FastQCFastQC Report
Thu 26 May 2016
SRR2099112_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099112_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155837
Sequences flagged as poor quality0
Sequence length42
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGCTAGAATACGACTCACTATAGTGAGTCGTATTACCGGC39442.5308495415081143No Hit
ACTGCTAGCAACACGATCCCAAACTACTAGTTTTTTTTTTTT33432.145190166648485No Hit
ACTGCTACAGTGGGTCTCCCTGCAGGGGAGATACAACTAATT26471.698569659323524No Hit
ACTACGAGATCACCGAGTCGCTGTAATGCTGGGCCGGCATGC11510.7385922470273426No Hit
ACTGCTTATTAGCCGACGATCTTCTACTGCTAGTTTTTTTTT9820.6301456008521724No Hit
ACTGCTACAGTGGGGTCTCCCTGCAGGGGAGATACAACTAAT9390.6025526672099695No Hit
ACGGCGAGACCGCCCGGGCACCTCAGCCCCAAAACACATCAG8620.5531420651064896No Hit
ACTGCTATCTGTCAGTCCGACGATCTAGGACTGTAGAACTCT7970.5114318165775779No Hit
GCTGATAGTCGAGGGCTGATCATTAACTATCCGCTGGATGAC5800.37218375610413446No Hit
ACTGCTAGAGACATGAAGGCATGCATTTTACATAGACGTCTC4850.3112226236388021No Hit
ACGGCGAGACCGTCCCGGGCACCTCAGCCCCAAAACACATCA4740.30416396619544783No Hit
ACTGCTCGAATTGGTGGAACGCCCTGCGTCGGTGGTGAAGGA4730.30352227006423377No Hit
ACTGCTTGAAGTGCCGGTGCCACCACCATCGTCGCCGTTGAC4720.30288057393301976No Hit
ACGGCGAGACCGGCCCGGGCACCTCAGCCCCAAAACACATCA4340.2784961209468868No Hit
ACTGACAGCTCCGGATGAACTAAGTTCTAAACGAATGTCACT3460.22202686140005262No Hit
ACTGCTAGGCTTGTTACAGGAGGTATAGAGGACTATGGGCCA3450.22138516526883859No Hit
ACTGCTAGCGTGAAATCACTAAAAAACGTGAAAAATAAGAAA2810.180316612871141No Hit
ACTGCTAGTTTGCAGTCCGACGATCAGGGACTGCTAGCAGTG2790.17903322060871296No Hit
ACCGCTATAGACTCCGACGATCCGCGACTGCTAGTTTTTTTT2730.1751830438214288No Hit
ACTGCAAAGTCAGTCCGACGATCCATGTAGGACTGTAGAACT2680.17197456316535867No Hit
ACTGCTAGCTTAGTTCTACAGTCCGACGATCGTGGACTGCAG2670.17133286703414466Illumina Small RNA Adapter 1 (95% over 21bp)
ACTGCTAGAATATCGACTCACTATAGTGAGTCGTATTACCGG2630.16876608250928857No Hit
GATGCTAGCATGATCGGCGCAATGCGTCAGGATGTTCGATAC2340.15015689470408183No Hit
ACTGCTAGAATAATTACCGGCCGACGATCAAAAACTAGCAGT2340.15015689470408183No Hit
ACTGCTAGAATACCGGCCGACGATCAAAAACTAGCAGTTATT2330.1495151985728678No Hit
ACTGCTAGAATACCGACTCACTATAGTGAGTCGTATTACCGG2330.1495151985728678No Hit
ACTACGAGATCATCCGAGTCGCTGTAATGCTGGGCCGGCATG2250.1443816295231556No Hit
ACTGCTAGGTCGGGGAAAGGAGAAAACACAATGTCTTTGCCT2120.13603957981737327No Hit
ACTGCCTGGCGAGCTCTGTCGCCCAGGCTGGAGTGCAGTGGC2090.1341144914237312No Hit
ACTGCTAGAATAGCGACTCACTATAGTGAGTCGTATTACCGG2040.1309060107676611No Hit
ACTGCTACAGTGTGGTCTCCCTGCAGGGGAGATACAACTAAT1990.12769753011159096No Hit
ACTGGAAGTCAGGAAAGATAAACATGGATCTGACTTTTTTTT1990.12769753011159096No Hit
ACTACGAGATCAGCCGAGTCGCTGTAATGCTGGGCCGGCATG1970.1264141378491629No Hit
ACTGCTAGAACAACGACTCACTATAGGGAGTCGTATTACCGG1910.12256396106187877No Hit
TCAGCTAGGTACGCCAAGGGCACCCCAGTCTAACTGCCAGGA1900.12192226493066474No Hit
ACTGGTAACAGGCCTAGACCAAGGCTGCAGGTCACCTCAGTT1870.11999717653702266No Hit
ACTGCTAGATGGAAAGACACAGACTGGCAAGTTGGATAAAGA1850.11871378427459461No Hit
ACTGCTAGTAGATGATGGACCCACTTCCATGACTAGCTGCAA1780.11422191135609644No Hit
ACTGCTAGTTCTGAAAAGGCTTGCCAGTCCATTTATCCTCTC1780.11422191135609644No Hit
ACTGCTAGTATTCATTAGCAGTCCGGCTTACAGCTAACATTA1740.11165512683124032No Hit
ACTGCTAGAATAAGTGAGTCGTATTACCGGCCGACGATCAAA1710.10973003843759827No Hit
ACTGCTAAAGCCAACAGCTGTGCTGAAATTTTTTTTTATCAT1710.10973003843759827No Hit
ACTGCTACAGTGCGGTCTCCCTGCAGGGGAGATACAACTAAT1650.10587986165031411No Hit
ACTGCTACAGTGAGGTCTCCCTGCAGGGGAGATACAACTAAT1630.10459646938788605No Hit
ACTGCTCTCTGGTATATGCCACTCCCTGTCATGCCCAAGGTA1620.10395477325667205No Hit
ACTGCTAGCCACGGGCAATGGCAGACCAGATTGTGGGTCTCC1580.10138798873181594No Hit
ACTGCTAGGTCCCTACAGTCCGACGATCGTGGACTGTAGAAC1580.10138798873181594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAC404.742469E-836.0772236
CGGGCGT200.002079533436.0772236
ATCCAGA200.002079533436.0772235
ACGTCTC551.6370905E-1136.0772236
TCATACA200.002079533436.0772236
AGCGTTG251.4391058E-436.0192767
CTAGACG1600.036.0192765
AGCGTAC200.002096014636.0192767
TAGAGGC200.002096014636.0192766
AGTCCCG200.002096014636.0192767
TACCCCA502.3646862E-1036.0192766
CGTATTC200.002096014636.0192767
CTCCGGG200.002096014636.0192769
TGAAGTG404.8099537E-836.0192767
TAGACGC404.8099537E-836.0192766
TAGACCG200.002096014636.0192766
TAGGTTG502.3646862E-1036.0192766
ACCGCCC1050.036.0192769
TAGGTGT251.4391058E-436.0192766
TAGGTGC200.002096014636.0192766
Produced by FastQC (version 0.11.5)