FastQCFastQC Report
Thu 26 May 2016
SRR2099110_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099110_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234429
Sequences flagged as poor quality0
Sequence length47
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTGGTAGATGTCCTCGGCAATCAGCGGACCAGCCTTCGGATCGA9560.4077993763570206No Hit
GATCTGACAATTTCAAAACCGAACGTTACTCAATGGGACCAAGTCGC8770.3741004739174761No Hit
GATCTGACCGTAACAAGCGGCTCGGCCCGGGCCGGCATGGACGCCAG8220.35063921272538806No Hit
GATCTGACTGAAATCAAAGGGAGTCGGGTTCAGATCCCCGAATCCGG7680.3276045199186108No Hit
AATCTGTCAACGTGCATTGGAAGTGCATCACTCCAAGTGCGGCGCAC7410.3160871735152221No Hit
GATCTGTCAAGAGAACATAGAAGAGCCTTTAAGCAAAGCTTTCTAAG4340.18513067922483994No Hit
GATCTGACAACGAGAAACACGACGCTACTCAGACTACCCAGATGCTT3970.16934764896834434No Hit
GATCTGACAAGATAGAAGACGCGGCGCGTGCGCGGGCGGGGCCCGCG3830.16337569157399467No Hit
GATCTGACCATTAAAATGGAAGCCCTGAGGTTCATCCCAGCAACGAA3640.15527089225309154No Hit
GATCTGACAGCAACTAGGAGGATTGCTTGAGCCGAAGTGGTTGAGGC3230.1377815884553532No Hit
GATCTGACAAGTGTACCAGAGGGTTTAACATCTACTGTAGTTATGAG3200.13650188329942115No Hit
GATCTGACGGCGTAAATGTCCGACGATCTCAGATGCATGAACATGGA3160.1347956097581784No Hit
GATCTTAGACAGCCAAGGTCCGACGATCTCAGAGCATCACCCATGGA3090.13180963106100355No Hit
GATCTGACAAATAAAAAAGTCCGACGATCTCAGCCAGATGAGCATGG3010.128397083978518No Hit
GATCTGACATTTTAAACGAAACATTTGCAGCCGTCTGTGGAACTAAA3010.128397083978518No Hit
GATCTGACTTTAGATATCTAACGGAAATGAACTATTCCAAATATGGT2980.12711737882258595No Hit
GATCTGACAACAAATAAGATCTGACTTTTTTTTTTTCTGAGATCGTC2910.1241314001254111No Hit
GATCTGACGCTAAAAAGACCAAAAATAATGAACTTAAAACAAAAATA2800.1194391478869935No Hit
GATCTGACACAAAAAAAAAGCATGGATCTGACTTTTTTTTTTTTTTT2750.11730630596044005No Hit
GATCTGACCAAGCCTCGTGACACATGAGCAAAAGCCCACTTCGCCAT2720.11602660080450797No Hit
GATCTGACAAATACGGAATACGACTCAATAAAGTCAGATCCATGTCC2710.1156000324191973No Hit
GATCTGACGTGCTAGCAAGTCCGACGATCTCAGCAAATGAAACATGG2630.11218748533671176No Hit
GATCTGACAGACAGTCACAGTCCGACGATCTCAGGATAAACAACATG2550.10877493825422622No Hit
GCTCTGACCAGAAACAGGAAAACAGTACATGGATCTGACTTTTTTCT2520.10749523309829415No Hit
GATCTGGTAGATGTCCTTCGGCAATCAGCGGACCAGCCTTCGGATCG2470.10536239117174069No Hit
GATCTGACAGCAACTAGGGAGGATTGCTTGAGCCGAAGTGGTTGAGG2430.10365611763049794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACGG10950.041.0123834
TCTGCCG3150.041.012383
ATCTCGC256.7104455E-541.012382
TGCCCTA304.085461E-641.012385
TGCCCGG304.085461E-641.012385
TGCCCGC600.041.012385
GTCTGAT304.085461E-641.012382
TCCTGAC256.7104455E-541.012382
TGACGTC600.041.012385
TGACGTA1900.041.012385
TATGACA750.041.012383
TGACCGG850.041.012385
TCTGTGA256.7104455E-541.012383
TCTGTCC256.7104455E-541.012383
TGACACC2400.041.012385
TCGCTCT256.7104455E-541.012385
TCTCGCT256.7104455E-541.012383
GATCTGC5150.041.012381
GGTCTGG256.7104455E-541.012381
CCTGACG505.820766E-1141.012383
Produced by FastQC (version 0.11.5)