FastQCFastQC Report
Thu 26 May 2016
SRR2099131_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099131_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301059
Sequences flagged as poor quality0
Sequence length47
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACTGATAGACCATCAGTTTTCCTTGGCTGGCTTACTTTGCAGAT6970.23151608156540743No Hit
TGCAGTGATGAACGGTACGTATTAGCCAGGATGGTCTCGATCTCCTG5420.18003115668357364No Hit
TTCACCGAGGTACGGCGTGCTGATCCAGGGCGAGACCGGTACCGGCA5410.1796989958778844No Hit
TGCACTGACCCTCGTCCGTGAGTCGTATTACCGGCCGACGATCAGTG5290.1757130662096134No Hit
TGCACTGATGTCAGGGAGGAAGAAAGCTTGCACTGATTTTTTTTTTT4900.16275879478773264No Hit
TGCACTAATGGCCCCACCTAATCTGCAGTATCCTTGCCAAATTGTGC4540.15080100578291963No Hit
TTCACTGCAAGTCTGCAAGTTTGGGAATGGATATCTCAGACCCTACA4040.1341929654984571No Hit
TGCACTGAGTCTATTTCAATGCGTGGGCTGACATCTGCTGGCCGAAA3790.12588894535622586No Hit
TGCACTGATCACTGAAACGGGTACAAAAGTCTAAACCCTCAAACTTT3710.12323165891071186No Hit
TGCACTGAGGGAGAGATGTCCGACGATCAGTCTAATGGTAGAGCTCT3390.11260251312865584No Hit
TGCACTGCTAGAGAAAACAAAAGAACTAGCTTGCACTGATTTTTTTG3180.10562713620918159No Hit
TGCACTGATGAACCATCGGGCAAGCTCATGGAGCTGCATGGGGAAGG3030.10064472412384283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAC700.041.00564636
TGTTTCG200.001111015940.99883725
GACAGCG459.549694E-1040.9988377
TCGTTAA200.001111015940.99883712
CGTATGA200.001111015940.99883710
CGTATCC200.001111015940.99883712
CTCAACG401.5446858E-840.9988379
CGGCCAA304.098647E-640.99883716
ACCGCTG200.001111015940.99883712
TGAACGC553.6379788E-1240.9988376
TGTATCG506.002665E-1140.9988379
TGCGGTC352.5103145E-740.99883714
ATATGCG200.001111015940.99883714
TACGGCG750.040.99883711
TGACGCT459.549694E-1040.9988376
ACCCTCG1250.040.9988378
CGTTAAC200.001111015940.99883713
CGCCTAT200.001111015940.9988379
GTTACGC256.7281006E-540.99883710
TGCGCTA200.001111015940.9988376
Produced by FastQC (version 0.11.5)