FastQCFastQC Report
Thu 26 May 2016
SRR2099132_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099132_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580741
Sequences flagged as poor quality0
Sequence length47
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACTGATAGACCATCAGTTTTCCTTGGCTGGCTTACTTTGCAGAT10940.18838001794259404No Hit
TGCACTGACCCTCGTCCGTGAGTCGTATTACCGGCCGACGATCAGTG8750.15066957559393945No Hit
TTCACCGAGGTACGGCGTGCTGATCCAGGGCGAGACCGGTACCGGCA8360.14395401736746674No Hit
TGCAGTGATGAACGGTACGTATTAGCCAGGATGGTCTCGATCTCCTG7710.1327614203233455No Hit
TGCACTGATGTCAGGGAGGAAGAAAGCTTGCACTGATTTTTTTTTTT7160.12329076128601218No Hit
TGCACTAATGGCCCCACCTAATCTGCAGTATCCTTGCCAAATTGTGC7080.12191321088058187No Hit
TGCACTGAGTCTATTTCAATGCGTGGGCTGACATCTGCTGGCCGAAA6830.11760836586361219No Hit
TTCACTGCAAGTCTGCAAGTTTGGGAATGGATATCTCAGACCCTACA6350.10934306343103035No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCCG200.001111518441.001478
CTGACGT6350.041.0014655
CTAACGG459.567884E-1041.0014657
ACGTCGA304.10344E-641.001468
ATCCGAT200.001112460640.99440415
ACGGCTT200.001112460640.99440414
TTCGGCG401.5505066E-840.99440412
TTCGCTA200.001112460640.99440412
GTCTCGC256.7401445E-540.994412
GTTAACG256.7401445E-540.994414
CCTTTCG256.7401445E-540.994412
CACCGGT256.7401445E-540.994412
TTGGCGC256.7469926E-540.98734311
ATCCGCG256.7469926E-540.98734311
ACTGACG27150.040.3973924
CTGATAT9800.040.1647035
CTCGTCC2400.040.13344211
CACTGAG144500.040.107663
ACTGACC26800.040.083524
CACTGAC107900.040.051473