FastQCFastQC Report
Thu 26 May 2016
SRR2099105_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099105_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278499
Sequences flagged as poor quality0
Sequence length42
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATGTTCAGTCGACTCACTATAGTGAGTCGTATTACGGCC18050.6481172284281093No Hit
ACGATGTCCCTCGGGAAGCCGAGGCAGGCTGATAATTTGAAG15580.5594275024326838No Hit
ACGATGTCCGCAAGGTTAGGAATAGGGTTTTAATGGTGAAGC14370.5159803087264228No Hit
ACGATGTCTGTCGCGCGCACCAGCGGCACCCCCGGGCGGTAC12040.4323175307631266No Hit
ACGATATATGCACGGTGAAACCCCGTCTCTACTAAAAATACA11780.4229817701320292No Hit
ACGATGTCCGCAGGTTAGGAATAGGGTTTTAATGGTGAAGCA11360.40790092603564104No Hit
ACGATGTCTCTGCGAGCACGTTGCCCTCGGACTTGGACATCT10280.3691216126449287No Hit
ACGATGTCTCCTGGGCTTTTTGCTTCTGTCCAAAATTACTTT9050.3249562835055063No Hit
ACGATGTCACGGGGCAAAGGTTGCAGCGAGCCAAGATCGTGC8760.31454331972466687No Hit
ACGATGTCCCTCGGGGAAGCCGAGGCAGGCTGATAATTTGAA8680.3116707779920215No Hit
ACGATGTCCAGGAATTTCTAAGGGCTTGGTCATCAGCAAAGT8510.3055666268101501No Hit
ACGATGTCCGTCGGCACCCGACAGGCGAAAAACGATCCGCCA8490.30484849137698883No Hit
ACGATGCCGCACGGCCAGCCCCAGATGGAATCAGCCCCCGGA6850.2459613858577589No Hit
ACGATGTCGGAGGTGAGAATATTAAGCAGCATTTATAGAGCT6200.22262198428001537No Hit
ACGATGTCCTCACCGACGATCGTGGACGATGTCTTTTAGGAT6200.22262198428001537No Hit
ACGACGTCCTCACCGACGATCGTGGACGATGTCTTTTAGGAT6020.2161587653815633No Hit
ACGATGTCAAACATTATAGGAATGAGAGTGGAAGCAGGGCAA5370.19281936380381978No Hit
ACGATGTCGATCCCTTAGGCATCCACACCTGACCACCAGAAT5200.18671521262194837No Hit
ACGATGTCCCTTAACACCATGCTTGGAGGGTGGGGGTAGAAA5040.18097012915665767No Hit
ACGATGCCTCTAGCCAGCACGGCGCCCGCAAGGTCGCCGGGC4800.17235250395872156No Hit
ACGATGTCCAAGTTCAGAAAGCATCTTGATTGAAAGATCCTT4770.17127530080897957No Hit
ACGATGTTCGCTTTTAGGAAGTTTTTGAAACAGCCCCTCTCT4720.1694799622260762No Hit
ACGATGTCGTGCGCATGCCTGGGGGAATGCCTGGTGGCTTCC4310.15475818584626877No Hit
ACGATGTCTAGGGTCAGGAGATCGAGACCGTCCTCGCTAACA4260.1529628472633654No Hit
ACGATGTCTAGGGGTCAGGAGATCGAGACCGTCCTCGCTAAC4160.1493721700975587No Hit
ACGATGTCGGTTGGTGGATCACCTGAGGTCAGGAGTTTGAGA4160.1493721700975587No Hit
ACGATGTCAATTCGACGATCGCGTACGATGTCGGACTGTAGA4150.14901310238097804No Hit
ACGATGTCCTCTCGAACCATTGGTGCGAGAGCTCGTGCGCGA3710.13321412285142853No Hit
ACGATGTCCGGGGCAAAATGGCTCCAACCTATTTTGCAAGAA3710.13321412285142853No Hit
ACGATGTCGGTAGATCCGCCCACCTCGGCCTCCCAAAGTGCT3610.12962344568562184No Hit
ACGATGTCGAGCATTGATTTTTCAATTGTTTTAGAAAAATCC3540.12710997166955715No Hit
ACGATGTCGGTCACTGAGGCCATGATTAAGAGGGACGGCCGG3510.12603276851981515No Hit
ACGAGCTCAGCCGCGGATCTGGCGTACTCTCATTGACATCTC3490.1253146330866538No Hit
ACGATGTCGTGTGGTGGCTCACACCTGTAATCCCAGCACTTT3450.12387836222033112No Hit
ACGATGTCGAGCGTCCGACGATCGCGGACGATGTCTTTTTTT3350.12028768505452443No Hit
ACGATGTCGCAAAGGACCAAGAAGAGGAAGAAGACCAAGGCC3330.11956954962136308No Hit
ACGATGTCCCGAACGATCTTCCCTACGAGTTTTTTTTTTTTT3220.11561980473897572No Hit
ACGATGTCTCTGGCGAGCACGTTGCCCTCGGACTTGGACATC3150.11310633072291103No Hit
ACGATGTCCGGGGTTAAATGGTGTGTGTGATGGACCGAATGC3080.11059285670684635No Hit
ACGATGTCAGGGATCTACGGCAGTAGCAACTTGGCCCGACCG3010.10807938269078166No Hit
GCGATGACAGTCAGGAAACTGGTTCAAGATCACGTCAAAATG2820.10125709607574891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCCG453.3815013E-936.02462412
GTTTACT200.002097320336.02462413
CGTCTTC200.002097320336.02462412
AAGGTTA1600.036.02462412
TAAATGC251.4407639E-436.02462413
TATACGA551.6370905E-1136.02462412
CGATATG200.002097320336.02462412
TCTGCCG453.3942342E-936.011687
CGGGTAG200.002101024136.011688
TCGCATT200.002101024136.011687
TCCGCGG404.8423317E-836.011687
TCCGCAC404.8423317E-836.011687
CGAGTGA200.002101024136.0116810
CACGGGG1950.036.011688
CGTGATT200.002101024136.011688
CATGACG404.8423317E-836.011689
GCACGGG200.002101024136.011689
CGCGCAT200.002101024136.011688
CGACCAA200.002101024136.011688
CCGCTTC200.002101024136.011689
Produced by FastQC (version 0.11.5)