FastQCFastQC Report
Thu 26 May 2016
SRR2099124_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099124_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219342
Sequences flagged as poor quality0
Sequence length42
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCTCAAAAACGACGATCAAAAAATGAGCATCTTTTGATC19200.8753453510955495No Hit
GATGCTCACGACCGACTCACTATAGGGAGTCGTATTACCGGC15000.6838635555433981No Hit
GATGCACAGCCTGTTGGCCGACCAAGCGCACGCGGCAGGTGT13760.6273308349518104No Hit
GATGCTGCCTTGAGCGGGTTTGGCCTTCCAGGCCGCCGCCTG12370.5639594788047888No Hit
GATGCTCATGTCACCTGATCCACGTGGATCGACGTCGGCCCG11190.5101622124353749No Hit
GATGCTCACTCCGGCAGAGTTTCACTGGCCAAATAGAGCCCA9020.41122995140009666No Hit
GATGCTCAGTGCTATGAGTTGGCTCTGTGCCTTGGTCCTATA6380.2908699656244586No Hit
GATACTCAAGACGGCTGGTCGTTCCGGCGACAGCACGAACAC5980.27263360414330134No Hit
GGTGCTCGGACGGCACCACGAGGTTGGCCGTCGGGAATCCGA5700.25986815110649125No Hit
GATGCTCATTTGAGGTATGAAGGAGAACGCCGGAACTCTCAC5510.25120587940294153No Hit
GATACTCAAGACCTGGCTCCTCGCCCCGGAGCCCCAGCATCG5370.24482315288453646No Hit
GATGCCCCGTTCGAACAGCTCGATCCGCTCGTAGGTGGTGAA5190.2366167902180157No Hit
GATGCTCATCAGTGAAGACGAGGACGTCTTTGCCGCCTGGCT4520.20607088473707727No Hit
GATGCTCAGAAGCAGTCCGACGATCCAAAGATGCTCATTTTT4450.20287952147787472No Hit
GATGCTCATCCCAGTATCCGCCCGCCGGCGCCGGCGCAAACC4400.20059997629273008No Hit
GAGGCTGATCAGGAATTCTGAAATACATGAATTCTTTTCCTT4330.19740861303352755No Hit
GATACTCAAGACGCTGGCTCCTCGCCCCGGAGCCCCAGCATC4320.1969527039964986No Hit
GATGCTCACGGCCTACAGTCCGACGATCGTCGGACTGTAGAA4290.19558497688541182No Hit
GATGCCCAGGTCATAACATTGCGCAAGCCTCGTCAACAACGT4230.19284952266323824No Hit
GCTACTCACATGCTACAGTCCGACGATCGTCAACGAGGACGG4020.18327543288563067No Hit
GATGCTCAATCCGCAAGCTGCGCCGCTCGCCGGCGCGCCGGA3760.17142179792287843No Hit
GATGCTCAGACTAAGGAAGGAAGGAAGGAAGGAAGGAAGGAA3750.17096588888584952No Hit
GACGCTCCGCGTCGACGCCGTGGTTGCCGTGAGCGATGCGGT3720.1695981617747627No Hit
GATGCTCAATCCAGCTGCGCCGCTCGCCGGCGCGCCGGATTG3660.1668627075525891No Hit
GATGCGCAGCATCGCATTCTGAAGAACGTCTTCGTCCCCCAG3440.1568327087379526No Hit
GATGCTCATCGTAAGAAAGGAAAGAAGGTGGATGCCAAGAAG3430.15637679970092366No Hit
GATGCTCGGGTCTGCGTCAGGTCGAAGCCGGCACCCAGCAGG3390.15455316355280796No Hit
GATGCTCACTCCGGGCAGAGTTTCACTGGCCAAATAGAGCCC3370.15364134547875008No Hit
GATACTCAAGACAAACCGCCTCCCGCTTGGCTGGCTCCTCGC3220.1468027099233161No Hit
GATGCCCATGTCAGGAGGCGGAGGTTGCAGTGAGTTGAGATT3210.14634680088628718No Hit
GATGCTCAGACTGGAAGGAAGGAAGGAAGGAAGGAAGGAAGG3110.14178771051599784No Hit
GATGCTCAGTCTCAGTCCGACGATCTGTTGATCGTCGGACTG3000.1367727111086796No Hit
GATGCTCACCTGACAGTCCGACGATCACTAGATGCTCATCAG2810.1281104394051299No Hit
GATGCTCATCACATGCTCATTTTTAGATCGTCGGACTGTAGA2720.12400725807186949No Hit
GATACTCAAGACGGGCTGGTCGTTCCGGCGACAGCACGAACA2630.1199040767386091No Hit
TATGCGCAGGGCCCGTTCGACATGGGCCGGGCCATAGCGCCG2580.11762453155346445No Hit
GATGCTCACCCAGTTCTACAGTCCGACGATCATCGGACTGTA2560.1167127134794066Illumina Small RNA Adapter 1 (95% over 21bp)
GATGCTCAATCTAACAAAGAGGAGACGAAGAAAGAAAACAGC2490.11352135022020407No Hit
GATGCTCAAGTTTTCCGACGATCGCTGGACTGTAGAACTCTG2460.11215362310911726No Hit
CATGCACATACGCTGAAGCCTTATGAAATGCAGGTCACCACT2440.11124180503505941No Hit
GATGCTCGGTTGGGCACACTCCGGTGTAGGAGCCCCGGCGCT2400.10941816888694368No Hit
GATGCTCAAAAATCATTCGACGATCAAAAAATGAGCATCTTT2370.10805044177585688No Hit
GATGCTCAAGCGCTACAGTCCGACGATCTGTAGATCTCTGAA2270.10349135140556756No Hit
GATGCTCACTGCCAAAGAAGACCTCCCACTAGGAAGAAAGAG2260.10303544236853864No Hit
GATGCTCATGACTTCTACAGTCCGACGATCATTCGATGGACT2250.1025795333315097Illumina Small RNA Adapter 1 (95% over 21bp)
GATGCTCAGCCCAAAAGACCGGGAAAAACCCCAAGCCTTATT2250.1025795333315097No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGCT453.3051037E-936.10224536
TAGCGCC200.002074311736.1022436
TATCAAG200.002100063736.01163512
CGTGCAA200.002100063736.01163512
CGTCTGT251.4428225E-436.01163512
CTACGAG200.002100063736.01163513
TCACGGA404.8441507E-836.003426
TCACGCT404.8441507E-836.003426
TCACGAA251.4447607E-436.003426
TCTGCGT356.9393536E-736.0034211
CTTCGGT601.8189894E-1236.003425
CAGCGTT356.9393536E-736.003427
CGTGGCG404.8441507E-836.0034210
CAGCGCT356.9393536E-736.003427
AGCCGGA309.986321E-636.003428
AATCCGC453.3942342E-936.003428
GCCCATG1050.036.003424
GCCTTGA1200.036.003427
ACCGGGT200.002102417436.003428
TCACCTA309.986321E-636.003426
Produced by FastQC (version 0.11.5)