FastQCFastQC Report
Thu 26 May 2016
SRR2099123_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099123_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143704
Sequences flagged as poor quality0
Sequence length42
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCTCAAAAACGACGATCAAAAAATGAGCATCTTTTGATC11720.815565328731281No Hit
GATGCTCACGACCGACTCACTATAGGGAGTCGTATTACCGGC9460.6582976117575016No Hit
GATGCACAGCCTGTTGGCCGACCAAGCGCACGCGGCAGGTGT9140.6360296164337805No Hit
GATGCTGCCTTGAGCGGGTTTGGCCTTCCAGGCCGCCGCCTG7910.550437009408228No Hit
GATGCTCATGTCACCTGATCCACGTGGATCGACGTCGGCCCG7380.5135556421533152No Hit
GATGCTCACTCCGGCAGAGTTTCACTGGCCAAATAGAGCCCA5970.41543728775816957No Hit
GATGCTCAGTGCTATGAGTTGGCTCTGTGCCTTGGTCCTATA4280.2978344374547681No Hit
GGTGCTCGGACGGCACCACGAGGTTGGCCGTCGGGAATCCGA3870.2693035684462506No Hit
GATACTCAAGACGGCTGGTCGTTCCGGCGACAGCACGAACAC3860.26860769359238434No Hit
GATGCTCATTTGAGGTATGAAGGAGAACGCCGGAACTCTCAC3760.26164894505372155No Hit
GATGCCCCGTTCGAACAGCTCGATCCGCTCGTAGGTGGTGAA3520.2449479485609308No Hit
GATACTCAAGACCTGGCTCCTCGCCCCGGAGCCCCAGCATCG3470.2414685742915994No Hit
GATACTCAAGACGCTGGCTCCTCGCCCCGGAGCCCCAGCATC3100.21572120469854703No Hit
GATGCTCATCCCAGTATCCGCCCGCCGGCGCCGGCGCAAACC3030.21085008072148306No Hit
GATGCTCATCGTAAGAAAGGAAAGAAGGTGGATGCCAAGAAG3030.21085008072148306No Hit
GATGCTCAGACTAAGGAAGGAAGGAAGGAAGGAAGGAAGGAA2990.20806658130601793No Hit
GAGGCTGATCAGGAATTCTGAAATACATGAATTCTTTTCCTT2910.2024995824750877No Hit
GATGCTCATCAGTGAAGACGAGGACGTCTTTGCCGCCTGGCT2890.20110783276735514No Hit
GATGCTCACGGCCTACAGTCCGACGATCGTCGGACTGTAGAA2800.19484495908255858No Hit
GATGCTCATCACATGCTCATTTTTAGATCGTCGGACTGTAGA2700.1878862105438958No Hit
GATGCTCAGACTGGAAGGAAGGAAGGAAGGAAGGAAGGAAGG2590.1802315871513667No Hit
GATGCTCAGAAGCAGTCCGACGATCCAAAGATGCTCATTTTT2560.17814396258976786No Hit
GATGCCCAGGTCATAACATTGCGCAAGCCTCGTCAACAACGT2520.17536046317430273No Hit
GCTACTCACATGCTACAGTCCGACGATCGTCAACGAGGACGG2410.16770583978177364No Hit
GATGCTCAATCCGCAAGCTGCGCCGCTCGCCGGCGCGCCGGA2410.16770583978177364No Hit
GACGCTCCGCGTCGACGCCGTGGTTGCCGTGAGCGATGCGGT2280.158659466681512No Hit
GATGCTCACTCCGGGCAGAGTTTCACTGGCCAAATAGAGCCC2270.15796359182764572No Hit
GATACTCAAGACAAACCGCCTCCCGCTTGGCTGGCTCCTCGC2270.15796359182764572No Hit
GATGCTCGGGTCTGCGTCAGGTCGAAGCCGGCACCCAGCAGG2260.15726771697377942No Hit
GATGCTCAATCCAGCTGCGCCGCTCGCCGGCGCGCCGGATTG2260.15726771697377942No Hit
GATGCGCAGCATCGCATTCTGAAGAACGTCTTCGTCCCCCAG2220.15448421755831432No Hit
GATGCCCATGTCAGGAGGCGGAGGTTGCAGTGAGTTGAGATT2010.13987084562712243No Hit
GATGCTCAGTCTCAGTCCGACGATCTGTTGATCGTCGGACTG1940.13499972165005844No Hit
GATGCTCAGCCCAAAAGACCGGGAAAAACCCCAAGCCTTATT1930.13430384679619217No Hit
GATACTCAAGACGGGCTGGTCGTTCCGGCGACAGCACGAACA1810.12595334854979678No Hit
GATGCTCACCCAGTTCTACAGTCCGACGATCATCGGACTGTA1690.11760285030340144Illumina Small RNA Adapter 1 (95% over 21bp)
GATGCTCAATCTAACAAAGAGGAGACGAAGAAAGAAAACAGC1650.11481935088793632No Hit
GATGCTCACCTGACAGTCCGACGATCACTAGATGCTCATCAG1640.11412347603407004No Hit
TATGCGCAGGGCCCGTTCGACATGGGCCGGGCCATAGCGCCG1640.11412347603407004No Hit
GATGCTCAAGTTTTCCGACGATCGCTGGACTGTAGAACTCTG1620.11273172632633748No Hit
GATGCTCACTGCCAAAGAAGACCTCCCACTAGGAAGAAAGAG1570.10925235205700608No Hit
CATGCACATACGCTGAAGCCTTATGAAATGCAGGTCACCACT1570.10925235205700608No Hit
GACGCTCCGCGTGGCGACGCCGTGGTTGCCGTGAGCGATGCG1550.10786060234927351No Hit
GATGCTCATCAGGTAAGAAGCAGCCAGTTGCATTCTTTTGGA1520.10577297778767467No Hit
GATGCTCATTGGGGAAAGAAGGTGGATGCCAAGAAGCAGAAG1520.10577297778767467No Hit
GATGCTCATGACTTCTACAGTCCGACGATCATTCGATGGACT1490.10368535322607582Illumina Small RNA Adapter 1 (95% over 21bp)
GATGCTCGGTTGGGCACACTCCGGTGTAGGAGCCCCGGCGCT1470.10229360351834327No Hit
GATGCTCAAGCGCTACAGTCCGACGATCTGTAGATCTCTGAA1450.1009018538106107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAG356.863793E-736.0390313
TTAAGAT200.002089840636.03902413
CGACTTG251.4339147E-436.03902413
CATAACA309.9187055E-636.02647412
CGGCAGA850.036.0264712
GTCTAAG200.002093419236.0264712
TGTTGGC1100.036.0264712
GAGGTAT453.3560354E-936.0264712
CTTCTAC200.002093419236.0264712
TTACGAC251.4368583E-436.0264712
GGTTCTA200.002093419236.0264712
TCTGCGT356.901373E-736.01392711
CAGCGTC356.901373E-736.0139277
CAGGTAC200.002097002736.01392715
ATGGGGG404.8119546E-836.0139278
AGACTAT404.8119546E-836.0139278
CAGCGCA453.3669494E-936.0139277
CCCCTTG251.439807E-436.0139277
GGGTGAG200.002097002736.0139279
AATCCGC200.002097002736.0139278
Produced by FastQC (version 0.11.5)