FastQCFastQC Report
Thu 26 May 2016
SRR2099125_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099125_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189175
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGTCATACGTGGCGGCGCGCTGGTCCGCCGTTTCCTCGAA11860.6269327342407823No Hit
GAACGGCAGTCAGATCGGGAAGCGGCCCTGCAGTTCGGGAATC8820.46623496762257166No Hit
GCACGTCACGCGGGGGGGACGCACCCTACCGACGGTAAACCCC6960.36791330778379805No Hit
GTACGTCACCCGACTGAAAAACACTGTGCTATATTCTGCAATA6600.3488833091053258No Hit
GTACGTCATTGGACAGAGATTCAGCTGACCCCATGTGGCCACC5930.31346636712039117No Hit
GTACGGCGGGGGGTGCTGATCCAGGGCGAGACCGGTACCGGCA5480.28967886877230076No Hit
GTACGTCACCGGCCCGCGACGCCGCCCAGCGCCAGCCGCCACT4780.2526760935641602No Hit
GTACGTCATTGTTGGCAAAGGGGTCGTGGTAGTTATGAAACGC4700.2484472049689441No Hit
GCACGTCAGTATCCAGACGCTCTCTGGTGTGAGCGTCTGTGGG4440.23470331703449188No Hit
GCACGTCAGTATCTCTCTGGTGTGAGCGTCTGTGGGTGTGAAA4260.22518831769525574No Hit
GTACGTCAAAGCCAGGAAGAAGAAGTCTTACACCACTCCCAAG4020.21250165190960751No Hit
GTACGTCAGCCCAGGGAATGAGGAAGGAGACAGCAGGACTGTC3070.1622835998414167No Hit
GTACGTCAGCAGAGGACACGGAGTCATCACGATGTGAGGGAAC2890.15276860050218052No Hit
GTACGTTAATTCCCACACATCACAAAGAAGTTTCTGAGAATGC2800.14801110083256244No Hit
GTACGTCAATTCAGCTCATAAAGGAACCTAAGCAAGCTGTATT2670.14113915686533632No Hit
GTACGTCACGAAAGTCAAAGATGTCCTGCAGGATGTAAAATGC2640.1395533236421303No Hit
GAACGGCAGTCAGGATCGGGAAGCGGCCCTGCAGTTCGGGAAT2520.1332099907493062No Hit
GTACGTCAACCGAGAGTCATCCACAGCCTCTGCTCTAGTGGCA2470.13056693537729616No Hit
GTACGTCATCAACGGCTGAGAAAGGCACAGAGGCTGCTGCTGC2420.1279238800052861No Hit
GTACGTCAGTCATGTCAGCTTAGCGATAGTCCGGTTCTGGCAA2360.12475221355887406No Hit
GTACGTCAATAGCCAGTCCGACGATCTTTTGGTACGTCGGACT2350.12422360248447205No Hit
GTACGTCAGAAAAGGAGAAGGGGAAGACCAGAGCAAAGACTCT2300.12158054711246201No Hit
GTACGTCATGGCCCCAGTCCGACGATCCTGCGGTACGTCGGAC2270.11999471388925598No Hit
GTACGTCATGAAGTACTGAGCAGGAAGGCCTCCCCTGCCTCTC2230.11788026959164795No Hit
GTACGTCAAAGACCAGTCCGACGATCGGAACGTACGCTTAGAT2220.11735165851724594No Hit
GTACGTCAGACTGTCATGTAATTAATTGCATTAATTACAGTCA2190.1157658252940399No Hit
GTACGTCAAACAGATCATCTGCATGTGTGTACAGATGATTGGA2070.10942249240121579No Hit
GTACGTCAAACGTGAAAAAGCTGGGTGGGAGATGATGAAATAG2050.1083652702524118No Hit
GTACGTCACGTGCGGGATAAAACACAGTAGAATTCAGTCTTTA2030.10730804810360776No Hit
GCCCGTCATACGTGGGCGGCGCGCTGGTCCGCCGTTTCCTCGA2020.10677943702920575No Hit
GAACGGCAGTCAGTATCGGGAAGCGGCCCTGCAGTTCGGGAAT2020.10677943702920575No Hit
ATACGTAAGTACCATCGTACTGTACTCTCGTCAACTGCTATAG1950.1030791595083917No Hit
GTACGTCGCACTGCTACAGTCCGACGATCACCACGTACGTTTG1950.1030791595083917No Hit
GTACGTCAGCCTCGGTGGATTTGGAGGAAGAGGCCGGGGAGGC1930.10202193735958769No Hit
GTACGTCAGTCATGGTCAGCTTAGCGATAGTCCGGTTCTGGCA1930.10202193735958769No Hit
GTACGTCACCAGCGAAAGATGAGGCAGAGGTCCAAGTAAGCCA1920.10149332628518568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG1250.037.0230186
TCTTGCG452.579327E-937.0230186
TCACCGT850.037.0230186
ATGGCCC355.570964E-737.0230188
ACGCAGG355.570964E-737.0230188
TGGACGG355.570964E-737.0230187
CATTGGA900.037.0230187
TGAGTGC600.037.0230186
TCAGGCA850.037.0230186
TCAATTC1200.037.0230186
TCAATTA452.579327E-937.0230186
CTTGCGC452.579327E-937.0230187
GTCACGT2500.037.0230185
TCACTCG355.570964E-737.0230186
TCATCGG1050.037.0230186
TCATCGC850.037.0230186
AGCCTCG355.570964E-737.0230188
CAATAGC900.037.0230187
CACCCGG308.24004E-637.0230187
TCACGTG900.037.0230186
Produced by FastQC (version 0.11.5)