FastQCFastQC Report
Thu 26 May 2016
SRR2099129_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099129_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131758
Sequences flagged as poor quality0
Sequence length47
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACGGAAACTACAGGGGTGCTGGTGCCCGTGGCCTGGCCGTGTG25421.9292946158867015No Hit
GACTACGTCGACGTCCGCGGCCGACGATCTCGACGTAGTCACTGCGG4910.37265289394192386No Hit
GACTGCGTTAGAGGCAGGATGGCCTGGGTCTTCTTCTCGTCCAGCAG4900.3718939267444861No Hit
GACTACGTCCTATCGAAACGATCTCGAATAGCCACACAGTGACTACG4730.35899148438804473No Hit
GACTACGTTGTCTGCTACAGGTCACCTAGTTTGCAGGACACAACAAT4490.3407762716495393No Hit
GACTCCATCTAAGCCTCGAGACTGTCTCAAAAAAACAAAAAACAAAA4270.32407899330590934No Hit
GACTACGTACGCATAAAGGAAGAGCAGTGACTACGTTTTTTTTTTTT4230.3210431245161584No Hit
GACGACGAACGGCAGCTGTCAACGGGGCTTCGGGTATCGCAGTAGGT4040.3066227477648416No Hit
GACTACGTGCTGGGGCTTATGCTTACAGTAATTGACTGAGAAGCTGT3480.2641205847083289No Hit
GACTACGTTGAGGCGTATCAGGCATGGTGGTGCACACCTGTAGTCCC3470.26336161751089116No Hit
GACTTCGAAAAGTATCTGCGCACGTTGGACGCCGTGGACGCCTGCTT3340.25349504394420075No Hit
GACTACGTGAAAAGGCACGACGATCTCGATCGTAGGACTGTAGAACT3080.23376189681081982No Hit
GACTACGTCCGCACGCTCTACAGTCCGACGATCTCGAACGGAGGGCC3070.2330029296133821No Hit
GACTACGTAGAGGGGGGCAGTCCGACGATCTCGACCACGAAGTCACT3020.2292080936261935No Hit
GACTACGTGAGCGACATCAGTCCGACGATCTCGACGGCTAAGCCAGT2930.22237738884925393No Hit
GACTACGTTACAGCGGGTCCGACGATCTCGAGGGAAACCCCTATAGT2880.2185825528620653No Hit
GGCGACGTGATGCTCACTGAAGACGAGGACGTCTTTGCCGCCTGGCT2880.2185825528620653No Hit
GACTACGGAAACTACAGGGGGTGCTGGTGCCCGTGGCCTGGCCGTGT2790.2117518480851258No Hit
GACTACGTTAATGAACTGTCAAAGATGTCCTGCAGGATGTAAAATGC2650.2011263073209976No Hit
GACTACGTCGACGTCCGCCGGCCGACGATCTCGACGTAGTCACTGCG2630.19960837292612213No Hit
GACTACGTTCGGCGGTCGTGACTACGTTTTTTTACTCGAGATCGGAC2390.1813931601876167No Hit
GACTCCCTAGGCCGGTAGTCCGACGATCGTCGGACTGTAGAACTCTG2380.18063419299017897Illumina DpnII expression Sequencing Primer (100% over 23bp)
GACTACGTTGGGCGTTCGATGCGACGTTTGCGGACTTGGTGGCGCTG2350.1783572913978658No Hit
GACTACGTTTGGGGAACCAGTCCGACGATCTCGAAAAAAGAAGCAGT2170.1646958818439867No Hit
GACTACGTATGGAAGCCGGCTCACGCCTGTAATCCCAGCACTTTGGG2090.1586241442644849No Hit
GACGACGAACGGCAGCTGGTCAACGGGGCTTCGGGTATCGCAGTAGG2090.1586241442644849No Hit
GACTACGTTAGTATGGACAGTCCGACGATCTCGAGCATACCAGCAGT2090.1586241442644849No Hit
GACTACGTGTCTTGGGTGAAAAGGCCAGGGCCCAATGGCTTAACTGC1950.1479986035003567No Hit
GTCTTCGTAGGCCTGGTCCGTGATAGGGGCATTCAAGGTGGCCGTTC1940.14723963630291897No Hit
GACTACGTTGGGCGTTCGGATGCGACGTTTGCGGACTTGGTGGCGCT1930.14648066910548127No Hit
GACTACGTACACCTACCGGAAAAAGCTGGGTGGGAGATGATGAAATA1890.14344480031573034No Hit
GACTACGTACAATCACCCGACGATCTCGAGGCCAGCCGCAGTGACTA1860.14116789872341717No Hit
GACTACGTGGGGGGGGTAGTGACTACGTTTTTTTTTTTTTTTTTTTT1840.1396499643285417No Hit
GACTACGTGAGGAATTTAGTCCGACGATCTCGACAGTGGAACTCTGA1640.12447062037978719No Hit
GACTACGTCGCAATACGACGACTCACTATAGTGAGTCGTATTACCGG1620.12295268598491174No Hit
GACTACGTCGACGTCCGGGCCGACGATCTCGACGTAGTCACTGCGGA1570.11915784999772311No Hit
GACTACGTACAAAACGCCTCGAAAGAAACAACAGTGACTACGTTTTT1570.11915784999772311No Hit
GACTACGTTGCTTTGTTTACAGTCCGACGATCTCGACAGGCCAGTCT1560.11839888280028538No Hit
GACTACGTGACGTTAAACAGTCCGACGATCTCGACTAGAAAGACAGT1540.11688094840540991No Hit
GACTACGTACAAAACGCTCTCGAAAGAAACAACAGTGACTACGTTTT1530.11612198120797218No Hit
GACTACGTTGAGGCGTAGTCAGGCATGGTGGTGCACACCTGTAGTCC1430.10853230923359493No Hit
GACTACGTCCTCAGGTAAAATCGGATAAAACCAATCGTCCTGAACGA1430.10853230923359493No Hit
GACTACGTTCGTGCCTACTTGAACCTGGGAGGTGGAGGTTGCAGTGA1370.10397850604896856No Hit
GACTACCTGATTAGGACGTCCGACGATCTCGACATGCGCCACAGTGA1370.10397850604896856No Hit
GACTACGTTGTCTGCTAAGGTCACCTAGTTTGCAGGACACAACAATC1370.10397850604896856No Hit
GACTACGTCTGTCACTCGCTACGTTTTTTTTTTCGAGATCGTTGTAG1360.10321953885153083No Hit
GACTACGTCGACGTCCGACGATCTCGATGCAAAACCCAGTGAGTCGT1350.1024605716540931No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGCG352.4863584E-741.0189918
GCGGTCC200.001106060141.0189921
CCGGCGG200.001106060141.0189922
GCACGCA200.001106060141.0189924
AGAACCA200.001106060141.0189921
AGACGAG352.4863584E-741.0189921
AAGCACG200.001106060141.0189922
AGACCCA304.0672858E-641.0189921
GGCGGGT200.001106060141.0189924
GACGAGG352.4863584E-741.0189922
AAAGCAC200.001106060141.0189921
AGCACGC200.001106060141.0189923
GTCATCA1200.041.0034187
TTAGGTT352.49378E-741.00341830
CGTGGGG850.041.0034186
CGTGGGC800.041.0034186
CGTGGCA352.49378E-741.0034186
TAGAGGG700.041.0034188
GTCGCGG304.077925E-641.0034187
GTACCGT401.531771E-841.0034187
Produced by FastQC (version 0.11.5)