FastQCFastQC Report
Thu 26 May 2016
SRR2099133_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099133_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186500
Sequences flagged as poor quality0
Sequence length47
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTGACTCAAACAGCTTTCTACAGTCCGACGCTCCAGTAATTGGTG7770.4166219839142091No Hit
ACGTGACTCGGAAGAATGTCCGACGATCCAGTTTCCAATGATCGAAC7260.3892761394101877No Hit
ACGTGAGCAGCTATAGCCATTGGTGTCCTTTATCCAGATCAGTATGG6370.3415549597855228No Hit
ACGTGACTTCGGGCTCATCGAACGTGACTTTTTTTTTTTTTTTTTTT6150.3297587131367292No Hit
ACGTGACTAAATCGGGACAGTCCGACGATCCAGTATTCTAAGTTCGA6050.32439678284182305No Hit
ACGTGACTACGAACACCTCCGACGATCCAGTTTGATAGCGTCGAACT5380.28847184986595176No Hit
AGGCGACTTTGTCCCTGAGGTGCGGCGCAAGGGTATGGCCGACAACA5200.27882037533512066No Hit
ACGTGACTCCGCTCGTATCCGACGATCCAGTTCATATTGATCGAACG4870.26112600536193026No Hit
ACGTGACTGCATGCCTAGGTGGAGGCGCCTCAGGGCCACGGGCCACG4790.25683646112600533No Hit
ACGTGACTTGAGGAATGTATTTTTAAGGCATAGATATGCCTATGTGC4430.23753351206434314No Hit
ACGTGACTAGCTTGTAAGGTGGCAGAGCAGACGGCTATGTGCTCGAA4320.2316353887399464No Hit
ACGTGACTTTTTCAGACGATCGAACGTGACTGTAGAACTCTGAACCC3520.18873994638069705No Hit
ACGTGACTGTCACGACGCAGTTATCTCCATGCAGCAGCACACGAATG3460.18552278820375337No Hit
ACGTGTCTCTTTTTATACCCACGCTAACGCCAGCTTCGGATGCGATA3330.17855227882037533No Hit
ACGTGACTGAACGTGAGGTTACTGGAGGGGCTGGTCAGATGGCTCAG3100.16621983914209115No Hit
ACGTGACTTTAAGAGGAGTCCGACGATCCAGTAGAATTGGGTCGTCG2840.1522788203753351No Hit
ACGTGACTAACATTTATCGACGATCCAGTAGAGTTAATTCGAACGTG2750.14745308310991956No Hit
ACGTGACTTCGGACCAGTGAAAATCACGGAAAATGAGAAATACACAT2710.1453083109919571No Hit
ACGTGACTAAGAATAGCGTCCGACGATCCAGTCGGACTGTAGAACTC2680.14369973190348526No Hit
ACGTGACTAGTCAACGAAGTCCGACGATCCAGTGGTTCATAGTCGAA2600.13941018766756033No Hit
ACGTCAGTGCACAAGAAGTCAAAAAAATAAGTAAAAGTTGAGAGGGA2580.1383378016085791No Hit
ACGTGACTACTAGACACGATATATACCCAGTGATGGGATTGCTGGGT2490.13351206434316354No Hit
ACGTGACTAACATTTATGCGACGATCCAGTAGAGTTAATTCGAACGT2440.13083109919571045No Hit
ACGTGACTAGTCGATACGTCCGACGATCCAGTTTAATGAAGTCGGAC2280.12225201072386059No Hit
ACGTGACTATGTGCGCAACATAACTCCAACATCATCAACCTCATACA2170.11635388739946381No Hit
ACGTGACTTAGAGAAGAAGTGAGGGAAGATAAATGCACTAAACATAG2120.11367292225201073No Hit
ACGTGACTTAAATAATGGGAAGAATCAATATTGTAAAAATGGTCATA2110.11313672922252012No Hit
ACGTGACTAAGAGAGAATAATTCGAACGTGACTTTTTTTTTTTTTTT2050.10991957104557641No Hit
ACGTGACTATGGGTTCTAGGAGAGCGGTCCAGCCCCAGGGATCTTCC1910.10241286863270777No Hit
ACGTGACTAGCTTGTAAGTGGCAGAGCAGACGGCTATGTGCTCGAAG1880.10080428954423591No Hit
ACGTGACTAAGAAAAGAATGAATACCTTCTGGTTTTTGTGAACGTTC1870.1002680965147453No Hit
ACGTGACTCTTATTATAGGGAGTTGAGCAGCTTCTGTTGTGATTCTC1870.1002680965147453No Hit
ACGTGACTCGTAGAAAGGATTAAGTCTCGAACGTGACTTAATCACTG1870.1002680965147453No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACG256.6953566E-541.0225417
CGTCTCG200.001106774841.02253717
CGTAAGG352.4921246E-741.02253717
TTGCGGG200.001108237141.0115341
GTGAGGC200.001108237141.0115340
TCGGGCT1050.041.000549
TTCGGGC1050.041.000548
CGGGTAC459.495125E-1041.0005389
ACTATCC352.5026384E-741.0005386
TCGTTAT1250.041.0005388
ATCTCCG256.716581E-541.00053813
GCCCAAT200.001109700941.00053810
ACTGCGC401.5386831E-841.0005386
GTACAAT200.001109700941.0005389
CTTCCGG200.001109700941.0005387
CTATCTA352.5026384E-741.0005387
CAACGCG256.716581E-541.00053810
CCTAGGT352.5026384E-741.00053814
CTATCGC200.001109700941.0005387
CTATCAT256.716581E-541.0005387
Produced by FastQC (version 0.11.5)