FastQCFastQC Report
Thu 26 May 2016
SRR2099117_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099117_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129673
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACACACCCGCGAGTGAGGTGTGGGATGGGCAGGGTCCCTC12280.9469974474254471No Hit
TGCATGTAGCCATGTGAGGTTGTGGATGCCCTCTTCCTGGAAG8840.6817147748567551No Hit
TGCATGTAGCCATGGTGAGGTTGTGGATGCCCTCTTCCTGGAA7200.5552428030507507No Hit
TGCACGGAGCCCTGTCGAGGGTGAGATAGGCGACCACGCCGCC7130.5498446091322018No Hit
TGCACGCTTTTCCCAGACCCGAGGCGCCGATGTGGTTCGTGCC6790.5236248100992497No Hit
TGCACGTACTTATGTGGCTCAGTGGTTGAGCGTCTGCCTTTGG6180.47658340595189436No Hit
TGCACGTACTTTCCTATACAGCCTTCCTGGAGTGGCAGTAATG5880.45344828915811314No Hit
CGCTCGTACAGTCTCCGACGATCCAGTTCATATTGATCGAACG5130.3956104971736599No Hit
TGCACGTACTTATGGTGGCTCAGTGGTTGAGCGTCTGCCTTTG4540.35011143414589No Hit
TGCATGTGACGATGGTGCATGACATGACAAGGTAGATGTAGTT4450.34317089910775567No Hit
TGCACGTACACAGAGGGAACCGTACAGCAGCACCTTTACCTGG4200.3238916351129379No Hit
TGCACGTAAAGGCCAGTCCGACGATCGTATATGCACGGGAGAT3780.29150247160164416No Hit
TGGAGGTACATGCCCGGAAGGCGTCTGTGGTCTTTGAACCACA3650.2814772543243389No Hit
TGCACGGAGCCCTGGTCGAGGGTGAGATAGGCGACCACGCCGC3560.27453671928620454No Hit
TGCACGTACCGGTAAAAGCGACAAACCATCAGTCCAATAGGCC3540.27299437816661914No Hit
TGCACCTTACCCGCGTGGTTCGCCGTACGCAGATCGAAGCGAT3270.25217277305221597No Hit
TTCACGTAGCTTTTGCACTATCATTTTGTATAGATGCAGTTAG2690.2074448805842388No Hit
TCCACGTGCTATGAGCAAACTTCAGTGTACTCAACAACCCAAC2670.20590253946465342No Hit
TGCACGTATCAGAAAGCGACCATATCTCTAGATTTTGCCCATC2660.20513136890486067No Hit
TGCACGTAGTCTCACCAAAAATAATGAACTTAAAACAAAAATA2600.20050434554610444No Hit
TGCACGTATTCGACGGCACGGGCTGCTCCACGGGCGAGAACCA2540.19587732218734819No Hit
TGCACGTACCGCGTTCCAGCCCTTGCGCAACCTGCCCCATGTC2450.18893678714921378No Hit
GGCATGTACTCATAGCTGCTTAACACCTATCTGCCACATATAC2440.1881656165894211No Hit
TGCACGTAACCTTCGTCCGACGATCCTAATTGCACGTATTTTT2440.1881656165894211No Hit
TGCACGTACTCCCGTCCGACGATCTGTAGAACTCTGAACCCCT2400.18508093435025025No Hit
AGGACGTATCGAACAAAAACAGTGACTACGTTTTTTTTTTCGA2390.18430976379045755No Hit
TGCACGTATACGAGAAAAAGAATGCCAAAATCAGCAGCCTCCT2380.18353859323066482No Hit
TGCTCATATTTACTTCTGCCGCAGCCAGCGAAAAGCTCTCAAG2320.17891156987190857No Hit
TGCACGTAGACGCTCGGCCGTGCACGTATTTTTTTTTTTTTTT2300.17736922875232314No Hit
TGCACGTGATGTCCAACCGCAGAGCGGTTTCTGCCATCACGGA2270.175055717072945No Hit
TGGAGGTACATGCTCCGGAAGGCGTCTGTGGTCTTTGAACCAC2260.1742845465131523No Hit
TGCACGTACGTCTACAGTCCGACGATCAACGCTGCACGTATGA2200.16965752315439606No Hit
TGCACGTAATAAAGGCTCGGGATTGTCTTTGTTGCAAGGTCTC2160.16657284091522523No Hit
TGCACGTATGGGTGGAAAAGTGAGAGGTCAGACTCCCAAGGTG2140.1650304997956398No Hit
TGCACGTATAATTGACAATTGTAGCCACTGAAAAGTGAAAGAA2010.1550052825183346No Hit
TGCTCATATTTACCTTCTGCCGCAGCCAGCGAAAAGCTCTCAA1990.15346294139874916No Hit
TGCACGTATATAACAGTCCGACGATCCCCGGTGCACGTAATTA1980.15269177083895644No Hit
TCCAAGTATCCCAGTCTACGGCCATACCACCCTGAACGCGCCC1970.15192060027916376No Hit
TGCACGTATAACAAAAGCTAAAAGGGAACTAGCTGAATCCCGA1950.1503782591595783No Hit
GGCACGTCGCGGCCATCGAATTGACAGCCCAGCCGGCACCTCC1850.14266655356165123No Hit
TGGAGGTACATGCCAGACGCCGGAAGGCGTCTGTGGTCTTTGA1830.1411242124420658No Hit
TGCACGTAAGTAGGAGTTCTCCAGTCCGACGATCTATTATGCA1700.1310989951647606Illumina Small RNA Adapter 1 (95% over 21bp)
TGCACGTAGACAGGGGCAAGCTTCTAGCCTGTATTGCCTCACG1680.12955665404517516No Hit
TGCACGTACCAGGGCAGGAAGTTTGGAAGTGACGATCAGCCGG1660.12801431292558976No Hit
TGCACGTACCTTAGGGCTTGCACGTATTTTTTTTTTTTTTTTT1660.12801431292558976No Hit
TGCATGTTTCCGATTAGGATGCTGAGCTCACCATCTAGTAGGC1640.12647197180600434No Hit
TGCACGTATTCGACCCGGCACGGGCTGCTCCACGGGCGAGAAC1610.12415846012662621No Hit
TGCACGTAGCATTCAGTCCGACGATCTCTTATGCACGTACGAT1600.12338728956683348No Hit
TGCACCAAAGGGCGTGTTTTAGTTTTCAAGTGCTGTTATGTTT1590.1226161190070408No Hit
TGCACGTACATTCAGGAAATGGGGACACCAGATGTGCGCATTG1520.11721792508849183No Hit
TGCACGTAATCCGGGAAAAAGCTGGGTGGGAGATGATGAAATA1490.1149044134091137No Hit
TGCACGTAGATCGACAGTCCGACGATCCTGCATGCTGGATCGT1400.10796387837097932No Hit
TGCACGTATTGCTGAAAACTAAAAAGGCGTTATCATCGAGTCT1360.10487919613180847No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATTT251.225334E-437.01157813
GGTCGAG551.2732926E-1137.01157414
GTTAACG200.001833100337.01157413
CTCGGCC200.001833100337.01157413
AACGAAA200.001833100337.01157416
TCACTAG200.001833100337.01157413
ACCCGGC200.001833100337.01157413
GCGTATT200.001833100337.01157413
AAAGCTA200.001833100337.01157414
TGGTCGA551.2732926E-1137.01157413
AAGCTAA200.001833100337.01157415
CGCCAAG200.001833100337.01157413
TCGTTAC308.259325E-636.99730324
ACCGGTA452.582965E-936.9973038
GTACCAG452.582965E-936.9973036
TACCCCT452.582965E-936.9973037
CGTGCTA600.036.9973035
TAGACTC308.259325E-636.9973037
TAGACGC452.582965E-936.9973037
ACACCCG2550.036.9973036
Produced by FastQC (version 0.11.5)