SRR2099136_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2099136_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	290103
Sequences flagged as poor quality	0
Sequence length	47
%GC	47

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ACTGCTACCATTTGCATCTTTGAACTATACCAGTTCTCAGATCCCTA	3894	1.342281879194631	No Hit
ACTGCTAGCAATTAGGACGACTCACTATAGTGAGTCGTATTACCGGC	1757	0.6056469598728728	No Hit
ACCGCCAGCACGTGAATATCGCCTGCCGCGCCCTCGCCGTGCCGACC	1318	0.4543213961937656	No Hit
ACTGCTATGAAAACGCCACGATCGTCGGACTGTAGAACTCTGAACCC	1155	0.39813445569332273	Illumina DpnII expression Sequencing Primer (96% over 27bp)
ACTGCTACGACCAAGCGAAAACCGTAACCGGCACTGCCCGCCACCAC	1110	0.3826227236533231	No Hit
ACTGCTAGGTCGTCGATATCGTACCGCAATCAGGTCAACTGCTAGTT	1007	0.3471180925395463	No Hit
ACTGCTATGAAAACGCCCTACAGTCCTACGATCGTCGGACTGTAGAA	878	0.30265112735821414	No Hit
ACTGCTAGTTAGGGACGTCTCAAAAACAAAAACAAAAACAGCAGGGG	847	0.29196526750843665	No Hit
ACTGCTAGCATCAGTAGGGCGGAAGTGCCCAGCGTCAGTGCGCTGGC	766	0.26404414983643737	No Hit
ACTGCTAGCGATGTGGACAGTCCGACGATCGTACAACTTCTGTGTCA	665	0.22922892903554942	No Hit
ACTGCTAGCGTGTTACTGGGACGGCCTGATGCTGTCATGCTCTCCAC	654	0.22543717231466065	No Hit
ACTGCTCTGAATTCGAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG	543	0.18717489994932834	No Hit
ACTGCTATGAAAACGCCCTACAGTCCGACGATCGTCGGACTGTAGAA	506	0.17442080916088423	No Hit
ACTGCTAGTCTAGCGAACAGTCCGATAATCGTACGTGTGAGCCGTCA	501	0.17269728337866208	No Hit
ACTGCTATGAAAACGCCATAGCAGTTGACGGCGTTTTCGTACGATCG	487	0.16787141118843998	No Hit
ACTGCTATGAAAACGCCAGTGAGTCGTATTACCGGCTCACTATAGCA	476	0.16407965446755118	No Hit
ACTGCTAGAAGCTGCTACCGGCCGACGATCGTACTAGCAGTTGACTA	464	0.15994319259021794	No Hit
ACTGCTAGAGACCGGGGGTCAACATCCAGCTGTGGCTCTGCACTCAG	457	0.1575302564951069	No Hit
ACTGCATGAGTCGTCAGTCCGACGATCGTACCCGCGGGAAGTCAACT	432	0.14891262758399604	No Hit
ACTGCTAGTTAGGGACGGTGGTGACATGTGCACCTGTAGTCCCAGCT	429	0.14787851211466274	No Hit
ACTGCTAGAAGCTGCTACGGCCGACGATCGTACTAGCAGTTGACTAG	428	0.1475338069582183	No Hit
ACTGCTATGAAAACGCCTAGTGAGTCGTATTACCGGCTCACTATAGC	409	0.14098440898577402	No Hit
ACTGTTAGGAGATTTAAACTGCTAGTTTTTTTTTTTTTTTTTTTTTT	395	0.13615853679555193	No Hit
ACTGCTAGAACAGGTTACAGTCCGACGATCTTACCAAACACAAGTCA	387	0.13340089554399642	No Hit
ACTGCTAGGCTGGTGGACAGACCTACCTGCCATCATCACATGGCTGC	375	0.1292644336666632	No Hit
ACTGCTAGAGGATGAACCCGACGATCGTACAGTCGTTTCGTCGGACT	372	0.1282303181973299	No Hit
ACTGCTATGAAAACGCCGTTGACGGCGTTTTCGTACGATCGTCGGAC	357	0.12305974085066339	No Hit
ACTGCTAGGTGATGAGTATGAATACAACAAAGAGTCTAACTGAAAAT	350	0.12064680475555234	No Hit
ACTGCTACCATTTGCATCGGCCGACGATCGTAGCAGTTGACATGCAA	343	0.11823386866044129	No Hit
ACTGCTAGAACGACCTTGACGATCGTACCGAGCCATTGTCAACTGTA	340	0.11719975319110798	No Hit
ACTGCTATGAAAACGCCTATAGTGAGTCGTATTACCGGCTCACTATA	322	0.11099506037510815	No Hit
ACTGCGAGCCGACTCTAGTCCGACGATCGTACGACGGCGGACTGTAG	311	0.10720330365421936	No Hit
ACTGCTAGGTTATAAACACCATAAACCCCACCCACACCTGTTAGAGA	301	0.10375625208977501	No Hit
ACTGCTTACGGACACCGAGACAAGCCCAAATATGCAGAAATTGCCGA	300	0.10341154693333057	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGCGT	25	6.726029E-5	41.000343	18
CTAGATA	335	0.0	40.99328	5
TTGATAG	25	6.732872E-5	40.99328	26
AGCAACC	65	0.0	40.99328	7
AGCCGGA	35	2.51257E-7	40.99328	7
TTGGCGC	30	4.1019484E-6	40.99328	5
CGTGCGT	55	3.6379788E-12	40.99328	9
TAGACGG	185	0.0	40.99328	6
GGCACCG	30	4.1019484E-6	40.99328	34
TTTAACG	30	4.1019484E-6	40.99328	9
AGCTTAT	25	6.732872E-5	40.99328	7
TAGCGGA	110	0.0	40.99328	6
TAGCGCT	55	3.6379788E-12	40.99328	6
GTCTACC	95	0.0	40.99328	9
GCGCATA	60	0.0	40.99328	9
GCGTTCG	45	9.549694E-10	40.99328	8
AGAGTAT	30	4.1019484E-6	40.99328	7
GCTTACG	45	9.549694E-10	40.99328	4
GTCGTGC	25	6.732872E-5	40.99328	8
TTGCGTG	60	0.0	40.99328	9

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content