FastQCFastQC Report
Thu 26 May 2016
SRR2099116_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099116_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390577
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTGCACATGGGACTCACTATAGTGAGTCGTATTACCGGCC27700.7092071473742694No Hit
AGTCTGCAGTCTTACAGTCCGACGATCTGTATAGTCTGCATTT22250.569670001049729No Hit
GATCTGCACATGGAGTGAGTCGTATTACCGGCCGACGATCCAT13400.3430821579355671No Hit
AGTCTGAATCTTAATAAGCACGACGTAAGTCACTTTTAATATT12850.3290004275725401No Hit
AGTATGCCGAGTGCTGGAGGTGGCAACGCCAATCAGCAACGAC11530.2952042747012753No Hit
AGTCTGCACTAATACTTTCGTTTAATTTAAGATTTCTACGCTT10620.2719054117369943No Hit
AGTCTGCATTTGTATCAGGGTGGGATCAGCTCTTACAACTTCT7880.20175279138300514No Hit
AGTCTGCACCGGTGGATACCCAGCTCAGAGAAGAAGAGGAGGA7580.19407184754862677No Hit
AGTCTGCAGTATTAAATCACGGAAAATGAGAAATACACACTTT7120.1822944003359133No Hit
AGTCTGCACGGAGGTGCAGTGGCTCACGCCTATAATCCTTGCT7040.1802461486467457No Hit
AGTCTGGGCTTCAGGCGGCCCGCGCGGCCAAGGAACTGGAAGA6880.1761496452684106No Hit
AGTCTGCAGTGTAAGTTTGGGAATGGATATCTCAGACCCTACA6840.17512551942382681No Hit
AGTCTGCACGCTCCCGACGATCATTTCAGTCTGCATTTTTTTT6820.1746134565015349No Hit
AGTCTGGGCTTCAGCGGCCCGCGCGGCCAAGGAACTGGAAGAC6320.16181188344423764No Hit
AGTGTTCAGTCACGAGCAAGTGTGACATCCATTTATGCAATCT6090.15592315983788088No Hit
AGTCTGGGCCAGGGAATCTACCCATCTCTACGGAATAACGCAT6060.15515506545444305No Hit
AATCTGCACATGGGCAGAAAGAGTGTCTCAAACCTGGTATATA5890.15080253061496196No Hit
AGTCTGCATTACGCCGGCGGTGTGCAGGCAGAGCAGCGGCGTG5890.15080253061496196No Hit
AGTCTGCACTCTGGGAAAAGGGGAAAAGCAAGTGGGAGCCTGT5840.14952237330923224No Hit
AGTCTGCACATCAAGTGACGTCCATGAGCGGTCGAAGCGTTGG5820.14901031038694035No Hit
AGTCTGCACGTGGAGTCACTGCAGGCCAACGTGCAGCGCCTGA5410.1385130204799566No Hit
AATCTGCGCAGTAGTCTACGAGCCCTCAGCGGTGATTCAACTG5340.13672080025193495No Hit
AGTCTGCAGTCGTACTCCATAAGCTCCATACCAATCCCCATCA5080.13006398226214036No Hit
AATCAGCATCAGACATGGATCTGACTTTTTTTGGTTTCTGAGA4870.12468732157807552No Hit
AGTCTGGGCCAGGCATCTCTACGGAATAACGCATGGAAACGTG4500.11521415751567553No Hit
AGTCTGCATCGGTGCCAGCTGGGTTCAGTGGCTCATGCCTGTA4410.11290987436536201No Hit
AGTCTGCAGTATACTACAGTCCGACGATCTTTGGCTAGATCGT4390.11239781144307014No Hit
AGTCTGCATACTACAGTCCGACGATCAATGCAGACTCCATAGA4210.1077892451424431No Hit
AGTCGGCCGGTCGAGCATGCCTCACAGCACACCTTCACAGGCT4110.10522893053098366No Hit
AGTCTGCATCAACCAGAGGTCCCTCCTCTTATTGTAAAGCTAG3940.10087639569150257No Hit
AGTATGCATCATCGGCTCCAGCTGGATCAAAAATGAGGATATT3920.10036433276921068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGCCT308.154899E-637.09387236
TAGTTAT200.001836101837.01781513
CACGAAT900.037.0130737
CAAAGCG452.6011548E-937.0130737
CGGGTGG308.277837E-637.013079
CAAACGC800.037.013077
GCAAGCG1700.037.013076
GGTCGCC308.277837E-637.013079
CACGGGT650.037.013077
CAACGGT403.810419E-837.013077
CACGGCT251.229574E-437.013077
CAGCCGT700.037.013077
ACCGCGT251.229574E-437.013078
CGGTTGT355.6030876E-737.013079
ATTACGG251.229574E-437.013078
GCAAATC650.037.013076
GCACGCG850.037.013076
ATTCGCA403.810419E-837.013078
TTGTACC308.277837E-637.013078
CAATTAT200.001837257837.013077
Produced by FastQC (version 0.11.5)