FastQCFastQC Report
Thu 26 May 2016
SRR2099118_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099118_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188848
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACACACCCGCGAGTGAGGTGTGGGATGGGCAGGGTCCCTC18640.9870371939337456No Hit
TGCATGTAGCCATGTGAGGTTGTGGATGCCCTCTTCCTGGAAG13800.7307464204015928No Hit
TGCATGTAGCCATGGTGAGGTTGTGGATGCCCTCTTCCTGGAA10920.57824281962213No Hit
TGCACGGAGCCCTGTCGAGGGTGAGATAGGCGACCACGCCGCC10590.5607684486994832No Hit
TGCACGCTTTTCCCAGACCCGAGGCGCCGATGTGGTTCGTGCC9660.511522494281115No Hit
TGCACGTACTTATGTGGCTCAGTGGTTGAGCGTCTGCCTTTGG9560.5062272303651614No Hit
TGCACGTACTTTCCTATACAGCCTTCCTGGAGTGGCAGTAATG9020.4776328052190121No Hit
CGCTCGTACAGTCTCCGACGATCCAGTTCATATTGATCGAACG8060.4267982716258579No Hit
TGCACGTACTTATGGTGGCTCAGTGGTTGAGCGTCTGCCTTTG7030.372257053291536No Hit
TGCATGTGACGATGGTGCATGACATGACAAGGTAGATGTAGTT6590.3489578920613403No Hit
TGCACGTACACAGAGGGAACCGTACAGCAGCACCTTTACCTGG6350.3362492586630518No Hit
TGCACGTAAAGGCCAGTCCGACGATCGTATATGCACGGGAGAT6150.3256587308311446No Hit
TGCACGGAGCCCTGGTCGAGGGTGAGATAGGCGACCACGCCGC5840.3092434126916886No Hit
TGGAGGTACATGCCCGGAAGGCGTCTGTGGTCTTTGAACCACA5700.3018300432093536No Hit
TGCACGTACCGGTAAAAGCGACAAACCATCAGTCCAATAGGCC5440.28806235702787425No Hit
TGCACCTTACCCGCGTGGTTCGCCGTACGCAGATCGAAGCGAT5070.2684698805388461No Hit
TCCACGTGCTATGAGCAAACTTCAGTGTACTCAACAACCCAAC4370.23140303312717106No Hit
AGGACGTATCGAACAAAAACAGTGACTACGTTTTTTTTTTCGA4130.21869439972888247No Hit
TGCACGTATCAGAAAGCGACCATATCTCTAGATTTTGCCCATC4070.21551724137931033No Hit
TGCACGTAGTCTCACCAAAAATAATGAACTTAAAACAAAAATA4020.21286960942133357No Hit
TTCACGTAGCTTTTGCACTATCATTTTGTATAGATGCAGTTAG4000.21181055663814286No Hit
TGCACGTACTCCCGTCCGACGATCTGTAGAACTCTGAACCCCT3890.20598576633059393No Hit
GGCATGTACTCATAGCTGCTTAACACCTATCTGCCACATATAC3770.19963144963144963No Hit
TGCACGTATTCGACGGCACGGGCTGCTCCACGGGCGAGAACCA3760.1991019232398543No Hit
TGCACGTAACCTTCGTCCGACGATCCTAATTGCACGTATTTTT3730.1975133440650682No Hit
TGCACGTGATGTCCAACCGCAGAGCGGTTTCTGCCATCACGGA3570.1890409217995425No Hit
TGCACGTACCGCGTTCCAGCCCTTGCGCAACCTGCCCCATGTC3530.18692281623316104No Hit
TGCACGTAGACGCTCGGCCGTGCACGTATTTTTTTTTTTTTTT3500.18533423705837498No Hit
TGCTCATATTTACTTCTGCCGCAGCCAGCGAAAAGCTCTCAAG3500.18533423705837498No Hit
TGCACGTAATAAAGGCTCGGGATTGTCTTTGTTGCAAGGTCTC3350.17739134118444463No Hit
TGGAGGTACATGCTCCGGAAGGCGTCTGTGGTCTTTGAACCAC3330.17633228840125392No Hit
TGCACGTACGTCTACAGTCCGACGATCAACGCTGCACGTATGA3210.16997797170210963No Hit
TCCAAGTATCCCAGTCTACGGCCATACCACCCTGAACGCGCCC3150.1668008133525375No Hit
TGCACGTATATAACAGTCCGACGATCCCCGGTGCACGTAATTA3110.16468270778615604No Hit
TGCACGTATGGGTGGAAAAGTGAGAGGTCAGACTCCCAAGGTG3100.1641531813945607No Hit
TGCACGTATAACAAAAGCTAAAAGGGAACTAGCTGAATCCCGA2940.15568075912903498No Hit
TGCTCATATTTACCTTCTGCCGCAGCCAGCGAAAAGCTCTCAA2910.15409217995424893No Hit
GGCACGTCGCGGCCATCGAATTGACAGCCCAGCCGGCACCTCC2850.1509150216046768No Hit
TGCACGTACCAGGGCAGGAAGTTTGGAAGTGACGATCAGCCGG2770.14667881047191392No Hit
TGCACGTAGCATTCAGTCCGACGATCTCTTATGCACGTACGAT2630.13926544098957894No Hit
TGCACGTATACGAGAAAAAGAATGCCAAAATCAGCAGCCTCCT2600.13767686181479286No Hit
TGGAGGTACATGCCAGACGCCGGAAGGCGTCTGTGGTCTTTGA2590.1371473354231975No Hit
TGCACGTAAGTAGGAGTTCTCCAGTCCGACGATCTATTATGCA2550.13502922985681606Illumina Small RNA Adapter 1 (95% over 21bp)
TGCACGTATAATTGACAATTGTAGCCACTGAAAAGTGAAAGAA2530.13397017707362535No Hit
TGCACCAAAGGGCGTGTTTTAGTTTTCAAGTGCTGTTATGTTT2470.1307930187240532No Hit
TGCATGTTTCCGATTAGGATGCTGAGCTCACCATCTAGTAGGC2430.1286749131576718No Hit
TGCACGTACCTTAGGGCTTGCACGTATTTTTTTTTTTTTTTTT2340.12390917563331355No Hit
TGCACGTATTCGACCCGGCACGGGCTGCTCCACGGGCGAGAAC2290.12126154367533679No Hit
TGCACGTATCACTCAGTCCGACGATCCACGTGCACAAAGGATC2270.12020249089214606No Hit
TGCACGTATCAGGCAGTCCGACGATCCATTCTGCACGAGTGAT2230.11808438532576464No Hit
TGCACGTAGATCGACAGTCCGACGATCCTGCATGCTGGATCGT2170.11490722697619249No Hit
TGCACGTATTCGACCGGCACGGGCTGCTCCACGGGCGAGAACC2100.111200542235025No Hit
TGCACGTAAGCCCACAGTCCGACGATCGTGCATGCTAGATCGT2090.11067101584342964No Hit
TGCACGTATTGCTGAAAACTAAAAAGGCGTTATCATCGAGTCT2020.10696433110226214No Hit
TGCATGTAGCCATAGTGAGGTTGTGGATGCCCTCTTCCTGGAA1970.10431669914428535No Hit
TGCACGTATCACTCAGTCCGACGATCTCGCGTGCAAATCTGAT1950.10325764636109463No Hit
TGCACGCTTTTCCGCAGACCCGAGGCGCCGATGTGGTTCGTGC1930.10219859357790394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGCC1300.037.08844837
CACCCAT200.001816606437.08844837
CGGAAGT650.037.039265
CGTATCC1750.037.039265
TACCATT650.037.039267
ACACCCG4250.037.039266
GTCTCAC650.037.039269
ATTCGAG650.037.039268
GTAGCGG650.037.039266
CCCGTAC650.037.039263
GTATATC1300.037.039266
CGTAAGG1750.037.039265
CGTAACA1750.037.039265
ACGTAAC8150.037.039264
ATTGCTA650.037.039268
ATTTACC551.2732926E-1137.0392578
AAGTATC900.037.0392574
CAGGTAG850.037.0392573
AGCCGGA200.001828489637.0392578
ACCGGTT200.001828489637.0392578
Produced by FastQC (version 0.11.5)