FastQCFastQC Report
Thu 26 May 2016
SRR2099107_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099107_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1379963
Sequences flagged as poor quality0
Sequence length47
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGCAAGAGCACATGGAAAGGGTTAAAGGCAGTCTGCAGGACTG19570.14181539649976124No Hit
CTGATGCAGCGATACTCGAGGTGGGCAAGGGTGTGTAGGTGGGGACG16950.12282938020802008No Hit
CTGATGCAATTCGTTTGGGTGGCGCACGCCTTTAATCCCAGCACTTG14970.10848116942265844No Hit
CTGATGCAACGGAGCTGAGGAGATTGAGACCATCCTGGCTAACACGG14240.10319117251694429No Hit
CTGCTGCTTAAATTACGGTTCGTCGCACCCGAGCCGATGCCGGTTGC13900.10072733834168017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGCAC231750.040.6983833
ATGCACG57600.040.5720564
GATGCAG302450.040.199353
ATGCAAG111450.040.0794564
TGCAGCG18800.040.017775
TGCATAG22300.039.987945
ATGCACC46650.039.944494
ATGCAGG86050.039.760344
ATGCAAC94100.039.7573934
ATGCATA84500.039.664844
TGCACGC18000.039.518615
ATGCAAT75650.039.4816474
GATGCAA449850.039.4497573
ATGCATG56200.039.4306564
TGCAACT21600.039.385745
CAGCGCG1250.039.3591657
TGCACGT9950.039.3509255
TGCAATA27100.039.183675
TGCATAC18750.039.1405035
TGCAACG25250.039.050665
Produced by FastQC (version 0.11.5)