FastQCFastQC Report
Thu 26 May 2016
SRR2099119_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099119_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101919
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATTCAACCCTAGGTTATGCTGTGGTAACAAACAGCTAC9900.9713596091013452No Hit
GCTATAGCCAGTACATTGGTGTCCTTTATCCAGATCAGTATGG6600.64757307273423No Hit
GATCTAGCGTTTTAAGCAATCCACCCGCCTCGGCCTCCCAAAG4200.41209195537632826No Hit
GATCTAGCACTTCGGGCAAAACACAGTTCTATGAGAGGCCCTG4180.4101296127316791No Hit
GATCTAATGCATTGAGTTCAGTTGTAAGTATAAAGTATTATAT4010.39344970025216097No Hit
GACCTACCTCCGTTGGTAACGGTGATCTCACCAGTGTTCTCAT3890.38167564438426593No Hit
GATCTAGCTGTTCCTGAAAATCACGGAAAATGAGAAATACACA3380.3316359069457118No Hit
GCTCTAGCCTGGGCTGGGAACTCGTGCAGGTGGACGGCGAGCG3130.307106623887597No Hit
GATCTAGCGCGGCCCACGATGGTCGACGACGCGGCGACGACCC3120.3061254525652724No Hit
GATCTAGCAAACTGTTTGAAAAAAGCTGTATTAGTCCATTCTC2920.2865020261187806No Hit
GATCTAGCTTAACTTGGTATTGCGAAGATCATATGCTCCAGTA2880.2825773408294822No Hit
GATCTAGCAAGTTAGTCCGACGATCCGCTGGATCTAGCTTTTT2830.2776714842178593No Hit
GATCTAGCGTTCTACAGTCCGACGATCCTTATGATCGTCGGAC2810.2757091415732101No Hit
GATCTAGCACCGAAGTCCGACGATCACGTCGATCTGTAGAACT2810.2757091415732101No Hit
GATCTAGCGGGTTCAGAGCAAAGACAACAGGTGCAAAAGAATC2720.2668785996722888No Hit
GATCCAGTGTACCGTCCGACGATCGTACTTTAGTTCCGTCAAC2650.26001040041601664No Hit
GATCTAGCCCATTGCACCCTGGCGATTTGCGTGCCGCGCTTGA2600.2551045438043937No Hit
GATCTAGCGTATACAGTCCGACGATCAGGCCGATCGTCGGACT2600.2551045438043937No Hit
GATCTAGAGCTTGTACAGTCCGACGATCAAAATGATCTAGATC2530.24823634454812152No Hit
GATCTAGCTGGTAGTGGGCTCCATGTTACACCTAGAACATGGG2490.2443116592588232No Hit
GATCTTGTTCAGTGACTTCCAAGCCTGGCCTCTGACTGGCTGG2490.2443116592588232No Hit
GATCTAGCATGCTACAGTCCGACGATCCTTATGATCGTCGGAC2380.23351877471325266No Hit
GATCTTCCCTGTAAAAGAAAGAAAGAAAGGGAGAGAAAGGAAC2360.2315564320686035No Hit
GATCTAGCATCTACTTGCATAATGGGCCATTTGTTTGTCATTG2330.22861291810162976No Hit
GAACAAGCGGCGTGAAACCCGCCGTGATGACGCCCCAGGTGAT2300.22566940413465594No Hit
GATCTAGCAGGTTCCTTATGCAATCATGAGGCATAAGGTTCAA2260.2217447188453576No Hit
GATCTAGCACGCCCGACGATCGAGAAGATCTAGCTTTTTTCAG2080.20408363504351495No Hit
GATCTAGCACGCCAAGATCTAGCTTTTTTCAGGAGTTTTCATT1970.19329075049794445No Hit
GATCTAGCTAAGCTACAGTCCGACGATCTTTCGGACTGTAGAA1960.19230957917561986No Hit
GTTCTGGCTCGACGCGCCACTCTTGACCTGCGCCAGCGCGGAC1900.1864225512416723No Hit
GATCTAGCTTAACGTTGGTATTGCGAAGATCATATGCTCCAGT1870.18347903727469855No Hit
GATCTAGCGTTTGCAGTTGCTGAGGTCTGGAACTCTCAAGGCT1860.18249786595237397No Hit
GATCTAGCCCATGTGAGAGATCAGCATCAATACTGTCAAGTTT1820.17857318066307556No Hit
GATCTAGCCTCGATCTACAGTCCGACGATCTTGCAGATCTCTG1810.17759200934075098No Hit
GATCTAGCCTAAGGATTACAGTCTATCATGAAGAGAAGCCAGG1590.15600624024961No Hit
GATCTAGCAGATTTCTACAGTCCGACGATCTTCTGGATCGTCG1590.15600624024961No Hit
GATCTAGCGTGAGATGATCAAATTCCTGCTGGGCACTGGTGGC1580.15502506892728538No Hit
GATCCACCTATCAGTGGAATATGGCAAGAAAACTGAAAATCAT1570.1540438976049608No Hit
GCTATAGCCAGTAGCATTGGTGTCCTTTATCCAGATCAGTATG1560.1530627262826362No Hit
GATCTATTCAACCCCTAGGTTATGCTGTGGTAACAAACAGCTA1530.15011921231566244No Hit
GATCTAGCAGGTATCTACAGTCCGACGATCCCGTTGATCTGTA1480.14521335570403948No Hit
GATCTAGCTTAACTTTGGTATTGCGAAGATCATATGCTCCAGT1470.1442321843817149No Hit
GATCTAGCGCCCAGTCCGACGATCCTGGCGATCTGTAGAACTC1460.1432510130593903No Hit
GATCTAGCGATCAGACTGTTTTCCACAGCCCTGTGATTGCATC1430.14030749909241652No Hit
GATCTAGCAGTGAAGTCCGACGATCTGTTGGATCTCACTGATC1420.13932632777009193No Hit
GATCTAGCGATCTTCTACAGTCCGACGATCCGTTCGATCTGTA1360.1334392998361444No Hit
GATCTCGAAACTCCAGTCCGACGATCACCGAGATCGTCGGACT1330.1304957858691706No Hit
GATCTAGCACTATATGGGGTTTCACCATGTTGGCCAGGGTGGT1290.12657110057987225No Hit
GATCTAGCGAGTAGTGGCCCTCGAAGGCCACGTGATAGTCGAT1290.12657110057987225No Hit
GGTCTGGCAGTCTCAGTCAAGCAGAGCAGCTTGCACTGATTTT1280.12558992925754767No Hit
GATCTAGCTAGTCGGCAGGTGGATTTCTGAGTTCGAGGCCAGC1280.12558992925754767No Hit
GATCTCGTCGACTGGAATATGGCGAGAAAACTGAAAATCATGG1250.12264641529057389No Hit
GTTCTGGCTCGACGGCGCCACTCTTGACCTGCGCCAGCGCGGA1250.12264641529057389No Hit
GATCTAGCTCTACAGAGAATTGTAGCCAGGATTTTGAGATTTA1240.1216652439682493No Hit
GATCTAGCTTGCAGAAAAAGTTGGAAAATTTAGAAATGTCCAC1240.1216652439682493No Hit
GATCTAGCGACTATACAGTCCGACGATCAGCGGGATCTAGAAC1220.1197029013236001No Hit
GATCTAGCCAGTTACAGTCCGACGATCGGTGTGATCGTCGGAC1220.1197029013236001No Hit
GATCTAGCCCTACAGTCTCTGCTTTGCAGAGACTTGAAATGCC1200.11774055867895095No Hit
GATCTAGCCGTGTCTACAGTCCGACGATCTATTCGATCGTCGG1180.11577821603430175No Hit
GATCTAGCTGGTATGTGGGCTCCATGTTACACCTAGAACATGG1160.11381587338965256No Hit
GATCTAGCATTAGAAGAAAACGTCCCAAACCTAAAGAAAGAGA1140.11185353074500338No Hit
GATCTAGCTCCGCGTCCGACGATCCGTTGGACTGTAGAACTCT1120.1098911881003542No Hit
AGTCTAGCCTGTTATTCAAGAAGGAAACTGTGGGAGAATTCGA1120.1098911881003542No Hit
AAACTAGCGTAAAGCGACGATCGTACAAAACTGTAGTCAACTG1110.10891001677802961No Hit
GATCTAGCTATTGGTTCAGGGTCAACTGCACATCAGTCAAAAA1100.10792884545570502No Hit
GATCTAGCTTATAGTCCGACGATCGCTGGATCGTCGGACTGTA1100.10792884545570502No Hit
GATCTAGCTCGGACTACAGTCCGACGATCGTGCTGTAGAACTC1090.10694767413338042No Hit
GCTCTCGCCAGTGGTCCGACGATCCAGTCGGACTGTAGAACTC1080.10596650281105584No Hit
GATCTAGCTAATATAAAAATGCTGAAGAAACCCAAGTTTGAAT1080.10596650281105584No Hit
GAGCTAGAAACATCCGACGATCCGAAAGATCTAGCTCATGTTG1060.10400416016640666No Hit
GATCTAGCAGGTTCTTGAACCTTATGCAATCATGAGGCATAAG1050.10302298884408206No Hit
GATCTAGCCCTATGAGTTCTACAGTCCTCAATCGCTAGATCAT1040.10204181752175748No Hit
GATCTAGCACTAAGAGAAAACTGAAAATCACGGAAAATGAGAA1040.10204181752175748No Hit
GATCTAGCTTTTGCTACAGTCCGACGATCTATTCGATCGTCGG1030.10106064619943289No Hit
AAACTAGCGTAAACGACGATCGTACAAAACTGTAGTCAACTGC1030.10106064619943289No Hit
GATCTAGCCTACTGTCCGACGATCCACTAGATCTAAGCGATCG1020.10007947487710829No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTG251.2231614E-437.0132610
TAATATA251.2231614E-437.013269
GGGTGAC251.2231614E-437.0132612
CAAACTA251.2231614E-437.013268
TCCGCCC251.2231614E-437.013268
CGTATAC251.2231614E-437.013268
TCACAGT251.2231614E-437.0132612
TAGAAGA251.2231614E-437.0132611
TAACGTT251.2231614E-437.0132610
CACCTGT251.2231614E-437.013268
TGTTCCT251.2231614E-437.013269
CTTGATT251.2231614E-437.013269
TTAACGT251.2231614E-437.013269
TCCGTAT251.2231614E-437.013269
AGTTAGT251.2231614E-437.0132610
GGTGACG251.2231614E-437.0132613
CCAACTC251.2231614E-437.013268
CGCGTAT251.2231614E-437.013268
CGCGTAG251.2231614E-437.013268
AAAATAG251.2231614E-437.0132614
Produced by FastQC (version 0.11.5)