FastQCFastQC Report
Thu 26 May 2016
SRR2099127_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099127_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171086
Sequences flagged as poor quality0
Sequence length47
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACGATCGGATCTGCAGACTCACTATAGTGAGTCGTATTACCGGCC26951.5752311702886268No Hit
TGACGATCACATCGAAAGGGCGAGCGATCCGGCGCGCCTGCACCACT15520.9071461136504447No Hit
TGACGATCGGATCTGCAAGTGAGTCGTATTACCGGCCGACGATCCAT13100.7656967840735069No Hit
TGACGCGCCAAGCAGGGAGCGCGTCGCCTTCGTGCCCGGCCACCGCA8510.4974106589668354No Hit
TGACGATGTAAACAGCGGCTCGAACCTCGTCGACACCCTGCAGCTGC6910.4038904410647277No Hit
TGACGATCAGGGCGAGAGGGAGGCAGAGGTTGCAGTGAGCCAAGATT6300.3682358579895491No Hit
TGACGGACGAAATAATGGCCGACGAGGCCGTTGTCGCCAAGCACGCC6120.357714833475562No Hit
TGACCATTACGGCGAGTCCCGGGCACCTCAGCCCCAAAACACATCAG5640.32965876810492967No Hit
TGACGATCAGGGGGTAACAGTCCGACGATCCATGCACATTGGATCGT5110.2986801959248565No Hit
TGACGATGTAAACAGCGGGCTCGAACCTCGTCGACACCCTGCAGCTG3880.22678652841261118No Hit
TGACGATCAACGAAATTGTCCGACGATCCATGATAGTCGGGCTGTAG3690.21568100253673592No Hit
TGCCGAGCTCGCTATGACGTCGATCGGGCGCACTTCGTCGTGCACCG3640.21275849572729508No Hit
TAACTATCTATGTCGGTGAAAAAGTGTCTGCCGCTGTGATCGTTTGC3330.19463895350876168No Hit
TGACGATCTAGGCAGCTGTTTAGGGCATACAGAATACCAGAGAGGGG3240.18937844125176811No Hit
TGACGATCTATTCGAAATCCGACGATCCATGGCAAACTTGTCAGTGA3230.18879393988987994No Hit
TGACGATCGTCTTAAGCCCGACGATCCATGCTACCTAAGTCAGTGAC3120.18236442490911003No Hit
TGACGATCAATTAGCCCACGATCCATGTAGTGAGTCGTATTACCGGC3110.18177992354722186No Hit
TGACGATCAACGAGGGGGGAAAGTAATTAAGGTTCAGTGAAGTCTTA3090.18061092082344551No Hit
TGACCATTACGGCGAGTTCCCGGGCACCTCAGCCCCAAAACACATCA3070.1794419180996692No Hit
TGACGATCGTCACCCAGCAGTCCGACGATCCATGTAAACAAGCTCAG3050.17827291537589282No Hit
TGACGATCACATGGAACGGGAATTATCTTCTCCTGCTGTCTGGTGAA3050.17827291537589282No Hit
CGGCGATCCTGCCGGTGGTCGACGTTGTTCATGTACGTCTCGTTGAA2980.17418140584267564No Hit
TGACCATTACGGCGAGTGCCCGGGCACCTCAGCCCCAAAACACATCA2860.16716738950001753No Hit
TGATGATCCAGAAGGCTCGTCAGGTTGCTTCGCGCCCACGTCAGGAT2840.16599838677624118No Hit
TGATGATCCAGAAGGCTTCGTCAGGTTGCTTCGCGCCCACGTCAGGA2800.16366038132868851No Hit
TGACGATCGATAATTCACTACAGTCCGACGATCCAGAATGTAGAACT2670.15606186362414223No Hit
TGACGATCTAGGCAGCTGGTTTAGGGCATACAGAATACCAGAGAGGG2660.15547736226225406No Hit
CGACGATCCGCAAACGGTGGGGCTCCGTGATCGCCTTGGCGTCCGCG2530.14787884455770783No Hit
TGACGATCGCTACTCACCTACAGTCCGACGATCGTCAACGAGGACGG2380.1391113241293852No Hit
TGACGATCAAATCCCACCAGTCCGACGATCCATGGGTCGTCGGACTG2340.1367733186818325No Hit
TGACGATCTGGTAGAATGTCCGACGATCCATGATGGACACCTCAGTG2330.13618881731994437No Hit
TGACGATCACATCGAAAGGGGCGAGCGATCCGGCGCGCCTGCACCAC2270.1326818091486153No Hit
TGACGATCCCAGCATCAGTCCGACGATCCATGGGAGAGCTCTGAACC2260.13209730778672715No Hit
TGATGATCAAGATTTGTGTTGTGGAAACACAACCAACCTTGCGAGTA2120.12391428872029271No Hit
TGACGATCAGGGCGAGAGGGGAGGCAGAGGTTGCAGTGAGCCAAGAT2050.1198227791870755No Hit
TGACGCTCCATAGATCGGTTAGAGCGTCAGTGACGATCTAACCATGG2030.11865377646329915No Hit
TGATGATCCAGAAGGCTACGTCAGGTTGCTTCGCGCCCACGTCAGGA2010.11748477373952282No Hit
TGACGATCCCATTAAAGATTAGGCCGGTAATCGATCGTCACTGACTT1990.11631577101574647No Hit
TGACGTTGTGCCGCAGGCCCGTCTGCCCGAAGCTAGCCGCAACCCCA1820.10637924786364754No Hit
TGACGATCTGCGCTGTTGAACACTTGAGGTCAGGAGTTTGAGACCAG1740.10170323696854214No Hit
TGACGATCAATATGCAAAGTCCGACGATCCATGTGGGTTTGTAGAAC1720.10053423424476579No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTCGT200.001105548141.02984218
AAGTGAG1750.041.02984217
GAGTCCC553.6379788E-1241.01784514
AACGGTG304.0752293E-641.01784513
CGGTGGT304.0752293E-641.01784513
ACGGTGG304.0752293E-641.01784514
GACAGCC200.00110714141.0178414
CAAGTAC200.00110714141.0178413
CGACGTG352.492743E-741.0178414
TCTCTAG352.492743E-741.0178415
GTCTCTA352.492743E-741.0178414
AAAGTCC200.00110714141.0178416
ACCTACA401.531771E-841.0178416
GTTGTTG200.00110714141.0178416
AGATTAG352.492743E-741.0178416
TGCTCTC200.00110714141.0178413
TTAGTCC200.00110714141.0178416
CCGGTTC200.00110714141.0178415
ACCGTTT200.00110714141.0178413
GTCCCGG401.531771E-841.0178416
Produced by FastQC (version 0.11.5)