FastQCFastQC Report
Thu 26 May 2016
SRR2099134_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099134_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387931
Sequences flagged as poor quality0
Sequence length47
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTGACTCGGAAGAATGTCCGACGATCCAGTTTCCAATGATCGAAC11640.3000533600047431No Hit
ACGTGACTCAAACAGCTTTCTACAGTCCGACGCTCCAGTAATTGGTG10260.2644800235093354No Hit
ACGTGAGCAGCTATAGCCATTGGTGTCCTTTATCCAGATCAGTATGG9840.2536533558802983No Hit
ACGTGACTTCGGGCTCATCGAACGTGACTTTTTTTTTTTTTTTTTTT9730.25081780007269333No Hit
ACGTGACTACGAACACCTCCGACGATCCAGTTTGATAGCGTCGAACT8440.2175644637835079No Hit
ACGTGACTAAATCGGGACAGTCCGACGATCCAGTATTCTAAGTTCGA7940.20467557374893988No Hit
AGGCGACTTTGTCCCTGAGGTGCGGCGCAAGGGTATGGCCGACAACA7740.1995200177351127No Hit
ACGTGACTCCGCTCGTATCCGACGATCCAGTTCATATTGATCGAACG7690.1982311287316559No Hit
ACGTGACTGCATGCCTAGGTGGAGGCGCCTCAGGGCCACGGGCCACG7580.19539557292405094No Hit
ACGTGACTTGAGGAATGTATTTTTAAGGCATAGATATGCCTATGTGC6960.1794133492811866No Hit
ACGTGACTAGCTTGTAAGGTGGCAGAGCAGACGGCTATGTGCTCGAA6890.1776089046763471No Hit
ACGTGACTGAACGTGAGGTTACTGGAGGGGCTGGTCAGATGGCTCAG5800.14951112440098885No Hit
ACGTGACTTTTTCAGACGATCGAACGTGACTGTAGAACTCTGAACCC5720.14744890199545796No Hit
ACGTGTCTCTTTTTATACCCACGCTAACGCCAGCTTCGGATGCGATA5430.13997334577540851No Hit
ACGTGACTGTCACGACGCAGTTATCTCCATGCAGCAGCACACGAATG5170.13327112295743315No Hit
ACGTGACTTTAAGAGGAGTCCGACGATCCAGTAGAATTGGGTCGTCG4560.11754667711526018No Hit
ACGTGACTAACATTTATCGACGATCCAGTAGAGTTAATTCGAACGTG4320.11136000989866754No Hit
ACGTGACTAAGAATAGCGTCCGACGATCCAGTCGGACTGTAGAACTC4180.1077511206889885No Hit
ACGTCAGTGCACAAGAAGTCAAAAAAATAAGTAAAAGTTGAGAGGGA4110.10594667608414898No Hit
ACGTGACTTCGGACCAGTGAAAATCACGGAAAATGAGAAATACACAT4080.10517334268207491No Hit
ACGTGACTTAGAGAAGAAGTGAGGGAAGATAAATGCACTAAACATAG3950.10182223127308723No Hit
ACGTGACTAACATTTATGCGACGATCCAGTAGAGTTAATTCGAACGT3930.10130667567170451No Hit
ACGTGACTAGTCAACGAAGTCCGACGATCCAGTGGTTCATAGTCGAA3910.10079112007032179No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGAG200.001111033641.0016818
CGTAAGG401.5457772E-841.0016817
CTCACCG304.1033963E-640.996397
ACTGCGG506.002665E-1140.996396
CTCCGGT256.7340676E-540.996397
CTATCGC304.1033963E-640.996397
CCCCGCG352.514007E-740.996399
ACGTGCG256.7340676E-540.996391
CTCGCCC304.1033963E-640.996397
CTTTCGC304.1033963E-640.996397
TTTGCGA352.514007E-740.996398
GTAACTA256.7340676E-540.996393
CTTCGGT459.567884E-1040.9963877
CTTCGCG401.5474143E-840.9963877
TTCACGC200.001111738640.9963878
CTCGCAC401.5474143E-840.9963877
TTCGGAG200.001111738640.9963878
CTAGCGT200.001111738640.9963877
AACGTCC256.739189E-540.99110815
CCGGATT256.739189E-540.99110813
Produced by FastQC (version 0.11.5)