FastQCFastQC Report
Thu 26 May 2016
SRR2099137_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099137_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64302
Sequences flagged as poor quality0
Sequence length42
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGCTGCCTTTGGTACTCAGGCACTGCAGCAAGAGCTGGGC2710.42144878852912815No Hit
ACTGCTAGTTTCGAGGCCCCAGAGAGTGGGGAGGGTAGAGGT2380.3701284563466144No Hit
ACTGCTGGGAGAGCCGCGCCTTGTTCGGTCCGCTGGCAACGC2100.32658393207054215No Hit
ACTGCTAGCAGTGAGGCTGTCTCTGACTGCTGCCTGTCTGGT1820.28303940779446984No Hit
ACTGCCCGCGTGTAGTCCTGCACCTCAGGATTAGAGAGGTCG1820.28303940779446984No Hit
ACTGCTATGGGACTAGTTTTTTTTTTTTTTTTTTTTTTTTTT1700.26437746881901025No Hit
ACTGCTAGGAGGGGGTTGCCACGAATCTTCAGGTTTTAAAAA1620.25193617616870395No Hit
ACTGCTAGCCGAGTAGCCTAGAGCGCCAGGGGCGGAAATTCG1390.21616745979907312No Hit
ACTGCTAGCCCAGTGCAATTTATTGTTGTTTCTCCAAAATCA1390.21616745979907312No Hit
ACTGCTAGTCGAGTGGCCTTGCTGTACATCAGTGGGAGGAGA1370.21305713663649653No Hit
ACTGCTAGACCTGAAGCACCAGGAGGGCGAGAAGGTGAAGGG1330.20683649031134335No Hit
ACTGCTAGCATGCATGAGAAGATAGCAAGTGTGCCAACTAAG1260.1959503592423253No Hit
ACTGCATGCGACTTGCCTTTGCGTAACGAAACGCGCAGCATC1180.1835090665920189No Hit
ACAGCTAGCAGTGCGGCGCGTGCGCGGGCGGGGCCCGCGTGC1170.1819539050107306No Hit
ACTGCTAGCGGAGAGATCACGACCATCCTGGCCAACATGGTA1170.1819539050107306No Hit
ATTGCTATCATGGTCCGACGATCCATGGACGGTAGTTCAGTG1030.16018164287269449No Hit
CCTGCTGGCGGGATGGTGTCGTGCGGCAGCACATTGATGGGC1010.15707131971011787No Hit
ACTGCTGCCTTTGGGTACTCAGGCACTGCAGCAAGAGCTGGG980.15240583496625298No Hit
ACTGCTAGTCGAATGGCCTTGCTGTACATCAGTGGGAGGAGA980.15240583496625298No Hit
ACTGCATGCGACGCCTCCGGCCTTTGAGCATTGCCTTTGCGT970.1508506733849647No Hit
ACTGCTAGGCAAGAAGGAGACACAGAGCCCAGACCTCCTTTG970.1508506733849647No Hit
ACTGCTAGAGCTGGAACTGTGGCACCTGCTGGACAGCGTGCC960.1492955118036764No Hit
ACTGCCCGTTCGGCAGCCCTCGGCCAGAGGGGCGCTGCGCAG950.14774035022238813No Hit
ACTGCTGGGAGAGGCCGCGCCTTGTTCGGTCCGCTGGCAACG950.14774035022238813No Hit
CCTGCTGGCGGGACGATGGCCAGGGCCATCAGGGGCTGGCCG940.1461851886410998No Hit
ACTACTAGTGTAGAAGCTTTCGCAAGAAAAATGGCGAGAGCC900.13996454231594663No Hit
ACTGCTAGTTAGTCCCACTTTTTCATTAACGTTGACGCCCCT890.13840938073465833No Hit
ATTGCTAGTCGTGATCCGACTATCATCTTCTTTTTCACTCAC870.13529905757208174No Hit
ACTGCATGCGACGGCCTCCGGCCTTTGAGCATTGCCTTTGCG860.13374389599079345No Hit
ACTGCTAGGCTGGTAGCCTAAAGGTTCTTGCAGAGCAACATC850.13218873440950513No Hit
CCTGCTGGCCGAGCTCGACCGCGGCGGCGAGACGCTCTTCGA850.13218873440950513No Hit
ACTGCCCGTTCGCAGCCCTCGGCCAGAGGGGCGCTGCGCAGC850.13218873440950513No Hit
ACTGCTAGTAAGGGAGGGTAAGATGAAATTATAAGATGTCTT830.12907841124692856No Hit
ACTGCTAGGGGTGGGACTGTCACCTCCAGGCTGTCGTGCTTC820.12752324966564027No Hit
ACTGCTAGGCTCGAAGCCATCGTCCGCACGTGTCTCCTCAAC810.12596808808435198No Hit
ACTGCTAGGACCGTAGCCTAGAGCGCCAGGGGCGGAAATTCG800.12441292650306367No Hit
ACTACTAGACGGCCGACGATCTTGGACTGCAGAACTCTGAAC800.12441292650306367No Hit
ACTGCTAGTTCGTACAGTCCGACGATCAAATACTGCTAGTTT800.12441292650306367No Hit
ACTGCTAGCTTGGTGGCGCACGCCTTTAATCCCAGCACTTGC760.11819228017791049No Hit
ACTGCTAGCTATGTAGCCTAGAGCGCCAGGGGCGGAAATTCG750.11663711859662218No Hit
ACTGCTAGTTACGGGGCTCCATCTGGAGCTCTGGACTACGTG750.11663711859662218No Hit
ACTGCTAGCTTAGTAGCCTCTGCCCCATCGCTTCAGCAGTAG740.1150819570153339No Hit
ACTGCTAGTGGCGAAGGCCAATCCGTTTGGGGGTGCCTCTCA730.11352679543404559No Hit
ACTGCTAGCTATGTCGACGATCGATGACTGCAGAACTCTGAA730.11352679543404559No Hit
ACTGCTAGCTGAGGGGCTTGGGCTCAGATCTGTTTCAGAGAC720.1119716338527573No Hit
ACTGCTAGCCCAGGTGCAATTTATTGTTGTTTCTCCAAAATC700.10886131069018071No Hit
ACTGCTAGCAGTGGTTCTTTGCAGAACAGATGCTAACTAACA700.10886131069018071No Hit
ACTGCTAATGGTAGAGCCTTTACAGGGCCGAAGCCCCAGCTA690.10730614910889243No Hit
ACTGCTAGCAGTGGAGGCTGTCTCTGACTGCTGCCTGTCTGG690.10730614910889243No Hit
ACTGCTAGCCATGAGAGCAGCGGGCAAGGGCAAGGTGGCACA660.10264066436502753No Hit
ACTACGAGTCCATGGTAGTCGCCGTGCCTACCATGGTGACCA660.10264066436502753No Hit
ACTGCTGCCTTTTGGTACTCAGGCACTGCAGCAAGAGCTGGG660.10264066436502753No Hit
ACTGCTAGAGAGGTGGCTCACAACCATCCGTAATGAGATCTG650.10108550278373923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAAGT356.817809E-736.0171246
TAGCGAG356.817809E-736.0171246
TAGGACC950.036.0171246
TAGTGCG356.817809E-736.0171246
GGACCGT356.817809E-736.0171248
TACGGGG356.817809E-736.01712410
TAGTTCC356.817809E-736.0171246
CTAGGAG700.036.0171245
AGACGTG356.817809E-736.0171247
CTAGCGC700.036.0171245
CTAGCGA700.036.0171245
CTAGATA700.036.0171245
GCCGCCA356.817809E-736.01712416
CTAGACG1150.036.0171245
CTAGAAG700.036.0171245
CGCGTGT356.817809E-736.0171247
TAGGCGT356.817809E-736.0171246
TAGGCGG356.817809E-736.0171246
TAGAATC356.817809E-736.0171246
TAGGTCA700.036.0171246
Produced by FastQC (version 0.11.5)