714689980203
2215	5	0.16259467900260205
2215	6	-0.24081275748628883
2215	7	-0.022552530520862035
2215	8	-0.10363787960754678
2215	9	-0.44703348995530234
2215	10	-0.7909580294764709
2215	11	-0.21641671919589456
2215	12	-0.4386474441355688
2215	13	-0.07162335602728831
2215	14	-0.47499247540653045
2215	15	-0.16944992820083016
2215	16	-0.2577625808773476
2215	17	0.11750417813459535
2215	18	-0.5516531531835085
2215	19	-0.45606401658905327
2215	20	-0.25931532961879356
2215	21	-1.3872858207680991
2215	22	-0.2857529665707901
2215	23	-0.645220378018017
2215	24	-0.6917409475968768
2215	25	-0.8763126760754361
2215	26	-0.7083034890832849
2215	27	-0.7211522453578851
2215	28	-0.6996251187339411
2215	29	-0.5499514815099218
2215	30	-0.9136488133506973
2215	31	-0.549253495263514
2215	32	-0.6598619213065007
2215	33	-0.6645811015704837
2215	34	-0.6145561430756601
2215	35	-0.6296356441169806
2215	36	-0.42023375764534165
2215	37	-0.5592787778604631
2215	38	-0.5681756602514909
2215	39	-0.3830709364759812
2215	40	-0.4714105967009772
2215	41	-0.502167341782382
2215	42	-0.6714174970013786
2216	1	1.3939679171079717
2216	2	1.1507697271734472
2216	3	0.742638083833981
2216	4	0.8007847133263013
2216	5	0.6609245865051676
2216	6	0.8490189485774309
2216	7	1.11963015190463
2216	8	1.0692166938765197
2216	9	0.8444618851731676
2216	10	1.0807981370189061
2216	11	0.9427880598380227
2216	12	0.8498931519589874
2216	13	0.30324817531906234
2216	14	0.12848902510734206
2216	15	0.31625793408077385
2216	16	-0.4914470618639868
2216	17	0.14846512366388254
2216	18	0.6053676587383876
2216	19	0.4141184088991281
2216	20	0.58314998384472
2216	21	0.4418829870427956
2216	22	-0.0846352892840514
2216	23	0.6151855829275164
2216	24	0.2695257533280966
2216	25	0.6133455973058588
2216	26	0.09825483568546645
2216	27	-0.15370620219241715
2216	28	0.13230576513039694
2216	29	0.4452630611416666
2216	30	0.24472091943450636
2216	31	0.4886768747262096
2216	32	0.34652939421251716
2216	33	0.4531925366617884
2216	34	0.2735155424330742
2216	35	0.4965848080926847
2216	36	0.01562826702063802
2216	37	0.20771120158455147
2216	38	0.16730738188623206
2216	39	0.564802907306138
2216	40	0.23802542590950537
2216	41	0.591968064178559
2216	42	0.44279113609214704
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	1.0
25	16.0
26	109.0
27	517.0
28	1285.0
29	2320.0
30	3529.0
31	5118.0
32	7286.0
33	10035.0
34	14998.0
35	23462.0
36	33968.0
37	41697.0
38	21842.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.8109500650069825	92.77158472576684	0.9781143159820869	3.439350893244089
2	3.0533524293227745	1.3130498621959585	3.1014935791741385	92.53210412930713
3	1.9141894331447826	2.144060657118787	94.03598507642316	1.9057648333132746
4	93.11301396653087	4.058176829158217	1.2642689083720957	1.5645402959388144
5	0.9603986015248435	1.8852937459757735	0.9158688418050198	96.23843881069436
6	2.2270629366421355	4.67556849978638	88.45790483984524	4.639463723726254
7	7.33833989240712	84.73841932339242	4.819414858408251	3.103825925792204
8	89.93507079630042	4.965068208760328	2.1115530656332555	2.9883079293059978
9	27.757734908120675	22.977962212331285	22.078743382339656	27.185559497208384
10	28.541593942968575	25.249467361604296	22.541136535984688	23.667802159442445
11	27.099158530859885	21.400883602788042	23.44135598117228	28.05860188517979
12	27.142220831126306	21.631675254008766	25.943203158857802	25.282900756007127
13	40.754249121286534	22.17006114882758	16.965790360633637	20.10989936925225
14	25.746643639011413	27.021790019076047	25.614856448244943	21.616709893667597
15	33.26353034624688	24.261953761538553	22.44286384807019	20.031652044144373
16	35.21780206158272	22.765866544712758	22.103343904009435	19.912987489695094
17	17.95380967854521	23.944229820317485	20.27235199961488	37.82960850152243
18	20.4035699025679	23.506472404267996	19.592337828810773	36.49761986435333
19	23.95595137802383	24.35912865567457	25.11252858346371	26.572391382837885
20	22.59677584349783	26.58394661307113	25.967758434978304	24.85151910845273
21	23.557764632964865	24.510930720952203	24.704693019141548	27.226611626941384
22	26.16693644957667	25.427390286611747	25.05129886931876	23.35437439449282
23	23.838780139966183	27.692964984384684	24.302726512338808	24.165528363310326
24	23.09201302178923	25.158409704963802	27.61233098451707	24.137246288729894
25	22.631233714261644	24.789225686483363	27.48039693572361	25.099143663531382
26	23.280961349837227	26.301125867266805	27.07136108988284	23.346551693013122
27	24.095124049993082	26.733781433720655	26.307143329943493	22.863951186342767
28	25.538111599862802	27.324094522303728	26.2319250464849	20.905868831348574
29	27.10626237340763	25.33833183899677	26.573115180253094	20.982290607342506
30	23.458476498799516	27.935468730255202	25.890734912716706	22.71531985822858
31	24.358982073762945	27.112004380765555	25.446952419349987	23.082061126121516
32	24.91530421282562	25.0910141229849	27.81872995432746	22.17495170986202
33	24.65956204906639	27.43180710421644	26.91911928416264	20.989511562554533
34	24.845651153554538	25.559326521524596	27.04745399622101	22.54756832869986
35	23.615532274661067	27.040672030231732	27.21216971651733	22.131625978589867
36	23.803277110173724	28.008881774910794	26.449757195380997	21.73808391953449
37	23.759951378901572	28.53360452031796	26.182581852536057	21.523862248244406
38	22.841084828171354	28.27906584909407	27.06654711974149	21.813302202993086
39	24.471951762567848	26.655152908327217	27.667320583952147	21.205574745152788
40	22.639306775785293	25.67456974365146	28.7363100252738	22.949813455289444
41	22.951166829020373	25.74768419078275	27.209472582391985	24.0916763978049
42	23.671779565654315	25.845914996299218	26.784048717964147	23.69825672008232
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.5
7	3.0
8	7.5
9	12.0
10	12.0
11	38.5
12	65.0
13	120.5
14	176.0
15	289.5
16	403.0
17	403.0
18	669.5
19	936.0
20	884.0
21	832.0
22	832.0
23	988.5
24	1145.0
25	1671.0
26	2197.0
27	2224.0
28	2251.0
29	2251.0
30	2496.5
31	2742.0
32	3335.0
33	3928.0
34	3928.0
35	4576.0
36	5224.0
37	6114.0
38	7004.0
39	8317.5
40	9631.0
41	9631.0
42	10410.5
43	11190.0
44	12185.0
45	13180.0
46	13418.5
47	13657.0
48	13657.0
49	14970.5
50	16284.0
51	16515.5
52	16747.0
53	16747.0
54	15715.0
55	14683.0
56	12916.0
57	11149.0
58	10564.0
59	9979.0
60	9979.0
61	8687.0
62	7395.0
63	5700.5
64	4006.0
65	3381.0
66	2756.0
67	2756.0
68	2922.5
69	3089.0
70	2157.5
71	1226.0
72	1226.0
73	1342.5
74	1459.0
75	1419.5
76	1380.0
77	987.5
78	595.0
79	595.0
80	695.0
81	795.0
82	421.0
83	47.0
84	47.0
85	32.0
86	17.0
87	8.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.028282074580432415
2	0.003008731338343874
3	0.0018052388030063242
4	0.0
5	0.0012034925353375495
6	0.0
7	6.017462676687747E-4
8	0.0012034925353375495
9	0.09026194015031622
10	0.017450641762394466
11	0.027078582045094866
12	0.028282074580432415
13	0.01865413429773202
14	0.0036104776060126484
15	6.017462676687747E-4
16	0.0
17	6.017462676687747E-4
18	0.009627940282700396
19	0.0018052388030063242
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.006017462676687748
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0012034925353375495
32	0.0
33	0.0
34	6.017462676687747E-4
35	0.0
36	0.0
37	0.0
38	0.0
39	0.003008731338343874
40	0.0018052388030063242
41	0.54518211850791
42	0.0012034925353375495
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
42	166183.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.47423623354976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.69176391603973	21.238032771101736
2	10.064569634500325	6.134201452615491
3	4.304642300021721	3.935420590553787
4	2.827636593408763	3.446802621206742
5	1.9528858874869184	2.975635293622091
6	1.4256659360622397	2.6067648315411325
7	1.1709416898682938	2.497848757093084
8	0.9458365420689927	2.305891697706745
9	0.8411823944079142	2.3070951902420824
>10	6.257528187508639	33.07438185614654
>50	0.32383547578145055	6.709470884506839
>100	0.1816638034871552	10.422847102290849
>500	0.011847639357857946	2.345606951372884
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGCTGGGAGAGCCGCGCCTTGTTCGGTCCGCTGGCAACGC	776	0.4669551037109692	No Hit
ACTGCTGCCTTTGGTACTCAGGCACTGCAGCAAGAGCTGGGC	750	0.45130970075158106	No Hit
ACTGCCCGCGTGTAGTCCTGCACCTCAGGATTAGAGAGGTCG	677	0.4073822232117605	No Hit
ACAGCTAGCAGTGCGGCGCGTGCGCGGGCGGGGCCCGCGTGC	641	0.38571935757568465	No Hit
ACTGCTAGCCCAGTGCAATTTATTGTTGTTTCTCCAAAATCA	546	0.32855346214715103	No Hit
ACTGCTATGGGACTAGTTTTTTTTTTTTTTTTTTTTTTTTTT	508	0.30568710397573756	No Hit
ACTGCTAGGAGGGGGTTGCCACGAATCTTCAGGTTTTAAAAA	486	0.2924486860870245	No Hit
ACTGCTAGCATGCATGAGAAGATAGCAAGTGTGCCAACTAAG	484	0.291245193551687	No Hit
ACTGCATGCGACTTGCCTTTGCGTAACGAAACGCGCAGCATC	449	0.2701840741832799	No Hit
ACTGCTAGAGCTGGAACTGTGGCACCTGCTGGACAGCGTGCC	392	0.2358845369261597	No Hit
ACTGCTGGGAGAGGCCGCGCCTTGTTCGGTCCGCTGGCAACG	347	0.20880595488106485	No Hit
CCTGCTGGCGGGATGGTGTCGTGCGGCAGCACATTGATGGGC	333	0.200381507133702	No Hit
ACTGCATGCGACGCCTCCGGCCTTTGAGCATTGCCTTTGCGT	331	0.19917801459836448	No Hit
CCTGCTGGCGGGACGATGGCCAGGGCCATCAGGGGCTGGCCG	330	0.1985762683306957	No Hit
ACTGCTAGTTAGTCCCACTTTTTCATTAACGTTGACGCCCCT	328	0.1973727757953581	No Hit
ATTGCTATCATGGTCCGACGATCCATGGACGGTAGTTCAGTG	318	0.19135531311867038	No Hit
ACTGCTAGTTCGTACAGTCCGACGATCAAATACTGCTAGTTT	317	0.19075356685100162	No Hit
ACTGCTAGTAAGGGAGGGTAAGATGAAATTATAAGATGTCTT	302	0.18172737283596999	No Hit
ACTGCATGCGACGGCCTCCGGCCTTTGAGCATTGCCTTTGCG	297	0.17871864149762612	No Hit
ACTGCTAGCAGTGGTTCTTTGCAGAACAGATGCTAACTAACA	293	0.176311656426951	No Hit
ACTACTAGACGGCCGACGATCTTGGACTGCAGAACTCTGAAC	290	0.17450641762394467	No Hit
ACTGGGAGAGTCGAGTAGCTTGCACTGATCGTCGGACTGTAG	284	0.17089594001793204	No Hit
ACTGCTAGCTATGTCGACGATCGATGACTGCAGAACTCTGAA	278	0.16728546241191938	No Hit
ACTGCTAGGCAAGAAGGAGACACAGAGCCCAGACCTCCTTTG	275	0.16548022360891307	No Hit
CCTGCTGGCCGAGCTCGACCGCGGCGGCGAGACGCTCTTCGA	275	0.16548022360891307	No Hit
ACTGCTAGGTAACCGACGATCTTGGACTGCAGAACTCTGAAC	269	0.1618697460029004	No Hit
ACTGCTGCCTTTGGGTACTCAGGCACTGCAGCAAGAGCTGGG	256	0.15404704452320633	No Hit
ACTGCTAGCCCAGGTGCAATTTATTGTTGTTTCTCCAAAATC	251	0.1510383131848625	No Hit
ATCGCTAGCATGCTAATGCCTACATTGTAGGCATTAGCAAAT	243	0.14622434304351226	No Hit
ACTGCTAGTTTCGAGGCCCCAGAGAGTGGGGAGGGTAGAGGT	238	0.14321561170516842	No Hit
ATTGCTAGTCGTGATCCGACTATCATCTTCTTTTTCACTCAC	237	0.14261386543749963	No Hit
ACTGCTAGTTAGGTCCCACTTTTTCATTAACGTTGACGCCCC	234	0.14080862663449328	No Hit
ACTGCTAGGGAGCATAAACTGCTAGTTTTTGATCGTCGGATT	226	0.1359946564931431	No Hit
ACTACTAGGTTCCAGTCCGACGATCTCGGACTGTAGAACTCT	218	0.1311806863517929	No Hit
ACTGCTAGCAGTTCTGTTCTTTGCAGAACAGATGCTAACTAA	212	0.12757020874578026	No Hit
ACTACGAGTCCATGGTAGTCGCCGTGCCTACCATGGTGACCA	205	0.12335798487209883	No Hit
ACTGCTGATGGTGACAGGGCCACTCCCACGACCAACACGGCG	204	0.12275623860443007	No Hit
ATTGCTACAGCTCTTCACTAACAATATTTCCAACCAACAATA	203	0.12215449233676128	No Hit
ACTGCATGCGACGGCCGCCGGCCTTTGAGCATTGCCTTTGCG	186	0.1119248057863921	No Hit
ACTGCTAGCAGTGAGGCTGTCTCTGACTGCTGCCTGTCTGGT	183	0.11011956698338578	No Hit
ACGGCTCGTCAGCCGGTGGCCCACAGGAGAGCACAGGCCCAG	181	0.10891607444804823	No Hit
ACTTCTAGGTACCAGGATCAACAGGAAGAGGAGCCAATTCCT	179	0.10771258191271069	No Hit
ACTGCTAAAGCCCAGTCCGACGGACTGTAGAACTCTGAACCC	169	0.10169511923602294	Illumina DpnII expression Sequencing Primer (95% over 24bp)
ACTACTAGTCGTTGTGAATTTCCAGGGAAACTAACATTGATA	168	0.10109337296835416	No Hit
ACTGCTGATGGTGGACAGGGCCACTCCCACGACCAACACGGC	167	0.1004916267006854	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.016848895494725694	0.0	0.0
13	0.0	0.0	0.039715253666139136	0.0	0.0
14	0.0	0.0	0.09206717895332255	0.0	0.0
15	0.0	0.0	0.11072131325105455	0.0	0.0
16	0.0	0.0	0.1311806863517929	0.0	0.0
17	0.0	0.0	0.13479116395780555	0.0	0.0
18	0.0	0.0	0.15886101466455654	0.0	0.0
19	0.0	0.0	0.2220443727697779	0.0	0.0
20	0.0	0.0	0.3923385665200412	0.0	0.0
21	0.0	0.0	0.4723708201199882	0.0	0.0
22	0.0	0.0	1.0813380430007884	0.0	0.0
23	0.0	0.0	1.370777997749469	0.0	0.0
24	0.0	0.0	1.4147054752892896	0.0	0.0
25	0.0	0.0	1.4983482064952491	0.0	0.0
26	0.0	0.0	1.5573193407267891	0.0	0.0
27	0.0	0.0	1.912951384919035	0.0	0.0
28	0.0	0.0	2.0290884145791086	0.0	0.0
29	0.0	0.0	2.226461190374467	0.0	0.0
30	0.0	0.0	2.4238339661698247	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTTCGG	20	0.0020510496	36.180088	36
ATAAAAC	35	6.665214E-7	36.180084	36
AGGTAAC	45	3.3614924E-9	36.027428	7
TCGCATG	30	9.926225E-6	36.027428	6
GGTACGG	20	0.002094085	36.027428	8
GCGACCG	60	1.8189894E-12	36.027428	8
GCGACAT	25	1.4375994E-4	36.027428	8
GAGCTAT	60	1.8189894E-12	36.027428	8
TAGACTA	20	0.002094085	36.027428	6
TAGACGG	60	1.8189894E-12	36.027428	6
TAGACGC	20	0.002094085	36.027428	6
TAGACAC	30	9.926225E-6	36.027428	6
TAGGTTA	20	0.002094085	36.027428	6
TAGGTCT	100	0.0	36.027428	6
CATGAAT	20	0.002094085	36.027428	9
TAGAAAG	40	4.803769E-8	36.027428	6
TAGAAAC	25	1.4375994E-4	36.027428	6
TAGCGCG	45	3.3614924E-9	36.027428	6
TAGCGCA	60	1.8189894E-12	36.027428	6
GAATCTC	20	0.002094085	36.027428	8
>>END_MODULE