FastQCFastQC Report
Thu 26 May 2016
SRR2099113_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099113_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110493
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACAGTAGGATACCAACACCCTCCGCAGCTACTTCTGCCGA11201.0136388730507815No Hit
GCTACAGTCACCTAACCAAGGGCGAGGGACCAAAGAGAAAGAA5980.5412107554324709No Hit
GCTACAGTGGTTTTATTTGTGTTCTCAGACACTAAGCTAGGTG4790.4335116251708253No Hit
TCGACAGTCGGGGGAGGAAGGCAAACATGCGCGCCGACAAGAT4640.4199361045496095No Hit
GCTACAGTCACGTCTACAGTTTTTTTTTTTTTTTTTTTTTTTT3990.3611088485243409No Hit
TCGACAGTATTTTGTGGATTTGTGGCGAGTACCTACATGAGGG3720.3366729114061524No Hit
GCTACAGTCTGCTCAGGCTGAGTGCTGCGGCGCGATCCTTGCT3710.33576787669807134No Hit
ACGACAGAGCCTGTGCTTATCTGGAGAAACTCGCCCGCTGGAT3380.3059017313313966No Hit
TCCCCAGTTTTTTGGACTGACCTTAAGCGATTACGACCGCCTG3280.296851384250586No Hit
GCCACAGTTACTTGTCTGGATTCTGGCGCGCATTCTCCTGACG2980.2697003430081544No Hit
ACTACAGTGCCTTAAAGGGGAAGGGGTCTTGCTGGGTGGGATC2820.2552197876788575No Hit
GCTACAGTGCGGTGTGGCGTATCAAACCTCAGCCGAACCGGCG2820.2552197876788575No Hit
TCGTCAGTACCTCTGGCAGCGCACGCAGGGGGGCATGTTGTCG2810.25431475297077644No Hit
GCTACAGTCATTTCTGCTCATGCCCACAGCCTCGAGGGGAAAA2640.2389291629333985No Hit
TCAACAATGTACTGGTCAGGCTTCGCTGTTCTGTCTCAAGGTC2530.22897378114450687No Hit
TCGACAGTATTTTGGTGGATTTGTGGCGAGTACCTACATGAGG2430.21992343406369635No Hit
GCTACAGTGTCTTGACAAAGTGTCTTAAACAAACGTCTTGGCA2370.21449322581521No Hit
TCGACATAGACCGGGCCGCTTTATGTGTTGATCGGGAATTTTG2360.21358819110712896No Hit
GCTACAGTCCGGTCGCGAAGTTGTTTTACTCTTTCCTTAATCT2250.20363280931823738No Hit
TCGACAGTCCGAGTACAGTCCGACGATCTTCGTTCGACAGTAG2240.20272777461015629No Hit
CTGACAGTCTCCAGGATGAACTAAGTTCTAAACGAATGTCACT2210.20001267048591312No Hit
GCTACAGTAACCTGTCTGTATTGTGCAACTTCCTTTAAATTGT2140.19367742752934575No Hit
GCTACAGTCACCTACCAAGGGCGAGGGACCAAAGAGAAAGAAG2120.19186735811318362No Hit
TCCACACTATGGGCCGCAAGGTGAAGGTGCAGGTGCGCAGCGA2090.18915225398894048No Hit
GCTACAGTACCCTGACGATCACCATGCTACAGTGAGTCGTATT2070.18734218457277838No Hit
GCTACAGTTGCATGTAAGTAACTAGAATGTCACTTTGTTGGAA2000.18100694161621098No Hit
GCTACAGTTCGATGAAAATTGATTTGGCATACGCAGCTGGAAT1940.17557673336772464No Hit
GCTACAGTCCGGTGTATTTTCCTTTTCAACTTTAACTTTTTGT1870.16924149041115727No Hit
GCTACAGTCGGCTAGCTAGGGATCCTCTCCTGAGAAGAAAAGT1760.15928610862226567No Hit
TCGACATAGACCGGGGCCGCTTTATGTGTTGATCGGGAATTTT1730.1565710044980225No Hit
TCGACAGTACTTGCAGTCCGACGATCTATGGTCGACAGTAGAA1710.1547609350818604No Hit
GCTACAGTTGAGTAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT1690.1529508656656983No Hit
TCGACAGTTAGTCGCGACTTCGAGCCGAACGTCGGCGTGGACA1670.15114079624953616No Hit
GCTACAGTAGACTGTCCGACGATCTGCATGCTGATCGTCGGAC1610.14571058800104986No Hit
TCGACAGTTCAGTGTCCGACGATCCAGCGTCGGACTGTAGAAC1600.14480555329296876No Hit
TCGACATAGACCGCCGGCCGCTTTATGTGTTGATCGGGAATTT1590.14390051858488773No Hit
GCTACAGTTCCGTTATGCATATAGGTGGAGGTGCCTTCAACTG1520.13756527562832033No Hit
TCGACATAGACCGCGGCCGCTTTATGTGTTGATCGGGAATTTT1480.13394513679599612No Hit
TCGACTTTCCCAAGCAGGCGCGGGTAACCCGTTGAACCCCATT1460.13213506737983402No Hit
TCGACAGTCCCGTGGGAAAGGCCAGGCTGGGGCACCTGGTCCA1440.1303249979636719No Hit
TCGACAGCTGACCCTTCCGCCTTCACCGCTGTCACCTGTGGTA1410.12760989383942875No Hit
GCTACAGTGCGGTGGTGGCGTATCAAACCTCAGCCGAACCGGC1360.12308472029902347No Hit
GCTACAGTAACATGTTGGGTTTTTTTTTTTTTGTTTTGTTTTG1350.12217968559094242No Hit
TCGACAGCTGACCTCACCGCTGTCACCTGTGGTACCTCCTTCA1350.12217968559094242No Hit
GCTACAGTGGGTTAGCCTTGTAAACGACATTGGATGGTTCTGT1350.12217968559094242No Hit
GCTACAGTGCTTTCAGTCCGACGATCATGTTGCTAAGCGATCG1330.1203696161747803No Hit
GCCACAGTTACTTGGTCTGGATTCTGGCGCGCATTCTCCTGAC1330.1203696161747803No Hit
GCTACAGTAACATGTTGGGTTTTTTTTTTTTTTTTTTTTTTTT1330.1203696161747803No Hit
TTGATAGTTCGACACGATCTCGAGCAATGTATCAGTGACTATC1320.11946458146669925No Hit
TCAACCGTCAGCAGGTTCCAGTAGGTGGGCTTCAAACTGTACT1290.11674947734245608No Hit
GCTACAGTAACATGGTTGGGTTTTTTTTTTTTTTTTTTTTTTT1280.11584444263437503No Hit
GCTACAGTATTGTGCTCATTAGATACTTGATCTGGGCTTAGTA1280.11584444263437503No Hit
TCGACATTTCGGCGAGTTAGAAAACGTTTGACTCTGTTCGTTT1270.11493940792629398No Hit
GCTACAGTGCATTGAGAAAATATGAGATTTGTCTGTGGCTTTG1220.1104142343858887No Hit
TCGACAGTCCCGCCAGATGCCCCCACCCAGGGGGCATCTGTCC1160.10498402613740238No Hit
TCCACCGTCAGTCATCCAGTCCATTGCTTTCGAACGGTGCAGA1160.10498402613740238No Hit
GCTACAGTAGGTTGGGGGGCGGGCGCCGGCGGCTTTGGTGACT1160.10498402613740238No Hit
GCTACAGTGATATTTCTACAGTCCGACGATCGTCGGACTGTAG1160.10498402613740238No Hit
TCGACAGTCAGAGGCGACTTCGAGCCGAACGTCGGCGTGGACA1150.10407899142932131No Hit
GCTACAGTTTTGTAATTTGGGTAAAAAGCCACCCAAATTCACA1150.10407899142932131No Hit
TCAACAATGTACTGTCAGGCTTCGCTGTTCTGTCTCAAGGTCG1130.10226892201315921No Hit
GCTACAGTTTGATCCTCAGCCACCTTCCCAATGCAGTGCTCAG1130.10226892201315921No Hit
GCTACAGTTACCTAGATCACGAGGTCAAGAGATCGAGACCATC1130.10226892201315921No Hit
GCTACAGTTGGATACACATGGTGGCACTCGTGTCTCTAGTTGC1120.10136388730507814No Hit
TCTACCGTGTACGGGCGGAAGAAGTCCACGCGGCCCGGCTTCG1120.10136388730507814No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTTT200.001832205237.01041819
AAGTAAC251.2243813E-437.01041816
GGCAGCG501.7462298E-1037.01041815
CTCACCG200.001832205237.01041813
GTCTGTG251.2243813E-437.01041831
CGAGTGC200.001832205237.01041814
AAATAGC200.001832205237.01041817
TAGCGCC200.001832205237.01041813
ATAGCAA200.001832205237.01041819
ATGCTTA200.001832205237.01041815
ATCACCT200.001832205237.01041833
GCGCACG501.7462298E-1037.01041819
AATGCTG200.001832205237.01041817
GCCTCTT200.001832205237.01041819
GCAGCGC501.7462298E-1037.01041816
AATAGCA200.001832205237.01041818
AAAGGTT200.001832205237.01041817
CTTAGTA251.2243813E-437.01041837
TGTAAGT251.2243813E-437.01041813
TATAAAG200.001832205237.01041813
Produced by FastQC (version 0.11.5)