FastQCFastQC Report
Thu 26 May 2016
SRR2099114_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099114_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163372
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACAGTAGGATACCAACACCCTCCGCAGCTACTTCTGCCGA17711.084029086991651No Hit
GCTACAGTCACCTAACCAAGGGCGAGGGACCAAAGAGAAAGAA9150.5600714932791421No Hit
GCTACAGTGGTTTTATTTGTGTTCTCAGACACTAAGCTAGGTG7320.4480571946233137No Hit
TCGACAGTCGGGGGAGGAAGGCAAACATGCGCGCCGACAAGAT6790.41561589501260926No Hit
GCTACAGTCACGTCTACAGTTTTTTTTTTTTTTTTTTTTTTTT6640.40643439512278723No Hit
GCTACAGTCTGCTCAGGCTGAGTGCTGCGGCGCGATCCTTGCT5890.3605268956736773No Hit
TCGACAGTATTTTGTGGATTTGTGGCGAGTACCTACATGAGGG5660.34644859584261684No Hit
ACGACAGAGCCTGTGCTTATCTGGAGAAACTCGCCCGCTGGAT4930.30176529637881644No Hit
TCGTCAGTACCTCTGGCAGCGCACGCAGGGGGGCATGTTGTCG4370.26748769679014767No Hit
GCCACAGTTACTTGTCTGGATTCTGGCGCGCATTCTCCTGACG4330.2650392968195284No Hit
GCTACAGTGCGGTGTGGCGTATCAAACCTCAGCCGAACCGGCG4290.26259089684890924No Hit
ACTACAGTGCCTTAAAGGGGAAGGGGTCTTGCTGGGTGGGATC4270.26136669686359965No Hit
TCCCCAGTTTTTTGGACTGACCTTAAGCGATTACGACCGCCTG4200.25708199691501604No Hit
GCTACAGTCATTTCTGCTCATGCCCACAGCCTCGAGGGGAAAA4050.24790049702519404No Hit
GCTACAGTCCGGTCGCGAAGTTGTTTTACTCTTTCCTTAATCT3740.22892539725289526No Hit
GCTACAGTGTCTTGACAAAGTGTCTTAAACAAACGTCTTGGCA3660.22402859731165686No Hit
TCGACATAGACCGGGCCGCTTTATGTGTTGATCGGGAATTTTG3650.223416497319002No Hit
TCGACAGTATTTTGGTGGATTTGTGGCGAGTACCTACATGAGG3620.22158019734103765No Hit
TCAACAATGTACTGGTCAGGCTTCGCTGTTCTGTCTCAAGGTC3570.2185196973777636No Hit
GCTACAGTCACCTACCAAGGGCGAGGGACCAAAGAGAAAGAAG3480.21301079744387041No Hit
TCGACAGTCCGAGTACAGTCCGACGATCTTCGTTCGACAGTAG3480.21301079744387041No Hit
GCTACAGTAACCTGTCTGTATTGTGCAACTTCCTTTAAATTGT3450.211174497465906No Hit
CTGACAGTCTCCAGGATGAACTAAGTTCTAAACGAATGTCACT3440.21056239747325123No Hit
TCCACACTATGGGCCGCAAGGTGAAGGTGCAGGTGCGCAGCGA3190.19525989765688123No Hit
GCTACAGTTGCATGTAAGTAACTAGAATGTCACTTTGTTGGAA3130.19158729770095242No Hit
GCTACAGTCCGGTGTATTTTCCTTTTCAACTTTAACTTTTTGT3000.18362999779644001No Hit
GCTACAGTACCCTGACGATCACCATGCTACAGTGAGTCGTATT3000.18362999779644001No Hit
GCTACAGTCGGCTAGCTAGGGATCCTCTCCTGAGAAGAAAAGT2790.17077589795068923No Hit
TCGACAGTTAGTCGCGACTTCGAGCCGAACGTCGGCGTGGACA2770.1695516979653796No Hit
GCTACAGTAGACTGTCCGACGATCTGCATGCTGATCGTCGGAC2720.16649119800210563No Hit
GCTACAGTTCGATGAAAATTGATTTGGCATACGCAGCTGGAAT2710.16587909800945083No Hit
TCGACAGTACTTGCAGTCCGACGATCTATGGTCGACAGTAGAA2690.1646548980241412No Hit
TCGACATAGACCGGGGCCGCTTTATGTGTTGATCGGGAATTTT2640.16159439806086723No Hit
GCTACAGTAACATGTTGGGTTTTTTTTTTTTTTTTTTTTTTTT2570.15730969811228362No Hit
TCGACAGTTCAGTGTCCGACGATCCAGCGTCGGACTGTAGAAC2480.15180079817839043No Hit
TCGACAGCTGACCCTTCCGCCTTCACCGCTGTCACCTGTGGTA2430.14874029821511642No Hit
TCGACATAGACCGCCGGCCGCTTTATGTGTTGATCGGGAATTT2350.14384349827387802No Hit
GCTACAGTAACATGTTGGGTTTTTTTTTTTTTGTTTTGTTTTG2290.1401708983179492No Hit
TCGACTTTCCCAAGCAGGCGCGGGTAACCCGTTGAACCCCATT2280.13955879832529441No Hit
GCTACAGTGCTTTCAGTCCGACGATCATGTTGCTAAGCGATCG2260.13833459833998482No Hit
TCGACATAGACCGCGGCCGCTTTATGTGTTGATCGGGAATTTT2260.13833459833998482No Hit
GCTACAGTTCCGTTATGCATATAGGTGGAGGTGCCTTCAACTG2230.13649829836202043No Hit
GCTACAGTAACATGGTTGGGTTTTTTTTTTTTTTTTTTTTTTT2130.1303772984354724No Hit
GCTACAGTGGGTTAGCCTTGTAAACGACATTGGATGGTTCTGT2120.12976519844281761No Hit
TTGATAGTTCGACACGATCTCGAGCAATGTATCAGTGACTATC2100.12854099845750802No Hit
GCTACAGTGCGGTGGTGGCGTATCAAACCTCAGCCGAACCGGC2050.125480498494234No Hit
GCTACAGTATTGTGCTCATTAGATACTTGATCTGGGCTTAGTA2030.12425629850892442No Hit
TCGACATTTCGGCGAGTTAGAAAACGTTTGACTCTGTTCGTTT2000.12241999853096003No Hit
GCTACAGTTGAGTAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT1980.12119579854565042No Hit
TCGACAGTCAGAGGCGACTTCGAGCCGAACGTCGGCGTGGACA1970.12058369855299561No Hit
TCGACAGTCCCGCCAGATGCCCCCACCCAGGGGGCATCTGTCC1950.119359498567686No Hit
TCGACAGCTGACCTCACCGCTGTCACCTGTGGTACCTCCTTCA1940.11874739857503122No Hit
GCTACAGTTTTGTAATTTGGGTAAAAAGCCACCCAAATTCACA1910.1169110985970668No Hit
GCTACAGTGATATTTCTACAGTCCGACGATCGTCGGACTGTAG1910.1169110985970668No Hit
TCAACAATGTACTGTCAGGCTTCGCTGTTCTGTCTCAAGGTCG1850.11323849864113802No Hit
GCCACAGTTACTTGGTCTGGATTCTGGCGCGCATTCTCCTGAC1840.11262639864848321No Hit
TCGGCGGTACGGGCCTTCCGGTTGAACCGGAAGGATTTCGAAA1800.11017799867786401No Hit
GCTACAGTTGGATCGAGTGCCACACATGGTGGCACTCGTGTCT1770.10834169869989962No Hit
GCTACAGTTACCTAGATCACGAGGTCAAGAGATCGAGACCATC1760.10772959870724481No Hit
GCTACAGTGCGCTCGTCCGACTGTAGAACTCTGAACCCCTATA1750.10711749871459002Illumina DpnII expression Adapter 1 (95% over 23bp)
TCGGCGGTACGGGCGGTTGAACCGGAAGGATTTCGAAAATGGC1750.10711749871459002No Hit
GCTACAGTAGGTTGGGGGGCGGGCGCCGGCGGCTTTGGTGACT1730.10589329872928042No Hit
GCTACAGTAACCTAGATGTCTGTATTGTGCAACTTCCTTTAAA1700.10405699875131601No Hit
GCTACAGTTGGATCACACATGGTGGCACTCGTGTCTCTAGTTG1670.10222069877335162No Hit
GCTACAGTAACCTCCAAGATGTCTGTATTGTGCAACTTCCTTT1660.10160859878069681No Hit
TCGACAGTAGATAGTCCGACGATCCGACGTCGGACTGTAGAAC1660.10160859878069681No Hit
TCAACCGTCAGCAGGTTCCAGTAGGTGGGCTTCAAACTGTACT1660.10160859878069681No Hit
TCGGCGGTACGGGTCCTTCCGGTTGAACCGGAAGGATTTCGAA1640.1003843987953872No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGCA200.001817080737.0834936
CCGCCTG650.037.0834937
CTTAGTA700.037.0834937
TATTTGG403.7749487E-837.02666513
TGTCATA200.001830823537.0266658
CTAGATG308.22756E-637.02666512
GTGTTAA355.5604505E-737.02666512
TAGAGTG308.22756E-637.0266658
TCGTTTG308.22756E-637.0266658
CTGTCCG403.7749487E-837.02666512
GATACAG251.2237896E-437.02666511
CGAACGA200.001830823537.02666510
TACTTGG308.22756E-637.0266659
TCCGATC251.2237896E-437.02666512
ACTTGGT251.2237896E-437.02666510
TCACATT200.001830823537.0266658
GCGGTAG200.001830823537.0266659
GCGAAGA251.2237896E-437.0266659
GGGTCCG251.2237896E-437.02666511
TAGGTTC750.037.0266658
Produced by FastQC (version 0.11.5)