FastQCFastQC Report
Thu 26 May 2016
SRR2099122_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099122_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333370
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTGCATGTACCCTTTGAACTATACCAGTTCTCAGATCCCTA39721.1914689384167743No Hit
ACGTGAATCGCTCATCGCCTGCCGCGCCCTCGCCGTGCCGACC13460.4037555868854426No Hit
ACCTGCACCACCCGCGGGATTGAGCTCCACCAGATCCATCGAG12360.3707592164861865No Hit
ACGTGCATGGCAGCGACGATCACTTTACGTGCATTTTTTTTTT12010.3602603713591505No Hit
ACGTGCATACTTTGTCCTGTCACGTGCATTTTTTTTTTTTTTT11490.34466208717041125No Hit
ACGTGCATCGTCACGTGCATTTTTTTTTTTTTTTTTTTTTTTT10270.3080661127276No Hit
ACGAGCATACATGCCGACGATCCAGCAACGTGCATTTTTTTTT9710.29126796052434234No Hit
ACCTGCACCACCCGGCGGGATTGAGCTCCACCAGATCCATCGA8760.26277109517953023No Hit
ACGTGCATAGCCAGTGGAGACACCAGCATCCAGACTCTGGGGC7950.23847376788553257No Hit
ACGTGCATGATGGGGCCCAGAGGGCTCCACACCAGCCCCAGGT7670.23007469178390377No Hit
ACGTGCATGGCAGGCGACGATCACTTTACGTGCATTTTTTTTT7030.21087680355160932No Hit
ACGTGCATACATGCCGACGATCCAGCAACGTGCATTTTTTTTT6730.2018777934427213No Hit
ACCCGCATGAGTACTTCAGGTCTGAACGGCTGTACATAGCCCC6570.19707832138464768No Hit
ACGTGCATATGACCGCTGATGCATGCCGGCGGCATGGAGCCGG6500.19497855235924047No Hit
ACGTGCATCAGTTCCCCACCGCGACGCGGCGCGTGCGCGGGCG6110.18327983921768604No Hit
ACGAGCAGCCTCATGGCACGATGAAGTGGGGTCGGCCTGGATC5500.1649818519962804No Hit
ACGTGCAGGGACTGGCCGTCGCCGGCAATCGATCCGGCCTAAC5420.1625821159672436No Hit
ACGTGCATTTTGTGCTGGAAGATGGAAAGATCATGGCTGACTA5280.1583825779164292No Hit
ACGTGCACTTGTCATGCCTCACGACCTGATCAACTCCAAGCCT5050.15148333683294837No Hit
ACGAGCAGCCTCATTGGCACGATGAAGTGGGGTCGGCCTGGAT5020.15058343582205957No Hit
ACGTGCATGAATTGGTTCCCGCTCTTGCCGCGTCTGCTCCAAC4800.14398416174220835No Hit
ACGTCCATCTATTCCGACACGGATGCTACGGATGAGGATGTAG4740.14218435972043075No Hit
ACCCGCATGAGTAGCTTCAGGTCTGAACGGCTGTACATAGCCC4590.13768485466598676No Hit
ACGTGCATAATTACAGTCCGACGATCGATGTACGTTTCATGAT4470.13408525062243154No Hit
ACGTGTTTAGTAGGAGAAAATCGCCTCGATCAGCAGCTGACCG4360.1307856135825059No Hit
ACGTGCATCCCACGGAGAAACCCCCCTGGCGGCAAGTCCAGCC4350.13048564657887632No Hit
ACGTGCATATGCCCAGTCCGACGATCTCATCACGTGCATAGAT4150.12448630650628431No Hit
ACGAGCTTCAACTTAACAATACAGGACTCTTTCGAGGCCCTGT4130.1238863724990251No Hit
ACGAGCTTCAACTTCCAAGGAAGGCAGCAGGCGCGCAAATTAC4060.12178660347361789No Hit
ACGTGCATTTAGCGTCCGACGATCAGTCAGCTAATTAAGCTTG3970.11908690044095148No Hit
ACGTGCATGCAGAGTTTGTATTTATGGGTCACATGAGATATTT3890.11668716441191468No Hit
ACGTGCATGTCTGCGCCGCCGCAACCAGCTGGTCGTGCTGGCC3780.11338752737198908No Hit
ACGTGCATGATGGGGGCCCAGAGGGCTCCACACCAGCCCCAGG3650.10948795632480428No Hit
ACGTGCATGCGAGCAGTCCGACGATCTACGCACGTCTGGAGAT3580.10738818729939706No Hit
ATTTGCATGTACCCGGCCGACGATCGTAGCAGTTGACATGCAA3520.10558838527761946No Hit
ACGTGCATCTCAACAGTCCGACGATCCACGTACGTGATTAGAT3440.10318864924858265No Hit
ATGCGCATGCATGCGCATTCTGAAGAACGTCTTCGTCCCCCAG3440.10318864924858265No Hit
ACGTGCATAGAAAGCTAGTCCCAGACCCCTCCCCCCAGCTCCC3430.10288868224495305No Hit
ACGTGCATTGATCGTCCGACGATCTACGCACGTGGAGAGATCG3430.10288868224495305No Hit
ACGTGCATTCGACCAGTCCGACGATCTCTCGACGGACTGTAGA3360.10078891321954585No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCCTT200.0018175537.0928437
TCCGCTA200.001829671737.0426758
TTTCCGC308.228919E-637.0426758
TAACACG750.037.0426758
TTAGTTG308.228919E-637.0426758
TTCGGCG403.7807695E-837.0426758
TTTTGCG200.001829671737.0426758
TATGTCG251.2234144E-437.0426758
TCGGCCC551.2732926E-1137.0371139
CGCTCAA650.037.0371139
GTACCGT200.001831022437.037119
TTAGCGT200.001831022437.037119
GAGTAGG251.224497E-437.037119
CTATTAG200.001831022437.037119
TATCGGT200.001831022437.037119
CTTACGG200.001831022437.037119
AACACGA200.001831022437.037119
ATAGGGT551.2732926E-1137.0315487
ATGGCGA501.7644197E-1037.0315487
ATAGCGC200.00183237437.0315487
Produced by FastQC (version 0.11.5)