##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099137_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 64302 Sequences flagged as poor quality 0 Sequence length 42 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.175328916674445 37.0 35.0 37.0 33.0 37.0 2 35.83199589437343 37.0 35.0 37.0 35.0 37.0 3 35.755466392958226 37.0 36.0 37.0 35.0 37.0 4 35.630291437280334 37.0 37.0 37.0 35.0 37.0 5 37.58545612889179 39.0 39.0 39.0 35.0 39.0 6 37.45766850175733 39.0 39.0 39.0 35.0 39.0 7 36.517495567789496 37.0 37.0 39.0 33.0 39.0 8 37.21755155360642 39.0 37.0 39.0 34.0 39.0 9 31.48786973966595 33.0 31.0 34.0 30.0 34.0 10 31.899583216696215 33.0 31.0 34.0 30.0 34.0 11 32.04323349195982 34.0 31.0 34.0 30.0 34.0 12 35.49720070915368 37.0 35.0 37.0 35.0 37.0 13 31.265061739914778 31.0 30.0 33.0 30.0 34.0 14 30.9800783801437 31.0 30.0 34.0 27.0 34.0 15 30.74490684582128 31.0 30.0 34.0 26.0 34.0 16 33.081677086249265 35.0 32.0 37.0 26.0 37.0 17 34.25730148362415 35.0 33.0 37.0 30.0 37.0 18 33.94892849367049 35.0 33.0 37.0 30.0 37.0 19 33.18994743553855 35.0 32.0 37.0 26.0 37.0 20 33.3803769711673 35.0 32.0 37.0 27.0 37.0 21 34.92675188952132 37.0 33.0 39.0 27.0 39.0 22 35.314826910516004 37.0 34.0 39.0 29.0 39.0 23 35.97440204037199 37.0 35.0 39.0 30.0 39.0 24 36.336894653354484 37.0 35.0 39.0 32.0 39.0 25 36.532378464122424 38.0 35.0 39.0 32.0 39.0 26 37.88868153401138 39.0 37.0 40.0 33.0 41.0 27 38.08548723212342 40.0 37.0 40.0 33.0 41.0 28 38.35877577680321 40.0 37.0 40.0 34.0 41.0 29 38.45589561755467 40.0 37.0 40.0 35.0 41.0 30 38.51340549283071 40.0 38.0 40.0 35.0 41.0 31 38.736337905508385 40.0 38.0 40.0 35.0 41.0 32 38.79338123231004 40.0 38.0 40.0 36.0 41.0 33 38.8238779509191 40.0 38.0 40.0 36.0 41.0 34 38.8233958508289 40.0 38.0 40.0 36.0 41.0 35 38.828745606668534 40.0 38.0 40.0 36.0 41.0 36 38.75983639700165 40.0 38.0 40.0 35.0 41.0 37 38.692389039221176 40.0 38.0 40.0 35.0 41.0 38 38.720910702622 40.0 38.0 40.0 35.0 41.0 39 38.746213181549564 40.0 38.0 40.0 35.0 41.0 40 38.72297906752512 40.0 38.0 40.0 35.0 41.0 41 38.726540387546265 40.0 38.0 40.0 35.0 41.0 42 38.72510963889148 40.0 38.0 40.0 35.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.9406498083374046 1101 2 0.5332231730602714 1101 3 0.7526567948506084 1101 4 0.7822409148242784 1101 5 0.6910753376429426 1101 6 0.5807146798814884 1101 7 1.0690231714180598 1101 8 1.026264137585585 1101 9 0.8832705604056486 1101 10 1.1331913695668838 1101 11 1.1106917986306755 1101 12 0.6952524318593021 1101 13 -0.1120202983723857 1101 14 0.6342813602393065 1101 15 0.007911770503742588 1101 16 0.5119309201360878 1101 17 -0.7095254788313525 1101 18 -0.6795684974719833 1101 19 -0.0808413676197759 1101 20 -0.8626637465624896 1101 21 -1.1115353405890573 1101 22 -0.8208261315274896 1101 23 -0.13711820334595615 1101 24 -0.0667665120889751 1101 25 0.07095582392594935 1101 26 -0.15663409291600772 1101 27 -0.32384470038380897 1101 28 -0.06366928092135993 1101 29 -0.19054615473488923 1101 30 -0.5030054719486188 1101 31 -0.4817557712281513 1101 32 -0.23430419494767563 1101 33 -0.49755880612953973 1101 34 -0.3571249768350242 1101 35 -0.020757952114124123 1101 36 -0.021652251598709427 1101 37 -0.040210325503394984 1101 38 -0.31167797827979626 1101 39 0.13211205895861866 1101 40 0.026018157758741722 1101 41 0.012214077975933435 1101 42 0.28352569398377625 1102 1 0.6886905606885065 1102 2 0.24456068612192894 1102 3 0.178336000702231 1102 4 0.6066923254825838 1102 5 0.7443668736931031 1102 6 0.8184367279483666 1102 7 0.29956131143895703 1102 8 0.2837092786514148 1102 9 0.28765927513918754 1102 10 0.5411067300684458 1102 11 0.6078182354122816 1102 12 0.7153673743880375 1102 13 -0.14820107162212537 1102 14 -0.21394225522568533 1102 15 -0.5236974248985575 1102 16 -0.49486113838010937 1102 17 -0.3976132531260177 1102 18 0.04443881705257269 1102 19 0.1101199698932831 1102 20 -0.24262717393970945 1102 21 -0.29034934267892254 1102 22 -0.38208527468318465 1102 23 -0.3620398334400008 1102 24 -0.6258051745759161 1102 25 -1.0949271436811614 1102 26 -1.073692609112456 1102 27 -0.7526587024736742 1102 28 -0.714400733376948 1102 29 0.19281330190042922 1102 30 -0.40073274467589215 1102 31 -0.01885086004737957 1102 32 -0.09258528167703872 1102 33 0.35131789188508833 1102 34 0.05862737426215148 1102 35 0.04977496324637798 1102 36 0.23135192812961236 1102 37 0.24806031190517785 1102 38 0.012904048888437103 1102 39 0.25841822405788406 1102 40 0.31311324657797 1102 41 -0.3029635604775649 1102 42 0.04475871383748853 1103 1 0.6126804596784012 1103 2 0.38647987804111494 1103 3 0.2790935764598075 1103 4 0.46729838608864327 1103 5 0.36961939894562335 1103 6 0.6335882430998794 1103 7 0.8786017154793626 1103 8 0.6147698847120182 1103 9 0.3916996791795917 1103 10 0.5186319825936998 1103 11 0.3467071243011688 1103 12 -0.0012992922786310146 1103 13 0.2151827667617141 1103 14 0.04615875487532506 1103 15 0.6818581306569982 1103 16 0.7253408818219071 1103 17 0.5054170499042812 1103 18 -1.1113692637555062 1103 19 -0.17624366647034861 1103 20 -0.04388980020233646 1103 21 -0.0027230800526609755 1103 22 -0.28940850700641363 1103 23 -0.3524438738440452 1103 24 0.20449785572711932 1103 25 0.10658800783399158 1103 26 0.13110537068552475 1103 27 0.562745337930366 1103 28 -0.016925985902204843 1103 29 -0.17713619304906558 1103 30 0.09699452805138264 1103 31 -0.13501247620899193 1103 32 -0.2741509382426983 1103 33 0.13692395249114497 1103 34 -0.25980696917218893 1103 35 0.26391637738778684 1103 36 0.5871600089376869 1103 37 0.4498279886728511 1103 38 0.2848737458581354 1103 39 0.44705458769425377 1103 40 0.5014970849618052 1103 41 -0.13452921704322307 1103 42 0.4275869966657737 1104 1 0.568236015233957 1104 2 0.34203543359667066 1104 3 0.49020468757091606 1104 4 0.7006317194219776 1104 5 0.8140638433900662 1104 6 0.9002549097665451 1104 7 0.6341572710349155 1104 8 0.814769884712021 1104 9 0.14725523473514812 1104 10 0.4186319825936984 1104 11 0.31337379096783735 1104 12 0.4098118188324804 1104 13 -0.1181505665716216 1104 14 -0.16495235623578708 1104 15 0.1374136862125539 1104 16 0.18089643737746286 1104 17 -0.37236072787349883 1104 18 -0.8780359304221719 1104 19 -0.6651325553592358 1104 20 -1.4883342446467793 1104 21 -0.036056413385992414 1104 22 -0.38940850700641505 1104 23 -0.8857772071773766 1104 24 -0.40661325538399495 1104 25 -0.7267453254993441 1104 26 -0.7355612959811424 1104 27 -0.559476884291854 1104 28 -0.11692598590220626 1104 29 -0.1104695263824027 1104 30 -0.25856102750417165 1104 31 -0.5127902539867719 1104 32 -0.08526204935380832 1104 33 -0.06307604750885076 1104 34 0.05130414193892108 1104 35 0.14169415516556683 1104 36 0.27604889782657693 1104 37 0.6387168775617411 1104 38 -0.01512625414186175 1104 39 -0.3751676345279691 1104 40 0.16816375162847663 1104 41 0.15435967184566834 1104 42 -0.0501907811120077 1105 1 0.14867079784265513 1105 2 0.09831562683339001 1105 3 -0.04844265542425319 1105 4 0.20353027014661507 1105 5 -0.5214434029867405 1105 6 -0.27148422066823485 1105 7 -0.02405528935155843 1105 8 0.029262638335211477 1105 9 0.3499122395660663 1105 10 0.06235178935698471 1105 11 -0.04435567763119508 1105 12 -0.27376306039457177 1105 13 -0.28505877913200806 1105 14 0.07755972105889697 1105 15 -0.6758713379420342 1105 16 -0.6727267510283355 1105 17 -0.35907570371890785 1105 18 -0.28214221061541167 1105 19 -0.8740697534268733 1105 20 -0.8429236166274521 1105 21 -1.542819698410149 1105 22 -1.3964133379242867 1105 23 -1.0171781733609535 1105 24 -0.6426035935482446 1105 25 -0.787614890716732 1105 26 -0.4556096051598857 1105 27 -0.5812160147266354 1105 28 -0.4476023144046195 1105 29 -0.5418704925659767 1105 30 -0.8508315589051421 1105 31 -0.5287322829722783 1105 32 -0.03477895756636684 1105 33 -0.4268441634508804 1105 34 -0.7383093846311297 1105 35 -0.5421222699552075 1105 36 0.025324260145417554 1105 37 -0.35717684224501767 1105 38 -0.3462856744317193 1105 39 -0.329032368827491 1105 40 -0.15526620006234992 1105 41 -0.5821137581060256 1105 42 -0.38014247193326156 1106 1 0.21504452587225842 1106 2 0.38624347142173576 1106 3 0.499660952346332 1106 4 0.12119909530850492 1106 5 -0.5448014048368819 1106 6 -0.521021685978134 1106 7 0.20744332304437307 1106 8 0.37292591308081313 1106 9 0.043945542063752896 1106 10 -0.16174626799731584 1106 11 0.05994589498674685 1106 12 0.3696226935369751 1106 13 -0.32784323796641957 1106 14 0.2352840503835978 1106 15 -0.18859104191983533 1106 16 -0.6921532080126056 1106 17 -0.6655049359113221 1106 18 -0.06266950016212292 1106 19 0.3833308016147541 1106 20 0.03034187828466628 1106 21 0.05708779465183511 1106 22 -0.7967371122073601 1106 23 0.17616132710158183 1106 24 -0.038764555620403485 1106 25 -0.4416389425206191 1106 26 -0.46416649692676515 1106 27 -0.11266837365355542 1106 28 0.21451609447604625 1106 29 0.38551156108805174 1106 30 0.09273920890244369 1106 31 0.48791896587137984 1106 32 0.2901516622301159 1106 33 0.2674204063918566 1106 34 0.4418478771635037 1106 35 0.5584790251419278 1106 36 -0.10834826529399066 1106 37 0.10420151113148535 1106 38 -0.08888511939009192 1106 39 0.2151396940772301 1106 40 -0.3266353027450464 1106 41 0.20211380896150644 1106 42 0.1656484623868124 1107 1 0.5561148031127487 1107 2 0.3531465447077835 1107 3 0.5053562027224316 1107 4 0.5258842446745007 1107 5 0.49184162116785046 1107 6 0.5507599602715985 1107 7 0.2149653518429986 1107 8 0.47941634935848754 1107 9 -0.1870881996082865 1107 10 0.20146026542198214 1107 11 -7.676231735800343E-4 1107 12 0.4956704046910687 1107 13 -0.1242111726322257 1107 14 -0.2417200330034639 1107 15 -0.26864691984805233 1107 16 -0.24738639090536196 1107 17 -0.16023951575228068 1107 18 0.0734792210929811 1107 19 -0.5186679088945922 1107 20 -0.01661707292960557 1107 21 0.2841456068160255 1107 22 -0.10051961811752363 1107 23 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1109 10 0.16699799566560003 1109 11 0.3284064707063976 1109 12 -0.22090713541588514 1109 13 -0.3449479521925376 1109 14 -0.13684778107239026 1109 15 -0.3227170327416964 1109 16 -1.4092996410539094 1109 17 -0.3690927540172879 1109 18 -0.7806503095071378 1109 19 -1.0507534703919177 1109 20 -0.26022966948337967 1109 21 -0.6608930146931797 1109 22 -0.6626111213855026 1109 23 -0.6851236124061373 1109 24 -0.28177665407680763 1109 25 0.257568399990852 1109 26 0.11280471709075357 1109 27 -0.06928080586047969 1109 28 -0.06333121466037284 1109 29 -0.09216887278763153 1109 30 0.34013178295334257 1109 31 0.38132739307204844 1109 32 0.23696017286841453 1109 33 0.37025728582447925 1109 34 0.12123878246179487 1109 35 0.4018248741198178 1109 36 0.2623234076305039 1109 37 0.4158410605682761 1109 38 0.3299717850738233 1109 39 0.3647016465177799 1109 40 0.1139153856153996 1109 41 0.29618973720514674 1109 42 0.17399222542393744 1110 1 0.31778556478350595 1110 2 0.10389729545853754 1110 3 0.4016160989823234 1110 4 0.27991099870125424 1110 5 -0.12377399444776671 1110 6 0.0678224773341114 1110 7 -0.37335023647258936 1110 8 -0.4717166017744674 1110 9 -0.28727929979938693 1110 10 0.2369503009120173 1110 11 -0.007046629452581499 1110 12 0.014916923937583704 1110 13 -0.593225641646697 1110 14 -0.37876617004960167 1110 15 -0.494418145619278 1110 16 0.23855409503511993 1110 17 -0.24323159874436584 1110 18 -0.22818608057232126 1110 19 -0.6456130358397161 1110 20 -0.8489949053074355 1110 21 0.017697340367760717 1110 22 0.07665755905964744 1110 23 -0.5428342642344361 1110 24 -0.2891958379665738 1110 25 -0.19341199216600558 1110 26 0.304378643958799 1110 27 0.2288114039964313 1110 28 -0.22893799791421543 1110 29 -0.3287878447007202 1110 30 -0.043546012489159125 1110 31 -0.7100875512840688 1110 32 -0.5288055928973492 1110 33 -0.6973102817430856 1110 34 -0.5456928550580784 1110 35 -0.4174650039935912 1110 36 -0.23476191298423288 1110 37 -0.4381599993151326 1110 38 -0.37909021810583 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2214 40 -0.3542421253228554 2214 41 -0.5085420728742633 2214 42 -0.5626793026914356 2215 1 -0.5968433498454075 2215 2 -0.1468534552922165 2215 3 -0.1209064235401982 2215 4 0.06412378291403797 2215 5 0.27755590688213516 2215 6 0.07961998913162915 2215 7 -0.3579062210285784 2215 8 -0.15983328989115364 2215 9 0.11233459981451333 2215 10 -0.2083521443904246 2215 11 -0.22154684395279745 2215 12 -0.13780722878656348 2215 13 0.21994467152361707 2215 14 -0.28717457845801064 2215 15 0.20249305129191697 2215 16 0.41422977071079714 2215 17 0.42922657371380524 2215 18 0.5648212124349712 2215 19 0.6285182382915551 2215 20 1.065634009321478 2215 21 0.9306102532806761 2215 22 0.6772581596602478 2215 23 0.9427942213940526 2215 24 0.3886248398541028 2215 25 0.4653181665641526 2215 26 0.9279307675109223 2215 27 0.6309993061843358 2215 28 0.5767248077485903 2215 29 0.3228638069509344 2215 30 0.23508976614662203 2215 31 0.0872097460132295 2215 32 0.24489668080492066 2215 33 0.15597157153876395 2215 34 0.3005104911452676 2215 35 0.31312272659413765 2215 36 -0.24934792757024837 2215 37 0.04982798867285254 2215 38 0.10074676173115193 2215 39 0.06451490515456726 2215 40 -0.13818545472073396 2215 41 -0.025005407519415712 2215 42 0.21647588555465802 2216 1 0.18852587030642098 2216 2 -0.224148141282555 2216 3 0.02402111269168472 2216 4 -0.006614657389619083 2216 5 -0.33666079429109175 2216 6 -0.2388755250160628 2216 7 0.12449543528612139 2216 8 0.05824814558158664 2216 9 -0.20443558652089067 2216 10 -0.013735167164753648 2216 11 0.11868780062967232 2216 12 0.04507751931557635 2216 13 0.29827455420132765 2216 14 0.2804582717835373 2216 15 0.7045634446666611 2216 16 0.5301717996963049 2216 17 0.361938789034717 2216 18 0.3446693835874868 2216 19 0.8686838697615329 2216 20 0.8599749340971812 2216 21 1.174571605937679 2216 22 0.9622823142496202 2216 23 0.714705884610062 2216 24 0.6291355368865368 2216 25 0.6689068484137053 2216 26 0.09149184411548106 2216 27 0.12892891280959873 2216 28 0.32413681603016187 2216 29 -0.027377738942789165 2216 30 0.07670467297892003 2216 31 0.2683691663030814 2216 32 -0.28477895756636684 2216 33 0.10214134379549478 2216 34 0.026183368992057865 2216 35 -0.132701980100137 2216 36 0.11952715869614394 2216 37 -0.06369858137545492 2216 38 0.012409977742194656 2216 39 0.102127051462368 2216 40 -0.04294735948263906 2216 41 -0.03501230883065887 2216 42 -0.5178236313535507 >>END_MODULE >>Per sequence quality scores pass #Quality Count 25 2.0 26 15.0 27 49.0 28 201.0 29 387.0 30 649.0 31 1131.0 32 1962.0 33 3521.0 34 6407.0 35 11212.0 36 15082.0 37 11035.0 38 12649.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 1.7402528732057043 95.48840609010746 0.4167897855398828 2.3545512511469497 2 1.6904606388603778 0.5023171907561196 1.8693042207085317 95.93791794967497 3 0.9377770174647362 0.8957870017573598 97.38262235424021 0.78381362653769 4 95.177443936425 3.2425118969860964 0.5831855929831109 0.9968585736057977 5 0.4976671850699844 0.76049766718507 0.47433903576982894 98.26749611197512 6 1.2223570028926005 2.9890205592361045 92.63164442785606 3.1569780100152407 7 5.455591670425032 89.22722819240758 3.111926719646662 2.205253417520723 8 92.7760497667185 3.701399688958009 1.463452566096423 2.0590979782270606 9 26.998864679077432 22.418700135305368 23.49181169225027 27.090623493366927 10 28.676070356604093 24.441299513226856 23.212702757344363 23.669927372824684 11 27.604012753713352 20.800995411773854 24.43735904813749 27.157632786375302 12 26.403620641388535 22.607235061743754 25.761298951755883 25.22784534511182 13 53.083539421087835 20.92762820213709 10.718118613223835 15.27071376355124 14 24.587104600167958 30.338092127772075 28.936891543031322 16.137911729028644 15 45.26990248985241 24.73522962317849 16.243915335686847 13.75095255128225 16 51.07306149108892 19.000964200180398 16.901496065441197 13.024478243289478 17 14.539431735120761 20.724405530240585 13.97023374442077 50.76592899021788 18 14.321932403216525 22.150156315617565 15.541349758138523 47.98656152302739 19 23.01591004525731 25.12791800805611 26.222802842968008 25.633369103718568 20 21.868682156076016 28.447015644925504 25.759696432459332 23.92460576653914 21 23.730210568878107 23.602687319212468 23.711548629902644 28.955553482006778 22 28.58853534882274 25.22783117165874 24.134552580013064 22.04908089950546 23 24.829709806848932 28.935336381450032 22.70380392522783 23.531149886473205 24 23.254331125003887 24.761282697272247 26.520170445709308 25.464215732014555 25 23.492270846940997 24.341389070324407 26.65702466486268 25.50931541787192 26 24.06145998569251 26.09250101085503 26.796989207178623 23.04904979627383 27 23.723989922552953 27.028708282790582 25.57929768902989 23.668004105626576 28 25.686603838138783 27.28686510528444 25.417560884575906 21.60897017200087 29 29.12351093278592 24.5948804080744 25.546639295822835 20.73496936331685 30 25.456439924108114 27.3723989922553 25.08475630618021 22.086404777456377 31 26.063763608087093 26.22706065318818 24.0 23.709175738724728 32 25.610400920655657 24.22941743647165 27.433050293925536 22.727131348947154 33 25.92609872165718 26.136045535131103 26.02096357811577 21.916892165095952 34 25.417956174865086 25.35263837265361 26.127120884589665 23.102284567891633 35 24.55600136854219 25.969643245933256 27.529470311965415 21.944885073559142 36 25.101863083574383 26.479736244595813 25.585518335355044 22.83288233647476 37 24.870144007962427 28.82803023234114 24.944791763864266 21.357033995832168 38 23.803303163198656 28.258841093589627 25.930764206401047 22.007091536810673 39 25.09292813039489 25.553291755447376 27.117905967619016 22.23587414653872 40 23.88373069565623 24.39851319616168 28.648968102147776 23.068788006034307 41 24.332058100836676 23.682000559858167 27.260427358402538 24.725513980902615 42 24.695178849144632 24.956454121306376 26.618973561430792 23.729393468118197 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.5 7 3.0 8 5.5 9 8.0 10 8.0 11 15.0 12 22.0 13 44.0 14 66.0 15 103.0 16 140.0 17 140.0 18 217.0 19 294.0 20 251.0 21 208.0 22 208.0 23 229.0 24 250.0 25 375.0 26 500.0 27 528.0 28 556.0 29 556.0 30 649.5 31 743.0 32 930.5 33 1118.0 34 1118.0 35 1325.5 36 1533.0 37 1895.0 38 2257.0 39 2685.5 40 3114.0 41 3114.0 42 3379.5 43 3645.0 44 4222.5 45 4800.0 46 5021.5 47 5243.0 48 5243.0 49 5978.0 50 6713.0 51 7042.5 52 7372.0 53 7372.0 54 6977.0 55 6582.0 56 5983.0 57 5384.0 58 5115.0 59 4846.0 60 4846.0 61 4185.5 62 3525.0 63 2578.5 64 1632.0 65 1361.5 66 1091.0 67 1091.0 68 1057.0 69 1023.0 70 674.0 71 325.0 72 325.0 73 391.5 74 458.0 75 491.5 76 525.0 77 350.0 78 175.0 79 175.0 80 160.5 81 146.0 82 74.5 83 3.0 84 3.0 85 2.5 86 2.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0015551615812882957 2 0.0 3 0.0015551615812882957 4 0.0 5 0.0031103231625765914 6 0.0 7 0.0015551615812882957 8 0.0031103231625765914 9 0.004665484743864887 10 0.0015551615812882957 11 0.010886131069018071 12 0.006220646325153183 13 0.013996454231594662 14 0.0 15 0.0015551615812882957 16 0.0 17 0.0015551615812882957 18 0.013996454231594662 19 0.004665484743864887 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0031103231625765914 32 0.0 33 0.0 34 0.0015551615812882957 35 0.0 36 0.0 37 0.0 38 0.0 39 0.007775807906441479 40 0.004665484743864887 41 0.0 42 0.0031103231625765914 >>END_MODULE >>Sequence Length Distribution pass #Length Count 42 64302.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.56187987931946 #Duplication Level Percentage of deduplicated Percentage of total 1 59.749857873791925 21.248172685141988 2 14.40066471334237 10.242294174364716 3 7.849739799711375 8.374545115237474 4 5.094677920146936 7.24705296880346 5 3.485371933353741 6.197318901433859 6 2.260899986880658 4.824111225156294 7 1.6530371277386626 4.11495754408883 8 0.9795775571784668 2.786849553668626 9 0.7259369396947567 2.323411402444714 >10 3.4809988192591943 22.207707380796865 >50 0.2448943892946167 6.320176666355634 >100 0.07434293960729435 4.113402382507543 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACTGCTGCCTTTGGTACTCAGGCACTGCAGCAAGAGCTGGGC 271 0.42144878852912815 No Hit ACTGCTAGTTTCGAGGCCCCAGAGAGTGGGGAGGGTAGAGGT 238 0.3701284563466144 No Hit ACTGCTGGGAGAGCCGCGCCTTGTTCGGTCCGCTGGCAACGC 210 0.32658393207054215 No Hit ACTGCTAGCAGTGAGGCTGTCTCTGACTGCTGCCTGTCTGGT 182 0.28303940779446984 No Hit ACTGCCCGCGTGTAGTCCTGCACCTCAGGATTAGAGAGGTCG 182 0.28303940779446984 No Hit ACTGCTATGGGACTAGTTTTTTTTTTTTTTTTTTTTTTTTTT 170 0.26437746881901025 No Hit ACTGCTAGGAGGGGGTTGCCACGAATCTTCAGGTTTTAAAAA 162 0.25193617616870395 No Hit ACTGCTAGCCGAGTAGCCTAGAGCGCCAGGGGCGGAAATTCG 139 0.21616745979907312 No Hit ACTGCTAGCCCAGTGCAATTTATTGTTGTTTCTCCAAAATCA 139 0.21616745979907312 No Hit ACTGCTAGTCGAGTGGCCTTGCTGTACATCAGTGGGAGGAGA 137 0.21305713663649653 No Hit ACTGCTAGACCTGAAGCACCAGGAGGGCGAGAAGGTGAAGGG 133 0.20683649031134335 No Hit ACTGCTAGCATGCATGAGAAGATAGCAAGTGTGCCAACTAAG 126 0.1959503592423253 No Hit ACTGCATGCGACTTGCCTTTGCGTAACGAAACGCGCAGCATC 118 0.1835090665920189 No Hit ACAGCTAGCAGTGCGGCGCGTGCGCGGGCGGGGCCCGCGTGC 117 0.1819539050107306 No Hit ACTGCTAGCGGAGAGATCACGACCATCCTGGCCAACATGGTA 117 0.1819539050107306 No Hit ATTGCTATCATGGTCCGACGATCCATGGACGGTAGTTCAGTG 103 0.16018164287269449 No Hit CCTGCTGGCGGGATGGTGTCGTGCGGCAGCACATTGATGGGC 101 0.15707131971011787 No Hit ACTGCTGCCTTTGGGTACTCAGGCACTGCAGCAAGAGCTGGG 98 0.15240583496625298 No Hit ACTGCTAGTCGAATGGCCTTGCTGTACATCAGTGGGAGGAGA 98 0.15240583496625298 No Hit ACTGCATGCGACGCCTCCGGCCTTTGAGCATTGCCTTTGCGT 97 0.1508506733849647 No Hit ACTGCTAGGCAAGAAGGAGACACAGAGCCCAGACCTCCTTTG 97 0.1508506733849647 No Hit ACTGCTAGAGCTGGAACTGTGGCACCTGCTGGACAGCGTGCC 96 0.1492955118036764 No Hit ACTGCCCGTTCGGCAGCCCTCGGCCAGAGGGGCGCTGCGCAG 95 0.14774035022238813 No Hit ACTGCTGGGAGAGGCCGCGCCTTGTTCGGTCCGCTGGCAACG 95 0.14774035022238813 No Hit CCTGCTGGCGGGACGATGGCCAGGGCCATCAGGGGCTGGCCG 94 0.1461851886410998 No Hit ACTACTAGTGTAGAAGCTTTCGCAAGAAAAATGGCGAGAGCC 90 0.13996454231594663 No Hit ACTGCTAGTTAGTCCCACTTTTTCATTAACGTTGACGCCCCT 89 0.13840938073465833 No Hit ATTGCTAGTCGTGATCCGACTATCATCTTCTTTTTCACTCAC 87 0.13529905757208174 No Hit ACTGCATGCGACGGCCTCCGGCCTTTGAGCATTGCCTTTGCG 86 0.13374389599079345 No Hit ACTGCTAGGCTGGTAGCCTAAAGGTTCTTGCAGAGCAACATC 85 0.13218873440950513 No Hit CCTGCTGGCCGAGCTCGACCGCGGCGGCGAGACGCTCTTCGA 85 0.13218873440950513 No Hit ACTGCCCGTTCGCAGCCCTCGGCCAGAGGGGCGCTGCGCAGC 85 0.13218873440950513 No Hit ACTGCTAGTAAGGGAGGGTAAGATGAAATTATAAGATGTCTT 83 0.12907841124692856 No Hit ACTGCTAGGGGTGGGACTGTCACCTCCAGGCTGTCGTGCTTC 82 0.12752324966564027 No Hit ACTGCTAGGCTCGAAGCCATCGTCCGCACGTGTCTCCTCAAC 81 0.12596808808435198 No Hit ACTGCTAGGACCGTAGCCTAGAGCGCCAGGGGCGGAAATTCG 80 0.12441292650306367 No Hit ACTACTAGACGGCCGACGATCTTGGACTGCAGAACTCTGAAC 80 0.12441292650306367 No Hit ACTGCTAGTTCGTACAGTCCGACGATCAAATACTGCTAGTTT 80 0.12441292650306367 No Hit ACTGCTAGCTTGGTGGCGCACGCCTTTAATCCCAGCACTTGC 76 0.11819228017791049 No Hit ACTGCTAGCTATGTAGCCTAGAGCGCCAGGGGCGGAAATTCG 75 0.11663711859662218 No Hit ACTGCTAGTTACGGGGCTCCATCTGGAGCTCTGGACTACGTG 75 0.11663711859662218 No Hit ACTGCTAGCTTAGTAGCCTCTGCCCCATCGCTTCAGCAGTAG 74 0.1150819570153339 No Hit ACTGCTAGTGGCGAAGGCCAATCCGTTTGGGGGTGCCTCTCA 73 0.11352679543404559 No Hit ACTGCTAGCTATGTCGACGATCGATGACTGCAGAACTCTGAA 73 0.11352679543404559 No Hit ACTGCTAGCTGAGGGGCTTGGGCTCAGATCTGTTTCAGAGAC 72 0.1119716338527573 No Hit ACTGCTAGCCCAGGTGCAATTTATTGTTGTTTCTCCAAAATC 70 0.10886131069018071 No Hit ACTGCTAGCAGTGGTTCTTTGCAGAACAGATGCTAACTAACA 70 0.10886131069018071 No Hit ACTGCTAATGGTAGAGCCTTTACAGGGCCGAAGCCCCAGCTA 69 0.10730614910889243 No Hit ACTGCTAGCAGTGGAGGCTGTCTCTGACTGCTGCCTGTCTGG 69 0.10730614910889243 No Hit ACTGCTAGCCATGAGAGCAGCGGGCAAGGGCAAGGTGGCACA 66 0.10264066436502753 No Hit ACTACGAGTCCATGGTAGTCGCCGTGCCTACCATGGTGACCA 66 0.10264066436502753 No Hit ACTGCTGCCTTTTGGTACTCAGGCACTGCAGCAAGAGCTGGG 66 0.10264066436502753 No Hit ACTGCTAGAGAGGTGGCTCACAACCATCCGTAATGAGATCTG 65 0.10108550278373923 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.006220646325153184 0.0 0.0 13 0.0 0.0 0.017106777394171255 0.0 0.0 14 0.0 0.0 0.054430655345090354 0.0 0.0 15 0.0 0.0 0.05598581692637865 0.0 0.0 16 0.0 0.0 0.05598581692637865 0.0 0.0 17 0.0 0.0 0.05598581692637865 0.0 0.0 18 0.0 0.0 0.059096140088955244 0.0 0.0 19 0.0 0.0 0.10264066436502753 0.0 0.0 20 0.0 0.0 0.20839165189263165 0.0 0.0 21 0.0 0.0 0.25038101458741563 0.0 0.0 22 0.0 0.0 0.8677801623588691 0.0 0.0 23 0.0 0.0 1.088613106901807 0.0 0.0 24 0.0 0.0 1.1134956922024197 0.0 0.0 25 0.0 0.0 1.1585953780597804 0.0 0.0 26 0.0 0.0 1.1865882865229698 0.0 0.0 27 0.0 0.0 1.340549283070511 0.0 0.0 28 0.0 0.0 1.3949799384156014 0.0 0.0 29 0.0 0.0 1.561382227613449 0.0 0.0 30 0.0 0.0 1.6904606388603776 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGAAGT 35 6.817809E-7 36.017124 6 TAGCGAG 35 6.817809E-7 36.017124 6 TAGGACC 95 0.0 36.017124 6 TAGTGCG 35 6.817809E-7 36.017124 6 GGACCGT 35 6.817809E-7 36.017124 8 TACGGGG 35 6.817809E-7 36.017124 10 TAGTTCC 35 6.817809E-7 36.017124 6 CTAGGAG 70 0.0 36.017124 5 AGACGTG 35 6.817809E-7 36.017124 7 CTAGCGC 70 0.0 36.017124 5 CTAGCGA 70 0.0 36.017124 5 CTAGATA 70 0.0 36.017124 5 GCCGCCA 35 6.817809E-7 36.017124 16 CTAGACG 115 0.0 36.017124 5 CTAGAAG 70 0.0 36.017124 5 CGCGTGT 35 6.817809E-7 36.017124 7 TAGGCGT 35 6.817809E-7 36.017124 6 TAGGCGG 35 6.817809E-7 36.017124 6 TAGAATC 35 6.817809E-7 36.017124 6 TAGGTCA 70 0.0 36.017124 6 >>END_MODULE