FastQCFastQC Report
Thu 26 May 2016
SRR2099135_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099135_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159600
Sequences flagged as poor quality0
Sequence length47
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGCTACCATTTGCATCTTTGAACTATACCAGTTCTCAGATCCCTA24761.5513784461152882No Hit
ACTGCTAGCAATTAGGACGACTCACTATAGTGAGTCGTATTACCGGC13830.8665413533834586No Hit
ACCGCCAGCACGTGAATATCGCCTGCCGCGCCCTCGCCGTGCCGACC8540.5350877192982456No Hit
ACTGCTACGACCAAGCGAAAACCGTAACCGGCACTGCCCGCCACCAC7650.4793233082706767No Hit
ACTGCTAGGTCGTCGATATCGTACCGCAATCAGGTCAACTGCTAGTT5540.34711779448621555No Hit
ACTGCTAGTTAGGGACGTCTCAAAAACAAAAACAAAAACAGCAGGGG5500.34461152882205515No Hit
ACTGCTAGCATCAGTAGGGCGGAAGTGCCCAGCGTCAGTGCGCTGGC4660.2919799498746867No Hit
ACTGCTATGAAAACGCCACGATCGTCGGACTGTAGAACTCTGAACCC4290.268796992481203Illumina DpnII expression Sequencing Primer (96% over 27bp)
ACTGCTAGCGTGTTACTGGGACGGCCTGATGCTGTCATGCTCTCCAC4150.2600250626566416No Hit
ACTGCTAGCGATGTGGACAGTCCGACGATCGTACAACTTCTGTGTCA4000.2506265664160401No Hit
ACTGCTCTGAATTCGAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG3580.2243107769423559No Hit
ACTGCTATGAAAACGCCCTACAGTCCGACGATCGTCGGACTGTAGAA3290.20614035087719298No Hit
ACTGCTATGAAAACGCCATAGCAGTTGACGGCGTTTTCGTACGATCG3150.19736842105263158No Hit
ACTGCTATGAAAACGCCAGTGAGTCGTATTACCGGCTCACTATAGCA3150.19736842105263158No Hit
ACTGCTATGAAAACGCCCTACAGTCCTACGATCGTCGGACTGTAGAA3110.19486215538847118No Hit
ACTGCTAGTCTAGCGAACAGTCCGATAATCGTACGTGTGAGCCGTCA3100.19423558897243107No Hit
ACTGCTAGAAGCTGCTACCGGCCGACGATCGTACTAGCAGTTGACTA2980.1867167919799499No Hit
ACTGCTAGAGACCGGGGGTCAACATCCAGCTGTGGCTCTGCACTCAG2950.18483709273182958No Hit
ACTGCATGAGTCGTCAGTCCGACGATCGTACCCGCGGGAAGTCAACT2800.17543859649122806No Hit
ACTGCTAGTTAGGGACGGTGGTGACATGTGCACCTGTAGTCCCAGCT2760.17293233082706766No Hit
ACTGTTAGGAGATTTAAACTGCTAGTTTTTTTTTTTTTTTTTTTTTT2730.17105263157894737No Hit
ACTGCTAGAAGCTGCTACGGCCGACGATCGTACTAGCAGTTGACTAG2690.16854636591478697No Hit
ACTGCTAGAACAGGTTACAGTCCGACGATCTTACCAAACACAAGTCA2610.16353383458646617No Hit
ACTGCTATGAAAACGCCTAGTGAGTCGTATTACCGGCTCACTATAGC2580.16165413533834586No Hit
ACTGCTAGGCTGGTGGACAGACCTACCTGCCATCATCACATGGCTGC2530.15852130325814537No Hit
ACTGCTAGAGGATGAACCCGACGATCGTACAGTCGTTTCGTCGGACT2420.15162907268170425No Hit
ACTGCTAGGTGATGAGTATGAATACAACAAAGAGTCTAACTGAAAAT2320.14536340852130328No Hit
ACTGCTACCATTTGCATCGGCCGACGATCGTAGCAGTTGACATGCAA2260.14160401002506265No Hit
ACTGCGAGCCGACTCTAGTCCGACGATCGTACGACGGCGGACTGTAG2110.13220551378446116No Hit
ACTGCTATTGGTGCTCTGAGTTCTACAGTCCGACGATCGTCGGACTG2100.13157894736842105Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACTGCTATGAAAACGCCTATAGTGAGTCGTATTACCGGCTCACTATA2090.13095238095238096No Hit
ACTGCTAGAACGACCTTGACGATCGTACCGAGCCATTGTCAACTGTA2030.12719298245614036No Hit
ACTGCTATGAAAACGCCGTTGACGGCGTTTTCGTACGATCGTCGGAC1950.12218045112781956No Hit
ACTGCTAGGTTATAAACACCATAAACCCCACCCACACCTGTTAGAGA1930.12092731829573934No Hit
ACTGCTTACGGACACCGAGACAAGCCCAAATATGCAGAAATTGCCGA1920.12030075187969924No Hit
ACTGCTAGGATAATGATTGGCGATGTAGGTGGCGATGTAGACGCCGG1880.11779448621553885No Hit
ACTGCTAGAGGGCAACCCGTACACTGGCATAGTCAACTGCTAGTTTA1830.11466165413533834No Hit
ACTGCTAGAGAACAACCCAGTCCGACGATCGTACTGCATTGTGGTCA1820.11403508771929824No Hit
ACTGCTAGGACTTTAAGAGTCCGACGATCGTACAATACTCGAGTCAA1770.11090225563909774No Hit
ACTGCTACCATTTGCATCCGGCCGACGATCGTAGCAGTTGACATGCA1760.11027568922305764No Hit
ACTGCTATGAAAACGCCACTCTGAACCCCTATAGTGAGTCGTATTAC1750.10964912280701754No Hit
ACTGCTAGGTTGATAAGCAATTTAGCCCCAAGATATTGGGCCATTGT1740.10902255639097744No Hit
ACTGCTACCATTTGCATCCTTTGAACTATACCAGTTCTCAGATCCCT1740.10902255639097744No Hit
CCTGCTAGGAGCAGGTTGAGTTCTACAGTCCGACGATCGTACCTGTT1720.10776942355889725Illumina Small RNA Adapter 1 (95% over 22bp)
ACTGCCAAAAAATCGAGTTTTGACTGTGTGGGAGTCAGTGCCCCTAA1710.10714285714285715No Hit
ACTGCTAGTAAAAGATAGTCCGACGATCGTACAAATGAGAGGTCAAC1690.10588972431077694No Hit
ACTGCTATGAAAACGCCATAGTGAGTCGTATTACCGGCTCACTATAG1660.10401002506265664No Hit
ACTGCTAGTTGAGCATCGTCCGACGATCGTACGTAGGATGAGTCAAC1650.10338345864661654No Hit
ACTGCTAGTGTAACCGGCAGAAATACAGGGAGCGATTTCATAAAGTA1630.10213032581453635No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAACC200.001106456341.0213221
GGTTCCC200.001106456341.0213223
ACTGGCC200.001106456341.0213223
TCCGATA459.422365E-1041.02131721
CCGATAA459.422365E-1041.02131722
CGATAAT459.422365E-1041.02131723
GGTCCAC200.001108164541.00846517
CCCCGAC200.001108164541.00846517
CGAGTTC304.0799223E-641.0084617
GTCTCAA304.0799223E-641.0084617
ATCAACT352.4958172E-741.0084617
GCAATTT304.0799223E-641.0084617
ATACCGA256.704568E-541.0084617
TTCTAGT200.001109874940.9956173
TACCCGC200.001109874940.99561729
ACGCCTC401.5379555E-840.99561713
CGTATGG200.001109874940.9956179
GATAACA200.001109874940.9956178
TGCTTGC401.5379555E-840.9956173
ATCTAAA401.5379555E-840.9956177
Produced by FastQC (version 0.11.5)