FastQCFastQC Report
Thu 26 May 2016
SRR2099128_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099128_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324331
Sequences flagged as poor quality0
Sequence length47
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACGATCGGATCTGCAGACTCACTATAGTGAGTCGTATTACCGGCC27200.8386494044664248No Hit
TGACGATCACATCGAAAGGGCGAGCGATCCGGCGCGCCTGCACCACT23610.7279600161563341No Hit
TGACGATCGGATCTGCAAGTGAGTCGTATTACCGGCCGACGATCCAT13290.4097665656381906No Hit
TGACGCGCCAAGCAGGGAGCGCGTCGCCTTCGTGCCCGGCCACCGCA13200.40699162275576495No Hit
TGACGATGTAAACAGCGGCTCGAACCTCGTCGACACCCTGCAGCTGC10540.3249766442307396No Hit
TGACGATCAGGGCGAGAGGGAGGCAGAGGTTGCAGTGAGCCAAGATT9410.2901356947069506No Hit
TGACGGACGAAATAATGGCCGACGAGGCCGTTGTCGCCAAGCACGCC9280.2861274438767802No Hit
TGACCATTACGGCGAGTCCCGGGCACCTCAGCCCCAAAACACATCAG8300.25591139915703404No Hit
TGACGATCAGGGGGTAACAGTCCGACGATCCATGCACATTGGATCGT7670.2364867989800543No Hit
TGACGATGTAAACAGCGGGCTCGAACCTCGTCGACACCCTGCAGCTG5720.17636303652749816No Hit
TGACGATCAACGAAATTGTCCGACGATCCATGATAGTCGGGCTGTAG5670.1748214015928172No Hit
TGCCGAGCTCGCTATGACGTCGATCGGGCGCACTTCGTCGTGCACCG5400.1664965729455402No Hit
TAACTATCTATGTCGGTGAAAAAGTGTCTGCCGCTGTGATCGTTTGC5210.1606383601937527No Hit
TGACGATCACATGGAACGGGAATTATCTTCTCCTGCTGTCTGGTGAA5140.15848007128519936No Hit
TGACGATCGTCTTAAGCCCGACGATCCATGCTACCTAAGTCAGTGAC5020.15478014744196514No Hit
TGACGATCGTCACCCAGCAGTCCGACGATCCATGTAAACAAGCTCAG4870.15015524263792238No Hit
TGACGATCTAGGCAGCTGTTTAGGGCATACAGAATACCAGAGAGGGG4860.14984691565098618No Hit
TGACGATCAACGAGGGGGGAAAGTAATTAAGGTTCAGTGAAGTCTTA4830.14892193469017764No Hit
CGGCGATCCTGCCGGTGGTCGACGTTGTTCATGTACGTCTCGTTGAA4520.13936379809515587No Hit
TGACCATTACGGCGAGTTCCCGGGCACCTCAGCCCCAAAACACATCA4500.1387471441212835No Hit
TGATGATCCAGAAGGCTCGTCAGGTTGCTTCGCGCCCACGTCAGGAT4390.13535554726498547No Hit
TGACGATCTATTCGAAATCCGACGATCCATGGCAAACTTGTCAGTGA4380.13504722027804927No Hit
TGACGATCAATTAGCCCACGATCCATGTAGTGAGTCGTATTACCGGC4280.13196395040868744No Hit
TGATGATCCAGAAGGCTTCGTCAGGTTGCTTCGCGCCCACGTCAGGA4220.13011398848707031No Hit
TGACCATTACGGCGAGTGCCCGGGCACCTCAGCCCCAAAACACATCA4140.12764737259158082No Hit
TGACGATCGATAATTCACTACAGTCCGACGATCCAGAATGTAGAACT4110.12672239163077226No Hit
TGACGATCGCTACTCACCTACAGTCCGACGATCGTCAACGAGGACGG3930.12117250586592093No Hit
TGACGATCTAGGCAGCTGGTTTAGGGCATACAGAATACCAGAGAGGG3870.11932254394430382No Hit
TGACGATCAAATCCCACCAGTCCGACGATCCATGGGTCGTCGGACTG3820.11778090900962288No Hit
CGACGATCCGCAAACGGTGGGGCTCCGTGATCGCCTTGGCGTCCGCG3760.11593094708800576No Hit
TGATGATCAAGATTTGTGTTGTGGAAACACAACCAACCTTGCGAGTA3610.111306042283963No Hit
TGACGATCCCAGCATCAGTCCGACGATCCATGGGAGAGCTCTGAACC3510.10822277241460113No Hit
TGACGATCACATCGAAAGGGGCGAGCGATCCGGCGCGCCTGCACCAC3360.10359786761055835No Hit
TGACGATCTGGTAGAATGTCCGACGATCCATGATGGACACCTCAGTG3330.10267288664974979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGCGC1250.041.0118816
TACGGTA200.001109404841.0118816
GTCCCGG1000.041.0118816
CTCTAGA304.0906707E-641.0118816
GTTACCA200.001110247241.00555414
GCCCTTA200.001110247241.00555413
CGGTGGT505.820766E-1141.00555413
CGTACAC352.5078953E-741.00555415
CAAGCGG304.095011E-641.00555413
CAGCTTA200.001110247241.00555413
CCGTTTG200.001110247241.00555414
TTCTCGA256.7227505E-541.00555414
GCCCATA200.001111090140.9992312
TAGCGGG459.549694E-1040.9992312
TACGGAG200.001111090140.9992312
TAGCCCA352.510933E-740.9992312
TGCGCGC200.001111090140.9992312
TAACAGG256.7288696E-540.9992312
ACCCCGG459.549694E-1040.9992312
TAGCTCC200.001111090140.9992312
Produced by FastQC (version 0.11.5)