FastQCFastQC Report
Thu 26 May 2016
SRR2099120_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099120_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157481
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATTCAACCCTAGGTTATGCTGTGGTAACAAACAGCTAC15380.9766257516779802No Hit
GCTATAGCCAGTACATTGGTGTCCTTTATCCAGATCAGTATGG10250.6508721687060661No Hit
GATCTAATGCATTGAGTTCAGTTGTAAGTATAAAGTATTATAT6630.42100316863621645No Hit
GATCTAGCACTTCGGGCAAAACACAGTTCTATGAGAGGCCCTG6510.41338320178307225No Hit
GATCTAGCGTTTTAAGCAATCCACCCGCCTCGGCCTCCCAAAG6470.4108432128320242No Hit
GACCTACCTCCGTTGGTAACGGTGATCTCACCAGTGTTCTCAT5860.37210838132854124No Hit
GATCTAGCAAACTGTTTGAAAAAAGCTGTATTAGTCCATTCTC4880.3098786520278637No Hit
GATCTAGCGCGGCCCACGATGGTCGACGACGCGGCGACGACCC4770.3028936824124815No Hit
GCTCTAGCCTGGGCTGGGAACTCGTGCAGGTGGACGGCGAGCG4730.3003536934614334No Hit
GATCTAGCGTTCTACAGTCCGACGATCCTTATGATCGTCGGAC4580.2908287348950032No Hit
GATCTAGCACCGAAGTCCGACGATCACGTCGATCTGTAGAACT4480.284478762517383No Hit
GATCTAGCTTAACTTGGTATTGCGAAGATCATATGCTCCAGTA4170.26479384814676055No Hit
GATCTAGCAAGTTAGTCCGACGATCCGCTGGATCTAGCTTTTT4160.26415885090899854No Hit
GATCTAGCCCATTGCACCCTGGCGATTTGCGTGCCGCGCTTGA4160.26415885090899854No Hit
GATCTAGCGGGTTCAGAGCAAAGACAACAGGTGCAAAAGAATC4090.2597138702446644No Hit
GATCTTGTTCAGTGACTTCCAAGCCTGGCCTCTGACTGGCTGG4090.2597138702446644No Hit
GATCTAGCGTATACAGTCCGACGATCAGGCCGATCGTCGGACT4090.2597138702446644No Hit
GATCCAGTGTACCGTCCGACGATCGTACTTTAGTTCCGTCAAC4040.25653888405585434No Hit
GATCTAGCATGCTACAGTCCGACGATCCTTATGATCGTCGGAC3880.24637892825166208No Hit
GATCTAGCTGGTAGTGGGCTCCATGTTACACCTAGAACATGGG3830.24320394206285204No Hit
GATCTTCCCTGTAAAAGAAAGAAAGAAAGGGAGAGAAAGGAAC3810.241933947587328No Hit
GATCTAGCTGTTCCTGAAAATCACGGAAAATGAGAAATACACA3780.24002895587404194No Hit
GATCTAGCATCTACTTGCATAATGGGCCATTTGTTTGTCATTG3680.23367898349642177No Hit
GATCTAGCAGGTTCCTTATGCAATCATGAGGCATAAGGTTCAA3640.2311389945453737No Hit
GATCTAGCTAAGCTACAGTCCGACGATCTTTCGGACTGTAGAA3330.21145408017475123No Hit
GAACAAGCGGCGTGAAACCCGCCGTGATGACGCCCCAGGTGAT3310.2101840856992272No Hit
GATCTAGCACGCCCGACGATCGAGAAGATCTAGCTTTTTTCAG3290.20891409122370316No Hit
GATCTAGCACGCCAAGATCTAGCTTTTTTCAGGAGTTTTCATT3210.20383411332160706No Hit
GATCTAGCTTAACGTTGGTATTGCGAAGATCATATGCTCCAGT2880.18287920447546052No Hit
GATCTAGCGTTTGCAGTTGCTGAGGTCTGGAACTCTCAAGGCT2880.18287920447546052No Hit
GATCTAGCCCATGTGAGAGATCAGCATCAATACTGTCAAGTTT2820.17906922104888842No Hit
GATCTAGCCTCGATCTACAGTCCGACGATCTTGCAGATCTCTG2770.17589423486007835No Hit
GATCTAGCAGATTTCTACAGTCCGACGATCTTCTGGATCGTCG2740.17398924314679232No Hit
GATCTAGAGCTTGTACAGTCCGACGATCAAAATGATCTAGATC2730.17335424590903029No Hit
GTTCTGGCTCGACGCGCCACTCTTGACCTGCGCCAGCGCGGAC2650.16827426800693415No Hit
GCTATAGCCAGTAGCATTGGTGTCCTTTATCCAGATCAGTATG2530.16065430115378998No Hit
GATCTAGCCTAAGGATTACAGTCTATCATGAAGAGAAGCCAGG2510.15938430667826595No Hit
GATCCACCTATCAGTGGAATATGGCAAGAAAACTGAAAATCAT2400.15239933706288378No Hit
GATCTATTCAACCCCTAGGTTATGCTGTGGTAACAAACAGCTA2360.1498593481118357No Hit
GATCTAGCGCCCAGTCCGACGATCCTGGCGATCTGTAGAACTC2260.14350937573421554No Hit
GATCTAGCAGGTATCTACAGTCCGACGATCCCGTTGATCTGTA2260.14350937573421554No Hit
GATCTAGCGTGAGATGATCAAATTCCTGCTGGGCACTGGTGGC2220.14096938678316748No Hit
GATCTAGCAGTGAAGTCCGACGATCTGTTGGATCTCACTGATC2210.14033438954540547No Hit
GATCTAGCGATCAGACTGTTTTCCACAGCCCTGTGATTGCATC2180.1384293978321194No Hit
GATCTAGCGATCTTCTACAGTCCGACGATCCGTTCGATCTGTA2180.1384293978321194No Hit
GATCTAGCTTAACTTTGGTATTGCGAAGATCATATGCTCCAGT2130.13525441164330934No Hit
GATCTAGCCCAAACAGTCCGACGATCCTATTGATCTAGCTGAT2100.1333494199300233No Hit
GATCTCGAAACTCCAGTCCGACGATCACCGAGATCGTCGGACT2090.13271442269226127No Hit
GATCTAGCTCTACAGAGAATTGTAGCCAGGATTTTGAGATTTA2020.12826944202792717No Hit
GTTCTGGCTCGACGGCGCCACTCTTGACCTGCGCCAGCGCGGA2010.12763444479016514No Hit
GATCTAGCGAGTAGTGGCCCTCGAAGGCCACGTGATAGTCGAT2000.12699944755240314No Hit
GATCTAGCCTACTGTCCGACGATCCACTAGATCTAAGCGATCG1990.1263644503146411No Hit
GATCTAGCGACTATACAGTCCGACGATCAGCGGGATCTAGAAC1990.1263644503146411No Hit
GATCTAGCTTATAGTCCGACGATCGCTGGATCGTCGGACTGTA1960.12445945860135507No Hit
GACATAGCTCGACTATCAACAGTGACTACGTTGATAGTCTCGA1920.121919469650307No Hit
GATCTAGCCCTACAGTCTCTGCTTTGCAGAGACTTGAAATGCC1910.121284472412545No Hit
GGTCTGGCAGTCTCAGTCAAGCAGAGCAGCTTGCACTGATTTT1900.12064947517478299No Hit
GATCTCGTCGACTGGAATATGGCGAGAAAACTGAAAATCATGG1900.12064947517478299No Hit
GATCTAGCACTATATGGGGTTTCACCATGTTGGCCAGGGTGGT1900.12064947517478299No Hit
GATCTAGCTAGTCGGCAGGTGGATTTCTGAGTTCGAGGCCAGC1890.12001447793702097No Hit
GATCTAGCCGTGTCTACAGTCCGACGATCTATTCGATCGTCGG1850.1174744889859729No Hit
GATCTAGCTTGCAGAAAAAGTTGGAAAATTTAGAAATGTCCAC1840.11683949174821089No Hit
GATCTAGCCAGTTACAGTCCGACGATCGGTGTGATCGTCGGAC1790.1136645055594008No Hit
AAACTAGCGTAAAGCGACGATCGTACAAAACTGTAGTCAACTG1780.11302950832163879No Hit
GATCTAGCTCGGACTACAGTCCGACGATCGTGCTGTAGAACTC1770.11239451108387677No Hit
GAGCTAGAAACATCCGACGATCCGAAAGATCTAGCTCATGTTG1760.11175951384611478No Hit
GATCTAGCTCCGCGTCCGACGATCCGTTGGACTGTAGAACTCT1740.11048951937059075No Hit
GATCTAGCTATTGGTTCAGGGTCAACTGCACATCAGTCAAAAA1720.10921952489506671No Hit
GATCTAGCTGGTATGTGGGCTCCATGTTACACCTAGAACATGG1670.10604453870625664No Hit
AGTCTAGCCTGTTATTCAAGAAGGAAACTGTGGGAGAATTCGA1600.10159955804192253No Hit
AAACTAGCGTAAACGACGATCGTACAAAACTGTAGTCAACTGC1590.10096456080416051No Hit
GATCTAGCAGGTTCTTGAACCTTATGCAATCATGAGGCATAAG1580.1003295635663985No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCGG200.001815130537.09076737
GACTCCT251.2111867E-437.09076737
CTTGTAT251.2248807E-437.0200278
GTTGAGA200.001832236237.02002713
CTTAACT800.037.0200278
TAGAGTT200.001832236237.02002712
AGGTATC308.2358E-637.0200279
CAGGTAT403.7793143E-837.0200278
CGGCGTC200.001832236237.0200278
TTAGGAT200.001832236237.0200279
TAGAGAT251.2248807E-437.02002712
GTACCGT452.575689E-937.0200279
CAAACTA251.2248807E-437.0200278
GTACCGG308.2358E-637.0200279
ACGCCTC251.2248807E-437.0200279
ATGATTG251.2248807E-437.02002711
TCCGCGT308.2358E-637.0200279
CATATCT200.001832236237.0200278
AGTCATC200.001832236237.0200279
CTCCGTG308.2358E-637.0200278
Produced by FastQC (version 0.11.5)