FastQCFastQC Report
Thu 26 May 2016
SRR2099108_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099108_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349073
Sequences flagged as poor quality0
Sequence length47
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACGTAAAAAAATATACAGTCCGACGATCTCGACATTAAAGCCA69992.0050247369461403No Hit
GACTACGTAAGGTACCCAGGTGCCGTTCAGTGACTACGTTTTTTTTT59121.6936285533398459No Hit
GACTACGTAACCGTAAAGTCCGACGATCTCGACGTCAAGCCCAGTGA14910.4271312877249171No Hit
GACTACGTATTAGGTCGGGAATATTATTGCCCAAAAATAAAGTCAGG13340.3821550220154524No Hit
GACTACGTCGAAGTACTGGAAGCCTGCACCTGGGAAGTGTGCAGTGT10570.3028019927063967No Hit
GACTACGTTCTAAAAGGGCCGCGCTGTCATTCCAGCAGCTGACGCCC9270.2655604988068398No Hit
GACTACGTAACGGTCCTCGCGAAATCCTGCATCTGCTCCTTGGTGAT7590.21743302976741252No Hit
GACTACGTATACGAAGCAGTTCTACAGTCCGACGATCTCGAGTGCTG7390.21170356916748073No Hit
GACTACGTATGAGGCGCGTGACTACGTTTCTCCTTTCGAGATCGTCG6640.1902180919177364No Hit
GACTACGTGGAAACCTTAGCCAATCTGCTGCTTGAAGCAAGCGGCGC6220.17818622465787956No Hit
GACTACGTCATGCCATAGGGATGGGAGAAGAGAAGAGTGCGCTGTCC6180.17704033253789322No Hit
GACTACGTAAAAGGAAAGGCACTTGGTAAGGAACAAGGACAGACGAG5940.17016497981797504No Hit
GACTACGTGGAGCATTGTCCGACGATCTCGAATATGGCAGCAGTGAC5870.1681596686079989No Hit
GACTACGTTAAAAACAAAGTTCTACAGTCCGAGATCGTCGGACTGTA5060.14495535317827504No Hit
GACAATGTTCCATCATCCCGACGATCTCGAGTCCACACACAGTGACT5000.14323651499829548No Hit
GACTACGTAACCGTAAAGGTCCGACGATCTCGACGTCAAGCCCAGTG4590.13149112076843525No Hit
GACCAAGTCTGCAACTGGCAATTGCTAGCATCTCTGTATAAAAACGC4560.13063170167844548No Hit
GACTACGTGGGACAGCAATCTCGAGGCGTCGGACTGTAGAACTCTGA4490.12862639046846935Illumina DpnII expression Sequencing Primer (95% over 22bp)
GACTACGTAACCGTAAAAAAACGTAGTCACTGTTTACGGTTTCGAGA4400.12604813319850003No Hit
GACTACGTAAGGAACCCAGGTGCCGTTCAGTGACTACGTTTTTTTTT4400.12604813319850003No Hit
GACTACGTAACCTATGAACGATCTCGAGGCTCTGAACCCCTATAGTG4310.12346987592853072No Hit
GACTACGTGCAAGAGTGAACAGTGACTACGTTTTAAGAGTCGAGATC4310.12346987592853072No Hit
GACTACGTATTGCGTGCCAGTCCGACGATCTCGAGATCGTCGGACTG4290.12289692986853754No Hit
GACTACGTTGATCAGGGGTCAGTAGCAACTTGTGTCCTTGCTGGGAC4250.12175103774855116No Hit
GACTACGTGATCCAGCTGGCTGAGGACATTCTGTATCCCGAGTCTGT4110.11774041532859889No Hit
GACTACGTAAAAGGAAAGGGCACTTGGTAAGGAACAAGGACAGACGA4010.11487568502863298No Hit
GACTACGTCGCGGCTCAGGGAGAGAGAGCTAAAGAAACCTGAGCTGC3950.11315684684865344No Hit
GACTACGTCTTCCCAGTGGCAGCTCCTCACTTCCACACTGAGAATAG3870.1108650626086807No Hit
GACTACGTCTTGACGCGGGTTAGGTAAGGTTAGGTTTGGTTCAGTTA3780.10828680533871138No Hit
GACTACGTTGATGAGCAGTCCGACGATCTCGATAAAGAAAGCAGTGA3570.10227087170878299No Hit
GACTACGTCATTCTGGTGTCCGACGATCTCGATAAGAGAAGCAGTGA3570.10227087170878299No Hit
GCCTACGATTGTCTGGGTCCGACGATCAGTCAGATGAGTCAGCTTGC3540.1014114526187932No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGAT256.720731E-541.0087417
GAGCCGC304.0937266E-641.0087417
CGGTACT200.001109939741.0087417
TTCGAGC200.001109939741.0087419
ATACCTA200.001109939741.0087417
CGACGTG256.7264125E-541.0028714
ACGGACC256.7264125E-541.0028713
GCAATCT256.7264125E-541.0028715
GCGGTTA304.097761E-641.00286515
TAACGCC200.001110722441.00286514
TATGCCT352.5099143E-741.00286512
AACGGCC200.001110722441.00286516
ATGTTAC304.097761E-641.00286514
ACGACAT200.001110722441.00286513
AGGTCGG1700.041.00286512
GTCGCAC459.549694E-1040.9969947
CGTATAG1250.040.9969946
TAAAGCG256.732098E-540.99699411
CGCGGCT506.002665E-1140.9969949
CCGATAT256.732098E-540.99699411
Produced by FastQC (version 0.11.5)