FastQCFastQC Report
Thu 26 May 2016
SRR2099126_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099126_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275757
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGTCATACGTGGCGGCGCGCTGGTCCGCCGTTTCCTCGAA18730.6792211983739307No Hit
GAACGGCAGTCAGATCGGGAAGCGGCCCTGCAGTTCGGGAATC13440.48738563300297No Hit
GTACGTCACCCGACTGAAAAACACTGTGCTATATTCTGCAATA10470.3796821114241887No Hit
GCACGTCACGCGGGGGGGACGCACCCTACCGACGGTAAACCCC10320.37424253962728055No Hit
GTACGTCATTGGACAGAGATTCAGCTGACCCCATGTGGCCACC9140.3314512414916031No Hit
GTACGGCGGGGGGTGCTGATCCAGGGCGAGACCGGTACCGGCA8500.308242401824795No Hit
GTACGTCACCGGCCCGCGACGCCGCCCAGCGCCAGCCGCCACT7000.2538466838557135No Hit
GTACGTCATTGTTGGCAAAGGGGTCGTGGTAGTTATGAAACGC6660.24151698778272176No Hit
GCACGTCAGTATCCAGACGCTCTCTGGTGTGAGCGTCTGTGGG6610.23970379718375234No Hit
GCACGTCAGTATCTCTCTGGTGTGAGCGTCTGTGGGTGTGAAA6450.23390158726705035No Hit
GTACGTCAAAGCCAGGAAGAAGAAGTCTTACACCACTCCCAAG5740.2081542807616851No Hit
GTACGTCAGCAGAGGACACGGAGTCATCACGATGTGAGGGAAC4760.1726157450218852No Hit
GAACGGCAGTCAGGATCGGGAAGCGGCCCTGCAGTTCGGGAAT4000.14505524791755062No Hit
GTACGTCAGCCCAGGGAATGAGGAAGGAGACAGCAGGACTGTC3990.14469260979775672No Hit
GTACGTTAATTCCCACACATCACAAAGAAGTTTCTGAGAATGC3940.14287941919878736No Hit
GTACGTCAATTCAGCTCATAAAGGAACCTAAGCAAGCTGTATT3810.13816512364146696No Hit
GTACGTCATGGCCCCAGTCCGACGATCCTGCGGTACGTCGGAC3750.1359892949227037No Hit
GTACGTCAACCGAGAGTCATCCACAGCCTCTGCTCTAGTGGCA3700.1341761043237343No Hit
GTACGTCATCAACGGCTGAGAAAGGCACAGAGGCTGCTGCTGC3630.13163763748517718No Hit
GTACGTCAATAGCCAGTCCGACGATCTTTTGGTACGTCGGACT3580.12982444688620778No Hit
GTACGTCGCACTGCTACAGTCCGACGATCACCACGTACGTTTG3580.12982444688620778No Hit
GTACGTCAAAGACCAGTCCGACGATCGGAACGTACGCTTAGAT3560.12909917064662002No Hit
GTACGTCAGAAAAGGAGAAGGGGAAGACCAGAGCAAAGACTCT3560.12909917064662002No Hit
GTACGTCAGTCATGTCAGCTTAGCGATAGTCCGGTTCTGGCAA3530.12801125628723842No Hit
GTACGTCATGAAGTACTGAGCAGGAAGGCCTCCCCTGCCTCTC3500.12692334192785676No Hit
GTACGTCAGCCTCGGTGGATTTGGAGGAAGAGGCCGGGGAGGC3380.12257168449033025No Hit
GTACGTCAAACAGATCATCTGCATGTGTGTACAGATGATTGGA3340.12112113201115475No Hit
GTACGTCACGAAAGTCAAAGATGTCCTGCAGGATGTAAAATGC3310.1200332176517731No Hit
ATACGTAAGTACCATCGTACTGTACTCTCGTCAACTGCTATAG3180.11531892209445271No Hit
GTACGTCACGTGCGGGATAAAACACAGTAGAATTCAGTCTTTA3120.11314309337568947No Hit
GTACGTCAGACTGTCATGTAATTAATTGCATTAATTACAGTCA3030.10987935029754459No Hit
GCCCGTCATACGTGGGCGGCGCGCTGGTCCGCCGTTTCCTCGA2990.10842879781836907No Hit
GAACGGCAGTCAGTATCGGGAAGCGGCCCTGCAGTTCGGGAAT2990.10842879781836907No Hit
GTACGTCATCGACGCGTACGTCATTTTTTTTTTTTTTTTTTTT2880.10443977850063642No Hit
GTACGTCAGTCATGGTCAGCTTAGCGATAGTCCGGTTCTGGCA2860.10371450226104868No Hit
GAACGGCAGTCAGCATCGGGAAGCGGCCCTGCAGTTCGGGAAT2800.10153867354228542No Hit
GTACGTCACGTACTTCTACAGTCCGACGATCAAGGGGTACGTT2800.10153867354228542No Hit
GTACGTCAATGGGAAAAAACAGAAAATAGCTAGTGTTGGCAGG2780.10081339730269767No Hit
GTACGCCATAGAAAGTAATTTTATTCAGAATTTTGTGTTTGTT2770.10045075918290378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGGG403.7071004E-837.1208636
ACAACGG200.001833049637.026518
AGCCGGT200.001833049637.026518
ACAGTAT200.001833049637.026518
ACAAACG251.2260117E-437.026518
AACCACC200.001833049637.026518
AGTGCTC251.2260117E-437.026518
TGCGCCT251.2260117E-437.026519
GTGCTCT200.001833049637.026519
AACATAG251.2260117E-437.026518
TTGCGCG403.7913196E-837.026518
ACCCGAT200.001833049637.026518
GCTCGCA251.2260117E-437.026519
ACATACG251.2260117E-437.026518
TCGACGG200.001833049637.026519
ACGACTA251.2260117E-437.026518
TCCGGGT308.248422E-637.0265059
CATTGGA1200.037.0197877
CACGATT200.001834684937.0197877
CATTCGG200.001834684937.0197877
Produced by FastQC (version 0.11.5)