FastQCFastQC Report
Thu 26 May 2016
SRR2099111_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099111_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114472
Sequences flagged as poor quality0
Sequence length42
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGCTAGAATACGACTCACTATAGTGAGTCGTATTACCGGC24882.173457264658606No Hit
ACTGCTAGCAACACGATCCCAAACTACTAGTTTTTTTTTTTT22211.9402124537004684No Hit
ACTGCTACAGTGGGTCTCCCTGCAGGGGAGATACAACTAATT17161.4990565378433154No Hit
ACTACGAGATCACCGAGTCGCTGTAATGCTGGGCCGGCATGC7850.6857572157383465No Hit
ACTGCTTATTAGCCGACGATCTTCTACTGCTAGTTTTTTTTT6530.5704451743657838No Hit
ACTGCTACAGTGGGGTCTCCCTGCAGGGGAGATACAACTAAT6160.5381228597386261No Hit
ACGGCGAGACCGCCCGGGCACCTCAGCCCCAAAACACATCAG5720.49968551261443844No Hit
ACTGCTATCTGTCAGTCCGACGATCTAGGACTGTAGAACTCT5030.4394087637151443No Hit
GCTGATAGTCGAGGGCTGATCATTAACTATCCGCTGGATGAC3790.3310853309106157No Hit
ACTGCTAGAGACATGAAGGCATGCATTTTACATAGACGTCTC3340.29177440771542384No Hit
ACGGCGAGACCGTCCCGGGCACCTCAGCCCCAAAACACATCA3150.27517646236634286No Hit
ACTGCTCGAATTGGTGGAACGCCCTGCGTCGGTGGTGAAGGA3090.2699350059403173No Hit
ACTGCTTGAAGTGCCGGTGCCACCACCATCGTCGCCGTTGAC3070.2681878537983088No Hit
ACGGCGAGACCGGCCCGGGCACCTCAGCCCCAAAACACATCA2910.25421063666224053No Hit
ACTGCTAGGCTTGTTACAGGAGGTATAGAGGACTATGGGCCA2340.20441680061499753No Hit
ACTGACAGCTCCGGATGAACTAAGTTCTAAACGAATGTCACT2220.1939338877629464No Hit
ACTGCTAGGTCGGGGAAAGGAGAAAACACAATGTCTTTGCCT2120.18519812705290378No Hit
ACCGCTATAGACTCCGACGATCCGCGACTGCTAGTTTTTTTT1910.16685302956181425No Hit
ACTGCTAGTTTGCAGTCCGACGATCAGGGACTGCTAGCAGTG1900.16597945349080998No Hit
ACTGCTAGCGTGAAATCACTAAAAAACGTGAAAAATAAGAAA1890.16510587741980573No Hit
ACTGCTAGCTTAGTTCTACAGTCCGACGATCGTGGACTGCAG1830.15986442099378012Illumina Small RNA Adapter 1 (95% over 21bp)
GATGCTAGCATGATCGGCGCAATGCGTCAGGATGTTCGATAC1820.15899084492277588No Hit
ACTGCTAGTTCTGAAAAGGCTTGCCAGTCCATTTATCCTCTC1770.15462296456775457No Hit
ACTGCAAAGTCAGTCCGACGATCCATGTAGGACTGTAGAACT1700.1485079320707247No Hit
ACTGCTAGAATATCGACTCACTATAGTGAGTCGTATTACCGG1660.14501362778670768No Hit
ACTGCTAGCCACGGGCAATGGCAGACCAGATTGTGGGTCTCC1580.13802501921867358No Hit
ACTACGAGATCATCCGAGTCGCTGTAATGCTGGGCCGGCATG1490.1301628345796352No Hit
ACTGCTAGAATACCGGCCGACGATCAAAAACTAGCAGTTATT1480.12928925850863093No Hit
ACTGCTAGTGCGGAGCAAGCAGGAACTGAGCAAGCAGGGCTG1480.12928925850863093No Hit
ACTACGAGATCAGCCGAGTCGCTGTAATGCTGGGCCGGCATG1460.12754210636662242No Hit
ACTGCTAGAATACCGACTCACTATAGTGAGTCGTATTACCGG1460.12754210636662242No Hit
TCAGCTAGGTACGCCAAGGGCACCCCAGTCTAACTGCCAGGA1460.12754210636662242No Hit
ACTGCTAGAATAATTACCGGCCGACGATCAAAAACTAGCAGT1430.12492137815360961No Hit
ACTGCCTGGCGAGCTCTGTCGCCCAGGCTGGAGTGCAGTGGC1410.12317422601160108No Hit
ACTGCTAGAATAGCGACTCACTATAGTGAGTCGTATTACCGG1340.11705919351457125No Hit
ACTGCTAGAACAACGACTCACTATAGGGAGTCGTATTACCGG1270.11094416101754141No Hit
ACTGCTAGATGGAAAGACACAGACTGGCAAGTTGGATAAAGA1240.10832343280452862No Hit
ACTGCTACAGTGTGGTCTCCCTGCAGGGGAGATACAACTAAT1210.10570270459151583No Hit
ACTGCTAGTAGATGATGGACCCACTTCCATGACTAGCTGCAA1210.10570270459151583No Hit
ACTGGAAGTCAGGAAAGATAAACATGGATCTGACTTTTTTTT1190.1039555524495073No Hit
ACTGCTAGATGGAAGAAACTAGGGGAATGGGTAGGCCTCTGT1180.10308197637850303No Hit
ACTGCTAGAATAGAAAAGGCTTGCCAGTCCATTTATCCTCTC1170.10220840030749877No Hit
ACTGCTAAAGCCAACAGCTGTGCTGAAATTTTTTTTTATCAT1160.10133482423649452No Hit
ACTGCTAGCGTCGGGAAAGGAAAGTGGAGAGATTGAGCCTGT1160.10133482423649452No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAACC200.002095206236.01398535
GACGTCT502.3464963E-1036.01398535
ACCTCAT200.002095206236.01398533
ATCGTCC251.4379626E-436.01398536
TTCCAAC200.002095206236.01398535
AGCGAAC200.002095206236.01398534
ACGTCTC502.3464963E-1036.01398536
TCATACA200.002095206236.01398536
ACTGCAA950.035.9982571
AGACCGC750.035.9982577
CTAGCGC1500.035.9982575
TCCGGCT200.002099708235.99825322
TCCGGAT356.908558E-735.99825310
CTGCTTA1400.035.9982532
CTTGTAG356.908558E-735.9982532
ACAACGA200.002099708235.99825310
CTAGATC850.035.9982535
CTAGATA850.035.9982535
AGCGTTG251.441667E-435.9982537
TATCACG200.002099708235.9982537
Produced by FastQC (version 0.11.5)