FastQCFastQC Report
Thu 26 May 2016
SRR2099115_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099115_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260322
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTGCACATGGGACTCACTATAGTGAGTCGTATTACCGGCC27351.050621922081115No Hit
AGTCTGCAGTCTTACAGTCCGACGATCTGTATAGTCTGCATTT14540.5585390401118615No Hit
GATCTGCACATGGAGTGAGTCGTATTACCGGCCGACGATCCAT13210.5074484676669663No Hit
AGTCTGAATCTTAATAAGCACGACGTAAGTCACTTTTAATATT8260.31729934465777No Hit
AGTATGCCGAGTGCTGGAGGTGGCAACGCCAATCAGCAACGAC7650.29386682646875795No Hit
AGTCTGCACTAATACTTTCGTTTAATTTAAGATTTCTACGCTT6880.2642880740006607No Hit
AGTCTGCACTCTGGGAAAAGGGGAAAAGCAAGTGGGAGCCTGT5250.20167331228248092No Hit
AGTCTGCATTTGTATCAGGGTGGGATCAGCTCTTACAACTTCT5200.19975261407026682No Hit
AGTCTGCACCGGTGGATACCCAGCTCAGAGAAGAAGAGGAGGA4950.1901491230091963No Hit
AATCAGCATCAGACATGGATCTGACTTTTTTTGGTTTCTGAGA4840.18592358694232528No Hit
AGTCTGCACGGAGGTGCAGTGGCTCACGCCTATAATCCTTGCT4770.18323460944522554No Hit
AGTCTGGGCTTCAGGCGGCCCGCGCGGCCAAGGAACTGGAAGA4650.17862493373591168No Hit
AGTCTGCAGTATTAAATCACGGAAAATGAGAAATACACACTTT4570.17555181659636912No Hit
AGTCTGCAGTGTAAGTTTGGGAATGGATATCTCAGACCCTACA4360.16748488410506987No Hit
AGTCTGGGCTTCAGCGGCCCGCGCGGCCAAGGAACTGGAAGAC4230.1624910687533132No Hit
AGTCTGCATTACGCCGGCGGTGTGCAGGCAGAGCAGCGGCGTG4200.16133864982598475No Hit
AGTCTGGGCCAGGGAATCTACCCATCTCTACGGAATAACGCAT3940.1513510191224714No Hit
AGTCTGCACATCAAGTGACGTCCATGAGCGGTCGAAGCGTTGG3890.1494303209102573No Hit
AGTGTTCAGTCACGAGCAAGTGTGACATCCATTTATGCAATCT3850.147893762340486No Hit
AATCTGCACATGGGCAGAAAGAGTGTCTCAAACCTGGTATATA3730.14328408663117218No Hit
AGTCTGCACGTGGAGTCACTGCAGGCCAACGTGCAGCGCCTGA3660.14059510913407242No Hit
AATCTGCGCAGTAGTCTACGAGCCCTCAGCGGTGATTCAACTG3570.13713785235208703No Hit
AGTCTGCACGCTCCCGACGATCATTTCAGTCTGCATTTTTTTT3350.12868678021834498No Hit
AGTCTGCAGTCGTACTCCATAAGCTCCATACCAATCCCCATCA3310.1271502216485737No Hit
AGTCTGCAGCTCAAGAAACCATCACGAGGATTGGTGAACAAAG3300.12676608200613088No Hit
AGTCTGGGCCAGGCATCTCTACGGAATAACGCATGGAAACGTG3010.11562603237528907No Hit
AGTCTGCATACTACAGTCCGACGATCAATGCAGACTCCATAGA2870.11024807738108956No Hit
AGTCGGCCGGTCGAGCATGCCTCACAGCACACCTTCACAGGCT2680.10294942417467597No Hit
AGTCTGCAGTATACTACAGTCCGACGATCTTTGGCTAGATCGT2660.10218114488979033No Hit
AGTCTGCATCGGTGCCAGCTGGGTTCAGTGGCTCATGCCTGTA2630.10102872596246187No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAT251.2264942E-437.02306714
TCGATCT200.001833698837.02306713
GCGGCCG200.001833698837.02306713
GTGACGT452.59206E-937.01595315
TCTACCC403.799323E-837.0159517
CCAAGTG200.001835431137.0159515
GGGTTAC403.799323E-837.0159517
AGTTTAG308.284624E-637.001738
ATTCGCA308.284624E-637.001738
AGTGGCG251.2306645E-437.001738
ATGTCCC308.284624E-637.001738
GGGCGAT251.2306645E-437.001739
ACGTGAG308.284624E-637.0017312
CGAATCG251.2306645E-437.0017312
CCGGTCT308.284624E-637.001739
CGCAATT251.2306645E-437.0017311
AGGGCGA251.2306645E-437.001738
AGCACCG200.001838899237.0017288
CTTAAGG200.001838899237.0017289
AGCGTTA200.001838899237.0017288
Produced by FastQC (version 0.11.5)