FastQCFastQC Report
Thu 26 May 2016
SRR2099106_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099106_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321418
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGTCAGCAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27990.8708286405864015No Hit
GAACGTGAAATTCGTGGTAGGCACCTGTAATCCCAGTAACTTG15900.4946829362387919No Hit
GTACGTCAGTACTACAGTCCGACGATCGGGGAGTACGTCATTT12660.39387962092975504No Hit
GAACGTGAAATTCGGTGGTAGGCACCTGTAATCCCAGTAACTT6910.2149848483905693No Hit
GTACGTCAGAGACGGGCGGAGCCTAGGACTGAGGGCGATGGCT6810.21187363495510517No Hit
GTACGTCAACAGTTCGGGGTCGGGGCACGCCGGCCTCGCGGGC6360.19787317449551675No Hit
GTACGTCAGGGCAGGTGGCAGCCTCCAGGAGGCAGGGTCCTGC6170.19196186896813494No Hit
GCGCGTCAGGTCGGCCGACGCCGTGAAGCCGTCGATCACCGAG5580.17360570969889677No Hit
GTACGTCAAAAAGCTACAGTCCGACGATCTGGACGTACTACCT5390.16769440417151496No Hit
GTACGTCAGAGGCCTACAGTCTGACGATCATCCTGTACGTCAT5320.1655165547666901No Hit
GTACGTCACCCGAGTGAGGGCGAACTAGAATTAGTAGTGAGAA5250.16333870536186523No Hit
GAACGTCAGCAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5250.16333870536186523No Hit
GTACGTCAACAGGGGCAGAGCCACCCCAGTGTACCTCTTTGGC5240.16302758401831882No Hit
GTACGTCATGGACTGCGAATGGTGGAATCCACCTTCCCAACGA4770.14840488087163756No Hit
GGACGTTACATTTGCAACCCGGTAGTGCAGCCAGTGCCCACGA4320.1344044204120491No Hit
GTACGTCAGCCGCAGTTTTTTAGTGAAAAATGACGAAATCACT4250.13222657100722424No Hit
GTCCGTCAATAGCCAGTCCGACGATCCCACCGTACGCTCTGAT4120.1281819935411209No Hit
GTACGTCAGTGCGGGAAACTGAAAACCGAGGCATGCCTCATTT4110.1278708721975745No Hit
GTACGTCAGCCGCGTTTTTTAGTGAAAAATGACGAAATCACTA3860.12009283860891425No Hit
GTACGGCGCATGGACCCAGCGCGACCAATGGGTCTGTAGCTCA3780.11760386786054298No Hit
GTACGTCAACCTGGTCCGACGATCCCGTCGTACTGTAGAACTC3690.11480377576862527No Hit
CTACGTCACTCGCCTGGATCCCGTTATGCCGACGATTGGCGTA3600.11200368367670759No Hit
GTAGGACAAGCTAGCCATGCGACTCGGATCGATGTCTTCCCAC3580.11138144098961478No Hit
GTACGTCATCCGAGTCAGCTCTGCCGGCCCGAGAGGGGCGGGC3500.10889247024124349No Hit
GTACGTCACGATACAGTCCGACGATCATTGTGTACGTCGAGAT3420.1064034994928722No Hit
GTACGTCAGGCTCGTCCGACGATCCGAAGGTACGTCGGACTGT3380.10515901411868656No Hit
GTACGTCAAAAGAGGGCAAACCACGGAAGGTGGTTGGTTGCAG3360.10453677143159375No Hit
GTACGTCATCTGTGAGATCTGTGCTCCTGATGGCTGTTTATCC3350.10422565008804735No Hit
GTACGTCAGACACGTTCTCTGCATGTGTGTACAGATGATTGGA3310.1029811647138617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGC308.282348E-637.0071613
AGCGTCA200.00183821637.007168
ACTGCCG355.60598E-737.007168
ACCGCTA200.00183821637.007168
ACACCCG551.2732926E-1137.007168
GGACTAC251.2302458E-437.0071613
AATTGCG200.00183821637.007168
CGGATCC200.00183821637.0071613
AGGCACG452.6029738E-937.007168
ATCTTCG403.812056E-837.007168
AGCGAAC200.00183821637.007168
ACTAACG200.00183821637.007168
GTATACT308.282348E-637.0071614
AAGTGCG403.812056E-837.007168
ATACCTA200.00183821637.007168
ACCCAAT200.00183821637.007168
AAGTCGT200.00183821637.007168
TCCGGGT251.2302458E-437.007168
CGCGTCA850.037.0014042
GTCATAG2250.037.00145
Produced by FastQC (version 0.11.5)